HUP0002320A2 - Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same - Google Patents
Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the sameInfo
- Publication number
- HUP0002320A2 HUP0002320A2 HU0002320A HUP0002320A HUP0002320A2 HU P0002320 A2 HUP0002320 A2 HU P0002320A2 HU 0002320 A HU0002320 A HU 0002320A HU P0002320 A HUP0002320 A HU P0002320A HU P0002320 A2 HUP0002320 A2 HU P0002320A2
- Authority
- HU
- Hungary
- Prior art keywords
- dna
- integrated
- genome
- homologous recombination
- same
- Prior art date
Links
- 230000006801 homologous recombination Effects 0.000 title abstract 4
- 238000002744 homologous recombination Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 210000004962 mammalian cell Anatomy 0.000 title abstract 3
- 239000013598 vector Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 5
- 239000012634 fragment Substances 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 239000003550 marker Substances 0.000 abstract 2
- 239000013612 plasmid Substances 0.000 abstract 2
- 230000001131 transforming effect Effects 0.000 abstract 2
- 238000012216 screening Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Inserting DNA at a target site into the genome of a mammalian cell comprises: (i) transforming the cell with a marker plasmid (MP) containing these sequences: a DNA that is heterologous to the mammalian cell genome which, when integrated into the genome, provides a unique site for homologous recombination (HR); a DNA fragment encoding a portion of a selectable marker (SM) protein; and >=1 SM DNA (SM2) for the selection of cells having the MP integrated; (ii) selecting cells having the MP integrated into its genome; (iii) transforming the selected cells with a target plasmid (TP) containing: DNA that is identical to the unique region in the MP so that this region can combine by HR; DNA encoding a portion of the same SM as in the MP, where the active SM protein is only produced if the fragment is expressed in association with the fragment of the SM DNA in the MP; and (iv) selecting cells containing the TP integrated at the target site by screening for expression of the first SM protein. Also new is a vector system for inserting DNA at a target site comprising the MP and TP above.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/023,715 US5998144A (en) | 1997-03-14 | 1998-02-13 | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP0002320A2 true HUP0002320A2 (en) | 2000-11-28 |
| HUP0002320A3 HUP0002320A3 (en) | 2001-09-28 |
Family
ID=21816786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU0002320A HUP0002320A3 (en) | 1998-02-13 | 1998-03-09 | Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same |
Country Status (1)
| Country | Link |
|---|---|
| HU (1) | HUP0002320A3 (en) |
-
1998
- 1998-03-09 HU HU0002320A patent/HUP0002320A3/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0002320A3 (en) | 2001-09-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FD9A | Lapse of provisional protection due to non-payment of fees |