HUP0001737A1 - Methods and materials for making and using transgenic dicamba-degrading organisms - Google Patents
Methods and materials for making and using transgenic dicamba-degrading organismsInfo
- Publication number
- HUP0001737A1 HUP0001737A1 HU0001737A HUP0001737A HUP0001737A1 HU P0001737 A1 HUP0001737 A1 HU P0001737A1 HU 0001737 A HU0001737 A HU 0001737A HU P0001737 A HUP0001737 A HU P0001737A HU P0001737 A1 HUP0001737 A1 HU P0001737A1
- Authority
- HU
- Hungary
- Prior art keywords
- dicamba
- dna
- organisms
- ddo
- isolated
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 239000000463 material Substances 0.000 title abstract 4
- 230000009261 transgenic effect Effects 0.000 title abstract 4
- 108020004414 DNA Proteins 0.000 abstract 10
- 239000005504 Dicamba Substances 0.000 abstract 6
- IWEDIXLBFLAXBO-UHFFFAOYSA-N dicamba Chemical compound COC1=C(Cl)C=CC(Cl)=C1C(O)=O IWEDIXLBFLAXBO-UHFFFAOYSA-N 0.000 abstract 6
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 4
- 102000053602 DNA Human genes 0.000 abstract 2
- JDZSMXLTQNHBRF-UHFFFAOYSA-N Dichlozoline Chemical compound O=C1C(C)(C)OC(=O)N1C1=CC(Cl)=CC(Cl)=C1 JDZSMXLTQNHBRF-UHFFFAOYSA-N 0.000 abstract 2
- FKIKPQHMWFZFEB-UHFFFAOYSA-N 3,6-dichloro-2-hydroxybenzoic acid Chemical compound OC(=O)C1=C(O)C(Cl)=CC=C1Cl FKIKPQHMWFZFEB-UHFFFAOYSA-N 0.000 abstract 1
- 108010074122 Ferredoxins Proteins 0.000 abstract 1
- 108010044672 O-Demethylating Oxidoreductases Proteins 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000417 Oxygenases Proteins 0.000 abstract 1
- 102000004020 Oxygenases Human genes 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
An isolated DNA molecule is claimed which comprises a DNA sequence coding for a dicamba-degrading oxygenase (DDO). Also claimed are: (1) a DNA construct comprising a DNA sequence coding for a DDO operatively linked to expression control sequences; (2) an isolated and at least partially purified DDO; (3) an isolated DNA molecule comprising a DNA sequence coding for a dicamba-degrading ferredoxin (DDF); (4) a DNA construct comprising a DNA sequence coding for a DDF operatively linked to expression control sequences; (5) an isolated and at least partially purified DDF; (6) an isolated and at least partially purified dicamba-degrading reductase (DDR); (7) an isolated and at least partially purified dicamba-degrading O-demethylase (DDOD); (8) a transgenic host cell comprising DNA coding for a DDO operatively linked to expression control sequences; (9) a transgenic host cell comprising DNA coding for a DDF operatively linked to expression control sequences; (10) a transgenic plant or part of a plant comprising one or more cells comprising DNA coding for a DDO operatively linked to expression control sequences; (11) a method of decontaminating a material containing dicamba comprising applying a DDOD or a combination of a DDO, a DDF and a DDR to the material to degrade at least some of the dicamba in the material; (12) a method of selecting transformed plant cells comprising: (a) providing a population of plant cells; (b) transforming at least some of the plant cells in the population of plant cells with a DNA construct as in (1) or (4); and (c) growing the resulting population of plant cells in a culture medium containing dicamba at a concentration selected so that transformed plant cells will grow and untransformed plant cells will not grow; (13) a method of selecting transformed plants comprising: (a) providing a population of plants suspected of comprising a DNA construct as in (1) or (4); and (b) applying dicamba to the plants selected so that transformed plants will grow, and growth of untransformed plants will be inhibited; (14) a method of selecting, or screening for, transformed host cells, intact organisms, and parts of organisms, comprising: (a) providing a population of host cells, intact organisms or parts of organisms suspected of comprising a DNA construct as in (1) or (4); (b) contacting the host cells, intact organisms, or parts of organisms with dicamba; and (c) ascertaining the presence or level of fluorescence due to 3,6-dichlorosalicylic acid generated in transformed host cells, intact organisms, or parts of organisms as a result of the degradation of dicamba.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4294197P | 1997-04-04 | 1997-04-04 | |
| PCT/US1998/006589 WO1998045424A1 (en) | 1997-04-04 | 1998-04-03 | Methods and materials for making and using transgenic dicamba-degrading organisms |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| HUP0001737A1 true HUP0001737A1 (en) | 2000-11-28 |
| HUP0001737A3 HUP0001737A3 (en) | 2002-03-28 |
Family
ID=21924571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HU0001737A HUP0001737A3 (en) | 1997-04-04 | 1998-04-03 | Methods and materials for making and using transgenic dicamba-degrading organisms |
Country Status (1)
| Country | Link |
|---|---|
| HU (1) | HUP0001737A3 (en) |
-
1998
- 1998-04-03 HU HU0001737A patent/HUP0001737A3/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUP0001737A3 (en) | 2002-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA9A | Lapse of provisional patent protection due to relinquishment or protection considered relinquished |