HUE027997T2 - Pyridine and pyrazine derivative for the treatment of cf - Google Patents

Description

Description [0001] This invention relates to pyridine and pyrazine compounds, their preparation and use as pharmaceuticals.

[0002] Cystic fibrosis (CF) is a fatal genetic disease caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR), a protein kinase A (PKA)-activated epithelial anion channel involved in salt and fluid transport in multiple organs, including the lung. Most CF mutations either reduce the number of CFTR channels at the cell surface (e.g., synthesis or processing mutations) or impair channel function (e.g., gating or conductance mutations) or both. There are currently no approved therapies that target CFTR directly. The present invention discloses compounds which restore or enhance the function of mutant and/or wild type CFTR to treat cystic fibrosis, primary ciliary dyskinesia, chronic bronchitis, chronic obstructive pulmonary disease, asthma, respiratory tract infections, lung carcinoma, xerostomia and keratoconjunctivitis sire, or constipation (IBS, IBD, opioid induced).

[0003] In one aspect, the invention provides compounds according to Formula I:

I or pharmaceutically acceptable salts thereof, wherein: A is N or CR4a; R1 is H; C1-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -CVCA alkyl-C3-C8 cycloalkyl; C1-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; S02NR8R9; S02R10; S-C1-C8alkyl optionally substituted by one or more halogen atoms; S-C6-C14 aryl; CN; NR11R12; C(0)NR13R14; NR13S02R15; NR13C(0)R15, C02R15, -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; R2 is C-|-C4 haloalkyl; R3 and R4a are each independently FI or C.|-C8 alkyl optionally substituted by one or more halogen atoms; R4 is FI, or C1-C8 alkyl optional substituted with one or more halogen; R5 is -(CFI2)m-NR17R18, -(CFI2)m-OR’; C1-C8 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C02R15; -(C0-C4 alkyl)-C6-C14 aryl or -3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; R6 is C1-C8 alkyl optionally substituted by one or more halogen atoms; C3-C10 cycloalkyl; -CVCA alkyl-C3-C8 cycloalkyl; CrC8 alkoxy optionally substituted by one or more halogen atoms; OFI; CN; halogen; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; or R6 is FI, and R5 is -(CFI2)m-NR17R18, -(CFI2)m-OR\ C1-C8 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; or -(C0-C4 alkyl)-C02R15, wherein -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group groups are each optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R4 and R5 together form an oxo group (C=0) and R6 is CVCA alkyl optionally substituted by one or more halogen atoms; C.|-C4 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R5 and R6 together with the carbon atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; or R4 and R5 and R6 together with the carbon atoms to which they are bound form a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R is H, or C.|-C8 alkyl optional substituted with one or more halogen; m is 0, 1,2 or 3; R8, R11, R13 and R17 are each independently H, C.|-C8 alkyl optionally substituted by one or more halogen atoms, C3-C10 cycloalkyl or -(CrC4 alkyl)-C3-C8 cycloalkyl; R9, R10, R12, R14, R15, R16 and R18 are each independently H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R8 and R9, R11 and R12, R13 and R14, and R17 and R18 together with the nitrogen atom to which they are attached may form a 4 to 14 membered heterocyclic group optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, benzyl, O-benzyl, C^Cg alkyl optionally substituted by one or more OH groups or NH2 groups, C^Cg alkyl optionally substituted by one or more halogen atoms, C^Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21, NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, CN, N02, halogen or a 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; R19 and R21 are each independently H; C^Cg alkyl; C3-C8 cycloalkyl; CrC4 alkoxy-C1-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, C^Cg alkyl and C(0)C^-C& alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C-pCg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, C^Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, C.|-C4 alkoxy, C(0)NH2, C(0)NHC1-C6 alkyl or 0(0)Ν(0Γ06 alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, O and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C1-C6 alkyl; C^Cg alkyl optionally substituted by one or more halogen atoms; C^Cg alkoxy optionally substituted by one or more OH groups or C1-C4 alkoxy; and C(0)0C.|-Cg alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by C^Cg alkyl, C^Cg haloalkyl or C^Cg alkoxy.

[0004] Various embodiments of the invention are described herein. It will be recognized thatfeatures specified in each embodiment may be combined with other specified features to provide further embodiments.

[0005] In an embodiment of the invention as described anywhere herein, A is N.

[0006] In an embodiment of the invention as described anywhere herein, A is CR4a.

[0007] In an embodiment of the invention as described anywhere herein, R1 is selected from H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C.|-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; and NR11R12, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents.

[0008] In an embodiment of the invention as described anywhere herein, R1 is C1-C4 alkyl optional substituted by one or more halogen atoms. For example, -CH3 or CF3.

[0009] In an embodiment of the invention as described anywhere herein, R1 is C1-C4 alkoxy optional substituted by one or more halogen atoms. For example, -OCFI3 or -OCF3.

[0010] In an embodiment of the invention as described anywhere herein, R1 is aryl, wherein aryl is phenyl optionally substituted by one or more Z substituents, specific example are 4-fluorophenyl, 4-chloro-2-methylphenyl, or 2,4-dichlo-rophenyl.

[0011] In an embodiment of the invention as described anywhere herein, R1 is 6 membered heterocyclyl group, wherein 6 membered heterocyclyl group is pyridyl optionally substituted by one or more Z substituents, specific example is 1-methyl-4-pyridyl.

[0012] In an embodiment of the invention as described anywhere herein, R1 is Br, -CH3, -CF3, -OCH3, -OCF3, 4-fluorophenyl, 4-chloro-2-methylphenyl, or 2,4-dichlorophenyl.

[0013] In an embodiment of the invention as described anywhere herein, R2 is CF3CF2-, (CF3)2CFI-, CFI3-CF2-, CF3CF2-, CF3, CF2H-, CH3-CCI2-, CF3CFCCIH-, CBr3, CBr2H-CF3CF2CHCF3 or CF3CF2CF2CF2-.

[0014] In an embodiment of the invention as described anywhere herein, R2 is CF3.

[0015] In an embodiment of the invention as described anywhere herein, R3 is FI or methyl.

[0016] In a further embodiment of the invention as described anywhere herein, R4a is FI.

[0017] An embodiment of the invention, as defined above provides a compound, where R5 provides a heteroatom two carbons from the amide nitrogen, wherein the heteroatom is oxygen or nitrogen.

[0018] An embodiment of the invention as defined above provides a compound according to Formula I, wherein R4 is FI, C.|-C4 alkyl optionally substituted by one or more halogen atoms or not present; R5 is C.|-C4 alkoxy optionally substituted by one or more halogen atoms; -(CFI2)m-NR17R18 ; -(CFI2)m-OR’, or OFI; m is 0, or 1 ; R6 is CrC4 alkyl optionally substituted by one or more halogen atoms; CrC4 alkoxy optionally substituted by one or more halogen atoms; OFI; CN; halogen; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R4 and R5 together form an oxo group (C=0); or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R17 and R18 are each independently FI; or CrC4 alkyl optionally substituted by one or more halogen atoms.

[0019] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is halogen, C^-C4 alkyl optionally substituted by one or more halogen atoms, or C^-C4 alkoxy optionally substituted by one or more halogen atoms; R2 is CrC4 haloalkyl; R3 is H; R4 is H or Me; R4a is H; R5 is -(CH2)m-NR17R18; -(CH2)m-OR’; or OH; m is 0, or 1 ; R6 is CrC4 alkyl optionally substituted by one or more halogen atoms; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 6 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; and R17 and R18 are each independently H; or C^-C4 alkyl optionally substituted by one or more halogen atoms.

[0020] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is halogen, CVC4 alkyl optionally substituted by one or more halogen atoms, or CrC4 alkoxy optionally substituted by one or more halogen atoms; R2 is C-|-C4 haloalkyl; R3 is H; R4a is H; R4 and R5 together form an oxo group (C=0); and R6 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; C.|-C4 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents..

[0021] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; R2 is C.|-C4 haloalkyl; R3 is H; R4 is H or Me; R4a is H; R5 is -(CH2)m-NR17R18; -(CH2)m-OR’; or OH; m is 0, or 1 ; R6 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 6 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; and R17 and R18 are each independently H; or C.|-C4 alkyl optionally substituted by one or more halogen atoms.

[0022] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is C-|-C4 alkoxy optionally substituted by one or more halogen atoms; R2 is CrC4 haloalkyl; R3 is H; R4 is H or Me; R4a is H; R5 is -(CH2)m-NR17R18; -(CH2)m-OR; or OH; m is 0, or 1 ; R6 is C-|-C4 alkyl optionally substituted by one or more halogen atoms; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 6 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; and R17 and R18 are each independently H; or CrC4 alkyl optionally substituted by one or more halogen atoms.

[0023] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is CrC4 alkoxy optionally substituted by one or more halogen atoms;; R2 is C.|-C4 haloalkyl; R3 is H; R4 is H or Me; R4a is H; R5 is -NR17R18; or OH; R6 is C-|-C4 alkyl optionally substituted by one or more halogen atoms; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 6 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; and R17 and R18 are each independently H; or C.|-C4 alkyl optionally substituted by one or more halogen atoms.

[0024] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; R2 is C.|-C4 haloalkyl; R3 is H; R4 is H or Me; R4a is H; R5 is -NR17R18 ; or OH; R6 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; and R17 and R18 are each independently H; or C-|-C4 alkyl optionally substituted by one or more halogen atoms.

[0025] An embodiment of the invention as defined above provides compounds according to Formula I, wherein A is CR4a ; R1 is C.|-C4 alkoxy optionally substituted by one or more halogen atoms; R2 is C.|-C4 haloalkyl; R3 is H; R4 is H or Me; R4a is H; R5 is -NR17R18 ; or OH; R6 is C1-C4 alkyl optionally substituted by one or more halogen atoms; and R17 and R18 are each independently H; or C.|-C4 alkyl optionally substituted by one or more halogen atoms.

[0026] In an embodiment of the invention as described anywhere herein, wherein Z is independently OH, CrC4 alkyl optionally substituted by one or more OH groups or NH2 groups, CrC4 alkyl optionally substituted by one or more halogen atoms, C1-C4 alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR19R21, C(0)0R19, 0(O)R19, SR19, OR19, ON, N02, or halogen; R19 and R21 are each independently H; C1-C4 alkyl; C3-C6 cycloalkyl; or C1-C4 alkoxy-C1-C4 alkyl, wherein all alkyls are optionally substituted with halogens.

[0027] In an embodiment of the invention as described anywhere herein, wherein Z is independently OH, CrC4 alkyl optionally substituted by one or more OH groups or NH2 groups, CrC4 alkyl optionally substituted by one or more halogen atoms, C1-C4 alkoxy optionally substituted by one or more OH groups or 0Γ04 alkoxy, C(0)OR19, C(0)R19, OR19, ON, or halogen; R19 is H; C1-C4 alkyl; C3-C6 cycloalkyl; or C1-C4 alkoxy-C1-C4 alkyl, wherein all alkyl are optionally substituted with halogens.

[0028] In an embodiment of the invention as described anywhere herein, wherein Z is independently, CrC4 alkyl optionally substituted by one or more halogen atoms, CrC4 alkoxy or halogen.

[0029] Another embodiment of the invention as defined above provides compounds with substantially pure enantiomers with the R configuration.

[0030] Another embodiment of the invention as defined above provides compounds with substantially pure enantiomers with the S configuration.

[0031] Certain compounds of Formula I include compounds of Formula II:

or a pharmaceutically acceptable salt thereof, wherein A, R1, R2 and R3 have the definitions of Formula I and R101 is

[0032] In a further embodiment of Formula II of the invention herein, A is CR4a, wherein R4a is H.

[0033] In a further embodiment of Formula II of the invention herein, R1 is selected from H; C.|-C4 alkyl optionally substituted by one or more halogen atoms; C^-C4 alkoxy optionally substituted by one or more halogen atoms; halogen; C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; and NR11R12, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents.

[0034] In a further embodiment of Formula II of the invention wherein, R1 is CyC4 alkyl optional substituted by one or more halogen atoms, C^-C4 alkoxy optionally substituted by one or more halogen atoms; halogen; C6 aryl; or 6 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents.

[0035] In a further embodiment of Formula II of the invention wherein, R1 is CrC4 alkyl optional substituted by one or more halogen atoms, CrC4 alkoxy optionally substituted by one or more halogen atoms; or halogen.

[0036] In a further embodiment of Formula II of the invention herein, R3 is FI or methyl.

[0037] An embodiment of the invention as defined above provides compounds according to Formula II, wherein A is CR4a; R1 is halogen; R3 is H; R4a is H; R101 is or

[0038] An embodiment of the invention as defined above provides compounds according to Formula II, wherein A is CR4a; R1 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; R3 is H; R4a is H; R101 is or

[0039] An embodiment of the invention as defined above provides compounds according to Formula II, wherein A is CR4a; R1 is C.|-C4 alkoxy optionally substituted by one or more halogen atoms; R3 is H; R4a is H; R101 is or

[0040] An embodiment of the invention as defined above provides compounds according to Formula II, wherein A is CR4a; R1 is halogen, CVCA alkyl optionally substituted by one or more halogen atoms, or C^-CA alkoxy optionally substituted by one or more halogen atoms; R3 is H; R4a is H; R101 is or

[0041] An embodiment of the invention as defined above provides compounds according to Formula II, wherein A is CR4a; R1 is halogen, CVCA alkyl optionally substituted by one or more halogen atoms, or C^-CA alkoxy optionally substituted by one or more halogen atoms; R3 is H; R4a is H;

R101 is or

[0042] An embodiment of the invention as defined above provides compounds according to Formula II, wherein A is CR4a; R1 is halogen, C.|-C4 alkyl optionally substituted by one or more halogen atoms, or C.|-C4 alkoxy optionally substituted by one or more halogen atoms; R3 is H; R4a is H;

R101 is or [0043] Another embodiment of the invention as defined above provides compounds according to Formula I and Formula II, represented by 3-amino-6-bromo-N-(imidazo[1,2-a]pyridin-2-ylmethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1 -methyl-1 FI-imidazol-4-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1 -methyl-1 FI-pyrazol-3-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-N-(2-(4-fluorophenyl)-2-oxoethyl)-6-(1 -methyl-1 FI-indol-6-yl)-5-(trifluoromethyl)picolinamide; 3-amino-6-bromo-N-((1-methyl-1 FI-imidazol-2-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-(6-(3-(dimethylamino)propoxy)pyridin-3-yl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; (R) -3-amino-6-bromo-N-((4-methylpiperazin-2-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1-methyl-1 FI-imidazol-5-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-(3-(N,N-dimethylsulfamoyl)phenyl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)picolinamide; 3-amino-6-bromo-N-isobutyl-N-methyl-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1-methyl-1 FI-pyrazol-5-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; (3-amino-6-bromo-5-(trifluoromethyl)pyrazin-2-yl)(4-methylpiperazin-1-yl)methanone; 3-amino-6-bromo-N-(2-(pyridin-4-yl)ethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-N-(2-(4-fluorophenyl)-2-oxoethyl)-6-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-5-(trifluoromethyl)picolinamide; 3-amino-6-(4-carbamoyl-2-methylphenyl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)picolinamide; 3-amino-6-bromo-N-(2-(pyridin-3-yl)ethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-(3,4-dimethylphenyl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)picolinamide; 3-amino-N-benzyl-6-bromo-N-methyl-5-(trifluoromethyl)pyrazine-2-carboxamide; (S) -3-amino-6-bromo-N-((1-ethylpyrrolidin-2-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; or 3-amino-6-bromo-N-(imidazo[1,5-a]pyridin-1-ylmethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide.

[0044] Another embodiment of the invention asdefined above provides compounds according to Formula I, represented by 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; Methyl 3-(3-amino-6-bromo-5-(trifluoromethyl)picolinamido)propanoate; 3-Amino-N-(benzo[d]isoxazol-3-ylmethyl)-6-bromo-5-(trifluoromethyl) picolinamide; 3-Amino-6-(oxazol-2-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-6-bromo-N-(3,3,3-trifluoro-2-methoxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-amino-N-(2-hydroxy-3-methyl-2-(trifluoromethyl)butyl)-6-methoxy-5-(trifluoromethyl)picolinamide; 3-Amino-6-cyclopropyl-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-hydroxy-2-(trifluoromethyl)propyl)-5-(trifluoro methyl) picolinamide; 5-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-3-(trifluoromethyl)-2,4’-bipyridine-6-carboxamide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3-methyl-2-oxobutyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-fluoro-phenyl)-2-oxo-ethyl]-amide; 3-Amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(2-methoxy-phenyl)-ethyl]-amide; 3-Amino-6-(1-methyl-1 H-pyrazol-4-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5,6-bis(trifluoromethyl) pyrazine-2-carboxamide; N-(2-(1 H-imidazol-2-yl)propyl)-3-amino-6-bromo-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-Amino-6-bromo-N-(2-morpholinoethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; (S)-3-amino-6-ethoxy-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoro methyl)picolinamide; 3-Amino-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-N-(2-amino-3,3,3-trifluoro-2-methylpropyl)-6-methoxy-5-(trifluoromethyl) picolinamide; or 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methylpropyl)-5-(trifluoromethyl)picolinamide.

[0045] It is understood that any and all embodiments of the present invention may be taken in conjunction with any other embodiment to describe additional embodiments of the present invention. Furthermore, any elements of an embodiment are meant to be combined with any and all other elements from any of the embodiments to describe additional embodiments. It is understood by those skilled in the art that combinations of substituents where not possible are not an aspect of the present invention.

[0046] Especially preferred specific compounds of formula (I) or formula (II) are those described hereinafter in the Examples.

Definitions [0047] Terms used in the specification have the following meanings: "Optionally substituted" means the group referred to can be substituted at one or more positions by any one or any combination of the radicals listed thereafter. "Optionally substituted by one or more Z groups" denotes that the relevant group may include one or more substituents, each independently selected from the groups included within the definition of Z. Thus, where there are two or more Z group substituents, these may be the same or different. "Flalo" or "halogen", as used herein, may be fluorine, chlorine, bromine or iodine. "C.|-C8-Alkyr, as used herein, denotes straight chain or branched alkyl having 1-8 carbon atoms. If a different number of carbon atoms is specified, such as C6 orC3, then the definition is to be amended accordingly, such as "C^C^AIkyl" will represent methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl and tert-butyl. "C1-C8-Alkoxy", as used herein, denotes straight chain or branched alkoxy having 1-8 carbon atoms. If a different number of carbon atoms is specified, such as C6 or C3, then the definition is to be amended accordingly, such as "C.|-C4-Alkoxy" will represent methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy and tert-butoxy. "C^C^FIaloalkyl", as used herein, denotes straight chain or branched alkyl having 1-4 carbon atoms with at least one hydrogen substituted with a halogen. If a different number of carbon atoms is specified, such as C6 or C3, then the definition is to be amended accordingly, such as "C^C^FIaloalkyl" will represent methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl and tert-butyl that have at least one hydrogen substituted with halogen, such as where the halogen is fluorine: CF3CF2~, (CF^CH-, CH3-CF2-, CF3CF2~, CF3, CF2H-, CF3CF2CHCF3 or CF3CF2CF2CF2-. "C3-C15-Cycloalkyl group", as used herein, denotes a cycloalkyl group having 3-to 15-ring carbon atoms that is saturated or partially saturated, such as a C3-C8-cycloalkyl. Examples of C3'C15 -cycolalkyl groups include but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl or cyclooctyl or a bicyclic group, such as bicyclooctyl, bicyclononyl including indanyl and indenyl and bicyclodecyl. If a different number of carbon atoms is specified, such as C6, then the definition is to be amended accordingly. "aryl" or "C6‘C15 -Aromatic carbocyclic group", as used herein, denotes an aromatic group having 6-to 15-ring carbon atoms. Examples of C6'C15 -aromatic carbocyclic groups include, but are not limited to, phenyl, phenylene, benzen-etriyl, naphthyl, naphthylene, naphthalenetriyl or anthrylene. If a different number of carbon atoms is specified, such as C10, then the definition is to be amended accordingly. "4- to 8-Membered heterocyclic group", "5- to 6- membered heterocyclic group", "3- to 10-membered heterocyclic group", "3- to 14-membered heterocyclic group", "4- to 14-membered heterocyclic group" and "5- to 14-membered heterocyclic group", refers, respectively, to 4- to 8-membered, 5- to 6-membered, 3- to 10-membered, 3- to 14-membered, 4- to 14-membered and 5- to 14-membered heterocyclic rings containing at least one ring heteroatom selected from the group consisting of nitrogen, oxygen and sulphur, which may be saturated, partially saturated or unsaturated (aromatic). The heterocyclic group includes single ring groups, fused ring groups and bridged groups. Examples of such heterocyclic groups include, but are not limited to, furán, pyrrole, pyrrolidine, pyrazole, imidazole, triazole, isotriazole, tetrazole, thiadiazole, isothiazole, oxadiazole, pyridine, piperidine, pyrazine, oxazole, isoxazole, pyrazine, pyridazine, pyrimidine, piperazine, pyrrolidine, pyrrolidinone, morpholine, triazine, oxazine, tetrahyrofuran, tetrahydrothiophene, tetrahydrothiopyran, tetra hyd ropy ran, 1,4-dioxane, 1,4-oxathiane, indazole, quinoline, inda-zole, indole, 8-aza-bicyclo[3.2.1]octane orthiazole.

[0048] A second aspect of the invention provides a compound of Formula (I), (II) or (III) as defined anywhere herein for use as a pharmaceutical.

[0049] A further aspect of the invention provides a compound of Formula (I), (II) or (III) for use in the treatment of an inflammatory or allergic condition, particularly an inflammatory or obstructive airways disease or mucosal hydration. Such conditions include, for example, cystic fibrosis, primary ciliary dyskinesia, chronic bronchitis, chronic obstructive pulmonary disease, asthma, respiratory tract infections, lung carcinoma, xerostomia and keratoconjunctivitis sire, or constipation (IBS, IBD, opioid induced).

[0050] A still further aspect of the present invention provides for the use of a compound of formula (I), (II) or (III), as defined in any of the aforementioned embodiments, in free or pharmaceutically acceptable salt form, for the manufacture of a medicament for the treatment of an inflammatory or allergic condition, particularly an inflammatory or obstructive airways disease or mucosal hydration.

[0051] An embodiment of the present invention provides for the use of a compound of formula (I), (II) or (III), as defined in any of the aforementioned embodiments, in free or pharmaceutically acceptable salt form, for the manufacture of a medicament for the treatment of an inflammatory or allergic condition selected from cystic fibrosis, primary ciliary dyskinesia, chronic bronchitis, chronic obstructive pulmonary disease, asthma, respiratory tract infections, lung carcinoma, xerostomia and keratoconjunctivitis sire, or constipation (IBS, IBD, opioid induced).

[0052] An embodiment of the present invention provides method for the prevention or treatment of a CFTR mediated condition or disease comprising administering an effective amount of at least one compound as described herein to a subject in need of such treatment. Such CFTR mediated condition or disease are selected from cystic fibrosis, primary ciliary dyskinesia, chronic bronchitis, chronic obstructive pulmonary disease, asthma, respiratory tract infections, lung carcinoma, xerostomia and keratoconjunctivitis sire, or constipation (IBS, IBD, opioid induced).

[0053] Throughout this specification and in the claims that follow, unless the context requires otherwise, the word "comprise", or variations such as "comprises" or "comprising", should be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

[0054] As used herein, the term "pharmaceutically acceptable salts" refers to salts that retain the biological effectiveness and properties of the compounds of this invention and, which typically are not biologically or otherwise undesirable. In many cases, the compounds of the present invention are capable of forming acid and/or base salts by virtue of the presence of amino and/or carboxyl groups or groups similar thereto.

[0055] Pharmaceutically acceptable acid addition salts can be formed with inorganic acids and organic acids, e.g., acetate, aspartate, benzoate, besylate, bromide/hydrobromide, bicarbonate/carbonate, bisulfate/sulfate, camphorsul-fornate, chloride/hydrochloride, chlortheophyllonate, citrate, ethandisulfonate, fumarate, gluceptate, gluconate, glucur-onate, hippurate, hydroiodide/iodide, isethionate, lactate, lactobionate, laurylsulfate, malate, maleate, malonate, man-delate, mesylate, methylsulphate, naphthoate, napsylate, nicotinate, nitrate, octadecanoate, oleate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, polygalacturonate, propionate, stearate, succinate, sulfosalicylate, tartrate, tosylate and trifluoroacetate salts.

[0056] Inorganic acids from which salts can be derived include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid, and the like.

[0057] Organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, oxalic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, toluenesulfonic acid, and sulfosalicylic acid.

[0058] Pharmaceutically acceptable base addition salts can be formed with inorganic and organic bases.

[0059] Inorganic bases from which salts can be derived include, for example, ammonium salts and metalsfrom columns I to XII of the periodic table. In certain embodiments, the salts are derived from sodium, potassium, ammonium, calcium, magnesium, iron, silver, zinc, and copper; particularly suitable salts include ammonium, potassium, sodium, calcium and magnesium salts.

[0060] Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, basic ion exchange resins, and the like. Certain organic amines include isopropylamine, benzathine, cholinate, diethanolamine, diethylamine, lysine, meglumine, piperazine and tromethamine.

[0061] The pharmaceutically acceptable salts of the present invention can be synthesized from a parent compound, a basic or acidic moiety, by conventional chemical methods. Generally, such salts can be prepared by reacting free acid forms of these compounds with a stoichiometric amount of the appropriate base (such as Na, Ca, Mg, or K hydroxide, carbonate, bicarbonate or the like), or by reacting free base forms of these compounds with a stoichiometric amount of the appropriate acid. Such reactions are typically carried out in water or in an organic solvent, or in a mixture of the two. Generally, use of non-aqueous media like ether, ethyl acetate, ethanol, isopropanol, or acetonitrile is desirable, where practicable. Lists of additional suitable salts can be found, e.g., in "Remington’s Pharmaceutical Sciences", 20th ed., Mack Publishing Company, Easton, Pa., (1985); and in "Handbook of Pharmaceutical Salts: Properties, Selection, and Use" by Stahl and Wermuth (Wiley-VCH, Weinheim, Germany, 2002).

[0062] Furthermore, the compounds of the present invention, including their salts, can also be obtained in the form of their hydrates, or include other solvents used for their crystallization.

[0063] Compounds of the invention, i.e. compounds of formula (I), (II) or (III) that contain groups capable of acting as donors and/or acceptors for hydrogen bonds may be capable of forming co-crystals with suitable co-crystal formers. These co-crystals may be prepared from compounds of formula (I), (II) or (III) by known co-crystal forming procedures. Such procedures include grinding, heating, co-subliming, co-melting, or contacting in solution compounds of formula (I), (II) or (III) with the co-crystal former under crystallization conditions and isolating co-crystals thereby formed. Suitable co-crystal formers include those described in WO 2004/078163. Hence the invention further provides co-crystals comprising a compound of formula (I), (II) or (III).

[0064] As used herein, the term "isomers" refers to different compounds that have the same molecular formula but differ in arrangement and configuration of the atoms. Also as used herein, the term "an optical isomer" or "a stereoisomer" refers to any of the various stereo isomeric configurations which may exist for a given compound of the present invention and includes geometric isomers. It is understood that a substituent may be attached at a chiral center of a carbon atom. Therefore, the invention includes enantiomers, diastereomers or racemates of the compound. "Enantiomers" are a pair of stereoisomers that are non- superimposable mirror images of each other. A 1:1 mixture of a pair of enantiomers is a "racemic" mixture. The term is used to designate a racemic mixture where appropriate. "Diastereoisomers" are stereoisomers that have at least two asymmetric atoms, but which are not mirror-images of each other. The absolute stereochemistry is specified according to the Cahn- Ingold- Prelog R-S system. When a compound is a pure enantiomer the stereochemistry at each chiral carbon may be specified by either R or S. Resolved compounds whose absolute configuration is unknown can be designated (+) or (-) depending on the direction (dextro- or levorotatory) which they rotate plane polarized light at the wavelength of the sodium D line. Certain of the compounds described herein contain one or more asymmetric centers or axes and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)- or (S)-. The present invention is meant to include all such possible isomers, including racemic mixtures, optically pure forms and intermediate mixtures. Optically active (R)-and (S)- isomers may be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques. If the compound contains a double bond, the substituent may be E or Z configuration. If the compound contains a disubstituted cycloalkyl, the cycloalkyl substituent may have a cis- or trans-configuration. All tautomeric forms are also intended to be included.

[0065] Any asymmetric atom (e.g., carbon or the like) of the compound(s) of the present invention can be present in racemic or enantiomerically enriched, for example the (R)-, (S)- or (R,S)- configuration. In certain embodiments, each asymmetric atom has at least 50 % enantiomeric excess, at least 60 % enantiomeric excess, at least 70 % enantiomeric excess, at least 80 % enantiomeric excess, at least 90 % enantiomeric excess, at least 95 % enantiomeric excess, or at least 99 % enantiomeric excess in the (R)- or (S)- configuration. Substituents at atoms with unsaturated bonds may, if possible, be present in cis- {7)- or trans- (E)- form.

[0066] Accordingly, as used herein a compound of the present invention can be in the form of one of the possible isomers, rotamers, atropisomers, tautomers or mixtures thereof, for example, as substantially pure geometric (cis or trans) isomers, diastereomers, optical isomers (antipodes), racemates or mixtures thereof.

[0067] Any resulting mixtures of isomers can be separated on the basis of the physicochemical differences of the constituents, into the pure or substantially pure geometric or optical isomers, diastereomers, racemates, for example, by chromatography and/or fractional crystallization.

[0068] Any resulting racemates of final products or intermediates can be resolved into the optical antipodes by known methods, e.g., by separation of the diastereomeric salts thereof, obtained with an optically active acid or base, and liberating the optically active acidic or basic compound. In particular, a basic moiety may thus be employed to resolve the compounds of the present invention into their optical antipodes, e.g., by fractional crystallization of a salt formed with an optically active acid, e.g., tartaric acid, dibenzoyl tartaric acid, diacetyl tartaric acid, di-0,0’-p-toluoyl tartaric acid, mandelic acid, malic acid orcamphor-10-sulfonic acid. Racemic products can also be resolved by chiral chromatography, e.g., high pressure liquid chromatography (HPLC) using a chiral adsorbent.

[0069] Since the compounds of the invention are intended for use in pharmaceutical compositions it will readily be understood that they are each preferably provided in substantially pure form, for example at least 60% pure, more suitably at least 75% pure and preferably at least 85%, especially at least 98% pure (% are on a weight for weight basis). Impure preparations of the compounds may be used for preparing the more pure forms used in the pharmaceutical compositions; these less pure preparations of the compounds should contain at least 1 %, more suitably at least 5% and preferably from 10 to 59% of a compound of the invention.

[0070] Compounds of the present invention are either obtained in the free form, as a salt thereof, or as prodrug derivatives thereof.

[0071] When both a basic group and an acid group are present in the same molecule, the compounds of the present invention may also form internal salts, e.g., zwitterionic molecules.

[0072] Any formula given herein is also intended to represent unlabeled forms as well as isotopically labeled forms of the compounds. Isotopically labeled compounds have structures depicted by the formulas given herein except that one or more atoms are replaced by an atom having a selected atomic mass or mass number. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine, and chlorine, such as 2H, 3H, 11C, 13C, 14C, 15N, 18F31P, 32P, 35S, 36CI, 125l respectively. The invention includes various isotopically labeled compounds as defined herein, for example those into which radioactive isotopes, such as 3H, 13C, and 14C are present. Such isotopically labeled compounds are useful in metabolic studies (with 14C), reaction kinetic studies (with, for example 2H or 3H), detection or imaging techniques, such as positron emission tomography (PET) or single-photon emission computed tomography (SPECT) including drug or substrate tissue distribution assays, or in radioactive treatment of patients. In particular, an 18F or labeled compound may be particularly desirable for PET or SPECT studies. Isotopically labeled compounds of this invention can generally be prepared by carrying out the procedures disclosed in the schemes or in the examples and preparations described below by substituting a readily available isotopically labeled reagent for a non-isotopically labeled reagent.

[0073] Further, substitution with heavier isotopes, particularly deuterium (i.e., 2FI or D) may afford certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements or an improvement in therapeutic index. It is understood that deuterium in this context is regarded as a substituent of a compound of the formula (I), (II) or (III). The concentration of such a heavier isotope, specifically deuterium, may be defined by the isotopic enrichment factor. The term "isotopic enrichment factor" as used herein means the ratio between the isotopic abundance and the natural abundance of a specified isotope. If a substituent in a compound of this invention is denoted deuterium, such compound has an isotopic enrichment factor for each designated deuterium atom of at least 3500 (52.5% deuterium incorporation at each designated deuterium atom), at least 4000 (60% deuterium incorporation), at least 4500 (67.5% deuterium incorporation), at least 5000 (75% deuterium incorporation), at least 5500 (82.5% deuterium incorporation), at least 6000 (90% deuterium incorporation), at least 6333.3 (95% deuterium incorporation), at least 6466.7 (97% deuterium incorporation), at least 6600 (99% deuterium incorporation), or at least 6633.3 (99.5% deuterium incorporation).

[0074] Isotopically-labeled compounds of formula (I), (II) or (III) can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Preparations using an appropriate isotopically-labeled reagents in place of the non-labeled reagent previously employed.

[0075] Pharmaceutically acceptable solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically substituted, e.g. D20, d6-acetone, d6-DMSO.

Synthesis [0076] Generally, compounds according to Formula I, II or III can be synthesized by the routes described in Scheme 1,2 and 3 and the Examples.

[0077] When A is CFI the pyridinyl moiety may be synthesized according to the general scheme 1 shown below.

Scheme 1

[0078] When A is nitrogen, the pyrazine moiety may be synthesized according to the general scheme 2 shown below.

Scheme 2

[0079] The right hand side of the moiety is typically added via an amide formation reaction as shown below in general scheme 3.

Scheme 3

[0080] HATU (2-(1 H-7-Azabenzotriazol-1-yl)—1,1,3,3-tetramethyl uranium hexafluorophosphate Methanaminium) is a peptide coupling agent. A skilled artisan would understand that other coupling agents cold possibly work. The halogen group in the above schemes can be replaced with other groups by choosing the appropriate nucleophile and catalyst. Protection of the Aryl NH2 group may be required and is represented by P. The schemes 4 -7 below are some representative examples.

Scheme 4

Scheme 5

[0081] The skilled person will appreciate that the general synthetic routes detailed above show common reactions to transform the starting materials as required. The specific reaction conditions are not provided, but these are well known to those skilled in the art and appropriate conditions considered to be within the skilled person’s common general knowledge.

[0082] The starting materials are either commercially available compounds or are known compounds and can be prepared from procedures described in the organic chemistry art.

[0083] Compounds of formula (I), (II) or (III), in free form, may be converted into salt form, and vice versa, in a conventional manner understood by those skilled in the art. The compounds in free or salt form can be obtained in the form of hydrates or solvates containing a solvent used for crystallisation. Compounds of formula (I), (II) or (III) can be recovered from reaction mixtures and purified in a conventional manner. Isomers, such as stereoisomers, may be obtained in a conventional manner, e.g., by fractional crystallisation or asymmetric synthesis from correspondingly asymmetrically substituted, e.g., optically active, starting materials.

[0084] The compounds of formula (I), (II) or (III) can be prepared, e.g., using the reactions and techniques described below and in the Examples. The reactions may be performed in a solvent appropriate to the reagents and materials employed and suitable for the transformations being effected. It will be understood by those skilled in the art of organic synthesis that the functionality present on the molecule should be consistent with the transformations proposed. This will sometimes require a judgment to modify the order of the synthetic steps or to select one particular process scheme over another in order to obtain a desired compound of the invention.

[0085] The various substituents on the synthetic intermediates and final products shown in the following reaction schemes can be present in their fully elaborated forms, with suitable protecting groups where required as understood by one skilled in the art, or in precursor forms which can later be elaborated into their final forms by methods familiar to one skilled in the art. The substituents can also be added at various stages throughout the synthetic sequence or after completion of the synthetic sequence. In many cases, commonly used functional group manipulations can be used to transform one intermediate into another intermediate, or one compound of formula (I), (II) or (III) into another compound of formula (I), (II) or (III). Examples of such manipulations are conversion of an ester or a ketone to an alcohol; conversion of an ester to a ketone; interconversions of esters, acids and amides; alkylation, acylation and sulfonylation of alcohols and amines; and many others. Substituents can also be added using common reactions, such as alkylation, acylation, halogénation or oxidation. Such manipulations are well-known in the art, and many reference works summarize procedures and methods for such manipulations. Some reference works which gives examples and references to the primary literature of organic synthesis for many functional group manipulations, as well as other transformations commonly used in the art of organic synthesis are March’s Organic Chemistry, 5th Edition, Wiley and Chichester, Eds. (2001); Comprehensive Organic Transformations, Larock, Ed., VCH (1989); Comprehensive Organic Functional Group Transformations, Katritzky et al. (series editors), Pergamon (1995); and Comprehensive Organic Synthesis, Trost and Fleming (series editors), Pergamon (1991). It will also be recognized that another major consideration in the planning of any synthetic route in this field is the judicious choice of the protecting group used for protection of the reactive functional groups present in the compounds described in this invention. Multiple protecting groups within the same molecule can be chosen such that each of these protecting groups can either be removed without removal of other protecting groups in the same molecule, or several protecting groups can be removed using the same reaction step, depending upon the outcome desired. An authoritative account describing many alternatives to the trained practitioner is Greene and Wuts, Protective Groups in Organic Synthesis, Wiley and Sons (1999).

Pharmacological activity [0086] Flaving regard to their modulation of CFTR activity, compounds of formula (I), in free or pharmaceutically acceptable salt form, hereinafter alternately referred to as "agents of the invention", are useful in the treatment of conditions which respond to the modulation of CFTR activity, particularly conditions benefiting from mucosal hydration such as cystic fibrosis.

[0087] Diseases mediated by modulation of CFTR activity, include diseases associated with the regulation of fluid volumes across epithelial membranes. For example, the volume of airway surface liquid is a key regulator of mucociliary clearance and the maintenance of lung health. The modulation of CFTR activity will promote fluid accumulation on the mucosal side of the airway epithelium thereby promoting mucus clearance and preventing the accumulation of mucus and sputum in respiratory tissues (including lung airways). Such diseases include respiratory diseases, such as cystic fibrosis, primary ciliary dyskinesia, chronic bronchitis, chronic obstructive pulmonarydisease(COPD), asthma, respiratory tract infections (acute and chronic; viral and bacterial) and lung carcinoma. Diseases mediated by modulation of CFTR activity also include diseases other than respiratory diseases that are associated with abnormal fluid regulation across an epithelium, perhaps involving abnormal physiology of the protective surface liquids on their surface, e.g., Sjogren’s Syndrome, xerostomia (dry mouth) or keratoconjunctivitis sire (dry eye). Furthermore, modulation of CFTR activity in the kidney could be used to promote diuresis and thereby induce a hypotensive effect.

[0088] Treatment in accordance with the invention may be symptomatic or prophylactic.

[0089] Asthma includes intrinsic (non-allergic) asthma and extrinsic (allergic) asthma, mild asthma, moderate asthma, severe asthma, bronchitic asthma, exercise-induced asthma, occupational asthma and asthma induced following bacterial infection. Treatment of asthma is also to be understood as embracing treatment of subjects, e.g., of less than 4 or 5 years of age, exhibiting wheezing symptoms and diagnosed ordiagnosable as "wheezy infants", an established patient category of major medical concern and now often identified as incipient or early-phase asthmatics. (For convenience this particular asthmatic condition is referred to as "wheezy-infant syndrome".) [0090] Prophylactic efficacy in the treatment of asthma will be evidenced by reduced frequency or severity of symptomatic attack, e.g., of acute asthmatic or bronchoconstrictor attack, improvements in lung function or improved airways hyperreactivity. It may further be evidenced by reduced requirement for other, symptomatic therapy, i.e., therapy for or intended to restrict or abort symptomatic attack when it occurs, e.g., anti-inflammatory (e.g., cortico-steroid) or bron-chodilatory. Prophylactic benefit in asthma may, in particular, be apparent in subjects prone to "morning dipping". "Morning dipping" is a recognized asthmatic syndrome, common to a substantial percentage of asthmatics and characterized by asthma attack, e.g., between the hours of about 4-6 am, i.e., at a time normally substantially distantfrom any previously administered symptomatic asthma therapy.

[0091] Chronic obstructive pulmonary disease includes chronic bronchitis or dyspnea associated therewith, emphysema, as well as exacerbation of airways hyperreactivity consequent to other drug therapy, in particular, other inhaled drug therapy. The invention is also applicable to the treatment of bronchitis of whatever type or genesis including, e.g., acute, arachidic, catarrhal, croupus, chronic or phthinoid bronchitis.

[0092] Dry eye disease is characterized by a decrease in tear aqueous production and abnormal tearfilm lipid, protein and mucin profiles. There are many causes of dry eye, someofwhich include age, laser eye surgery, arthritis, medications, chemical/thermal burns, allergies and diseases, such as cystic fibrosis and Sjogren’s Syndrome. Increasing anion secretion via CFTR would enhance fluid transport from the corneal endothelial cells and secretory glands surrounding the eye to increase corneal hydration. This would help to alleviate the symptoms associated with dry eye disease.

[0093] Sjogren’s Syndrome is an autoimmune disease in which the immune system attacks moisture-producing glands throughout the body, including eye, mouth, skin, respiratory tissue, liver, vagina and gut. Symptoms include dry eye, dry mouth and dry vagina, as well as lung disease. The disease is also associated rheumatoid arthritis, systemic lupus, systemic sclerosis and polymypositis/dermatomyositis. Defective protein trafficking is believed to cause the disease, for which treatment options are limited. Modulators of CFTR activity may hydrate the various organs affected by the disease and help to alleviate the associated symptoms.

[0094] The suitability of CFTR activity modulators as a treatment of a disease benefiting from mucosal hydration may be tested by determining the movement of chloride ions in a suitable cell-based assay. For example single cells or confluent epithelia, endogenously expressing or engineered to overexpress CFTR can be used to assess channel function using electrophysiological techniques or ion flux studies. See methods described in: Hirsh et al., J Pharm Exp Ther(2004); Moody et al., Am J Physiol Cell Physiol (2005).

[0095] CFTR activity modulators, including the compounds of formula (I), are also useful as co-therapeutic agents for use in combination with other drug substances, such as anti-inflammatory, bronchodilatory, antihistamine or anti-tussive drug substances, particularly in the treatment of cystic fibrosis or obstructive or inflammatory airways diseases such as those mentioned hereinbefore, e.g., as potentiators of therapeutic activity of such drugs or as a means of reducing required dosaging or potential side effects of such drugs.

[0096] The compounds of Formula (I), (II) or (III) may be mixed with the other drug substance in a fixed pharmaceutical composition or it may be administered separately, before, simultaneously with or after the other drug substance.

[0097] Accordingly, the invention includes as a further aspect a combination of a CFTR activity modulatorwith osmotic agents (hypertonic saline, dextran, mannitol, Xylitol), ENaC blockers, an anti-inflammatory, bronchodilatory, antihistamine, anti-tussive, antibiotic and/or DNase drug substance, wherein the CFTR activity modulator and the further drug substance may be in the same or different pharmaceutical composition.

[0098] Suitable antibiotics include macrolide antibiotics, e.g., tobramycin (TOBI™).

[0099] Suitable DNase drug substances include dornase alfa (Pulmozyme™), a highly-purified solution of recombinant human deoxyribonuclease I (rhDNase), which selectively cleaves DNA. Dornase alfa is used to treat cystic fibrosis.

[0100] Other useful combinations of CFTR activity modulators with anti-inflammatory drugs are those with antagonists of chemokine receptors, e.g., CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-6, CCR-7, CCR-8, CCR-9 and CCR10, CXCR1, CXCR2, CXCR3, CXCR4, CXCR5, particularly CCR-5 antagonists, such as Schering-Plough antagonists SC-351125, SCFI-55700 and SCFI-D; Takeda antagonists, such as A/-[[4-[[[6,7-dihydro-2-(4-methyl-phenyl)-5/-/-benzo-cy-clohepten-8-yl]carbonyl]amino]phenyl]-methyl]tetrahydro-/\/,/\/-dimethyl-2/-/-pyran-4-amin-ium chloride (TAK-770); and CCR-5 antagonists described in USP 6,166,037 (particularly claims 18 and 19), WO 00/66558 (particularly claim 8), WO 00/66559 (particularly claim 9), WO 04/018425 and WO 04/026873.

[0101] Suitable anti-inflammatory drugs include steroids, in particular, glucocorticosteroids, such as budesonide, bec-lamethasone dipropionate, fluticasone propionate, ciclesonide or mometasone furoate, or steroids described in WO 02/88167, WO 02/12266, WO 02/100879, WO 02/00679 (especially those of Examples 3, 11, 14, 17, 19, 26, 34, 37, 39, 51, 60, 67, 72, 73, 90, 99 and 101), WO 03/35668, WO 03/48181, WO 03/62259, WO 03/64445, WO 03/72592, WO 04/39827 and WO 04/66920; non-steroidal glucocorticoid receptor agonists, such as those described in DE 10261874, WO 00/00531, WO 02/10143, WO 03/82280, WO 03/82787, WO 03/86294, WO 03/104195, WO 03/101932, WO 04/05229, WO 04/18429, WO 04/19935 and WO 04/26248; LTD4 antagonists, such as montelukast and zafirlukast; PDE4 inhibitors, such as cilomilast (Ariflo® GlaxoSmithKline), Roflumilast (Byk Gulden),V-11294A (Napp), BAY19-8004 (Bayer), SCFI-351591 (Schering-Plough), Arofylline (Almirall Prodesfarma), PD189659/PD168787 (Parke-Davis), AWD-12-281 (Ásta Medica), CDC-801 (Celgene), SelCID(TM) CC-10004 (Celgene), VM554/UM565 (Vernalis), T-440 (Tan-abe), KW-4490 (Kyowa Hakko Kogyo), and those disclosed in WO 92/19594, WO 93/19749, WO 93/19750, WO 93/19751, WO 98/18796, WO 99/16766, WO 01/13953, WO 03/104204, WO 03/104205, WO 03/39544, WO 04/000814, WO 04/000839, WO 04/005258, WO 04/018450, WO 04/018451, WO 04/018457, WO 04/018465, WO 04/018431, WO 04/018449, WO 04/018450, WO 04/018451, WO 04/018457, WO 04/018465, WO 04/019944, WO 04/019945, WO 04/045607 and WO 04/037805; adenosine A2B receptor antagonists such as those described in WO 02/42298; and beta-2 adrenoceptor agonists, such as albuterol (salbutamol), metaproterenol, terbutaline, salmeterol fenoterol, pro-caterol, and especially, formoterol, carmoterol and pharmaceutically acceptable salts thereof, and compounds (in free or salt or solvate form) of formula (I) of WO 0075114, which document is incorporated herein by reference, preferably compounds of the Examples thereof, especially indacaterol and pharmaceutically acceptable salts thereof, as well as compounds (in free or salt or solvate form) of formula (I) of WO 04/16601, and also compounds of EP 1440966, JP 05025045, WO 93/18007, WO 99/64035, USP 2002/0055651, WO 01/42193, WO 01/83462, WO 02/66422, WO 02/70490, WO 02/76933, WO 03/24439, WO 03/42160, WO 03/42164, WO 03/72539, WO 03/91204, WO 03/99764, WO 04/16578, WO 04/22547, WO 04/32921, WO 04/33412, WO 04/37768, WO 04/37773, WO 04/37807, WO 04/39762, WO 04/39766, WO 04/45618, WO 04/46083, WO 04/80964, WO 04/108765 and WO 04/108676.

[0102] Suitable bronchodilatory drugs include anticholinergic or antimuscarinic agents, in particular, ipratropium bromide, oxitropium bromide, tiotropium salts and CFIF 4226 (Chiesi), and glycopyrrolate, but also those described in EP 424021, USP 3,714,357, USP 5,171,744, WO 01/04118, WO 02/00652, WO 02/51841, WO 02/53564, WO 03/00840, WO 03/33495, WO 03/53966, WO 03/87094, WO 04/018422 and WO 04/05285.

[0103] Suitable dual anti-inflammatory and bronchodilatory drugs include dual beta-2 adrenoceptor agonist/muscarinic antagonists such as those disclosed in USP 2004/0167167, WO 04/74246 and WO 04/74812.

[0104] Suitable antihistamine drug substances include cetirizine hydrochloride, acetaminophen, clemastine fumarate, promethazine, loratidine, desloratidine, diphenhydramine and fexofenadine hydrochloride, activastine, astemizole, aze-lastine, ebastine, epinastine, mizolastine and tefenadine, as well as those disclosed in JP 2004107299, WO 03/099807 and WO 04/026841.

[0105] In accordance with the foregoing, the invention also provides as a further aspect a method for the treatment of a condition responsive to modulation of CFTR activity, e.g., diseases associated with the regulation of fluid volumes across epithelial membranes, particularly an obstructive airways disease, which comprises administering to a subject, particularly a human subject, in need thereof a compound of formula (I), (II) or (III), in free form or in the form of a pharmaceutically acceptable salt.

[0106] In another aspect the invention provides a compound of formula (I), (II) or (III), in free form or in the form of a pharmaceutically acceptable salt, for use in the manufacture of a medicament for the treatment of a condition responsive to modulation of CFTR activity, particularly an obstructive airways disease, e.g., cystic fibrosis and COPD.

[0107] The agents of the invention may be administered by any appropriate route, e.g. orally, e.g., in the form of a tablet or capsule; parenterally, e.g., intravenously; by inhalation, e.g., in the treatment of an obstructive airways disease; intranasally, e.g., in the treatment of allergic rhinitis; topically to the skin; or rectally. In a further aspect, the invention also provides a pharmaceutical composition comprising a compound of formula (I), in free form or in the form of a pharmaceutically acceptable salt, optionally together with a pharmaceutically acceptable diluent or carrier therefor. The composition may contain a co-therapeutic agent, such as an anti-inflammatory, broncho-dilatory, antihistamine or antitussive drug as hereinbefore described. Such compositions may be prepared using conventional diluents or excipients and techniques known in the galenic art. Thus oral dosage forms may include tablets and capsules. Formulations for topical administration may take the form of creams, ointments, gels or transdermal delivery systems, e.g., patches. Compositions for inhalation may comprise aerosol or other atomizable formulations or dry powder formulations.

[0108] When the composition comprises an aerosol formulation, it preferably contains, e.g., a hydro-fluoro-alkane (HFA) propellant, such as HFA134a or HFA227 or a mixture of these, and may contain one or more co-solvents known in the art, such as ethanol (up to 20% by weight), and/or one or more surfactants, such as oleic acid orsorbitan trioleate, and/or one or more bulking agents, such as lactose. When the composition comprises a dry powder formulation, it preferably contains, e.g., the compound of formula (I), (II) or (III) having a particle diameter up to 10 microns, optionally together with a diluent or carrier, such as lactose, of the desired particle size distribution and a compound that helps to protect against product performance deterioration due to moisture, e.g., magnesium stearate. When the composition comprises a nebulised formulation, it preferably contains, e.g., the compound of formula (I), (II) or (III) either dissolved, or suspended, in a vehicle containing water, a co-solvent, such as ethanol or propylene glycol and a stabilizer, which may be a surfactant.

[0109] Further aspects of the invention include: (a) a compound of formula (I), (II) or (III) in inhalable form, e.g., in an aerosol or other atomisable composition or in inhalable particulate, e.g., micronised form; (b) an inhalable medicament comprising a compound of formula (I), (II) or (III) in inhalable form; (c) a pharmaceutical product comprising a compound of formula (I) in inhalable form in association with an inhalation device; and (d) an inhalation device containing a compound of formula (I), (II) or (III) in inhalable form.

[0110] Dosages of compounds of formula (I), (II) or (III) employed in practicing the present invention will of course vary depending, e.g., on the particular condition to be treated, the effect desired and the mode of administration. In general, suitable daily dosages for administration by inhalation are of the order of 0.005-10 mg, while for oral administration suitable daily doses are of the order of 0.05-100 mg.

Pharmaceutical Use and Assay [0111] Compounds of formula (I), (II) or (III) and their pharmaceutically acceptable salts, hereinafter referred to alternatively as "agents of the invention", are useful as pharmaceuticals. In particular, the compounds are suitable CFTR activity modulators and may be tested in the following assays.

Membrane potential assay [0112] CFTR activity can be quantified by measuring the transmembrane potential. The means for measuring the transmembrane potential in a biological system can employ a number of methods including electrophysiological and optical fluorescence-based membrane potential assays.

[0113] The optical membrane potential assay utilises a negatively charged potentiometric dye, such as the FLIPR membrane potential dye (FMP) (see Baxter DF, Kirk M, Garcia AF, Raimondi A, Flolmqvist MFI, Flint KK, Bojanic D, Distefano PS, Curtis R, Xie Y. Ά novel membrane potential-sensitive fluorescent dye improves cell-based assays for ion channels.’ J Biomol Screen. 2002 Feb;7(1 ):79-85) which when extracellular is bound to a quenching agent. Upon cellular depolarisation the negatively charged dye redistributes to the intracellular compartment, unbinding from the membrane impermeant quench agent, yielding an increase in fluorescence. This change in fluorescence is proportional to the change in transmembrane potential which can result from the activity of CFTR. The changes in fluorescence can be monitored in real time by an appropriately equipped fluorescence detector such as the FLIPR (fluorometric imaging plate reader) in 96 or 384-well microtitre plates.

Cell culture: [0114] Chinese hamster ovary (CFIO) cells stably expressing the AF508-CFTR channel were used for membrane potential experiments. Cells were maintained at 37 °C in 5% v/v C02 at 100% humidity in Modified Eagles medium (MEM) supplemenetd with 8% v/v foetal calf serum, 100μg/ml methotrexate and 100U/ml penicillin/streptomycin. Cells were grown in 225 cm2 tissue culture flasks. For membrane potential assays cells were seeded into 96 well plates at 40,000 cells per well, allowed to adhere and then maintained at 26 °C for 48h to facilitate channel insertion.

Potentiator assay: [0115] The membrane potential screening assay utilised a low chloride ion containing extracellular solution (~5mM) combined with a double addition protocol. The first addition was of buffer with or without test compound followed 5 minutes later by an addition offorskolin (1-20 μΜ) - this protocol favours maximum chloride efflux in response to AF508-CFTR activation. The AF508-CFTR mediated chloride ion efflux leads to a membrane depolarisation which is optically monitored by the FMP dye.

Solutions:

[0116] Low chloride extracellular (mM): 120 Na-gluconate, 1.2 CaCI2, 3.3 KH2P04, 0.8 K2HP04, 1.2 MgCI2, 10.0 D-glucose, 20.0 HEPES, pH 7.4 with NaOH FMP dye: made up as per manufacturers’ instructions in low chloride extracellular solution detailed above, at 10x final concentration, and stored as 1 mL aliquots at -20°C. lonWorks Quattro assay: [0117] CFTR activity can also be quantified electrophysiologically using the whole-cell configuration of the patch clamp technique (Hamill et al Pflügers Acrhive 1981). This assay directly measures the currents associated with chloride flow through CFTR channels whilst either maintaining or adjusting the transmembrane voltage. This assay can use either single glass micropipettes or parallel planar arrays to measure CFTR activity from native or recombinant cell systems. Currents measured using parallel planar arrays can be quantified using an appropriately equipped instrument such as the lonWorks Quattro (Molecular Devices) or the Qpatch (Sophion). The Quattro system can measure CFTR currents from either a single cell per recording well (HT configuration) or alternatively from a population of 64 cells per well (Population Patch Clamp PPC) (Finkel A, Wittel A, Yang N, Handran S, Hughes J, Costantin J. ’Population patch clamp improves data consistency and success rates in the measurement of ionic currents.’ J Biomol Screen. 2006 Aug;11(5):488-96).

Cell culture: [0118] Chinese hamsterovary (CHO) cells stably expressing the AF508-CFTR channel were used for lonWorks Quattro experiments. Cells were maintained at 37 °C in 5% v/v C02 at 100% humidity in D-MEM supplemented with 10 % (v/v) FCS, 100 U/mL Penicillin/Streptomycin, 1 % (v/v) NEAA, 1 mg/mIZeocin and 500 ug/ml Hygromycin B. For experiments cells were grown in 225 cm2 tissue culture flasks until near confluence and then cultured at 26 °C for 48-72h to facilitate channel insertion. Cells were removed from the flask and resuspended in either extracellular recording solution for immediate experimentation or alternatively in growth medium supplemented with 10% v/v DMSO and frozen to -80°C as 1-2 mL aliquots for use at a later date.

Potentiator assay: [0119] Cells, at a density of 1.5-3 million per mL, were placed on the Quattro system, added to the planar patch array and seals allowed to establish for 5-10 mins. After assessing seal resistances (commonly >50 ΜΩ), whole-cell access was obtained by perforation with 100 μς/ιτιΙ- amphotericin B. Baseline currents were measured by a pre-compound scan obtained by application of a voltage ramp from -100 to +100 mV. This was followed by addition of either buffer or test compound diluted in the extracellular solution supplemented with 20 μΜ forskolin, to each of the 384 wells of the planar parch array. After incubation step (5-20 minutes) the post-compound currents were measured again by application of a voltage ramp from -100 to +100 mV. The difference in currents between the pre- and post-compound scans defined the efficacy of CFTR potentiation.

Solutions:

[0120] Extracellular solution (ECS) : 145 mM NaCI, 4 mM CsCI, 5 mM D-glucose, 10 mM TES, 1 mM CaCI2, 1 mM MgCI2, pH 7.4 NaOH

Intracellular buffer (ICS): 113 mM L-Aspartic acid, 113 mM CsOH, 27 mM CsCI, 1 mM NaCI, 1 mM MgCI2, 1 mM EGTA, 10 mM TES. pH 7.2 with CsOH. Filter sterilized before use.

Ion transport assay: [0121] Another method to measure CFTR function is Ussings chambershort circuit current measurement. Engineered or native epithelial cells are grown to confluent monolayer on a semi-permeable filter and sandwiched between two perspex blocks. The flow of chloride ions via CFTR from one side of the epithelia to the other can be quantified by measuring the flow of current whilst maintaining the transepithelial potential at OmV. This is achieved using KCI filled agar-based electrodes to both clamp the cellular monolayer and measure the flow of currents.

Cell culture: [0122] FRT cells stably expressing AF508-CFTR were cultured on plastic in Coon’s modified F-12 medium supplemented with 32mM NaHC03,10% v/vfetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin and 30 μg/mL hygromycin B as the growth medium. For Ussing chamber experiments, the cells were grown as polarized epithelia on Snapwell permeable support inserts (500000 cells/insert in growth medium) and cultured for 7 to 9 days. The inserts were fed with fresh Coon’s modified F-12 growth medium every 48 hours, and 24 hours prior to Ussing chamber experiment. To increase the AF508 CFTR protein expression at the cell surface, plates were incubated at 27°C for48h before performing an Ussing chamber experiment.

Potentiator assay: [0123] Fischer Rat Thyroid (FRT) epithelial cells, stably expressing human AF508-CFTR were used as monolayer cultures on permeable supports. Cl-current was measured using the short circuit current technique, under an imposed basolateral to apical Cl- gradient in Ussing chambers. To measure stable Chcurrents, FRT cells were cultured for48h at 27°C to facilitate the insertion of AF508 CFTR into the plasma membrane. Ussing chamber studies were likewise conducted at 27°C. Under these conditions, the effects of cumulative additions of test compounds on AF508 CFTR currents could be quantitated with both potency and efficacy endpoints. Compounds were added to both the apical and basloalteral sides subsequent to addition of 10μΜ forskolin. Efficacy of compounds was compared to a known potentiator such as gensitein.

Solutions: [0124] Basolateral Ringer solution (mM): 126 NaCI, 24 NaHCOs, 0.38 KH2P04, 2.13 K2HP04, 1 MgS04, 1 CaCI2and 10 glucose.

[0125] Apical Ringer solution (mM): 140 Na-gluconate, 1 MgS04, 2 CaCI2, 1 HCI, 10 glucose and 24 NaHC03.

[0126] Compounds can also be tested for their ability to stimulate insertion of AF508 CFTR into the cell membrane using the above assays. For these assays the protocols were identical other than cells were not cultured at low temperature (26 or 27°C) but instead incubated with test compounds for 12-24 h prior to assay.

[0127] Compounds of the Examples, herein below, generally have EC50 values in the data measurements described above below 10 μΜ. Table 1 provides a list of representative compounds with their EC50 value.

Table 1.

[0128] Compounds listed below are within the scope of the broadest claim and the CFTR EC50 values in the data measurements described above were above 5μΜ: 3-amino-6-bromo-N-(imidazo[1,2-a]pyridin-2-ylmethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1-methyl-1H-imidazol-4-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 2- (3-amino-6-bromo-5-(trifluoromethyl)picolinamido)acetic acid; 3- amino-6-bromo-N-((1 -methyl-1 H-pyrazol-3-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-N-(2-(4-fluorophenyl)-2-oxoethyl)-6-(1 -methyl-1 H-indol-6-yl)-5-(trifluoromethyl)picolinamide; 3-amino-6-bromo-N-((1-methyl-1 H-imidazol-2-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 6-((3H-[1,2,3]triazolo[4,5-b]pyridin-3-yl)oxy)-3-(2,5-dimethyl-1 H-pyrrol-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-5-(trifluoromethyl)picolinamide; 3-amino-6-(6-(3-(dimethylamino)propoxy)pyridin-3-yl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)pyra- zine-2-carboxamide; (R) -3-amino-6-bromo-N-((4-methylpiperazin-2-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1-methyl-1 H-imidazol-5-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-(3-(N,N-dimethylsulfamoyl)phenyl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)picolinamide; 3-amino-6-bromo-N-isobutyl-N-methyl-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-bromo-N-((1-methyl-1 H-pyrazol-5-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 6-bromo-3-(methylamino)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; (3-amino-6-bromo-5-(trifluoromethyl)pyrazin-2-yl)(4-methylpiperazin-1-yl)methanone; 3-amino-6-bromo-N-(2-(pyridin-4-yl)ethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-N-(2-(4-fluorophenyl)-2-oxoethyl)-6-(1-oxo-1,2,3,4-tetrahydroisoquinolin-6-yl)-5-(trifluoromethyl)picolina-mide; 3-amino-6-(4-carbamoyl-2-methylphenyl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)picolinamide; 3-amino-6-bromo-N-(2-(pyridin-3-yl)ethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-(3,4-dimethylphenyl)-N-(2-(4-fluorophenyl)-2-oxoethyl)-5-(trifluoromethyl)picolinamide; 3-amino-N-benzyl-6-bromo-N-methyl-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-amino-6-hydroxy-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-amino-6-hydroxy-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; (3-amino-6-bromo-5-(trifluoromethyl)pyrazin-2-yl)(4-methyl-3-phenylpiperazin-1-yl)methanone; (S) -3-amino-6-bromo-N-((1-ethylpyrrolidin-2-yl)methyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; and 3-amino-6-bromo-N-(imidazo[1,5-a]pyridin-1-ylmethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide.

[0129] The invention is illustrated by the following Examples.

Examples

General Conditions: [0130] Mass spectra were run on LC-MS systems using electrospray ionization. These were either Agilent 1100 HPLC/Micromass Platform Mass Spectrometer combinations or Waters Acquity UPLC with SQD Mass Spectrometer.

[M+H]+ refers to mono-isotopic molecular weights.

[0131] NMR spectra were run on open access Bruker AVANCE 400 NMR spectrometers using ICON-NMR. Spectra were measured at 298K and were referenced using the solvent peak.

[0132] Optical rotations were measured at589nm and 546nm using an Optical activity AA-1000 polariméter at 21 °C.

[0133] The following examples are intended to illustrate the invention and are not to be construed as being limitations thereon. Temperatures are given in degrees centigrade. If not mentioned otherwise, all evaporations are performed under reduced pressure, preferably between about 15 mm Hg and 100 mm Hg (= 20-133 mbar). The structure of final products, intermediates and starting materials is confirmed by standard analytical methods, e.g., microanalysis and spectroscopic characteristics, e.g., MS, IR, and NMR. Abbreviations used are those conventional in the art. If not defined, the terms have their generally accepted meanings.

Abbreviations: [0134] app apparent ATP adenosine 5’-triphosphate BINAP racemic 2,2’-bis(diphenylphosphino)-1,1 ’-binaphthyl BOC tertiary butyl carboxy br broad d doublet dd doublet of doublets DCM dichloromethane DIEA diethylisopropylamine DIPEA diisopropylethylamine DMF N,N-dimethylformamide DMSO dimethylsulfoxide DTT dithiothreitol ESI electrospray ionization

EtOAc ethyl acetate eq equivalent h hour(s) HATU 2-(7-Aza-1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate HPLC high pressure liquid chromatography IR infrared spectroscopy LCMS liquid chromatography and mass spectrometry

MeOH methanol MS mass spectrometry MW microwave m multiplet min minutes ml milliliter(s) m/z mass to charge ratio NMR nuclear magnetic resonance ppm parts per million PS polymer supported rac racemic RT room temperature

Rt retention time s singlet SCX-2 strong cation exchange (e.g. Isolute® SCX-2 columns from Biotage) t triplet TEA triethylamine TFA trifluoroacetic acid THF tetrahydrofuran [0135] Referring to the examples that follow, compounds of the preferred embodiments were synthesized using the methods described herein, or other methods, which are known in the art.

[0136] The various starting materials, intermediates, and compounds of the preferred embodiments may be isolated and purified, where appropriate, using conventional techniques such as precipitation, filtration, crystallization, evaporation, distillation, and chromatography. Unless otherwise stated, all starting materials are obtained from commercial suppliers and used without further purification. Salts may be prepared from compounds by known salt-forming procedures.

[0137] It should be understood that the organic compounds according to the preferred embodiments may exhibit the phenomenon of tautomerism. As the chemical structures within this specification can only represent one of the possible tautomeric forms, it should be understood that the preferred embodiments encompasses any tautomeric form of the drawn structure.

[0138] If not indicated otherwise, the analytical HPLC conditions are as follows:

Method 10minLC_v001

Column Waters BEH C18 100x2.1 mm, 1.7 μίτι

Column Temp. 50 °C

Eluents A: H20, B: acetonitrile, both containing 0.1 % TFA

Flow Rate 0.7 ml/min

Gradient 0.25 min 5% B; 5% to 95% B in 7.75 min, 1.00 min 95% B

Method 10minLC_v002

Column Waters BEH C18 50x2.1 mm, 1.7 μίτι

Column Temperature 50 °C

Eluents A: H20, B: methanol, both containing 0.1 % TFA

Flow Rate 0.8 ml/min

Gradient 0.20 min 5% B; 5% to95% B in 7.80 min, 1.00 min 95% B

Method 10minLC_v003

Column Waters BEH C18 50x2.1 mm, 1.7 μίτι

Column Temperature 50 °C

Eluents A: H20, B: acetonitrile, both containing 0.1 % TFA

Flow Rate 0.8 ml/min

Gradient 0.20 min 5% B; 5% to95% B in 7.80 min, 1.00 min 95% B

Method 2minLC_v001

Column Waters BEH C18 100x2.1 mm, 1.7 μίτι

Column Temp. 50 °C

Eluents A: H20, B: acetonitrile, both containing 0.1 % TFA

Flow Rate 0.7 ml/min

Gradient 0.25 min 5% B; 5% to95% B in 1.00 min, 0.25 min 95% B

Method 2minLC_v002

Column Waters BEH C18 50x2.1 mm, 1.7 μηι

Column Temperature 50 °C

Eluents A: H20, B: methanol, both containing 0.1 % TFA

Flow Rate 0.8 ml/min

Gradient 0.20 min 5% B; 5% to95% B in 1.30 min, 0.25 min 95% B

Method 2minLC_v003

Column Waters BEH C18 50x2.1 mm, 1.7 μίτι

Column Temperature 50 °C

Eluents A: H20, B: acetonitrile, both containing 0.1 % TFA

Flow Rate 0.8 ml/min

Gradient 0.20 min 5% B; 5% to95% B in 1.30 min, 0.25 min 95% B

Method 10minC18

Column: Gemini C18 100x3 mm, 3 micron (continued)

Method 10minC18

Column Temperature 50 °C

Eluents: A: H20, B: Methanol, 0.1 % formic acid

Flow rate: 1 ml/min

Gradient: 0.00 min 0% B, 10.00 min 95% B

Method AD25IPA_DEA

Mobile Phase: 25% isopropanol + 0.1 %v/v DEA/75 % C02

Column: Chiralpak AD-H, 250 x 10 mm id, 5 μίτι

Detection: UV @ 220nm

Flowrate: 10 ml/min [0139] Example compounds of the present invention include Preparation of Final Compounds

Example 1.0 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide [0140]

[0141] 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylicacid (Intermediate A) (397 mg, 1.392 mmol), 3-amino-1,1,1-trifluoro-2-methyl-propan-2-ol hydrochloride (250 mg, 1.392 mmol) and FIATU (529 mg, 1.392 mmol) were dissolved in DMF (10 ml) and stirred at RT for 2 min. 4-Methylmorpholine (0.413 ml, 4.18 mmol) was added and stirring continued at RT for 3 h. The reaction mixture was poured onto ice/water (100 ml ) and extracted with EtOAc (250 ml). The organic extract was washed with sat NH4CI solution (~50 ml), dried over MgS04 and concentrated in vacuo to give a pale brown oil. The oil was dissolved in CFiCI3 (~3 ml) and loaded onto a 24g ISCO (silica) column eluting with iso-hexane:EtOAc to afford the title product; LC-MS Rt = 1.46 mins; [M+H]+ 410.1, Method 2minLC_v002.1H NMR (400 MHz, DMSO-d6) δ 8.30 (NH, t), 7.72 (1H, s), 7.29 (NH2, b s), 6.28 (OH, s), 3.68 (1 H, dd), 3.47 (1 H, dd), 1.24 (3H, s). 19F NMR (400 MHz, DMSO-d6) δ -62.71 (CF3, s),-80.48 (CF3, s).

[0142] The compounds of the following tabulated Examples (Table 2) were prepared by a similar method to that of Example 1 from the appropriate starting compound and amine. Single enantiomers were prepared by using chiral amines or by separation of the product by Supercritical Fluid Chromatography. The preparations of the starting compounds and amines are described in the Intermediates section, unless they are commercially available. DIPEA or TEA may have been used in place of 4-methylmorpholine in some reactions.

Table 2

(continued)

(continued)

(continued)

(continued)

(continued)

(continued)

(continued)

(continued)

(continued)

Example 2 and 3 [0143] These compounds namely, 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide. (Ex. 2)

and 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide. (Ex.3)

are prepared by chiral separation of 3-amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hy-droxy-2-methyl-propyl)-amide. (Example 1) using Supercritical Fluid Chromatography under the following conditions:

Mobile Phase : 12% isopropanol + 0.1 % DEA/88% C02

Column: Chiralpak OJ-H, 250 x 10 mm id, 5 μίτι

Detection: UV @ 220nm

Flowrate: 10 ml/min

Sample concentration: 347mg in 5 ml EtOFI.

Injection volume: 50μΙ [0144] Example 2: First eluted peak: 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylicacid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide.

[0145] LC-MS: Rt = 4.97min [M+H]+ 410.1/412.2 (Method 10minLC_v002). 1H NMR (400 MHz, DMSO-d6) δ 8.30 (NH, t), 7.72 (1 H, s), 7.29 (NH2, b s), 6.28 (OH, s), 3.68 (1 H, dd), 3.47 (1 H, dd), 1.24 (3H, s) 19F NMR (400 MHz, DMSO-d6) d -62.70 (CF3, s), -80.47 (CF3, s)

Optical rotation [a]21D at 589 nm +14.4°(c= 0.522, MeOH).

Example 3: Second eluted peak: 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hy-droxy-2-methyl-propyl)-amide. LC-MS Rt = 4.94 min [M+H]+ 412.1 (Method 10minLC_v002). 1H NMR (400 MHz, DMSO-d6) δ 8.30 (NH, t), 7.72 (1 H, s), 7.29 (NH2, b s), 6.28 (OH, s), 3.68 (1 H, dd), 3.47 (1 H, dd), 1.24 (3H, s) 19F NMR (400 MHz, DMSO-d6) d -62.70 (CF3, s), -80.48 (CF3, s).

The stereochemistry of this compound was confirmed by X-ray crystallography.

Example 4, 5 and 6 [0146] This compound namely, 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide. (Ex. 4),

was prepared according to the following procedure: A solution comprising 3-amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (Intermediate D)(4 g, 16.94 mmol) and 3-amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride (Intermediate R) (3.04 g, 16.94 mmol) in NMP (188 ml) was treated with HATU (7.73 g, 20.33 mmol) followed by dropwise addition (2 ml portions) of DIPEA (8.88 ml, 50.8 mmol) over 1 hour. After stirring for a further hour, the reaction mixture was poured into water (450 ml) and EtOAc (450 ml). The aqueous phase was acidified with 5M HCI (50 ml) and the layers were separated. The organic portion was washed with 2M NaOH (200 ml), water (4 x 200 ml), brine (2 x 100 ml), dried over MgS04, filtered and concentrated in vacuo to afford a brown solid. Purification of the solid by chromatography on silica (220 g pre-packed silica cartridge) eluting with 0-50 % EtOAc in iso-hexane afforded the racemate, 3-amino-6-methoxy-5-trifluorome-thyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide (Ex. 4)as a yellow solid; 1 H NMR (400 MHz, DMSO-d6)6 8.3(1 H, t), 7.7(1 H, s), 6.7(2H,s), 6.2(1 H, s), 3.9(3H,s),3.7(1 H,m), 3.5(1 H, m), 1.2(3H,s). LC-MS: Rt 1.24 min; MS m/z 362.4 [M+H]+; Method 2minLC_v003.

[0147] Chiral separation of the racemate by Supercritical Fluid Chromatography was carried out using the following conditions to afford the compounds listed hereinafter:

Mobile Phase: 12% 2-propanol + 0.1% DEA/50% C02

Column: Chiralcel OD-H, 250x 10 mm id, 5 μίτι (2 columns linked in series)

Detection: UV @ 220nm

Flowrate: 10ml/min

Sample concentration: 3.5 g in 30 ml EtOH Injection volume: 10Ομ,Ι

Examples 5 and 6 are enantiomers.

[0148] Example 5: First eluted peak Rt = 7.30 minutes. 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide:

1 H NMR (400MHz, DMSO - d6) δ 8.3 (1 H, t), 7.6 (1 H, s), 6.6 (2H, broad), 6.2 (1 H, s), 3.9 (3H, s), 3.6 (1 H, m), 3.5 (1 H, m), 1.3 (3H, s); LC-MS Rt = 1.15 mins, [M+H]+ 362.4 (Method 2minLC_v003).

Optical rotation [a]21D at 589 nm -20.83°(c= 0.513, MeOH).

The stereochemistry of this compound was confirmed by X-ray crystallography.

[0149] Example 6: Second eluted peak Rt = 8.29 minutes. 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide

1 H NMR (400MHz, DMSO-d6) δ 8.3 (1H, t), 7.6 (1 H, s), 6.6 (2H, broad), 6.2 (1 H, s), 3.9 (3H, s), 3.6 (1 H, m), 3.5 (1 H, m), 1.3 (3H, s); LC-MS Rt = 1.15 mins [M+H]+ 362.4 (Method 2minLC_v003).

[0150] Alternatively, Example 5 may be prepared according to the following method:

To a solution of 3-amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (Intermediate D) (10 g, 42.3 mmol) and (S)-3-amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride (Intermediate RA)(7.60 g, 42.3 mmol) in NMP (400 ml) was added HATU (19.3 g, 50.8 mmol) followed by dropwise addition of DIPEA (22.19 ml, 127 mmol) over ~1 hr. After stirring at room temperature for 30 min, the mixture was added to EtOAc (2 L), washed with 1 M NaOH (2 X 1 L), water (1 L), brine (1 L), dried (MgS04) and evaporated under reduced pressure to give the crude product as a dark brown oil.

[0151] Purification by chromatography on silica eluting with a gradient of 1 to -25% of EtOAc in iso-hexane afforded a yellow oil. Recrystallisation of the oil from iso-hexane/DCM afforded 3-amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide as a crystalline solid; 1H NMR (400MHz, DMSO - d6) δ 8.28 (1 H, t), 7.66 (1 H, s), 6.67 (2H, s), 6.27 (1 H, s), 3.91 (3H, s), 3.65 (1 H, m), 3.45 (1 H, m), 1.24 (3H, s). 19F NMR (376MHz, DMSO - d6) -62.58 ppm (s), -80.43 ppm (s)

Example 7 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide.

[0152]

[0153] A mixture comprising 3-amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hy-droxy-2-methyl-propyl)-amide (Ex. 3)(100 mg, 0.244 mmol), 4-fluorophenylboronic acid (37.5 mg, 0.268 mmol) and 1,1’bis(diphenylphosphoshio) ferrocenepalladium dichloride (19.90 mg, 0.024 mmol) was suspended in THF (2 ml) and 1 M Cs2C03 (0.667 ml). The vial was flushed with N2, sealed and heated at 160°C using microwave radiation for 15 minutes. The mixture was partitioned between EtOAc (50ml) and water (50ml). The organic portion was separated and washed with brine (30ml), dried (MgS04), filtered through Celite® (filter material) and concentrated in vacuo. The crude residue was dissolved in DMSO (2ml) and purified by mass directed LCMS using MeCN/Water/0.1% TFA eluent to afford clean product. The product fraction obtained as MeCN/Water/0.1% TFA solution was poured into EtOAc (50 ml) and washed with saturated NaHC03 (50 ml) to free base the product. The organic portion were combined, dried (MgS04) and concentrated in vacuo to afford the title compound as a pale orange crystalline solid; 1 H NMR (400MHz, DMSO -d6) δ 8.4 (1 H, m), 7.7(1 H, s), 7.49 (2H, m), 7.29 (2H, t), 7.2 (2H, brs), 6.22(1 H, s), 3.68 (1 H, m), 3.44(1 H, m), 1.22 (3H, s); LC-MS Rt 4.41 mins [M+H]+ 426 (Method 10minLC_v003).

Example 8 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide.

[0154]

[0155] This compound was prepared from 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide (Ex. 2) analogously to Example 8. 1 H NMR (400MHz, DMSO - d6) δ 8.42 (1 H, m), 7.7 (1 H, s), 7.5 (2H, m), 7.3 (2H, t), 7.21 (2H, brs), 6.24 (1 H, s), 3.68 (1 H, m), 3.44 (1 H, m), 1.22 (3H, s); LC-MS Rt = 4.39 mins [M+H]+ 426 (Method 10minLC_v003).

Example 9 and 10 [0156] The enantiomers of 3-amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trif-luoro-2-hydroxy-2-methyl-propyl)-amide were prepared from 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyrid-ine-2-carboxylic acid (Intermediate H) and 3-amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride analogously to Example 1 and separated by chiral separation using Supercritical Fluid Chromatography:

Example 9: First eluted peak. Entantiomerl of 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carbox-ylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide:

1 H NMR (400 MHz, DMSO-d6) δ 8.38 (t,1 H), 7.83 (s,1 H), 7.78 (s,1 H), 7.60 (d,1 H), 7.54 (d,1 H), 7.39 (br s ,2H), 6.25 (brs, 1 H). 3.71 (dd,1 H),3.48(dd,1 H), 1.26 (s,3H); LC-MS Rt = 1.65 mins [M+H]+476 (Method 2minLC_v002).

[0157] Example 10: Second Eluted peak. Enantiomer2 of 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine- 2- carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide.

1 H NMR (400 MHz, DMSO-d6) δ 8.38 (t,1 H), 7.83 (s,1 H), 7.78 (s,1 H), 7.60 (d, 1 H), 7.54 (d,1 H), 7.39 (brs ,2H), 6.25 (brs, 1 H). 3.71 (dd,1 H), 3.48 (dd,1 H), 1.26 (s,3H); LC-MS Rt 1.65 mins [M+H]+=476.1 (Method 2minLC_v002).

Example 11 3- Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide [0158]

[0159] To a stirred suspension of 3-amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide (Ex. 1.10) (180mg, 0.505mmol) and 4-fluorophenylboronic acid (106mg, 0.758mmol) in a 2:1 mixture of toluene:EtOH (12ml) under nitrogen was added 2M Na2C03(aq) (1.011 ml, 2.022 mmol) followed by Pd(dppf)CI2 .CH2CI2 adduct (41 mg, 0.051 mmol). The reaction mixture was heated using microwave radiation at 140°C for 1 hour and then allowed to cool to RT. The mixture was diluted with EtOAc (100ml) and washed with water (100ml). The organic phase was separated, filtered through Celite® (filter material) dried (MgS04) and concentrated in vacuo to yield a brown oil/solid. Purification by chromatography on silica eluting with MeOH/DCM yielded a yellow oil/solid. This was passed through a 500mg Isolute® Si-TMT cartridge (2,4,6-trimercaptotriazine silica, pre-wetted with DCM) eluting with 30% MeOH/DCM (50 ml) to afford a yellow oil/solid. The crude product was dried in vacuo and slurried in ~0.5ml DCM. The resulting suspension was removed by filtration and the filtrate was evaporated to yield the title compound as a light yellow/brown foam-like solid; LC-MS Rt = 5.30 mins [M+H]+ 372 (Method 10minLC_v002). 1 H NMR (400 MHz, DMSO-d6), δ 8.29 (1 H, t), 7.69 (1 H, s), 7.49 (2H, t), 7.29 (2H, t), 7.22 (2H, s), 4.63 (1 H, s), 3.24 (2H, d), 1.08 (6H, s).

Example 12 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide [0160]

Step 1: 3-(2,5-Dimethyl-pyrrol-1-yl)-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide [0161] This compound was prepared from 3-(2,5-Dimethyl-pyrrol-1 -yl)-6-methoxy-5-trifluoromethyl-pyridine-2-carbox-ylic acid (Intermediate D2) and 3-amino-1,1,1-trifluoropropan-2-ol analogously to Example 1; LC-MS Rt = 1.50mins [M+H]+ 426 (Method 2minLC_v002).

Step 2: 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide [0162] 3-(2,5-Dimethyl-pyrrol-1-yl)-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide (350 mg, 0.823 mmol) was dissolved in EtOH (14 ml) and water (7 ml). Hyroxylamine hydrochloride (572 mg, 8.23 mmol) was added followed by TEA (167 mg, 1.646 mmol) and the mixture was heated at reflux overnight. After cooling the RT, the mixture was purified by reverse phase chromatography eluting with MeOH; water (0.1 % TFA) to afford the title compound as a pale yellow solid; LC-MS Rt= 4.20 min [M+H]+ 348.2 (Method 10minLC_v002) 1H NMR (400 MHz, DMSO-d6) δ 8.47 (NH, t), 7.66 (1H, s), 6.68 (NH2, bs), 6.51 (OH, d), 4.27-4.20 (1 H, m), 3.93 (3H, s), 3.64-3.58 (1 H, m), 3.44-3.37 (1 H, m) 19F NMR (400 MHz, DMSO-d6) d -62.67 (CF3, s), -77.05 (CF3, s), Trace TFA.

Example 14 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-trifluorome-thyl-propyl)-amide [0163]

[0164] This compound was prepared from 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trif-luoro-2-hydroxy-2-trifluoromethyl-propyl)-amide (Ex. 1.28) and 2-methylpyridine-5-boronic acid analogously to Example 8. LC-MS Rt 1.28 min; 477[M+H]+; (Method 2minLC_v002); 1H NMR (400 MHz, MeOD) δ 8.50 (1H, s), 7.85 (1 H, dd), 7.69 (1 H, s), 7.40 (1 H, d), 4.00 (2H, s), 2.62 (3H, s).

Example 15 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide [0165]

[0166] This compound was prepared by chiral separation of 5-amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide (Example 1.29) using Supercritical Fluid Chromatography; LC-MS Rt 3.15 min [M+H]+423; (Method 10minLC_v002); 1H NMR (400 MHz, DMSO-d6) δ 8.53 (1 H, s), 8.49 (1 H, t), 7.75 (1 H, d), 7.71 (1 H, s), 7.35 (1 H, d), 7.25 (2H, s), 6.22 (1 H, s), 3.69 (1H, dd), 3.42 (1H, dd), 2.54 (3H, s), 1.22 (3H, s). SFC Retention Time: 4.87 min.

Example 16 and 17 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide and 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide [0167]

Step 1: 3-(2,5-Dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide [0168] To a stirred solution of 3-(2,5-dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid (Intermediate M) (1.16 g, 3.29 mmol) in NMP (32 ml) was added 3-Amino-1,1,1-trifluoro-2-methyl-propan-2-ol hydrochloride (commercially available) (591 mg, 3.29 mmol) followed by HATU (1.25 g, 3.29 mmol) and NEt3 (918 ul, 6.59 mmol) and the reaction mixture was left to stir at RT. After 1 h a further 0.2 equiv. NEt3 was added. After 15 min a further 0.4 equiv. NEt3 and 0.2 equiv. amine were added. After 30 min a further 0.1 equiv HATU was added. After 30 min most of the starting material had been consumed. The reaction mixture was added to EtOAc (50 ml), washed with 0.1 M NaOH and the aqueous layer was back extracted with EtOAc (2 x 50 ml). The combined organic extracts were washed with water (2 X 150 ml), brine (100 ml), dried (MgS04) and concentrated in vacuo to give the crude product as an orange oil.

[0169] The crude material was purified by chromatography on silica eluting with 0-15% EtOAc in iso-hexane to afford the title product as a yellow solid; LC-MS Rt 1.32 min; MS m/z 478.2 [M+H]+; Method 2minLC_v003.

Step 2: 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide [0170] To a stirred solution of 3-(2,5-dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trif-luoro-2-hydroxy-2-methyl-propyl)-amide (985 mg, 2.064 mmol) in 2:1 EtOH/H20 (7.5 ml) was added hydroxylamine hydrochloride (1.43 g, 20.64 mmol) followed by NEt3 (575 ml, 4.13 mmol). The reaction mixture was heated to reflux (~98 °C) for 11.5 hours and then allowed to cool to RT. The solvent was removed in vacuo and the resulting residue was partitioned between EtOAc (25 ml) and water (25 ml). The aqueous layer was separated and extracted with EtOAc (2 X 25 ml) and the combined organic extracts were washed with brine (50 ml), dried (MgS04) and concentrated in vacuo. The crude material was purified by chromatography on silica eluting with 0-25% EtOAc in iso-hexane to afford the title product as a pale yellow solid; LC-MS: Rt 1.24 min; MS m/z 400.0 [M+H]+; Method 2minLC_v003.

Step 3: 3-Amino-5.6-bis-trifluoromethvl-pyridine-2-carboxvlic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide and 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide [0171]

[0172] These compounds were prepared by chiral separation of 3-amino-5,6-bis-trifluoromethylpyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; Enantiomer 1 : LC-MS Rt 1.23 min; MS m/z400.0 [M+H]+; Method 2minLC_v003. SFC Retention Time 5.07 min.

[0173] Enantiomer2: LC-MS Rt 1.23 min; MS m/z400.0 [M+H]+; Method 2minLC_v003. SFC Retention Time 5.13 min. Example 18 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methylpropyl)-5-(trifluoromethyl)picolina- mide [0174]

[0175] The title compound was prepared analogously to Example 1 from3-amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylicacid (Intermediate D) and 3,3,3-trifluoro-N2-(4-methoxybenzyl)-2-methylpropane-1,2-diamine (Intermediate N). DIPEA was used in this reaction. 1 H NMR (400 MHz, DMSO-d6) δ 8.27 (1 H, m), 7.68 (1 H, s), 7.25 (2H, d), 6.83 (2H, d), 6.70 (2H, s), 3.85 (3H, s), 3.75 (2H, m), 3.72 (3H, s), 3.70 (1 H, m), 3.47 (1 H, m), 2.80 (1 H, t), 1.24 (3H, s)

Example 19 3-Amino-N-(2-amino-3,3,3-trifluoro-2-methylpropyl)-6-methoxy-5-(trifluoromethyl) picolinamide [0176]

[0177] A mixture comprising 3-amino-6-methoxy-N-(3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methylpropyl)-5-(tri-fluoromethyl)picolinamide (Ex. 18) (0.9 g, 1.873 mmo)in TFA (50 ml) was heated to 50°C for 2h. After cooling to RT, the pH was adjusted to pH 12 using 2M NaOH. The product was extracted with DCM and the organic extract was washed with water, dried over MgS04 and concentrated in vacuo. The crude product was loaded onto a SCX-2 cartridge eluting with MeOH followed by 2M NH3 in MeOH. The methanolic ammonia fractions were concentrated in vacuo and dried under vacuum to afford the title compound. 1 H NMR (400 MHz, DMSO-d6) δ 8.35 (1 H, m), 7.67 (1 H, s), 6.67 (2H, s), 3.93 (3H, s), 3.58 (1 H, m), 3.40 (1 H, m), 2.22 (2H, s), 1.14 (3H, s). LC-MS Rt 0.94 min; MS m/z 361.2 [M+H]+; Method 2minLC_v003.

Example 20 3-Amino-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide [0178]

Step 1: 3-(2,5-Dimethyl-1 H-pyrrol-1-yl)-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluorome-thyl)picolinamide [0179] The title compound was prepared from Intermediate DA analogously to Example 1; LC-MS Rt 1.42 min; MS m/z 479.3 [M+H]+; Method 2minLC_v003.

Step 2: 3-Amino-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide [0180] This compound was prepared from 3-(2,5-dimethyl-1 H-pyrrol-1-yl)-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hy-droxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide analogously to Intermediate D(final step). The resulting racemate was separated by SFC to afford the title compound; First eluted peak: 1 H NMR (400MHz, DMSO - d6) δ 8.24 (1 H, m), 7.6 (1 H, s), 6.4 (2H, brs), 6.32 (1 H, s), 3.64 (1 H, m), 3.48 (1 H, m), 3.35 (4H), 1.88 (4H, m), 1.25 (3H, s); LC-MS Rt 3.87 min; MS m/z 401.3 [M+H]+; Method 10minLC_v003.

Example 21 (S)-3-amino-6-ethoxy-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoro methyl)picolinamide [0181]

[0182] The title compound was prepared from Intermediate DB and Intermediate R analogously to Example 20; 1 H NMR (400 MHz, DMSO-d6) δ 8.3 (1 H, t), 7.7 (1 H, s), 6.6 (2H, broad), 6.3 (1 H, s), 4.4 (2H, q), 3.6 (1 H, mult), 3.5 (1 H, mult), 1.3 (3H, t), 1.2 (3H, s). LC-MS Rt 1.20 min; MS m/z 376.2 [M+H]+; Method 2minLC_v003.

Example 22 3-Amino-6-bromo-N-(2-morpholinoethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide [0183]

[0184] To a stirred solution of 3-amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (Intermediate C) (250 mg, 0.874 mmol) in NMP (8 ml) was added 4-(2-aminoethyl)morphonline (138 ul, 1.049 mmol) followed by DIPEA (763 ul, 4.37 mmol). To this solution was then added HATU (499 mg, 1.311 mmol) in portions and the reaction mixture was left to stir at RT for 1 hour. A further 1 equiv. of 4-(2-aminoethyl)morphonline was added. After a further 1.5 hr, 0.5 equiv. HATU (166 mg,0.425 mmol) was added and the RM was left to stir for a further 30 min. The mixture was added to EtOAc (50 ml) and washed with 0.1 M NaOH (50 ml). The aqueous layer was back extracted with EtOAc (50 ml). The combined organics were washed with water (50 ml), brine (50 ml), dried over magnesium sulfate and evaporated under reduced pressure to give a brown oil (418 mg). The crude product was purified by chromatography (Biotage- silica 20 g/ 70 ml column, 3:1 EtOAc/iso-hexane). The resulting yellow residue was loaded onto an SCX-2 cartridge (10 g) that had been pre-wetted with MeOH. The cartridge was washed with MeOH (140 ml) and eluted with 3.5M ammonia in methanol solution (70 ml). The appropriate fractions were evaporated under reduced pressure to give a solid. This solid was dissolved in EtOAc and filtered under vacuum. The filtrate was evaporated under reduced pressure and then dried in vacuo to afford the title compound as a yellow solid; LC-MS: Rt 2.61 min; MS m/z 398.2 [M+H]+; Method 10minLC_v002 1H NMR (400 MHz, DMSO-d6) δ 8.70 (1H, s), 8.10 (2H, s), 3.58 (4H. t), 3.40 (2H, q), 2.45 (2H, m), 2.40 (4H, s).

Example 23 N-(2-(1H-imidazol-2-yl)propyl)-3-amino-6-bromo-5-(trifluoromethyl)pyrazine-2-carboxamide [0185]

[0186] The title compound was prepared from 3-amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (Intermediate C) and 2-(1 H-imidazol-2-yl)propan-1 -amine (prepared according to the procedure of Steffens, Robert; Schunack, Walter. Histamine analogs, XXVI. Racemic histamine H1-agonists. Archiv der Pharmazie (Weinheim, Germany) (1984), 317(9), 771-6; 1 H NMR (400 MHz, DMSO-d6) δ 11.8(1 H, s), 9.0(1 H, t), 8.1 (2H, s), 7.0(1 H, s), 6.8(1 H, s), 3.55 (2H, m), 3.15 (1 H, m), 1.2 (3H, d). LC-MS [M+H]+ 393.0/395.1

Example 24a and 24b

Enantiomers of 3-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5,6-bis(trifluoromethyl) pyrazine-2-car-boxamide [0187]

[0188] The title compound was prepared from Intermediate BA and 3-amino-1,1,1-trifluoro-2-methylpropan-2-ol analogously to Example 4. Chiral separation of the racemate by Supercritical Fluid Chromatography afforded the title compound;

Example 24a: First eluted peak: Enantiomer 1 of 3-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5,6-bis(trifluor-omethyl) pyrazine-2-carboxamide; 1 H NMR (400MHz, DMSO - d6) δ 8.61 - 8.74 (1 H, broad hump), 8.5 - 8.61 (1 H, broad hump), 8.46 (1 H, t), 6.3 (1 H, s), 3.69 (1 H, m), 3.5 (1 H, m), 1.29 (3H, s) LC-MS: Rt 4.23 min; MS m/z 401.2 [M+H]+; Method 10minLC_v003.

Example 24b: Second eluted peak: Enantiomer 2 of 3-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5,6-bis(trif-luoromethyl) pyrazine-2-carboxamide; 1 H NMR (400MHz, DMSO - d6) δ 8.61 - 8.76 (1 H, broad hump), 8.5 - 8.60 (1 H, broad hump), 8.46 (1 H, t), 6.3 (1 H, s), 3.69 (1 H, m), 3.5 (1 H, m), 1.29 (3H, s) LC-MS: Rt 4.24 min; MS m/z 401.2 [M+H]+; Method 10minLC_v003.

Optical rotation [a]21D at 589 nm +22.0°(c= 0.517, MeOH).

Example 25 3-Amino-6-(1 -methyl-1 H-pyrazol-4-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolina-mide [0189]

Step 1: 3-Amino-6-(1-methyl-1 H-pyrazol-4-yl)-5-(trifluoromethyl)picolinic acid [0190] 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A4) (500 mg, 1.672 mmol), PdCl2(dppf).CH2CI2 adduct (205 mg, 0.251 mmol), 1-methyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1 H-pyrazole (383 mg, 1.839 mmol) and Cs2C03 (6.69 ml, 6.69 mmol) in THF (12 ml) under N2, was heated using microwave radiation at 150°C for 10 minutes. 2M NaOH (5 ml) was added and the mixture was stirred at RT overnight. The mixture was filtered through Celite® (filter material) and the organic solvent was removed. The resulting aqueous layer was washed with EtOAc and acidified to pH 1. The product was extracted with DCM and concentrated in vacuo to afford the title compound;

Step 2; 3-Amino-6-(1-methyl-1 H-pyrazol-4-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolina-mide [0191] The title compound was prepared from 3-amino-6-(1 -methyl-1 H-pyrazol-4-yl)-5-(trifluoro methyl)picolinic acid and 3-amino-1,1,1-trifluoro-2-methylpropan-2-ol analogously to Example 4 1 H NMR (400 MHz, Methanol-d4) δ 7.97 (1 H, s), 7.85 (1 H, s), 7.60 (1 H, s), 3.97 (3H, s), 3.77 (1 H, m), 3.56 (1 H, m), 1.37 (3H, s) LC-MS: Rt 3.22 min; MS m/z 412.3 [M+H]+; Method 10minLC_v003.

Example 26 3-Amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(2-methoxyphenyl)-ethyl]-amide [0192]

[0193] The title compound was prepared from 3-amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid (Intermediate PA) and the appropriate amine; MS m/z 406.93[M+H]+

Preparation of Intermediates

Intermediate A 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [0194]

Intermediate A1 : 2-Bromo-3-nitro-5-trifluoromethyl-pyridine [0195] 3-Nitro-5-(trifluoromethyl)pyridin-2-ol (31.00 g, 149 mmol) was dissolved in acetonitrile (250 ml) to give a dark brown solution. Phosphorus(V) oxybromide (85 g, 298 mmol) was added and the mixture was heated at reflux for 4.5 hours and then stirred at RT overnight. The reaction mixture was quenched by pouring into vigorously stirring water (600 ml) containing sodium hydrogencarbonate (110g). The dark brown mixture was extracted with DCM (3 x 200 ml) and the organic phase was washed with water (200ml) and brine (100ml), dried (MgS04) and concentrated in vacuo to afford the title product as a brown oil. 1H-NMR: [400MHz, CDCI3, δΗ 8.87 (1 H, d, J = 1,4Hz, ArH), 8.39 (1 H, d, J = 1,9Hz, ArH).

Intermediate A2: 3-Nitro-5-trifluoromethyl-pyridine-2-carbonitrile [0196] 2-Bromo-3-nitro-5-trifluoromethyl-pyridine (10.00 g, 36.87 mmol) was dissolved in toluene (250 ml) with stirring to give a pale yellow solution. Tetrabutylammonium bromide (11.90 g, 36.9 mmol) was added followed by copper(l) cyanide (9.92 g, 111 mmol) and the mixture was heated at reflux for 10 h. After cooling to RT, the reaction mixture was partitioned between water (750 ml) and EtOAc (750ml). The organic fractions were combined, washed with water (2 x 250ml) and brine (100ml), dried (MgS04) and concentrated in vacuo to afford the title product. 1H-NMR: [400MHz, DMSO-d6 δΗ 9.55 (1 H, m, ArH), 9.24 (1 H, m, ArH)

Intermediate A3: 3-Amino-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester [0197] 3-Nitro-5-trifluoromethyl-pyridine-2-carbonitrile (6.5 g, 29.9 mmol) was dissolved in EtOAc (150 ml) to give a pale yellow solution and placed under an atmosphere of nitrogen. 10 % Palladium on activated carbon (3.19 g, 2.99 mmol) was added and the reaction mixture stirred under an atmosphere of hydrogen for 18 hours. The reaction mixture was filtered and concentrated in vacuo. The crude residue was dissolved in HCI cone. (45 ml) and heated to reflux for 24 hours. The reaction mixture was allowed to cool to RT and concentrated in vacuo. The solid was dissolved in MeOH (300 ml) and sulfuric acid (14.4 ml) was added. The resulting solution was heated at reflux for 48 hours. The reaction was allowed to cool to RT, then neutralised by addition of 10% NaHC03(aqj (600 ml). The product was extracted into DCM (3 x 200 ml) and the combined organic phases were washed with water (200ml), brine (50 ml), (MgS04) and concentrated in vacuo. The resulting solid was purified by chromatography on silica : Eluant gradient: isohexane (500 ml), 10% EtOAc in isohexane (1000 ml), 20% EtOAc in isohexane (1500 ml) to afford the titled compound as a pale yellow solid 1H-NMR: [400MHz, DMSO-d6, δΗ 8.13 (1H, d, J = 1.7Hz, ArH), 7.60 (1H, d, J = 1.3Hz, ArH), 7.01 (2H, br, NH2), 3.85 (3H, s, ArOCH3), m/z 221.1 [M+H]+

Intermediate A4: 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester [0198] 3-Amino-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (9.49 g, 43.16 mmol) was suspended in water (300 ml). Sulfuric acid (4.60 ml, 86 mmol) was added followed by dropwise addition over 30 minutes of a solution of bromine (2.222 ml, 43.1 mmol) in acetic acid (29.6 ml, 517 mmol). The reaction mixture was stirred at RT for 18 hours. A further 100ml of waterwas added, followed by a further 0.25 equivalents of the bromine/AcOH mixture (550 μι. bromine in 7.4ml AcOH) and the reaction mixture stirred at RT for an additional 90 minutes. The reaction mixture was diluted with 500ml water and neutralised by addition of solid NaHC03 (~85 g). The suspension was extracted with DCM (3 x 300 ml) and the combined organic phases washed with sat.NaHC03(aq) (250 ml), water (250 ml) and brine (100 ml), dried (MgS04) and concentrated in vacuo. The crude material was recrystallised from boiling MeOH (-300 ml) to give the title product as a pale orange solid m/z 301.0 [M+H]+ 1H-NMR: [400MHz, DMSO-d6 δΗ 7.77 (1 H, s, ArH), 7.17 (2H, s, NH2), 3.86 (3H, s, ArC02CH3).

Intermediate A: 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [0199] 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester(1.40 g, 4.68 mmol) was suspended in MeOH (15 ml); Sodium hydroxide (2.0 M aqueous solution) (14.04 ml, 28.1 mmol) was added and the suspension was stirred at RT overnight. The mixture was concentrated in vacuo and the resulting residue was dissolved in water (100 ml) and then acidifed by the addition of 5.0M HCI(aq). The product was extracted into ethyl acetate (2 x 75 ml) and the combined organic extracts were washed with water (50 ml), brine (25 ml), dried (MgS04) and concentrated in vacuo to afford the title product as a yellow solid. 1H-NMR: [400MHz, DMSO-d6, δΗ 13.24 (1 H, br s, C02H), 7.74 (1 H, s, ArH), 7.17 92H, brs ArNH2). m/z 285.1,287.1 [M+H]+

Intermediate B 3-Amino-5-trifluoromethyl-pyrazine-2-carboxylic acid ethyl ester [0200]

[0201] Intermediate B1 : Carbamimidoyl-nitroso-acetic acid ethyl ester To a solution of 2M ammonia in Ethanol (152 ml, 0.304 mmol) at 0°C to 5°C, ethyl ethoxycarbonylacetimidate HCI (25 g, 0.127 mmol) was added over 30 minutes. The reaction was stirred vigorously at this temperature for 3 hours, after which a solution of sodium nitrite in water (9.63 g, 0.139 mmol) was added in a single portion. The pH of the mixture was adjusted to pH6 with the addition of 5N HCI. The reaction mixture was left to stir at RT overnight. The yellow precipitate formed was filtered under vacuum, washed with water and dried to give the title compound; 1H NMR (400 MHz, DMSO - d6) δ 10.1 (2H, br s), 7.6 (2H, br s), 4.3 (2H, q), 1.3 (3H, t).

[0202] Intermediate B2: Amino-carbamimidoyl-acetic acid ethyl ester To a solution of carbamimidoyl-nitroso-acetic acid ethyl ester (5.5 g, 31.4 mmol) in ethanol/5M HCI (1:1 ratio, 250 ml) was added 10% Pd/C (1.3 g). The reaction mixture was hydrogenated (Η2^) at low pressure over 2 nights. The Pd/C was filtered through Celite®(filter material) and the filtrate reduced in vacuo to give the title compound as a white solid. This was taken through to the next step as crude.

[0203] Intermediate B: 3-Amino-5-trifluoromethyl-pyrazine-2-carboxylic acid ethyl ester To a mixture of amino-car-bamimidoyl-aceticacid ethyl ester (2 g, 9.22 mmol) and water (50 ml), a 20% aqueous solution of trifluoropyruvic aldehyde (2.32 g, 18.43 mmol) was added. To this mixture, sodium acetate (5.29 g, 64.52 mmol) was added (pH of the reaction mixture was pH5). The reaction mixture was left to stir at RT overnight. The resultant precipitate was filtered under vacuum purification by chromatography on silica eluting with iso-hexane: EtOAc (gradient of 0 to 10 % EtOAc) afforded the title compound 1H NMR (400 MHz, DMSO - d6) δ 8.4 (1 H, s), 7.8 (2H, brs), 4.4 (2H, q), 1.4 (3H, t).

Intermediate BA 3-Amino-5,6-bis(trifluoromethyl)pyrazine-2-carboxylic acid [0204]

Step 1 : Ethyl 3-amino-5,6-bis(trifluoromethyl)pyrazine-2-carboxylate [0205] The title compound was prepared from amino-carbamimidoyl-acetic acid ethyl ester (Intermediate B2) and 1,1,1,4,4,4-hexafluorobutane-2,3-dione analogously to Intermediate B; 10 LCMS Rt = 4.72 minutes, [M+H]+ 304.2/326.1 Method 10minLC_v002.

Step 2: 3-Amino-5,6-bis(trifluoromethyl)pyrazine-2-carboxylic acid [0206] To a stirring solution of ethyl 3-amino-5,6-bis(trifluoromethyl)pyrazine-2-carboxylate (300 mg, 0.990 mmol) in EtOH (10 ml), 2M NaOH (0.495 ml, 0.990 mmol) was added dropwise over 1 minute. After stirring at RT for 30 minutes the reaction mixture was poured into water (30 ml) and the pH was adjusted to pH 4 by addition of 1 M HCI. The mixture was extracted with EtOAc (2 x 50ml) and the combined organic extracts were washed with brine (30ml), dried over MgS04 (5g), filtered and concentrated in vacuo to afford the title compound as an off white crystalline solid; 1H NMR (400 MHz, DMSO - d6) δ 8.6-9.2 (2H, broad hump), 7.8-8.3 (2H, broad hump), 4.4 (2H, q), 1.32 (3H, t).

Intermediate C 3-Amino-6-bromo-5-trifluoromethvl-pyrazine-2-carboxvlic acid [0207]

Intermediate C1: 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid ethyl ester [0208] To a solution of 3-amino-5-trifluoromethyl-pyrazine-2-carboxylic acid ethyl ester (Intermediate B) (30 mg, 0.13 mmol) in acetic acid (5 ml), sodium carbonate (15 mg, 0.14 mmol) was added. To this mixture, half the contents of a solution of bromine (7 μΙ_, 0.13 mmol) in acetic acid (5 ml) were added, followed by the addition of sodium carbonate ((15 mg, 0.14 mmol). The remaining solution of bromine in acetic acid was added and the reaction mixture was left to stir at RT for 2 hours. The mixture was diluted with water and the resulting yellow precipitate was filtered under vacuum to afford the title compound.

Intermediate C: 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [0209] To a stirring solution of 3-amino-5-trifluoromethyl-pyrazine-2-carboxylic acid ethyl ester (10 g, 31.8 mmol) in ethanol (20 ml), 2M NaOH (20 ml, 31.8 mmol) was added.

[0210] The resulting solution was stirred at RT for 5 minutes and poured into water (50 ml). The pH was adjusted to pH6 with the addition of 1 M HCI. The resulting suspension was filtered under vacuum, washed with water (20ml) and dried to afford the title compound; MS m/z 287[M+H]+. 1H NMR (400 MHz, DMSO - d6) δ 7.98 (2H, s).

Intermediate D 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid [0211]

Intermediate D1 : 6-Bromo-3-(2,5-dimethyl-pyrrol-1-yl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester [0212]

[0213] 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A4) (2 g, 6.69 mmol) was suspended in toluene (8 ml), and treated with p-toluenesulfonic acid (TsOH) (0.115 g, 0.669 mmol) and acetony-lacetone (0.941 ml, 8.03 mmol). The reaction mixture was heated at reflux for 2 hours (using Dean-Stark apparatus) and allowed to cool to RT overnight. The resulting dark red/ black solution was concentrated in vacuo to remove toluene and the crude residue diluted with EtOAc(200 ml), washed with NaHC03 (50 ml), dried (MgS04) and concentrated in vacuo to give a brown solid. Purification of the solid by chromatography on silica eluting with EtOAc/iso-hexane afforded the title compound; LC-MS Rt = 5.58 min [M+H]+ 377/379 (Method 10minLC_v002). 1 H NMR (400 MHz, DMSO-d6) δ 8.50 (1 H, s), 7.77 (2H, s), 5.83 (3H,s), 1.90 (6H, s); 19F NMR(400 MHz, DMSO-d6) δ-62.26 (CF3, s).

Intermediate D2: 3-(2,5-Dimethyl-pyrrol-1-yl)-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid [0214]

[0215] 6-Bromo-3-(2,5-dimethyl-pyrrol-1-yl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (2 g, 5.30 mmol) was dissolved in MeOH (40 ml) and treated with 2M NaOH (20 ml) to give a suspension which was stirred at RT for 1h to afford a clear solution. The solvent was removed in vacuo and the resulting residue was acidified to pH1 with 5M HCI. The mixture was extracted with EtOAc (200 ml) and the organic extract was dried (MgS04) and concentrated in vacuo to afford the title compound as a dark brown solid which was used in the next step without further purification; LC-MS Rt=1.50 min [M+H]+ 315.2.1/316.2 (Method 2minLC_v002);1H NMR (400 MHz, DMSO-d6) Ô14.42-12.61 (COOH, b), 8.25 (1H, s), 5.84 (2H, s), 4.13 (3H, s), 1.97 (6H, s); 19F NMR (400 MHz, DMSO-d6) δ -62.43 (CF3, s).

Intermediate D: 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid [0216] 3-(2,5-Dimethyl-pyrrol-1-yl)-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (2.1g , 6.68 mmol) was dissolved in EtOH (40 ml) and water (20 ml). To this mixture was added TEA (2.79 ml, 20.05 mmol) followed by hydroxylamine hydrochloride (4.64g, 66.8 mmol). The resulting mixture was heated at reflux for 5 hours. After cooling to RT, the mixture was diluted with EtOAc (100ml) and washed with aqueous HCI (1 M, 100ml). The aqueous phase was back extracted with EtOAc (100ml) and the combined organic phases washed with brine (100ml), dried (MgS04) and concentrated in vacuo to afford the product as an orange solid. The material can be used crude or recrystallised from isohexane-EtOAc (10:1) LC-MS Rt =1.0 min [M+H]+ 237 (Method 2minLC_v003) 1 H NMR (400 MHz, DMSO-d6) δ 8.5 (NH2, b), 7.70 (1 H, s), 3.89 (3H, s).

Intermediate DA 3-(2,5-Dimethyl-1H-pyrrol-1-yl)-6-(pyrrolidin-1-yl)-5-(trifluoromethyl)picolinic acid [0217]

[0218] Step 1: 6-Bromo-3-(2,5-dimethyl-1 H-pyrrol-1-yl)-5-(trifluoromethyl)picolinic acid 6-Bromo-3-(2,5-dimethyl-pyr-rol-1-yl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (1.9 g, 5.04 mmol) and 2M NaOH (2.52 ml, 5.04 mmol) in THF (10 ml) was stirred at RT for 1 hour. The reaction mixture was poured into water (50ml) and the pH was adjusted to pH 4 by addition of 1 M HCI. The mixture was extracted with EtOAc (2 x 50ml) and the organic portion was washed with brine (30 ml), dried over MgS04 (5g), filtered and concentrated to give the title compound as a crystalline orange solid; LC_MS Rt=1.21 min [M+H]+ 363.1 (Method 2minLC_v003).

[0219] Step 2: 3-(2,5-Dimethyl-1 H-pyrrol-1-yl)-6-(pyrrolidin-1-yl)-5-(trifluoromethyl)picolinic acid To a stirring solution of 6-bromo-3-(2,5-dimethyl-1 H-pyrrol-1-yl)-5-(trifluoromethyl) picolinic acid (300 mg, 0.826 mmol) in THF (1 ml), pyrrolidine (0.136 ml, 1.652 mmol) was added. The orange solution was stirred at RT overnight. The reaction mixture was partitioned between 0.5M HCI (30ml) and EtOAc (30ml) and shaken. The organic portion was separted and washed with brine (30ml), dried over MgS04, filtered and concentrated in vacuo to give a red oil. The crude product was purified on silica eluting with 0-40% EtOAc in iso-hexane to afford the title product; 1 H NMR (400MHz, DMSO d6) δ 13.45 (1 H, br s), 7.88 (1 H, s), 5.74 (2H, s), 3.58 (5H, brs), 1.88 - 2.0 (11 H, unresolved peaks).

Intermediate DB 3-(2,5-Dimethyl-1H-pyrrol-1-yl)-6-ethoxy-5-(trifluoromethyl)picolinic acid [0220]

[0221] Step 1 : Methyl 3-(2,5-dimethyl-1H-pyrrol-1-yl)-6-methoxy-5-(trifluoromethyl)picolinate 3-(2,5-Dimethyl-pyrrol- 1-yl)-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (Intermediate D2)(500 mg, 1.591 mmol) in methanol (15.91 ml) was treated with H2S04 (0.0424 ml, 0.795 mmol) and the soltuion was heated at reflux for overnight.

[0222] The solvent removed was removed in vacuo and the resulting brown oil was neutralised to pH 7 using saturated sodium bicarbonate. The mixture was extracted with EtOAc (20 ml) and the combined organic extracts were washed with water (20 ml), brine (20 ml), passed though a phase separator and concentrated in vacuo. Purification of the crude product by chromatography on silica eluting with iso-hexane: EtOAc (gradient of 0 to 10 % EtOAc) afforded the title compound as an off-white powder. 1 H NMR (400 MHz, DMSO-d6) δ 8.3 (1 H, s), 5.8 (2H, s), 4.1 (3H, s), 3.6 (3H, s), 1.9 (6H, s).

[0223] Step 2: Methyl 3-(2,5-dimethyl-1 H-pyrrol-1-yl)-6-hydroxy-5-(trifluoromethyl)picolinate Methyl 3-(2,5-dimethyl-1 H-pyrrol-1-yl)-6-methoxy-5-(trifluoromethyl)picolinate (100 mg, 0.305 mmol) in acetonitrile (3.05 ml) was treated with Kl (202 mg, 1.218 mmol) and TMS-Chloride (0.156 ml, 1.221 mmol) and heated at reflux for 6 hours. The solvent removed was in vacuo and the crude product was dissolved in EtOAc (20 ml) and washed with water (2x 10 ml) and brine (10 ml), dried over a phase separator and concentrated in vacuo. Purification of the crude product by chromatography on silica eluting with iso-hexane: EtOAc (gradient of 0 to 30 % EtOAc) afforded the title compound as an yellow powder. LC-MS Rt = 1.11 mins [M+H]+ 315.4 (Method 2minLC_v003).

[0224] Step 3: Methyl 3-(2.5-dimethvl-1 H-pyrrol-1-yl)-6-ethoxy-5-(trifluoromethyl)picolinate Methyl 3-(2,5-dimethyl-1H-pyrrol-1-yl)-6-hydroxy-5-(trifluoromethyl)picolinate (62 mg, 0.168 mmol) in 1,4-dioxane (1.5 ml) (dry) was treated with EtOH (0.020 ml, 0.335 mmol) and triphenylphosphine (88 mg, 0.335 mmol) and the solution stirred. DEAD (0.053 ml, 0.335 mmol) was added dropwise and the reaction mixture stirred at room temperature for 2 hours. The solvent was removed in vacuo and purification of the crude product by chromatography on silica eluting with iso-hexane: EtOAc (gradient of 0 to 10 % EtOAc) afforded the title compound; 1 H NMR (400 MHz, DMSO-d6) δ 8.3 (1 H, s), 5.8 (2H, s), 4.5 (2H, q), 3.6 (3H, s), 1.9 (6H, s), 1.4 (3H, t).

[0225] Step 4: 3-(2,5-Dimethyl-1 H-pyrrol-1-yl)-6-ethoxy-5-(trifluoromethyl)picolinic acid Methyl 3-(2,5-dimethyl-1H-pyrrol-1-yl)-6-ethoxy-5-(trifluoromethyl)picolinate (140 mg, 0.409 mmol) was dissolved in THF (2.045 ml). NaOH (0.613 ml, 1.226 mmol) was added and heated at reflux for 6 hours. The solvent was removed in vacuo and the resulting mixture was diluted withEtOAc (25 ml) was acidified to pH 1 using HCl (5M). The organic portion washed with brine, dried using a phase separator and concentrated in vacuo to afford the title compound as a yellow oil. LC-MS Rt = 1.26 mins [M+H]+ 329.2 Method 2minLC_v003.

Intermediate E 3-Amino-5-trifluoromethvl-pvridine-2-carboxvlic acid [0226]

[0227] To a stirring solution of 3-Amino-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A3) (1 g, 4.54 mmol) in MeOH (20 ml) was added 2M NaOH (0.182 g, 4.54 mmol). The orange solution was stirred at RT for 1 minute and then into water (10 ml). The solution was acidified to pH 1 with the addition of 1 M HCI and the product was extracted with EtOAc (150ml). The organic portions were combined, washed with brine (50ml), dried over MgS04 and concentrated in vacuo to afford the title compound as an orange solid; LC-MS Rt = 0.82 mins [M+H]+ 207.1 (Method 2minLC_v002); 1 H NMR (400MHz, DMSO - d6) δ 13.9 (1H, broad hump), 8.11 (1 H, s), 7.59 (1 H, s), 7.08 (2H, broad hump) (trace of EtOAc present but correlates to proposed structure).

Intermediate G 3-Amino-6-(4-fluoro-phenvn-5-trifluoromethvl-pvridine-2-carboxvlic acid [0228]

[0229] A mixture comprising 3-amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (Intermediate A) (1 g, 3.51 mmol), 4-fluorophenylboronicacid (0.736 g, 5.26 mmol) and 1,1’Bis(diphenylphosphoshio)ferrocene palladium dichloride (0.286 g, 0.351 mmol) and 1,0M Cs2C03 (3.3ml) in THF (10 ml) was heated to reflux for 10 hours. After cooling to RT, the mixture was partitioned between DCM (100ml) and 1 M NaOH (2 x 100ml). The aqueous phase was acidified with 5M HCI and the resulting milky solution was extracted into DCM (2 x 100ml). The organic portion was separated, dried (MgS04) and concentrated in vacuo to afford the product as a crude oil. The crude material was purified by flash chromatography on silica cartridge eluting with a gradient of DCM: MeOH from 0% to 10% MeOH to afford the title product as a pale yellow solid; 1H NMR (DMSO-d6, 400MHz) δ 12.9 (1 H, br s, COOH), 7.7 (1H, s, CH, Ar-H), 7.4 (2H, m, Ar-H), 7.25 (2H, m, Ar-H), 7.1 (2H, br s, NH2).

Intermediate GA 3-Amino-6-cyclopropyl-5-(trifluoromethyl)picolinic acid [0230]

Step 1: 3-Amino-6-cyclopropyl-5-(trifluoromethyl)picolinic acid [0231] A microwave vial was charged with amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A4) (0.5 g, 1.754 mmol), cyclopropylboronicacid (0.753 g, 8.77 mmol), and 1 ,TBis(diphenylphosphosphino) ferrocene palladium dichloride (0.143 g, 0.175 mmol). The mixture was taken up as a solution in THF (6 ml) and flushed with N2, sealed and heated using microwave radiation at 150°Cfor20 minutes. The reaction mixture was filtered through Celite® (filter material) and washed through with EtOAc (20ml). The filtrate was partitioned between EtOAc (30ml) and water (50ml). The phases were separated and the organic portion was washed with brine (30ml), dried over MgS04, filtered and concentrated under vacuum.

[0232] The crude material was taken up in EtOAc (20ml) and dry loaded onto silica (2 - 3g). Material then purified on the Combiflash Rf Teledyne ISCO System 100% Isohexane to 60% EtOAc: I so hexane to afford semi pure material which was used without further purification.

Step 2: 3-Amino-6-cyclopropyl-5-(trifluoromethyl)picolinic acid [0233] To a stirring solution of 3-amino-6-cyclopropyl-5-(trifluoromethyl)picolinic acid (472 mg, 1.814 mmol) in THF (10 ml), 2M NaOH (10 ml, 20.00 mmol) was added. The orange solution was stirred at RT for 2 days. The reaction mixture waspoured into water (30 ml) and the pH adjusted to pH6 with the addition of 1 M HCI. The product was extracted with EtOAc (50 ml) and the organic portion was dried over MgS04, filtered and concentrated in vacuo to give the title compound as a red/orange oil. LC-MS Rt = 1.10 mins [M+H]+ 247.1 (Method 2minLC_v003);

Intermediate H 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid [0234]

Intermediate H1 : 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester [0235] 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylicacid methyl ester (Intermediate A4) (3g, 10.03 mmol), 2,4-dichlorophenylboronicacid (2.297 g, 12.04 mmol), potassium phosphate (4.26 g, 20.06 mmol) and Fibrecat® 1034A (Johnson Matthey, polymer supported palladium complex) (500mg, 10.03 mmol) were suspended in toluene (50 ml) and water (15 ml). The reaction mixture was heated to 110°C under vigorous stirring for 3 hours. The mixture was allowed to cool to RT and EtOAc (100 ml) was added. The organic layer was separated and washed with brine (15 ml). MP-TMT (macroporous polystyrene-bound trimercaptotriazine ,3 g, Polymern labs) was added and stirred for 1 hour at RT. MgS04 was added and the suspension filtered off. The filtrate was concentrated in vacuo and purification of the residue by reverse phase chromatography (130g C18 column) eluting with water/MeOH afforded the title compound as a white solid; LS-MS Rt = 1.55 mins [M+H]+ 365 (Method 2minLC_v002).

Intermediate H: 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid [0236] 3-Amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (0.9 g, 2.465 mmol) was suspended in MeOH (15 ml) and NaOH 2M (2.465 ml, 4.93 mmol) was added under stirring. 1,4-Dioxane (15.00 ml) was added and the solution was left standing at RT over night. The solvent was removed in vacuo and the resulting residue was dissolved in water (10 ml) and carefully acidified to pH4 with slow addition of 2M HCI (2 ml) whilst stirring. The mixture was extracted with EtOAc (20 ml) and the organic portion was washed with brine and concentrated in vacuo. The residue was purified by reverse phase chromatography (130g C18 column) eluting with water/MeOH to afford the title compound; LS-MS Rt = 1.57 mins [M+HJ+351.0 (Method 2minLC_v002).

Intermediate I 3-Amino-6-(4-chloro-2-methyl-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester [0237]

[0238] This compound was prepared from 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A4) and 4-chloro-2-methylphenylboronicacid analogously to Intermediate H; LC-MS Rt=1.53 mins, [M+H]+ 331 (Method 2minLC_v002).

Intermediate J 2-Aminomethyl-1,1,1,3,3,3-hexafluoro-propan-2-ol [0239] To a stirred mixture of 35% ammonium solution (1 ml) and diethyl ether (1 ml) was added 3,3,3-trifluoro-2-(trifluoromethyl)-1,2-propenoxide (500 mg, 2.78 mmol) dropwise and the reaction mixture was left to stir at RT for 3 hours. The reaction mixture was separated and the aqueous layer was extracted with diethyl ether (2x3 ml). The combined organic portions were dried (MgS04) and concentrated in vacuo to give a white crystalline solid; 1 H NMR (400 MHz, DMSO-d6) δ 4.20 (broad), 3.30 (broad), 3.15 (s), 3.02 (s), 2.50 (s, DMSO). 19F NMR (400 MHz, DMSO-d6) δ-85 (CF3),-84.5 (CF3).

Intermediate K 5-Amino-6’-methyl-3-(trifluoromethyl)-2,3’-bipyridine-6-carboxylic acid [0240]

Intermediate K1 : 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid methyl ester [0241] This compound was prepared from 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A4) and 2-methylpyridine-5-boronic acid analogously to 3-amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-py-ridine-2-carboxylic acid (Intermediate G); LC-MS Rt 0.96 min [M+H]+ 312 (Method 2minLC_v002); 1 H NMR (400 MHz, DMSO-d6) δ 8.41 (1 H, s), 7.79 (1 H, s), 7.69 (1 H, dd), 7.32 (1 H, d), 7.10 (2H, s), 3.82 (3H, s), 2.52 (3H, s).

Intermediate K: 5-Amino-6’-methyl-3-(trifluoromethyl)-2,3’-bipyridine-6-carboxylic acid [0242] This compound was prepared from : 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl6-carboxylic acid methyl ester analogously to 3-amino-6-(4-chloro-2-methyl-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate I); LC-MS Rt 0.90 min; [M+H]+ 298 (Method 2minLC_v002); 1 H NMR (400 MHz, DMSO-d6) δ 12.90 (1 H, broad), 8.45 (1 H, s), 7.72 (2H), 7.32 (1 H, d), 7.12 (2H, broad), 2.51 (3H).

Intermediate KA 5-Amino-3-(trifluoromethyl)-2,4’-bipyridine-6-carboxylic acid [0243]

[0244] The title compound was prepared analogously to Intermediate K using the appropriate boronic acid in step 1 ; 1 H NMR (400 MHz, DMSO-d6) δ 13.00 (1 H, broad), 8.65 (2H, d), 7.65 (1 H, s), 7.43 (2H, d), 7.18 (2H, broad).

Intermediate Μ 3-(2,5-Dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid [0245]

Intermediate M1 : 3-(2,5-Dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid methyl ester [0246] A stirred mixture of KF (2.12 g, 5.62 mmol) and Cul (0.490 g, 8.43 mmol) was heated in a sealed 10.0 - 20.0 ml microwave vial under vacuum until a slight greenish colour began to appear. The vial was then placed under nitrogen to cool. A solution 6-bromo-3-(2,5-dimethyl-pyrrol-1-yl)-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate D) (2.64 ml, 16.86 mmol) in 1:1 dry DMF/dry NMP (14 ml) was then added, followed by TMS-CF3 (2.64 ml, 16.86 mmol). A new septum was then used to seal the vial and the reaction mixture was heated using microwave radiation with stirring at 100 °C for 3 h and allowed to cool. The mixture was added to 5M NH3 solution (50 ml) and then extracted with diethyl ether (4 x 50 ml). The combined organic extracts were washed with 5M NH3 solution (3 x 20 ml), 1M HCI (50 ml), sat. sodium bicarbonate solution (2 x 50 ml), brine (50 ml), dried (MgS04) and concentrated in vacuo to give a brown oil. The crude material was purified by chromatography on silica eluting with Iso-hexane / EtOAc, 0-10 % to afford the title compound as an orange solid; LC-MS Rt 1.37 min; MS m/z 367.1 [M+FI]+; Method 2minLC_v003.

Intermediate M: 3-(2,5-Dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid [0247] To a stirred solution 3-(2,5-dimethyl-pyrrol-1-yl)-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (1.28 g, 3.49 mmol) in methanol (25 ml) was added 1 M NaOFI (7 ml, 6.99 mmol) and the reaction mixture was left to stir at RT for 30 min. The solvent was removed in vacuo and water (20 ml) was added to the remaining residue. The pH was adjusted to pH 4/5 by the addition of 1 M HCI. The mixture was extracted with EtOAc (3 x 20 ml) and the combined organic extracts were washed with brine (30 ml), dried (MgS04) and concentrated in vacuo and dried in a vacuum oven (50 °C) overnight to give the crude title product as an orange solid which was used without further purification; LC-MS: Rt 1.23 min; MS m/z 353.1 [M+H]+; Method 2minLC_v003.

Intermediate N 3,3,3-Trifluoro-N2-(4-methoxybenzyl)-2-methylpropane-1,2-diamine [0248]

Step 1: 1-(4-methoxyphenyl)-N-(1,1,1-trifluoropropan-2-ylidene)methanamine [0249] To a stirring solution of trifluoroacetone (7.75 g, 69.2 mmol) in diethyl ether (60 ml) at - 40°C was added 4-methoxybenzyl amine (9.49 g, 69.2 mmol) and triethylamine (14 g, 138 mmol) in diethyl ether (40 ml). A cooled (0°C) mixture of TiCI4 (6.56 g, 34.6 mmol) in hexane (40 ml) at was added dropwise over 10 minutes and the resulting mixture was allowed to warm up to ambient temperature over 20 mins and stirred at 50°C for 2.5h. The inorganic precipitate was removed by filtration and washed with diethyl ether. The filtrate was concentrated in vacuo to afford a yellow oil. Purification of the crude product by chromatography on silica eluting with 0% to 25% EtOAc in iso-hexane afforded the title product.

Step 2: 3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methyl propanenitrile [0250] To a cooled (0°C) solution of 1-(4-methoxyphenyl)-N-(1,1,1-trifluoropropan-2-ylidene)methanamine (4.41 g, 19.07 mmol) in DCM (100 ml) was added cyanotrimethylsilane (2.84 g, 28.6 mmol) and magnesium bromide. The mixture was stirred at RT for 90 h and then diluted with sat. NaHC03 (200 ml). After stirring at RT for 1 h, the organic phase was separated, washed with a further portion of sat. NaHC03 (100 ml), dried over MgS04 and concentrated in vacuo to afford the title compound. Step 3: 3,3,3-trifluoro-N2-(4-methoxybenzyl)-2-methylpropane-1,2-diamine [0251] To a cooled (0°C) solution of 3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methyl propanenitrile (1.5 g, 5.81 mmol) in dry diether ether (50 ml) was added LÍAIH4 (11.62 ml of a 2M solution in THF) and the resulting mixture was stirred at RT overnight. The reaction mixture was hydrolyzed by successive addition of water 15% KOH, and water. The resulting precipitate was filtered on Celite® (filter material) and the organic portion was washed with water, dried over MgS04 and concentrated under reduced pressure to afford the title product; 1 H NMR (400 MHz, Methanol-d4) δ 7.97 (1 H, s), 7.85 (1 H, s), 7.60 (1 H, s), 3.97 (3H, s), 3.77 (1 H, m), 3.56 (1 H, m), 1.37 (3H, s) LC-MS: Rt 3.22 min; MS m/z 412.3 [M+H]+; Method 10minLC_v003.

Intermediate O

Benzo[d]isoxazol-3-ylmethanamine [0252]

[0253] The title compound was prepared according to the procedure of Pigini, Maria; Giannella, Mario; Gualtieri, Fulvio; Melchiorre, Carlo; Bolle, Paola; Angelucci, Luciano. Analogs with a 1,2-benzisoxazole nucleus of biologically active indole derivatives. III. Tryptamine and gramine isosteres. European Journal of Medicinal Chemistry (1975), 10(1), 29-32 (Compound 11 page 31-32).

Intermediate P

Methyl 3-amino-6-(oxazol-2-yl)-5-(trifluoromethyl)picolinate [0254] A solution of 3-amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid methyl ester (Intermediate A4) (500 mg, 1.672 mmol), 2-(tributylstannyl)oxazole (0.704 ml, 3.34 mmol) and tetrakis(triphenyiphosphine)pailadium(0) (193 mg, 0.167 mmol) in dioxane (10 ml) was heated at reflux for 13 hours. After cooling to room temperature over 8 hours, the solvent was evaporated and the resulting residue triturated with hot methanol to remove a yellow solid impurity. The remaining crude material was used without further purification. LC-MS: Rt 0.95 min; MS m/z 288 [M+H]+; Method 2minLC_v003.

Intermediate PA 3-Amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid [0255]

Step 1: 3-Amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid [0256] The title compound was prepared from 3-amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid ethyl ester (Intermediate C1) and tributyltin-2-furylstannane analogously to methyl 3-amino-6-(oxazol-2-yl)-5-(trifluoromethyl)pi-colinate (Intermediate P)

Step 2: 3-Amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid [0257] The title compound was prepared from 3-amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid and 6M NaOH analogously to 3-amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (Intermediate C, final step).

Intermediate Q 2-Hydroxy-3-methyl-2-(trifluoromethyl)butan-1-aminium chloride [0258]

Step 1: 1,1,1-Trifluoro-3-methyl-2-(nitromethyl)butan-2-ol [0259] A cooled (0 °C) solution of lithium hydroxide (0.048 g, 2.015 mmol) in water (20 ml) was stirred and treated with nitromethane (1.23 g, 20.15 mmol), 1,1,1-trifluoro-3-methylbutan-2-one (3.11 g, 22.17 mmol), cetyltrimethylammo-nium Chloride (0.871 g, 2.72 mmol) and MgS04 (0.485 g, 4.03 mmol). The white suspension was stirred at 0 °C for 1 hr, then at RT for 2 days. The resulting biphasic mixture was separated and the more dense lower layer was collected and dissolved in diethyl ether (30ml). The mixture was dried over MgS04, filtered and concentrated in vacuo to give a pale yellow oil. The oil was taken up in diethyl ether (10ml) and passed through a pre-packed SCX-2 cartridge eluting with 100% diethyl ether. The filtrate was concentrated in vacuo to afford the title compound as a colourless oil. 1H NMR (400MHz, CDCI3): δ 4.74 (1H, d), 4.59 (1 H, d), 4.29 (1 H, s), 2.29 (1 H, m), 1.1 (6H, two sets of unresolved doublets)

Step 2: 2-Hydroxy-3-methyl-2-(trifluoromethyl)butan-1-aminium chloride [0260] To a solution of 1,1,1-trifluoro-3-methyl-2-(nitromethyl)butan-2-ol (753 mg, 3.74 mmol) in EtOH (10 ml) in a 25ml medium pressure glass hydrogenation vessel under N2, 10% Pd on carbon (39.8 mg, 0.374 mmol) was added. The vessel was flushed with N2, followed by H2 (22.64 mg, 11.23 mmol) at 5 bar pressure and stirred at RT for 6 days. The mixture was filtered through Celite® and washed through with EtOH(30ml), followed by DCM (10ml). The filtrate was concentrated under vacuum to give a colourless oil. The crude product was taken up in methanol (20 ml) and treated with a 1.25M HCI in methanol solution. The resulting colourless solution was stirred at RT for 1 hour and concentrated under vacuum to afford the title compound; 1 H NMR (400MHz, DMSO - d6) δ 8.04 (3H, broad peak), 6.74 (1 H, s), 3.58 (broad peak), 3.6 (2H, m), 2.12 (1 H, m), 0.99 (6H).

Intermediate R 3-Amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride [0261]

Step 1: 1,1,1-trifluoro-2-methyl-3-nitropropan-2-ol [0262] To LiOH (0.193 g, 8.06 mmol) in a 3-neck roundbottom flask was added water (25 ml), nitromethane (3.76 ml, 81 mmol) and trifluoroacetone (7.95 ml, 89 mmol). Cetyltrimethylammonium chloride (3.8 g, 10.88 mmol) and MgS04 (1.9 g, 16.12 mmol) were added and the resulting yellow solution stirred at 20-25 °C for 2 days. The reaction mixture was poured into diethyl ether (120 ml) and washed with water (3x 200 ml) and brine (1x 100 ml). The organic portion was dried over MgS04 and concentrated in vacuo to afford the title compound as a yellow liquid. 1 H NMR (CDCI3, 400 MHz): δ 4.7 (1 H d), δ 4.5 (1H, d), δ 3.7 (1H, broad), δ 1.6 (3H, si. Step 2: 3-Amino-1.1.1-trifluoro-2-methvlpropan-2-ol hydrochloride [0263] Pd/C was added (1 g) to a 200 ml glass vessel. Ethanol (50 ml, dry) was added cautiously under an atmosphere of C02. 1,1,1-Trifluoro-2-methyl-3-nitropropan-2-ol (10 g, 57.8 mmol) was dissolved in ethanol (50 ml, dry) and added to the glass vessel. The reaction mixture was put under a positive pressure of hydrogen (5 bar) at room temperature and hydrogenated for 2 days. The reaction mixture was filtered through Celite®(filter material) and washed with excess ethanol. The solvent was removed in vacuo to yield a colourless oil. The oil was dissolved in MeOH (50 ml) and treated dropwise with HCI (1 M) in MeOH (30 ml). The solution was Iefttostirfor30 minutes and concentrated in vacuo azeotroping with MeCN to afford the title compound as a waxy white solid; 1 H NMR (DMSO-d6, 400MHz) δ 8.3 (3H, broad s), 6.9 (1 H, broad), 3.0 (2H, q), 1.4 (3H, s).

Intermediate RA (S)-3-Amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride [0264]

Step 1: Benzyl 3,3,3-trifluoro-2-hydroxy-2-methylpropylcarbamate [0265] To a stirring suspension of amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride (Intermediate R) (1.5 g, 8.35 mmol) in DCM (50 ml) was added TEA 93.54 g, 35.0 mmol) followed by benzyl 2,5-dioxopyrrolidin-1-yl carbonate (1.983 g, 7.96 mmol). The mixture was stirred at RT for 6 hours and then diluted with water. The organic portion was separated using a phase separator and concentrated in vacuo. Purification by chromatography on silica eluting with 0-70% EtOAc in iso-hexane afforded the title product; 1 H NMR (400 MHz, DMSO-d6) δ 7.34 (6H, m), 5.98 (1 H, s), 5.05 (2H, s), 3.31 (1H, m), 3.18 (1 H, m), 1.21 (3H. s)LC-MS: Rt 1.05 min; MS m/z 278.1 [M+H]+; Method 2minLC_v003.

Step 2: Separation of Enantiomers of benzyl 3,3,3-trifluoro-2-hydroxy-2-methyl propylcarbamate [0266] Benzyl 3,3,3-trifluoro-2-hydroxy-2-methylpropylcarbamate (1.7 g) was dissolved in 2-propanol (10 ml) and purified using the following chromatographic conditions:

Mobile Phase: 10% 2-propanol / 90% C02

Column: 2xChiralcel OJ-H, 250x 10 mm id, 5 μίτι (columns coupled in series)

Detection: UV @ 220nm

Flowrate: 10ml/min

Sample concentration: 1.7 g in 10 ml 2-propanol Injection volume: 75μΙ [0267] First eluted peak: Rt = 6.94 minutes (R)-benzyl 3,3,3-trifluoro-2-hydroxy-2-methyl propylcarbamate [0268] Second eluted peak: Rt = 8.04 minutes (S)-benzyl 3,3,3-trifluoro-2-hydroxy-2-methyl propylcarbamate (Stereochemistry confirmed by analysis of final compound prepared by subsequent steps)

Step 3: (S)-3-Amino-1,1,1-trifluoro-2-methylpropan-2-ol hydrochloride [0269] A mixture comprising (S)-benzyl 3,3,3-trifluoro-2-hydroxy-2-methyl propylcarbamate in EtOH(165 ml) was pumped through a Η-Cube (hydrogenation reactor, 1-2 ml/min, 1 bar pressure, RT) for 8 hours using a 10% palladium on carbon catalyst cartridge. 1.25 M HCI in methanol (130 ml) was added to the mixture was stirred for 30mins. The solvent was removed in vacuo azeotroping with MeCN to afford the title product as a white powder; 1 H NMR (400 MHz, DMSO-d6) δ 8.3 (3H, broad), 6.8 (1 H, s), 3.0 (2H, s), 1.5 (3H, s).

[0270] Alternatively, racemic 3-Amino-1,1,1-trifluoro-2-methylpropan-2-ol can be resolved into separate enantiomers by recrystallistion with either (S)-Mandelic acid or L-tartaric acid in isopropanol or ethanol.

Intermediate S 2-Aminomethyl-1,1,1,3,3,3-hexafluoro-propan-2-ol [0271] 3,3,3-Trifluoro-2-(trifluoromethyl)-1,2-propenoxide(1 g, 5.55 mmol) was added to a stirred solution of aqueous ammonia solution (0.88 g/ml, 3 ml) and diethyl ether (3 ml). The resulting colourless solution was stirred at room temperature for 3 hours. The biphasic mixture was separated and and the aqueous portion was further extracted with diethyl ether (2x5 ml). The combined organic layers were dried over MgS04 and concentrated in vacuo (no heating) to afford the title compound as a white crystalline solid which was used without further purification; 1 H NMR (400 MHz, DMSO-d6) signals unassigned δ 4.20 (broad), 3.15 (s).

Intermediate T 3,3,3-Trifluoro-2-methoxy-2-methylpropan-1-amine [0272]

Step 1: 2-(3,3,3-Trifluoro-2-hydroxy-2-methylpropyl)isoindoline-1,3-dione [0273] A mixture comprising 3,3,3-trifluoro-2-hydroxy-2-methyl-propyl-ammonium (0.9g), phthalic anhydride (1.039 g) and DIPEA (2.188 ml) in chloroform (30 ml) was heated at 70°C for 5 hours. After cooling to RT, the mixture was washed with water and passed through a phase separator. The organic phase was reduced to dryness. The crude product was purified by chromatography on silica, eluting in a 0% to 30% iso-hexane :EtOAc removed to give the title product; 1H NMR (400 MHz, Methanol-d4) δ 7.92 (2H, m), 7.85 (2H, m), 3.95 (2H, m), 1.36 (3H, s).

Step 2: 2-(3,3,3-Trifluoro-2-methoxy-2-methylpropyl)isoindoline-1,3-dione [0274] To a stirring solution of2-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)isoindoline-1,3-dione (250 mg, 0.915 mmol)) at 0°C in THF (8 ml), NaH (80 mg, 2 mmol) was added. After 30 minutes methyl iodide (1.299, 9.15 mmol) was added. The reaction mixture was left stirring in a ice-bath and allowed to warm to 25°C over 3.5 hours. The reaction was quenched with sat. NH4CI and the mixture extracted with DCM. The organic extract was separated using a phase seperator and purification by chromatography on silica, eluting in a 0% to 30% iso-hexane :EtOAc afforded the title product; 1 H NMR (400 MHz, Methanol-d4) δ 7.91 (2H, m), 7.85 (2H, m), 3.97 (2H, m), 3.44 (3H,s), 1.42 (3H, s); LC-MS: Rt 1.17 min; MS m/z 288.10 [M+H]+; Method 2minLC_v003.

Step 3: 3,3,3-Trifluoro-2-methoxy-2-methylpropan-1-amine [0275] A mixture comprising 2-(3,3,3-trifluoro-2-methoxy-2-methylpropyl)isoindoline-1,3-dione (272 mg, 0.95 mmol) and hydrazine (0.033 ml, 1.045 mmol) was stirred at 75°C for 4 hours. After cooling to RT, the mixture was filtered and the filtrate was concentrated in vacuo to afford the title product which was used without further purification (no characterisation data available).

[0276] From the foregoing it will be appreciated that, although specific embodiments of the invention have been described herein for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention. Accordingly, the invention is not limited except as by the appended claims.

Embodiments/Consistorv Clauses [0277]

Embodiment 1: A compounds according to Formula I:

or pharmaceutically acceptable salts thereof, wherein: A is N or CR4a; R1 is H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; C.|-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; S02NR8R9; S02R10; S-C1-C8alkyl optionally substituted by one or more halogen atoms; S-C6-C14 aryl; -(C0-C4 alkyl)-C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; CN; NR11R12; CONR13R14; NR13S02R15; NR13C(0)R15 and C02R15, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; R2 is CyC4 haloalkyl; R3, R4 and R4a are each independently H or C.|-C8 alkyl optionally substituted by one or more halogen atoms; R5 and R6 are each independently H; C^-Cq alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -CVC4 alkyl-C3-C8 cycloalkyl; C1-C8 alkoxy optionally substituted by one or more halogen atoms; OH; CN; halogen; -(C0-C4alkyl)-C6-C|4aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; or-(C0-C4 alkyl)-C02R15, wherein the cycloalkyl, cycloalkenyl, -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group groups are each optionally substituted by one or more Z substituents; or R5 and R6 are each independently a group of the formula: "(CH2)m-NR17Ri8; or R5 and R6 are each independently a group of the formula: -(CH2)m-OR4; or R4 and R5 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 8 membered carbocyclic ring system or a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R4, R5 and R6 cannot all be the same; m is 0, 1,2 or 3; R8, R11, R13 and R17 are each independently H, C.|-C8 alkyl optionally substituted by one or more halogen atoms, C3-C10 cycloalkyl or -(C.|-C4 alkyl)-C3-C8 cycloalkyl; R9, R10, R12, R14, R15, R16 and R18 are each independently H; C-|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R8 and R9, R11 and R12, R13 and R14, and R17 and R18 together with the nitrogen atom to which they are attached may form a 4 to 14 membered heterocyclic group optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, benzyl, O-benzyl, 0-,-Cg alkyl optionally substituted by one or more OH groups or NH2 groups, 0-,-Cg alkyl optionally substituted by one or more halogen atoms, 0-,-Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21, NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, CN, N02, halogen or a 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; R19and R21 are each independently H; C^Cg alkyl; C3-C8cycloalkyl; C1-C4alkoxy-C1-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from 0-,-Cg alkyl, 0-,-Cg alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, 0.,-Cg alkyl and C(0)C.|-Cg alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from 0-,-Cg alkyl, 0-,-Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, 0-,-Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, C.|-C4 alkoxy, C(0)NH2, C(0)NHC.|-Cg alkyl or C(0)N(C.|-Cg alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, O and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C1-C6 alkyl; 0-,-Cg alkyl optionally substituted by one or more halogen atoms; 0-,-Cg alkoxy optionally substituted by one or more OH groups orC1-C4 alkoxy; and 0(0)00.,-C6 alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by 0.,-Cg alkyl, 0.,-Cg haloalkyl or 0.,-Cg alkoxy.

Embodiment 2: The compound of Formula I

or pharmaceutically acceptable salts thereof, wherein: A is N or CR4a; R1 is H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C-|-C4 alkyl-C3-C8 cycloalkyl; C-|-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; S02NR8R9; S02R10; S-C1-C8alkyl optionally substituted by one or more halogen atoms; S-C6-C14 aryl; CN; NR11R12; C(0)NR13R14; NR13S02R15; NR13C(0)R15, C02R15, -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; R2 is CrC4 haloalkyl; R3 and R4a are each independently H or C1-C8 alkyl optionally substituted by one or more halogen atoms; R4 is H, or C1-C8 alkyl optional substituted with one or more halogen; R5 is -(CH2)m-NR17R18, -(CH2)m-OR’; C.,-C8 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C02R15; -(C0-C4 alkyl)-C6-C14 aryl or -3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4alkyl)-3to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; R6 is C-|-C8 alkyl optionally substituted by one or more halogen atoms; C3-C10 cycloalkyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; C1-C8 alkoxy optionally substituted by one or more halogen atoms; OH; CN; halogen; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; or R6 is H, and R5 is -(CH2)m-NR17R18, -(CH2)m-OR\ C1-C8 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; or -(C0-C4 alkyl)-C02R15, wherein -(C0-C4 alkyl)-C6-C14aryl and -(C0-C4alkyl)-3 to 14 membered heterocyclic group groups are each optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R4 and R5 togetherform an oxo group (C=0) and R6 is CrC4 alkyl optionally substituted by one or more halogen atoms; C.|-C4 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; or-(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R5 and R6 together with the carbon atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; or R4 and R5 and R6 together with the carbon atoms to which they are bound form a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R is H, or C-|-C8 alkyl optional substituted with one or more halogen; m is 0, 1,2 or 3; R8, R11, R13 and R17 are each independently H, C1-C8 alkyl optionally substituted by one or more halogen atoms, C3-C10 cycloalkyl or -(C.|-C4 alkyl)-C3-C8 cycloalkyl; R9, R10, R12, R14, R15, R16 and R18 are each independently H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C^-C4 alkyl-C3-C8 cycloalkyl; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R8 and R9, R11 and R12, R13 and R14, and R17 and R18 together with the nitrogen atom to which they are attached may form a 4 to 14 membered heterocyclic group optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, benzyl, O-benzyl, C^Cg alkyl optionally substituted by one or more OH groups or NH2 groups, C^Cg alkyl optionally substituted by one or more halogen atoms, C^Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21, NR18C(0)R21, C(0)NR19R21, NR180(O)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, CN, N02, halogen or a 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; R19 and R21 are each independently H; C^Cg alkyl; C3-C8cycloalkyl; C1-C4 alkoxy-C1-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, C^Cg alkyl and 0(0)0-|-06 alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from 0.,-Cg alkyl, 0.,-Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, 0-,-Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, C.|-C4 alkoxy, C(0)NH2, C(0)NHC.|-Cg alkyl or C(0)N(C.|-Cg alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, 0 and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C1-C6 alkyl; 0-,-Cg alkyl optionally substituted by one or more halogen atoms; 0-,-Cg alkoxy optionally substituted by one or more OH groups orC1-C4 alkoxy; and 0(0)00.,-C6 alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by 0.,-Cg alkyl, 0.,-Cg haloalkyl or 0.,-Cg alkoxy.

Embodiment 3: The compound according to embodiment 1 or 2, wherein R1 is H; CrC4 alkyl optionally substituted by one or more halogen atoms; 0,-04 alkoxy optionally substituted by one or more halogen atoms; halogen; C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; or -NR11R12, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents.

Embodiment 4: The compound according to embodiment 1 to 3, wherein R1 is C-|-C4 alkyl optional substituted by one or more halogen atoms.

Embodiment 5: The compound according to embodiment 1 to 4, wherein R1 is -CH3 or CF3.

Embodiment 6: The compound according to embodiment 1,2 or 3, wherein R1 is CrC4 alkoxy optional substituted by one or more halogen atoms.

Embodiment 7: The compound according to embodiment 1,2, 3 or 6, wherein R1 is -OCH3, -OCH2CH3 or -OCF3.

Embodiment 8: The compound according to embodiments 1, 2 or 3, wherein R1 is aryl, wherein aryl is phenyl optionally substituted by one or more Z substituents,

Embodiment 9: The compound according to embodiment 1, 2, 3 or 8, wherein R1 is 4-fluorophenyl, 4-chloro-2-methylphenyl, or 2,4-dichlorophenyl.

Embodiment 10: The compound according to embodiment 1,2 or 3, wherein R1 is pyridyl, oxazole, pyrrolidine or pyrazole and is optionally substituted by one or more Z substituents.

Embodiment 11: The compound according to embodiment 1,2, 3 or 10, wherein R1 is 1-methyl-4-pyridyl, oxzaoyl-2-yl, 1-methyl-1 H-pyrazole-4-yl or pyrrolidin-1yl.

Embodiment 12: The compound according to embodiment 1 to 11, wherein R1 is Br,-CH3, -CF3, -OCH3, -OCH2CH3, -OCF3,4-fluorophenyl, 4-chloro-2-methylphenyl, 2,4-dichlorophenyl, 1-methyl-4-pyridyl, 1-methyl-1 H-pyrazole-4-yl, oxzaoyl-2-yl, or pyrrolidin-1yl.

Embodiment 13: The compound according to embodiment 1 to 12, wherein R5 provides a heteroatom two carbons from the amide nitrogen, wherein the heteroatom is oxygen or nitrogen.

Embodiment 14: The compound according to embodiment 1 to 13, wherein R4 is H or CrC4 alkyl optionally substituted by one or more halogen atoms; R5 is C.|-C4 alkoxy optionally substituted by one or more halogen atoms; -(CH2)m-NR17R18; -(CH2)m-OR\ or OH; R is H, or C1-C4 alkyl optional substituted with one or more halogen; m is 0, 1 or 2; R6 is CrC4 alkyl optionally substituted by one or more halogen atoms; CrC4 alkoxy optionally substituted by one or more halogen atoms; OH; ON; halogen; -(C0-C4 alkyl)-C6 aryl; or -(C0-C4 alkyl)-5 to 6 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R5 and R6 together with the carbon atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R17 and R18 are each independently H; or C.|-C4 alkyl optionally substituted by one or more halogen atoms.

Embodiment 15: The compound according to any proceeding embodiment, wherein R3 is H; R4 is H or Me; R4a is H; R5 is -(CH2)m-NR17R18; -(CH2)m-OR’; or OH; m is 0, or 1 ; R’isH; R6 is C.1-C4 alkyl optionally substituted by one or more halogen atoms; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 6 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; and R17 and R18 are each independently H; or C^-C4 alkyl optionally substituted by one or more halogen atoms.

Embodiment 16: The compound according to any proceeding embodiment, wherein R3 is H; R4 is H or Me; R4a is H; R5 is -NR17R18; or OH; R6 is C-|-C4 alkyl optionally substituted by one or more halogen atoms; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 6 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; and R17 and R18 are each independently H; or CyC4 alkyl optionally substituted by one or more halogen atoms.

Embodiment 17: The compound according to any proceeding claim, wherein R3 is H; R4 is H or Me; R4a is H; R5 is -NR17R18; or OH; R6 is C.1-C4 alkyl optionally substituted by one or more halogen atoms; and R17 and R18 are each independently H; or C-|-C4 alkyl optionally substituted by one or more halogen atoms.

Embodiment 18: The compound according to embodiment 1 to 13, wherein R3 is H; R4a is H; R4 and R5 form an oxo group; R6 is C.1-C4 alkyl optionally substituted by one or more halogen atoms; C^-C4 alkoxy optionally substituted by one or more halogen atoms; phenyl; or 5 to 6 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the phenyl and heterocyclyl groups are each optionally substituted by one or more Z substituents.

Embodiment 19: The compound according to embodiment 1 to 13 or 18, wherein R3 is H; R4a is H; R4 and R5 form an oxo group; R6 is CyC4 alkyl optionally substituted by one or more halogen atoms; or phenyl, wherein the phenyl is optionally substituted by one or more Z substituents; Z is independently OH, C.1-C4 alkyl optionally substituted by one or more OH groups or NH2 groups, C.1-C4 alkyl optionally substituted by one or more halogen atoms, C.1-C4 alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, C(0)0R19, C(0)R19, OR19, CN, or halogen; R19 is H; C.1-C4 alkyl; C3-C6 cycloalkyl; or C.1-C4 alkoxy-0^04 alkyl, wherein all alkyl are optionally substituted with halogens.

Embodiment 20: The compound according to embodiment 1 to 13 or 18 to 19, wherein R3 is H; R4a is H; R4 and R5 form an oxo group; R6 is Cf-C4 alkyl optionally substituted by one or more halogen atoms; or phenyl, wherein the phenyl is optionally substituted by one or more Z substituents; Z is independently, CyC4 alkyl optionally substituted by one or more halogen atoms, CyC4 alkoxy or halogen. Embodiment 21. The compound according to embodiment 1 to 13, wherein the compound is represented by formula II,

or a pharmaceutically acceptable salt thereof, wherein, R101 is selected from the following:

Embodiment 22: The compound according to embodiment 21, wherein R3 is H; R101 is

or

Embodiment 23: The compound according to embodiment 21, wherein R3 is H; R101 is or

Embodiment 24: The compound according to embodiment 21, wherein R3 is H; R101 is

Embodiment 25: The compound according to embodiment 21, wherein R3 is H; R101 is

or

Embodiment 26: The compound according to embodiment 1 to 13, wherein R3 is H; R101 is -{C-\-C2 alkyl)-5 to 10 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents.

Embodiment 27: The compound according to embodiment 21 or 26, wherein R3 is H; R101 is

Embodiment 28: The compound of formula III

III or pharmaceutically acceptable salts thereof, wherein: A is N or CR4a; X is NRy or O; R1 is C-|-C8 alkyl optionally substituted by one or more halogen atoms; C3-C10 cycloalkyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; C1-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; CN; NR11R12; C(0)NR13R14; NR13C(0)R15, C02R15, -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; R2 is CrC4 haloalkyl; R3 and R4a are each independently H or C1-C8 alkyl optionally substituted by one or more halogen atoms; R4 is H, or C1-C8 alkyl optional substituted with one or more halogen; R5a is H, CyCe alkyl optional substituted with one or more halogen, -(C0-C4 alkyl)-C6-C14 aryl or -3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; RY is H, CrC8 alkyl optional substituted with one or more halogen, -(C0-C4alkyl)-C6-C14 aryl or-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; R6 is C-|-C8 alkyl optionally substituted by one or more halogen atoms; C3-C10 cycloalkyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; C.|-C8 alkoxy optionally substituted by one or more halogen atoms; OH; CN; halogen; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R5a and R6 together with the atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; or R5aand R^ together with the atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R11 and R13 are each independently H, C.|-C8 alkyl optionally substituted by one or more halogen atoms, C3-C10 cycloalkyl or -(C.|-C4 alkyl)-C3-C8 cycloalkyl; R12, R14, and R15 are each independently H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C^-CA alkyl-C3-C8 cycloalkyl; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, Oand S, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; or R11 and R12, and R13 and R14 together with the nitrogen atom to which they are attached may form a 4 to 14 membered heterocyclic group optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, benzyl, O-benzyl, C-|-C6 alkyl optionally substituted by one or more OH groups or NH2 groups, C^Cg alkyl optionally substituted by one or more halogen atoms, C^Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21, NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, CN, N02, halogen or a 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, 0 and S; R19and R21 are each independently H; C^Cg alkyl; C3-C8cycloalkyl; C1-C4alkoxy-C1-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, C^Cg alkyl and C(0)C.|-Cg alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, 0 and S, optionally substituted by one or more groups selected from halogen, C^Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, CrC4 alkoxy, C(0)NH2, C(0)NHC.|-Cg alkyl or C(0)N(C.|-Cg alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, O and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C1-C6 alkyl; C^Cg alkyl optionally substituted by one or more halogen atoms; C^Cg alkoxy optionally substituted by one or more OH groups orC1-C4 alkoxy; and C(0)0C.|-Cg alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by C^Cg alkyl, C^Cg haloalkyl or C^Cg alkoxy.

Embodiment 29: The compound according to embodiment 28, wherein A is N or CR4a; X is NRy or O; R1 is CrC4 alkyl optionally substituted by one or more halogen atoms; CrC4 alkoxy optionally substituted by one or more halogen atoms; halogen; -(C0-C4 alkyl)-C6 aryl; or - (C0-C4 alkyl)-5 to 6 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, aryl and heterocyclyl groups are each optionally substituted by one or more Z substituents; R2 is CrC4 haloalkyl; R3 and R4a are H; R4 is H, or CrC4 alkyl optional substituted with one or more halogen; R5a is H, CrC4 alkyl optional substituted with one or more halogen, -(C0-C4 alkyl)-C6 aryl or -5 to 8 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6 aryl and -5 to 8 membered heterocyclic group are each optionally substituted by one or more Z substituents; RY is H, C.1-C4 alkyl optional substituted with one or more halogen, -(C0-C4 alkyl)-C6 aryl or -5 to 8 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6 aryl and -5 to 8 membered heterocyclic group are each optionally substituted by one or more Z substituents; R6 is CrC4 alkyl optionally substituted by one or more halogen atoms; CrC4 alkoxy optionally substituted by one or more halogen atoms; OH; CN; -(C0-C4 alkyl)-C6 aryl; or - 5 to 8 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -C6 aryl and -5 to 8 membered heterocyclic group are each optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R5a and R6 together with the atoms to which they are bound a 5 to 8 membered heterocyclic group containing one or more heteroatoms selected from N, O and S, wherein the heterocyclic group is optionally substituted by one or more Z substituents; or R5a and RY together with the atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, C^Cg alkyl optionally substituted by one or more OH groups or NH2 groups, C^Cg alkyl optionally substituted by one or more halogen atoms, C^Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18C(0)R21, C(0)NR19R21, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, CN, N02, halogen or a 5 to 8 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the heterocyclic group is option substituted by halogen, C.|-C4 alkyl optionally substituted by halogen, C.|-C4 alkoxy or -CN; R18 is H orCrC4 alkyl; R19 and R21 are each independently H; C^Cg alkyl; C3-C8 cycloalkyl; CVC4 alkoxy-C,-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C-|-C6 alkyl, C-|-C6 alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, C^Cg alkyl and C(0)C.|-Cg alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C.|-Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, 0 and S, optionally substituted by one or more groups selected from halogen, C^Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, 0Γ04 alkoxy, C(0)NH2, 0(0)ΝΗ0Γ06 alkyl or C(0)N(CrC6 alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, O and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C1-C6 alkyl; C-|-C6 alkyl optionally substituted by one or more halogen atoms; C-|-C6 alkoxy optionally substituted by one or more OH groups or C.|-C4 alkoxy; and C(0)0C.|-Cg alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by C^Cg alkyl, C^Cg haloalkyl or C^Cg alkoxy.

Embodiment 30: The compound according to embodiment 28 or 29, wherein A is N or CR4a; X is NRYorO; R1 is C-|-C4 alkyl optionally substituted by one or more halogen atoms; C.|-C4 alkoxy optionally substituted by one or more halogen atoms; or halogen; R2 is CF3; R3 and R4a are H; R4 is H, or CrC4 alkyl optional substituted with one or more halogen; R5a is H, CyC4 alkyl optional substituted with one or more halogen, RY is H, CrC4 alkyl optional substituted with one or more halogen, R6 is CrC4 alkyl optionally substituted by one or more halogen atoms; CrC4 alkoxy optionally substituted by one or more halogen atoms; OH ; CN; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 6 membered carbocyclic ring system; or R5a and R6 together with the atoms to which they are bound a 5 to 8 membered heterocyclic group containing one or more heteroatoms selected from N, O and S, wherein the heterocyclic group is optionally substituted by one or more Z substituents; or R5a and RY together with the atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; Z is independently OH, C-|-C6 alkyl optionally substituted by one or more OH groups or NH2 groups, C-|-C6 alkyl optionally substituted by one or more halogen atoms, C^Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, ON, N02, or halogen;

Rt9 is H; C^Cg alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; (C0-C4 alkyl)- 3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, C^Cg alkyl and C(0)C.|-Cg alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, C^Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, CyC4 alkoxy, C(0)NH2, C(0)NHC1-C6 alkyl or C(0)N(C1-C6 alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 6-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, 0 and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C.|-Cg alkyl; C^Cg alkyl optionally substituted by one or more halogen atoms; C^Cg alkoxy optionally substituted by one or more OH groups or C1-C4 alkoxy; and C(0)0C.|-Cg alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by C^Cg alkyl, C^Cg haloalkyl or C^Cg alkoxy.

Embodiment 31 : The compound according to embodiment 28 to 30, wherein A is N or CR4a; X is NRYorO; R1 is C1-C4 alkyl optionally substituted by one or more halogen atoms; C1-C4 alkoxy optionally substituted by one or more halogen atoms; or halogen; R2 is CF3; R3 and R4a are H; R4 is H, or CrC4 alkyl optional substituted with one or more halogen; R5a is H, C.|-C4 alkyl optional substituted with one or more halogen, RY is H, C.|-C4 alkyl optional substituted with one or more halogen, R6 is C^-C4 alkyl optionally substituted by one or more halogen atoms; C^-C4 alkoxy optionally substituted by one or more halogen atoms; OH; CN; or R5a and R6 together with the atoms to which they are bound a 5 to 8 membered heterocyclic group containing one or more heteroatoms selected from N, O and S, wherein the heterocyclic group is optionally substituted by one or more Z substituents; or R5a and RY together with the atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; Z is independently OH, C^Cg alkyl optionally substituted by one or more OH groups or NH2 groups, C^Cg alkyl optionally substituted by one or more halogen atoms, C-|-C6 alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, oxo, CN, N02, or halogen;

Embodiment 32: The compound according to any of the proceeding embodiments, A is N.

Embodiment 33: The compound to embodiments 1 to 31, wherein A is CR4a.

Embodiment 34: The compound according to embodiment 33, wherein A is CR4a and R4a is H.

Embodiment 35: The compound according to any proceeding embodiment, wherein R2 is CF3CF2-, (CF3)2CH-, CH3-CF2-, CF3CF2-, CF3, CF2H-, CH3-CCI2-, CFgCFCCIH-, CBr3, CBr2H-CF3CF2CHCF3 or CF3CF2CF2CF2-.

Embodiment 36: The compound according to any proceeding embodiment, wherein R2 is CF3.

Embodiment 37: The compound according to any proceeding embodiment, wherein the compound is a substantially pure enantiomer with the S configuration.

Embodiment 38: The compound according to embodiment 1 to 36, wherein the compound is a substantially pure enantiomer with the R configuration.

Embodiment 39: The compound according to embodiment 2, 21 or 28, wherein the compound is represented by: 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro -2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [(R)-1-(tetrahydrofuran-2-yl)methyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ([1,3]dioxolan-2-ylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [(S)-1-(tetrahydrofuran-2-yl)methyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (tetrahydro-furan-2-ylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methyl-2-piperidin-1-yl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methyl-tetrahydro-furan-2-yl-methyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methoxy-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [2-(4-fluorophenyl)-2-morpholin-4-yl-ethyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-morpholin-4-yl-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-dimethylamino-2-phenyl-ethyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-chloro-2-methyl-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hy-droxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-trifluoromethyl-pro-pyl)-amide; 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide; 3-amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-me-thyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide; 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-trifluorome-thyl-propyl)-amide; 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro- pyl)-amide; 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro- pyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide;

Methyl 3-(3-amino-6-bromo-5-(trifluoromethyl)picolinamido)propanoate; 3-Amino-N-(benzo[d]isoxazol-3-ylmethyl)-6-bromo-5-(trifluoromethyl) picolinamide; 3-Amino-6-(oxazol-2-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-6-bromo-N-(3,3,3-trifluoro-2-methoxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-amino-N-(2-hydroxy-3-methyl-2-(trifluoromethyl)butyl)-6-methoxy-5-(trifluoromethyl)picolinamide; 3-Amino-6-cyclopropyl-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-hydroxy-2-(trifluoromethyl)propyl)-5-(trifluoro methyl) picolinamide; 5-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-3-(trifluoromethyl)-2,4’-bipyridine-6-carboxamide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3-methyl-2-oxobutyl)-amide; 3-Amino-6-(1-methyl-1 H-pyrazol-4-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolina-mide; (S)-3-amino-6-ethoxy-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoro methyl)picolinamide; 3-Amino-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-N-(2-amino-3,3,3-trifluoro-2-methylpropyl)-6-methoxy-5-(trifluoromethyl) picolinamide; or 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methylpropyl)-5-(trifluoromethyl)picolina-mide.

Embodiment 40: The compound according to embodiment 39, wherein the compound is 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [(R)-1-(tetrahydrofuran-2-yl)methyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ([1,3]dioxolan-2-ylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [(S)-1-(tetrahydrofuran-2-yl)methyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (tetrahydro-furan-2-ylmethyl)-amide; or 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methyltetrahydro-furan-2-yl-methyl)-amide.

Embodiment 41 : The compound according to embodiment 2, 21 or 28, wherein the compound is 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-fluorophenyl)-2-morpholin-4-yl-ethyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [2-(4-fluorophenyl)-2-morpholin-4-yl-ethyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-morpholin-4-yl-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-dimethylamino-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (3-methyl-2-morpholin-4-yl-butyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-methyl-2-morpholin-4-yl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (1-morpholin-4-ylcyclohexylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-morpholin-4-yl-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-dimethylamino-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-methoxy-phenyl)-2-pyrrolidin-1-yl-ethyl]- amide; 3-Amino-N-(2-amino-3,3,3-trifluoro-2-methylpropyl)-6-methoxy-5-(trifluoromethyl) picolinamide; or 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-dimethylamino-2-(4-methoxy-phenyl)-ethyl]- amide.

Embodiment 42: The compound according to embodiment 2, 21 or 28, wherein the compound is 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-methyl-tetrahydro-furan-2-yl-methyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-fluoro-phenyl)-2-morpholin-4-yl-ethyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (3-methyl-2-morpholin-4-yl-butyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-methyl-2-morpholin-4-yl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (1-morpholin-4-yl-cyclohexylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-morpholin-4-yl-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid (2-dimethylamino-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-methoxy-phenyl)-2-pyrrolidin-1-yl-ethyl]- amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-dimethylamino-2-(4-methoxy-phenyl)-ethyl]- amide; 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-fluoro-phenyl)-2-oxo-ethyl]-amide; 3-Amino-6-furan-2-yl-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(2-methoxyphenyl)-ethyl]-amide; 3-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5,6-bis(trifluoromethyl) pyrazine-2-carboxamide; N-(2-(1H-imidazol-2-yl)propyl)-3-amino-6-bromo-5-(trifluoromethyl)pyrazine-2-carboxamide; 3-Amino-6-bromo-N-(2-morpholinoethyl)-5-(trifluoromethyl)pyrazine-2-carboxamide; or 3-Amino-6-bromo-5-trifluoromethyl-pyrazine-2-carboxylic acid [2-(4-fluoro-phenyl)-2-oxo-ethyl]-amide.

Embodiment 43: The compound according to embodiment 39, wherein the compound is 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-propyl)-amide; 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide; or 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide.

Embodiment 44: Use of a compound according to embodiment 1 to 43 in the manufacture of a medicament for use in the treatment of an inflammatory or obstructive airways disease or mucosal hydration.

Embodiment 45: Use of a compound according to embodiment 1 to 43 in the manufacture of a medicament for use in the treatment of a disease mediated by CFTR.

Embodiment 46: Use of a compound according to embodiment 42 in the manufacture of a medicament for use in the treatment of a disease mediated by CFTR, wherein the disease is CF or COPD.

Embodiment 47: Use of a compound according to embodiment 1 to 43 in the manufacture of a medicament for use in the treatment of cystic fibrosis.

Embodiment 48: A pharmaceutical composition for treating a disease or disorder mediated by CFTR, comprising: the compound according to embodiment 1 to 43 and one or more pharmaceutically acceptable excipients.

Embodiment 49: A pharmaceutical composition, according to embodiment 48, wherein the disease or disorder is cystic fibrosis or COPD.

Embodiment 49: A pharmaceutical composition, according to embodiment 49, wherein the disease or disorder is cystic fibrosis.

Embodiment 50: A pharmaceutical combination, comprising: a first active comprising the compound according to embodiment 1 to 43 and a second active selected from osmotic agents, ENaC blockers, anti-inflammatory agents, bronchodilatory agents, antihistamine agents, anti-tussive agents, antibiotic agents and DNase drug substances, wherein the first and second actives may be in the same or different pharmaceutical composition.

Embodiment 51: A pharmaceutical combination according to embodiment 50, wherein the second active agent is an EnaC blocker.

Embodiment 52: A process for the preparation of compounds of formula (I), comprising:

I reacting a compound 1 with compound 2 in a peptide coupling reaction,

1 2 wherein R1, R2, R3, R4, R5 and R6 are as defined herein and P is a suitable amino protecting group; removing protecting groups and isolating the compound of formula I.

Embodiment 53: The process according to embodiment 48, wherein the peptide coupling condition is HATU in an aprotic solvent.

Claims

1. A compound of Formula I

or pharmaceutically acceptable salts thereof, wherein: A is N or CR4a; R1 is H; C-|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; C1-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; S02NR8R9; S02R10; S-C1-C8alkyl optionally substituted by one or more halogen atoms; S-C6-C14 aryl; CN; NR11R12; C(0)NR13R14; NR13S02R15 ; NR13C(0)R15, Cr02R15, -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclic groups are each optionally substituted by one or more Z substituents; R2 is C-|-C4 haloalkyl; R3 and R4a are each independently H or C1-C8 alkyl optionally substituted by one or more halogen atoms; R4 is H, or C.|-C8 alkyl optional substituted with one or more halogen; R5 is-(CH2)m-NR17R18, -(CH2)m-OR; C.|-C8 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C02R15 -(C0-C4 alkyl)-C6-C14 aryl or -3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; R6 is C-|-C8 alkyl optionally substituted by one or more halogen atoms; C3-C10 cycloalkyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; C.|-C8 alkoxy optionally substituted by one or more halogen atoms; OH; CN; halogen; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; wherein the cycloalkyl, cycloalkenyl, -(C0-C4 alkyl)-C6-C14 aryl and -(C0-C4 alkyl)-3 to 14 membered heterocyclic group are each optionally substituted by one or more Z substituents; or R6 is H, and R5 is -(CH2)m-NR17R18, -(CH2)m-OR, C^Cg alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; or -(C0-C4 alkyl)-C02R15, wherein -(C0-C4 alkyl)-C6-C14aryl and -(C0-C4alkyl)-3 to 14 membered heterocyclic group groups are each optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 8 membered carbocyclic ring system; or R4 and R5 togetherform an oxo group (C=0) and R6 is C1-C4 alkyl optionally substituted by one or more halogen atoms; C.|-C4 alkoxy optionally substituted by one or more halogen atoms; -(C0-C4 alkyl)-C6-C14 aryl; or-(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents; or R5 and R6 together with the carbon atoms to which they are bound a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; or R4 and R5 and R6 together with the carbon atoms to which they are bound form a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; R is H, or C^Cg alkyl optional substituted with one or more halogen; m is 0, 1,2 or 3; R8, R11, R13 and R17 are each independently H, C.|-C8 alkyl optionally substituted by one or more halogen atoms, C3-C-10 cycloalkyl or-(C-|-C4 alkyl)-C3-C8 cycloalkyl; R9, R10, R12, R14, R15 and R18 are each independently H; C.|-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclic groups are each optionally substituted by one or more Z substituents; or R8 and R9, R11 and R12, R13 and R14, and R17 and R18 together with the nitrogen atom to which they are attached may form a 4 to 14 membered heterocyclic group optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, benzyl, O-benzyl, 0-,-Cg alkyl optionally substituted by one or more OH groups or NH2 groups, 0.,-Cg alkyl optionally substituted by one or more halogen atoms, 0.,-Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21 NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, ON, no2, halogen or a 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; R19and R21 are each independently H; C.|-C8 alkyl; C3-C8cycloalkyl; C1-C4alkoxy-C1-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C.,-C6 alkyl, 0.,-Cg alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, 0-,-Cg alkyl and C(0)C^-C& alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from 0-,-Cg alkyl, 0-,-Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, 0.,-Cg alkyl or C(0)C.|-Cg alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, CrC4 alkoxy, C(0)NH2, 0(0)ΝΗ0Γ06 alkyl or 0(0)Ν(0Γ06 alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, O and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C.|-Cg alkyl; 0.,-Cg alkyl optionally substituted by one or more halogen atoms; 0.,-Cg alkoxy optionally substituted by one or more OH groups orC1-C4 alkoxy; and 0(0)00.,-C6 alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by 0-,-Cg alkyl, 0-,-Cg haloalkyl or 0-,-Cg alkoxy. 2. The compound according to claim 1, wherein A is CR4a. 3. The compound according to claim 1 or claim 2, wherein R1 is C-|-C8 alkyl optionally substituted by one or more halogen atoms; C-|-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; NR11R12, C6-C14 aryl; or-(C0-C4 alkyl)-5 to 6 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents. 4. The compound according to any preceding claim, wherein R4 is H or CrC4 alkyl optionally substituted by one or more halogen atoms; R5 is C-|-C4 alkoxy optionally substituted by one or more halogen atoms; -(CH2)m-NR17R18, -(CH2)m-OR ; or-(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the heterocyclic groups is optionally substituted by one or more Z substituents; R6 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; C.|-C4 alkoxy optionally substituted by one or more halogen atoms; or -(C0-C4 alkyl)-C6-C14 aryl wherein the aryl is optionally substituted by one or more Z substituents; or R4 and R6 together with the carbon atoms to which they are bound form a 3 to 6 membered carbocyclic ring system; or R5 and R6 together with the carbon atoms to which they are bound form a 5 to 8 membered heterocyclic ring system containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted by one or more Z substituents; m is 0 or 1 ; R17 and R18 are each independently H; C1-C8 alkyl optionally substituted by one or more halogen atoms. 5. The compound according to any of the preceding claims, wherein A is CR4a; R1 is C.|-C4 alkyl optionally substituted by one or more halogen atoms; or C^-C4 alkoxy optionally substituted by one or more halogen atoms; R2 is CF3; R3 is H, CH3 or CF3; R4 is H or Me; R4a is H; R5 is -NR17R18 or OH, and R6 is C^-C4 alkyl optionally substituted by one or more halogen atoms. 6. A compound represented by formula II,

or a pharmaceutically acceptable salt; wherein A is N or CR4a; R4a is FI or C.|-C4 alkyl; R1 is C.|-C8 alkyl optionally substituted by one or more halogen atoms; C.|-C8 alkoxy optionally substituted by one or more halogen atoms; halogen; NR11R12, C6-C14 aryl; or -(C0-C4 alkyl)-5 to 6 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein the aryl and heterocyclic groups are each optionally substituted by one or more Z substituents; R3 is FI or CFI3; R101 is

R11 is H, C-|-C8 alkyl optionally substituted by one or more halogen atoms, C3-C10 cycloalkyl or -(C.|-C4 alkyl)-C3-C8 cycloalkyl; R12 and R18 are each independently H; C1-C8 alkyl optionally substituted by one or more halogen atoms; C2-C8 alkenyl; C2-C8 alkynyl; C3-C10 cycloalkyl; C5-C10 cycloalkenyl; -C.|-C4 alkyl-C3-C8 cycloalkyl; -(C0-C4 alkyl)-C6-C14 aryl; or -(C0-C4 alkyl)-3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at leastone heteroatom selected from N, O and S, wherein the cycloalkyl, cycloalkenyl, aryl and heterocyclic groups are each optionally substituted by one or more Z substituents; or R11 and R12 together with the nitrogen atom to which they are attached may form a 4 to 14 mem bered heterocyclic group optionally substituted by one or more Z substituents; Z is independently OH, aryl, O-aryl, benzyl, O-benzyl, C^Cg alkyl optionally substituted by one or more OH groups or NH2 groups, C^Cg alkyl optionally substituted by one or more halogen atoms, C^Cg alkoxy optionally substituted by one or more OH groups or CrC4 alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21 NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, oxo, CN, N02, halogen or a 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S; R19and R21 are each independently H; C.,-C8 alkyl; C3-C8cycloalkyl; C1-C4alkoxy-C1-C4 alkyl; (C0-C4 alkyl)-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; (C0-C4 alkyl)-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, oxo, C^Cg alkyl and 0(0)0-|-06 alkyl; (C0-C4 alkyl)-0-aryl optionally substituted by one or more groups selected from C^Cg alkyl, C^Cg alkoxy and halogen; and (C0-C4 alkyl)- 0-3- to 14-membered heterocyclic group, the heterocyclic group including one or more heteroatoms selected from N, O and S, optionally substituted by one or more groups selected from halogen, C^Cg alkyl or 0(0)0-|-06 alkyl; wherein the alkyl groups are optionally substituted by one or more halogen atoms, CrC4 alkoxy, C(0)NH2, 0(0)ΝΗ0.|-06 alkyl or 0(0)Ν(0-|-06 alkyl)2; or R19 and R21 together with the nitrogen atom to which they attached form a 5- to 10-membered heterocyclic group, the heterocyclic group including one or more further heteroatoms selected from N, O and S, the heterocyclic group being optionally substituted by one or more substituents selected from OH; halogen; aryl; 5- to 10-membered heterocyclic group including one or more heteroatoms selected from N, O and S; S(0)2-aryl; S(0)2-C1-C6 alkyl; C^Cg alkyl optionally substituted by one or more halogen atoms; C^Cg alkoxy optionally substituted by one or more OH groups orC1-C4 alkoxy; and 0(0)00-|-06 alkyl, wherein the aryl and heterocyclic substituent groups are themselves optionally substituted by C^Cg alkyl, C^Cg haloalkyl or C^Cg alkoxy. 7. The compound according to claim 1, selected from 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [(R)-1-(tetrahydro-furan-2-yl)methyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ([1,3]dioxolan-2-ylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [(S)-1-(tetrahydro-furan-2-yl)methyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (tetrahydro-furan-2-ylmethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methyl-2-piperidin-1-yl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methyl-tetrahydrofuran-2-yl-methyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-methoxy-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid [2-(4-fluoro-phenyl)-2-morpholin-4-yl-ethyl]-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-morpholin-4-yl-2-phenyl-ethyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (2-dimethylamino-2-phenyl-ethyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-chloro-2-methyl-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-trifluoromethyl-propyl)-amide; 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-amino-6-(2,4-dichloro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-propyl)-amide; 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-trifluoromethyl-propyl)-amide; 5-Amino-6’-methyl-3-trifluoromethyl-[2,3’]bipyridinyl-6-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide; 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide;

Methyl 3-(3-amino-6-bromo-5-(trifluoromethyl)picolinamido)propanoate; 3-Amino-6-(oxazol-2-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-6-bromo-N-(3,3,3-trifluoro-2-methoxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-amino-N-(2-hydroxy-3-methyl-2-(trifluoromethyl)butyl)-6-methoxy-5-(trifluoromethyl)picolinamide; 3-Amino-6-cyclopropyl-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-hydroxy-2-(trifluoromethyl)propyl)-5-(trifluoro methyl) picolinamide; 5-amino-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-3-(trifluoromethyl)-2,4’-bipyridine-6-carboxamide; 3-Amino-6-bromo-5-trifluoromethyl-pyridine-2-carboxylic acid (3-methyl-2-oxo-butyl)-amide; 3-Amino-6-(1-methyl-1H-pyrazol-4-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; (S)-3-amino-6-ethoxy-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoro methyl)picolinamide; 3-Amino-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-methylpropyl)-5-(trifluoromethyl)picolinamide; 3-Amino-N-(2-amino-3,3,3-trifluoro-2-methylpropyl)-6-methoxy-5-(trifluoromethyl) picolinamide; and 3-Amino-6-methoxy-N-(3,3,3-trifluoro-2-(4-methoxybenzylamino)-2-methylpropyl)-5-(trifluoromethyl)picolinamide; or a pharmaceutically acceptable salt thereof. 8. The compound according to claim 7, which is: 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide, or a pharmaceutically acceptable salt thereof. 9. The compound according to claim 7, which is: 3-Amino-6-methoxy-5-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-pro-pyl)-amide, or a pharmaceutically acceptable salt thereof. 10. The compound according to claim 7, which is: 3-Amino-6-(4-fluoro-phenyl)-5-trifluoromethyl-pyridine-2-carboxylic acid (3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)-amide, or a pharmaceutically acceptable salt thereof. 11. The compound according to claim 7, which is: 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((S)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)- amide; or a pharmaceutically acceptable salt thereof. 12. The compound according to claim 7, which is: 3-Amino-5,6-bis-trifluoromethyl-pyridine-2-carboxylic acid ((R)-3,3,3-trifluoro-2-hydroxy-2-methyl-propyl)- amide, or a pharmaceutically acceptable salt thereof. 13. A compound, or a pharmaceutically acceptable salt thereof, according to any of Claims 1 to 12 for use as a pharmaceutical. 14. A compound, ora pharmaceutically acceptable salt thereof, according to any of Claims 1 to 12 for use in the treatment of an inflammatory or obstructive airways disease or conditions benefiting from mucosal hydration. 15. A compound, ora pharmaceutically acceptable salt thereof, according to any of Claims 1 to 12 for use in the treatment of cystic fibrosis. 16. Use of a compound, or a pharmaceutically acceptable salt thereof, according to any of Claims 1 to 12 in the manufacture of a medicament for use in the treatment of an inflammatory or obstructive airways disease or conditions benefiting from mucosal hydration. 17. A pharmaceutical composition, comprising: the compound, or a pharmaceutically acceptable salt thereof, according to any of Claims 1 to 12 and one or more pharmaceutically acceptable excipients. 18. A pharmaceutical combination, comprising: a first active comprising the compound, ora pharmaceutically acceptable salt thereof, according to any of Claims 1 to 12 and a second active selected from osmotic agents, ENaC blockers, anti-inflammatory agents, bronchodilatory agents, antihistamine agents, anti-tussive agents, antibiotic agents and DNase drug substances, wherein the first and second actives may be in the same or different pharmaceutical composition.

Patentansprüche

1. Verbindung der Formel I

I oder pharmazeutisch annehmbare Salze davon, worin: A für N oder CR4a steht; R1 für Folgendes steht: H ; C.|-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C2-C8-Alkenyl; C2-C8-Al kinyi; C3-C10-Cycloalkyl; C5-C10-Cycloalkenyl; -C1-C4-Alkyl-C3-C8-Cycloalkyl; C^C^AIkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; Halogen; S02NR8R9; S02R10; S-C1-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; S-C6-C14-Aryl; CN; NR11R12; C(0)NR13R14; NR13S02R15; NR13C(0)R15, C02R15, -(C0-C4-Alkyl)-C6-C14-aryl; odereine3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom aufweist, das ausgewählt ist aus N, O und S; wobei die Cycloalkyl-, Cycloalkenyl-, Aryl- und heterocyclischen Gruppen jeweils mit einem oder mehreren Z-Substituenten substituiert sind; R2 für C-|-C4-Haloalkyl steht; R3 und R4a jeweils unabhängig für H oder C.|-C8-Alkyl stehen, das optional mit einem oder mehreren Haloge-natom(en) substituiert ist; R4 für H oder C1-C8-Alkyl steht, das optional mit einem oder mehreren Halogen(en) substituiert ist; R5 für Folgendes steht: -(CH2)m-NR17R18, -(CH2)m-OR’; C.|-C8-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; -(C0-C4-Alkyl)-CO2R15; - (C0-C4-Alkyl)-C6-C14-aryl odereine 3- bis 14-gliedrige heterocyclische Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S; wobei das -(C0-C4-Alkyl)-C6-C14-aryl und die 3- bis 14-gliedrige heterocyclische (C0-C4-Al-kyl)-Gruppe jeweils optional mit einem oder mehreren Z-Substituenten substituiert ist; R6 für Folgendes steht: C.|-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C3-C10-Cycloalkyl; -C.|-C4-Alkyl-C3-C8-cycloalkyl; C^C^AIkoxy, das optional mit einem oder mehreren Halo-genatom(en) substituiert ist; OH; CN; Halogen; -(C0-C4-Alkyl)-C6-C14-aryl; oder eine 3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S; wobei das Cycloalkyl, das Cycloalkenyl, das -(C0-C4-Alkyl)-C6-C14-aryl und die 3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe jeweils optional mit einem oder mehreren Z-Substi-tuenten substituiert sind; oder R6 für H steht, und R5 für Folgendes steht: -(CH2)m-NR17R18, -(CH2)m-OR; C.|-C8-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; -(C0-C4-Alkyl)-C6-C14-Aryl; eine 3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S; oder-(C0-C4-Alkyl)-CO2R15, wobei das -(C0-C4-Alkyl)-C6-C14-aryl und die 3- bis 14-gliedrige heterocyclische (C0-C4-Alkyl)-Gruppe jeweils optional mit einem oder mehreren Z-Substitu-enten substituiert sind; oder R4 und R6 zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, ein 3-bis 8-gliedriges carbocyclisches Ringsystem bilden; oder R4 und R5 zusammen eine Oxogruppe (C=0) bilden und R6 für Folgendes steht: C.|-C4-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; CrC4-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; - (C0-C4-Alkyl)-C6-C14-aryl; oder eine 3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S, wobei die Aryl- und heterocyclischen Gruppen jeweils optional mit einem oder mehreren Z-Substituenten substituiert sind; oder R5 und R6 zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, ein 5-bis 8-gliedriges heterocyclisches Ringsystem bilden, das ein oder mehrere Heteroatom(e) enthält, das/die ausgewählt ist/sind aus N, O und S, wobei das Ringsystem optional mit einem oder mehreren Z-Substituenten substituiert ist; oder R4 und R5 und R6 zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, ein 5- bis 8-gliedriges heterocyclisches Ringsystem bilden, das ein oder mehrere Heteroatom(e) enthält, das/die ausgewählt ist/sind aus N, O und S, wobei das Ringsystem optional mit einem oder mehreren Z-Substituenten substituiert ist; R’ für H oder C.|-C8-Alkyl steht, das optional mit einem oder mehreren Halogenen substituiert ist; m für 0, 1,2 oder 3 steht; R8, R11, R13 und R17 jeweils unabhängig für Folgendes stehen: H, C-]-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist, C3-C10-Cycloalkyl oder -(C1-C4-Alkyl)-C3-C8-cycloalkyl; R9, R10, R12, R14, R15 und R18jeweils unabhängig für Folgendes stehen: H; C.|-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C2-C8-Alkenyl; C2-C8-Alkinyl; C3-C10-Cycloalkyl; C5-C10-Cy-cloalkenyl; -C1-C4-Alkyl-C3-C8-cycloalkyl; -(C0-C4-Alkyl)-C6-C14-aryl; odereine 3- bis 14-gliedrige heterocyclische - (C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthalt, das ausgewählt ist aus N, O und S, wobei die Cycloalkyl-, Cycloalkenyl-, Aryl- und heterocyclischen Gruppen jeweils optional mit einem oder mehreren Z-Substituenten substituiert sind; oder R8 und R9, R11 und R12, R13 und R14 und R17 und R18 zusammen mit dem Stickstoffatom, an dem sie hängen, eine 4- bis 14-gliedrige heterocyclische Gruppe bilden können, die optional mit einem oder mehreren Z-Sub-stituenten substituiert ist; Z unabhängig für Folgendes steht: OH, Aryl, O-Aryl, Benzyl, O-Benzyl, C-|-Cg-Alkyl, das optional mit einer oder mehreren OH-Gruppe(n) oder NH2-Gruppe(n) substituiert ist, C^Cg-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist, C^Cg-Alkoxy, das optional mit einer oder mehreren OH-Gruppe(n) substituiert ist, oder C1-C4-Alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21, NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, Oxo, CN, N02, Halogen oder eine 3- bis 14-gliedrige heterocyclische Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S; R19 und R21 jeweils unabhängig für Folgendes stehen: H; C1-C8-Alkyl; C3-C8-Cycloalkyl; C^C^AIkoxy-C^C^al-kyl; (C0-C4-Alkyl)-aryl, das optional substituiert ist mit einer oder mehreren Gruppe(n), die ausgewählt sind aus C^Cg-Alkyl, C^Cg-Alkoxy und Halogen; eine 3- bis 14-gliedrige heterocyclische (C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe ein oder mehrere Heteroatom(e) enthält, das/die ausgewählt ist/sind aus N, O und S, optional substituiert mit einer oder mehreren Gruppe(n),die ausgewählt ist/sind aus Halogen, Oxo, C^Cg-Alkyl und C(0)C-|-Cg-Alkyl; (C0-C4-Alkyl)-O-aryl, das optional substituiert ist mit einer oder mehreren Gruppe(n), die ausgewählt sind aus C-|-Cg-Alkyl, C^Cg-Alkoxy und Halogen; und eine 0-3- bis 14-gliedrige heterocyclische (C0-C4-Alkyl)-O-Gruppe, wobei die heterocyclische Gruppe ein oder mehrere Heteroatom(e) enthält, das/die ausgewählt ist/sind aus N, O und S, die optional substituiert ist mit einer oder mehreren Gruppe(n), die ausgewählt ist/sind aus Halogen, C^Cg-Alkyl oder C(0)C.|-Cg-Alkyl; wobei die Alkylgruppen optional mit einem oder mehreren Halogenatom(en), C^C^AIkoxy, C(0)NH2, C(0)NHC.|-Cg-Alkyl oder C(0)N(C1-C6-Alkyi)2 substituiert sind; oder R19 und R21 zusammen mit dem Stickstoffatom, an dem sie hängen, eine 5- bis 10-gliedrige heterocyclische Gruppe bilden, wobei die heterocyclische Gruppe ein oder mehrere weitere(s) Heteroatom(e) aufweist, das/die ausgewählt ist/sind aus N, O und S, wobei die heterocyclische Gruppe optional mit einem oder mehreren Substituenten substituiert ist, der/die ausgewählt ist/sind aus OH; Halogen; Aryl; einer 5- bis 10-gliedrigen heterocyclischen Gruppe, die ein oder mehrere Heteroatom(e) aufweist, das/die ausgewählt ist/sind aus N, O und S; S(0)2-Aryl; S(0)2-C-|-Cg-Alkyl; C-|-Cg-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C^Cg-Alkoxy, das optional substituiert ist mit einer oder mehreren OH-Gruppe(n) oder C.|-C4-Alk-oxy; und C(0)0C.|-Cg-Alkyl, wobei die Aryl- und heterocyclischen Substituentengruppen ihrerseits optional mit C^Cg-Alkyl, C^Cg-Haloalkyl oder C^Cg-Alkoxy substituiert sind. 2. Verbindung nach Anspruch 1, wobei A für CR4a steht. 3. Verbindung nach Anspruch 1 oder Anspruch 2, wobei R1 für Folgendes steht: C^Cg-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C^Cg-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; Halogen; NR11R12, C6-C14-Aryl; odereine 5- bis 6-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S, wobei die Aryl- und heterocyclischen Gruppen jeweils optional mit einem oder mehreren Z-Substituenten substituiert sind. 4. Verbindung nach einem vorhergehenden Anspruch, wobei R4 für H oder C.|-C4-Alkyl steht, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; R5 für Folgendes steht: C.|-C4-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; -(CH2)m-NR17R18, -(CH2)m-OR’; oder eine 3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S, wobei die heterocyclische Gruppe optional mit einem oder mehreren Z-Substituenten substituiert ist; R6 für Folgendes steht: C.|-C4-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C.|-C4-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; oder -(C0-C4-Al-kyl)-C6-C14-aryl, wobei das Aryl optional mit einem oder mehreren Z-Substituenten substituiert ist; oder R4 und R6 zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, ein 3- bis 6-gliedriges carbocyclisches Ringsystem bilden; oder R5 und R6 zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, ein 5- bis 8-gliedriges heterocyclisches Ringsystem bilden, das ein oder mehrere Heteroatom(e) enthält, das/die ausgewählt ist/sind aus N, O und S, wobei das Ringsystem optional mit einem oder mehreren Z-Substituenten substituiert ist; m für 0 oder 1 steht; R17 und R18 jeweils unabhängig für H oder C^Cg-Alkyl stehen, das optional mit einem oder mehreren Halogena-tom(en) substituiert ist. 5. Verbindung nach einem der vorhergehenden Ansprüche, wobei A für CR4a steht; R1 für Folgendes steht: C-|-C4-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; oder C-|-C4-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; R2 für CF3 steht; R3 für H, Chi3 oder CF3 steht; R4 für Fl oder Me steht; R4a für H steht; R5 für -NR17R18 oder OH steht, und R6 für C1-C4-Alkyl steht, das optional mit einem oder mehreren Halogenatom(en) substituiert ist. 6. Verbindung, dargestellt durch die Formel II,

oder ein pharmazeutisch annehmbares Salz; worin A für N oder CR4a steht; R4a für H oder C.|-C4-Alkyl steht; R1 für Folgendes steht: C1-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C1-C8-Alkoxy, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; Halogen; NR11R12, C6-C14-Aryl; odereine 5- bis 6-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S, wobei die Aryl- und heterocyclischen Gruppen jeweils optional mit einem oder mehreren Z-Substituenten substituiert sind; R3 für H oder CH3 steht; R101 für Folgendes steht:

oder ; R11 für Folgendes steht: H, C.|-C8-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist, C3-C10-Cycloalkyl oder-(C1-C4-Alkyl)-C3-C8-cycloalkyl; R12 und R18 jeweils unabhängig für Folgendes stehen: H; C^Cg-Alkyl, das optional mit einem oder mehreren Flalogenatom(en) substituiert ist; C2-C8-Alkenyl; C2-C8-Alkinyl; C3-C10-Cycloalkyl; C5-C10-Cycloalkenyl; -C^C^Alkyl-C^Cg-cycloalkyl; -(C0-C4-Alkyl)-C6-C14-aryl; oder eine 3- bis 14-gliedrige heterocyclische -(C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe mindestens ein Fleteroatom enthalt, das ausgewählt ist aus N, O und S, wobei die Cycloalkyl-, Cycloalkenyl-, Aryl- und heterocyclischen Gruppen jeweils optional mit einem oder mehreren Z-Substituenten substituiert sind; oder R11 und R12 zusammen mit dem Stickstoffatom, an dem sie hängen, eine 4- bis 14-gliedrige heterocyclische Gruppe bilden können, die optional mit einem oder mehreren Z-Substituenten substituiert ist; Z unabhängig für Folgendes steht: Ohl, Aryl, O-Aryl, Benzyl, O-Benzyl, C^Cg-Alkyl, das optional mit einer oder mehreren OFI-Gruppe(n) oder NFI2-Gruppe(n) substituiert ist, C^Cg-Alkyl, das optional mit einem oder mehreren Flalogenatom(en) substituiert ist, C^Cg-Alkoxy, das optional mit einer oder mehreren OFI-Gruppe(n) substituiert ist, oder C1-C4-Alkoxy, NR18(S02)R21, (S02)NR19R21, (S02)R21, NR18C(0)R21, C(0)NR19R21, NR18C(0)NR19R21, NR18C(0)0R19, NR19R21, C(0)0R19, C(0)R19, SR19, OR19, Oxo, CN, N02, Halogen oder eine 3- bis 14-gliedrige heterocyclische Gruppe, wobei die heterocyclische Gruppe mindestens ein Heteroatom enthält, das ausgewählt ist aus N, O und S; R19 und R21 jeweils unabhängig für Folgendes stehen: H; C^Cg-Alkyl; C3-C8-Cycloalkyl; C^C^AIkoxy-C^C^al-kyl; (C0-C4-Alkyl)-aryl, das optional substituiert ist mit einer oder mehreren Gruppe(n), die ausgewählt ist/sind aus C^Cg-Alkyl, C^Cg-Alkoxy und Halogen; eine 3- bis 14-gliedrige heterocyclische (C0-C4-Alkyl)-Gruppe, wobei die heterocyclische Gruppe ein oder mehrere Heteroatom(e) enthält, das/die ausgewählt ist/sind aus N, O und S, die optional substituiert ist mit einer oder mehreren Gruppe(n), die ausgewählt ist/sind aus Halogen, Oxo, C^Cg-Alkyl und CfOjC^Cg-Alkyl; (C0-C4-Alkyl)-O-aryl, das optional substituiert ist mit einer oder mehreren Gruppe(n), die ausgewählt ist/sind aus C^Cg-Alkyl, C^Cg-Alkoxy und Halogen; und eine 3- bis 14-gliedrige heterocyclische (C0-C4-Alkyl)-O-Gruppe, wobei die heterocyclische Gruppe ein oder mehrere Heteroatom(e) aufweist, das/die ausgewählt ist/sind aus N, O und S, die optional substituiert mit einer oder mehreren Gruppe(n), die ausgewählt ist/sind aus Halogen, C^Cg-Alkyl oder C(0)C1-C6-Alkyi; wobei die Alkylgruppen optional mit einem oder mehreren Halogenatom(en), C1-C4-Alkoxy, C(0)NH2, CfOjNHC^Cg-Alkyl oder C(0)N(C.|-Cg-Al-kyl)2 substituiert sind; oder R19 und R21 zusammen mit dem Stickstoffatom, an dem sie hängen, eine 5- bis 10-gliedrige heterocyclische Gruppe bilden, wobei die heterocyclische Gruppe ein oder mehrere weitere(s) Heteroatom(e) aufweisen, das/die ausgewählt ist/sind aus N, O und S, wobei die heterocyclische Gruppe optional mit einem oder mehreren Substituenten substituiert ist, der/die ausgewählt ist/sind aus OH; Halogen; Aryl; einer 5- bis 10-gliedrigen heterocyclischen Gruppe, die ein oder mehrere Heteroatom(e) aufweist, das/die ausgewählt ist/sind aus N, O und S; S(0)2-Aryl; S(0)2-C.|-Cg-Alkyl; C^Cg-Alkyl, das optional mit einem oder mehreren Halogenatom(en) substituiert ist; C^Cg-Alkoxy, dasoptional miteinerodermehreren OH-Gruppe(n) substituiert istoderC^C^AIk-oxy; und C(0)OC1-C6-Alkyl, wobei die Aryl- und heterocyclischen Substituentengruppen ihrerseits optional mit C^Cg-Alkyl, C^Cg-Haloalkyl oder C^Cg-Alkoxy substituiert sind. 7. Verbindung nach Anspruch 1, ausgewählt aus 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxypropyl)amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-((S)-3,3,3-trifluor-2-hydroxypropyl)amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-[(R)-1-(tetrahydrofuran-2-yl)methyl]-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-([1,3]dioxolan-2-ylmethyl)]-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-[(S)-1-(tetrahydrofuran-2-yl)methyl]-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(tetrahydrofuran-2-ylmethyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-methyl-2-piperidin-1-yl-propyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-hydroxypropyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-methyltetrahydrofuran-2-yl-methyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-methoxyethyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-[2-(4-fluorphenyl-2-morpholin-4-ethyl]-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-morpholin-4-yl-2-phenylethyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(2-dimethylamino-2-phenylethyl)-amid; 3-Amino-6-(4-fluorphenyl)-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-(4-chlor-2-methylphenyl)-5-trifluormethylpyridin-2-carbonsäure-((R)-3,3,3-trifluor-2-hydroxypro- pyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-trifluormethylpropyl)-amid; 5-Amino-6’-methyl-3-trifluormethyl-[2,3’]-bipyridinyl-6-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-((R)-3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-((S)-3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-methoxy-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-(4-fluorphenyl)-5-trifluormethylpyridin-2-carbonsäure-((S)-3,3,3-trifluor-2-hydroxy-2-methylpro- pyl)-amid; 3-Amino-6-(4-fluorphenyl)-5-trifluormethylpyridin-2-carbonsäure-((R)-3,3,3-trifluor-2-hydroxy-2-methylpro- pyl)-amid; 3-Amino-6-(2,4-dichlorphenyl)-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpro- pyl)-amid; 3-Amino-6-(4-fluorphenyl)-5-trifluormethylpyridin-2-carbonsäure-(2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-methoxy-5-trifluormethylpyridin-2-carbonsäure-(3,3,3-trifluor-2-hydroxypropyl)-amid; 5-Amino-6’-methyl-3-trifluormethyl-[2,3’]-bipyridinyl-6-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-trifluormethylpro- pyl)-amid; 5-Amino-6’-methyl-3-trifluormethyl-[2,3’]bipyridinyl-6-carbonsäure-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)]-amid; 3-Amino-5,6-bis-trifluormethylpyridin-2-carbonsäure-((S)-3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-5,6-bis-trifluormethylpyridin-2-carbonsäure-((R)-3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-methoxy-5-trifluormethylpyridin-2-carbonsäure-((S)-3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid; 3-Amino-6-methoxy-5-trifluormethylpyridin-2-carbonsäure-((R)-3,3,3-trifluor-2-hydroxy-2-methylpropyl)-amid;

Methyl-3-(3-amino-6-brom-5-(trifluormethyl)-picolinamido)-propanoat; 3-Amino-6-(oxazol-2-yl)-N-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-5-(trifluormethyl)-picolinamid; 3-Amino-6-brom-N-(3,3,3-trifluor-2-methoxy-2-methylpropyl)-5-(trifluormethyl)-picolinamid; 3-Amino-N-(2-hydroxy-3-methyl-2-(trifluormethyl)-butyl)-6-methoxy-5-(trifluormethyl)-picolinamid; 3-Amino-6-cyclopropyl-N-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-5-(trifluormethyl)-picolinamid; 3-Amino-6-methoxy-N-(3,3,3-trifluor-2-hydroxy-2-(trifluormethyl)-propyl)-5-(trifluormethyl)-picolinamid; 5-Amino-N-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-3-(trifluormethyl)-2,4’-bipyridin-6-carboxamid; 3-Amino-6-brom-5-trifluormethylpyridin-2-carbonsäure-(3-methyl-2-oxobutyl)-amid; 3-Amino-6-(1-methyl-1H-pyrazol-4-yl)-N-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-5-(trifluormethyl)-picolinamid; (S)-3-Amino-6-ethoxy-N-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-5-(trifluormethyl)-picolinamid; 3-Amino-6-(pyrolidin-1-yl)-N-(3,3,3-trifluor-2-hydroxy-2-methylpropyl)-5-(trifluormethyl)-picolinamid; 3-Amino-N-(2-amino-3,3,3-trifluor-2-methylpropyl)-6-methoxy-5-(trifluoromethyl)-picolinamid; und 3-Amino-6-methoxy-N-(3,3,3-trifluor-2-(4-methoxybenzylamino)-2-methylpropyl)-5-(trifluormethyl)-picolinamid; odereinem pharmazeutisch annehmbaren Salz davon. 8. Verbindung nach Anspruch 7, bei der es sich um Folgendes handelt: 3-Amino-6-methoxy-5-trifluormethylpyridin-2-carbonsäure ((S)-3,3,3-Trifluor-2-hydroxy-2-methylpropyl)-amid oder ein pharmazeutisch annehmbares Salz davon. 9. Verbindung nach Anspruch 7, bei der es sich um Folgendes handelt: 3-Amino-6-methoxy-5-trifluormethylpyridin-2-carbonsäure ((R)-3,3,3-Trifluor-2-hydroxy-2-methylpropyl)-amid oder ein pharmazeutisch annehmbares Salz davon. 10. Verbindung nach Anspruch 7, bei der es sich um Folgendes handelt: 3-Amino-6-(4-fluorphenyl)-5-trifluormethylpyridin-2-carbonsäure (3,3,3-Trifluor-2-hydroxy-2-methylpro- pyl)-amid oder ein pharmazeutisch annehmbares Salz davon. 11. Verbindung nach Anspruch 7, bei der es sich um Folgendes handelt: 3-Amino-5,6-bis-trifluormethylpyridin-2-carbonsäure ((S)-3,3,3-Trifluor-2-hydroxy-2-methylpropyl)-amid oder ein pharmazeutisch annehmbares Salz davon. 12. Verbindung nach Anspruch 7, bei der es sich um Folgendes handelt: 3-Amino-5,6-bis-5-trifluormethylpyridin-2-carbonsäure ((R)-(3,3,3-Trifluor-2-hydroxy-2-methylpropyl)-amid oder ein pharmazeutisch annehmbares Salz davon. 13. Verbindung oder pharmazeutisch annehmbares Salz davon nach einem der Ansprüche 1 bis 12 zur Verwendung als Pharmazeutikum. 14. Verbindung oder pharmazeutisch annehmbares Salz davon nach einem der Ansprüche 1 bis 12 zur Verwendung bei der Behandlung einer/eines entzündlichen oder obstruktiven Atemwegserkrankung oder-zustands, auf die/den sich Schleimhauthydratation positiv auswirkt. 15. Verbindung oder pharmazeutisch annehmbares Salz davon nach einem der Ansprüche 1 bis 12 zur Verwendung bei der Behandlung von zystischer Fibrose. 16. Verwendung einer Verbindung oder eines pharmazeutisch annehmbaren Salzes davon nach einem der Ansprüche 1 bis 12 bei der Herstellung eines Medikaments zur Verwendung bei der Behandlung einer/eines entzündlichen oder obstruktiven Atemwegserkrankung oder-zustands, auf die/den sich Schleimhauthydratation positiv auswirkt. 17. Pharmazeutische Zusammensetzung, umfassend: die Verbindung oderein pharmazeutisch annehmbares Salz davon nach einem der Ansprüche 1 bis 12 und einen oder mehrere pharmazeutisch annehmbare Hilfsstoffe. 18. Pharmazeutische Zusammensetzung, die Folgendes umfasst: einen ersten Wirkstoff, der die Verbindung oder ein pharmazeutisch annehmbares Salz davon nach einem der Ansprüche 1 bis 12 umfasst, und einen zweiten Wirkstoff, der ausgewählt ist aus Osmosemitteln, ENaC-Blockern, entzündungshemmenden Mitteln, bronchodilatatorischen Mitteln, Antihistaminmitteln, Antihustenmitteln, Antibiotika und DNase-Arznei-mittelsubstanzen, wobei der erste und zweite Wirkstoff in der gleichen oder einer anderen pharmazeutischen Zusammensetzung vorliegen können.

Revendications 1. Composé de formule I :

I ou l’un de ses sels pharmaceutiquement acceptable, dans lequel : A représente N ou CR4a ; R1 représente un H; un C.|-C8 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C2-C8 alcényle ; un C2-C8alcynyle ; un C3-C10 cycloalkyl ; unC5-C10cycloalcényle ; un-C1-C4alkyl-C3-C8cycloalkyle ; un C.|-C8alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un halogène ; un S02NR8R9 ; un S02R1° ; un S-C^Cg alkyl facultativement substitué par un ou plusieurs atomes d’halogène ; un S-C6-C14 aryle; un CN ; un NR11R12 ; un C(0)NR13R14 ; un NR13S02R15 ; un NR13C(0)R15, un C02R15, un -(C0-C4 alkyl)-C6-C14 aryle ; ou groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S ; où le cycloalkyle, le cycloalcényle, l’aryle et les groupes hétérocycliques sont facultativement substitués par un ou plusieurs substituants Z ; R2 représente un C.|-C4 haloalkyle ; R3 et R4a représentent chacun indépendamment un H ou un C^Cg alkyle facultativement substitués par un ou plusieurs atomes d’halogène ; R4 représente un H, ou un C^Cg alkyle facultativement substitué par un ou plusieurs halogènes ; R5 représente un -(CH2)m-NR17R18, un -(CH2)m-OR’; un C^Cg alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un - (C0-C4 alkyl)-C02R15 ; un -(C0-C4 alkyl)-C6-C14 aryle ou un groupe hétérocyclique à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S; où le -(C0-C4 alkyl)-C6-C14 aryle et le groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons sont chacun facultativement substitués par un ou plusieurs substituants Z ; R6 représente un C^Cg facultativement substitué par un ou plusieurs atomes d’halogène ; un C3-C10 cycloalkyle ; un CrC4 alkyl-C3-C8 cycloalkyle ; un C^Cg alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un OH ; un CN; un halogène ; un -(C0-C4 alkyl)-C6-C14 aryle ; ou un groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S; où le cycloalkyle, le cycloalcényle, le -(C0-C4 alkyl)-C6-C14 aryle et le groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons sont chacun facultativement substitués par un ou plusieurs substituants Z ; ou R6 représente un H, et R5 représente un - (CH2)m-NR17R18, un-(CH2)m-OR’, un C^Cg alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un -(C0-C4 alkyl)-C6-C14 aryle ; un groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S; ou le -(C0-C4 alkyl)-C02R15, où le -(C0-C4 alkyl)-C6-C14 aryle et le groupe hétérocyclique à 3 à 14 chaînons sont chacun facultativement substitués par un ou plusieurs substituants Z ; ou R4 et R6 ensemble avec les atomes de carbone auxquels ils sont liés forment un système d’anneau carbocyclique à 3 à 8 chaînons ; ou R4 et R5 ensemble forment un groupe oxo (C=0) et R6 représente un CrC4 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C.|-C4 alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un -(C0-C4 alkyl)-C6-C14 aryle ; ou un groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S , où l’aryle et les groupes hétérocycliques sont chacun facultativement substitués par un ou plusieurs substituants Z ; ou R5 et R6 ensemble avec les atomes de carbone auxquels ils sont liés forment un système d’anneau hétérocyclique à 5 à 8 chaînons, contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S ; où le système d’anneau est facultativement substitué par un ou plusieurs substituants Z ; ou R4 et R5 et R6 ensemble avec les atomes de carbone auxquels ils sont liés forment un système d’anneau hétérocyclique à 5 à 8 chaînons, contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, où le système d’anneau est facultativement substitué par un ou plusieurs substituants Z ; R’ représente un H, ou un C^Cg alkyle facultativement substitué par un ou plusieurs halogènes ; m est égal à 0, 1,2 ou 3 ; R8, R11, R13 et R17 sont chacun indépendamment un H, un C^Cg alkyle facultativement substitués par un ou plusieurs atomes d’halogène, un C3-C10 cycloalkyle ou un -(C.|-C4 alkyl)-C3-C8 cycloalkyle ; R9, R10, R12, R14, R15 et R18 sont chacun indépendamment un H ; un C^Cg alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C2-C8 alcényle ; un C2-C8 alcynyle ; un C3-C10 cycloalkyl ; un C5-C10 cycloalcényle ; un -CrC14 alkyl-C3-C8 cycloalkyl ; un -(C0-C4 alkyl)-C6-C14 aryle ; ou un groupe hétérocyclique -(C0-C4 alkyl)- à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S, où le cycloalkyle, le cycloalkenyle, l’aryle et les groupes hétérocycliques sont chacun facultativement substitués par un ou plusieurs substituants Z ; ou R8 et R9, R11 et R12, R13 et R14, et R17 et R18 ensemble avec l’atome d’azote auquel ils sont liés peuventformer un groupe hétérocyclique à 4 à 14 chaînons facultativement substitués par un ou plusieurs substituants Z ; Z représente indépendamment un OH, un aryle, un O-aryle, un benzyle, un O-benzyle, un C^Cg alkyle facultativement substitué par un ou plusieurs groupes OH ou groupes NH2, un C^Cg alkyle facultativement substitué par un ou plusieurs atomes d’halogène, un C^Cg alkoxy facultativement substitué par un ou plusieurs groupes OH ou un CrC4 alkoxy, un NR18(S02)R21, un (S02)NR19R21, un (S02)R21, un NR18C(0)R21, un C(0)NR19R21, un NR18C(0)NR19R21, un NR18C(0)0R19, un NR19R21, un C(0)0R19, un C(0)R19, un SR19, un OR19, unoxo, un CN, un N02, un halogène ou un groupe hétérocyclique à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O ou un S ; R19 et R21 sont chacun indépendamment un H ; un C^Cg alkyle ; un C3-C8 cycloalkyle ; un C.|-C4 alkoxy-C.|-C4 alkyle ; un (C0-C4 alkyl)-aryle facultativement substitué par un ou plusieurs groupes choisis parmi un C^Cg alkyle, un C^Cg alkoxy et un halogène ; un groupe hétérocyclique (C0-C4 alkyl)- à 3 à 14-chaînons, le groupe hétérocyclique contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, facultativement substitué par un ou plusieurs groupes choisis parmi un halogène, un oxo, un C^Cg alkyle et un C(0)C.|-Cg alkyle ; un (C0-C4 alkyl)-0-aryle facultativement substitué par un ou plusieurs groupes choisis parmi un C^Cg alkyle, un C^Cg alkoxy et un halogène ; et un groupe hétérocyclique (C0-C4 alkyl)- O- à 3 à 14-chaînons, le groupe hétérocyclique contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, facultativement substitué par un ou plusieurs groupes choisis parmi un halogène, un C-|-C6 alkyle et un C(0)Ci-C6 alkyle ; où les groupes alkyles sont facultativement substitués par un ou plusieurs atomes d’halogène, un CrC4 alkoxy, un C(0)NH2, un C(0)NHC.|-Cg alkyle ou un C(0)N(C.|-Cg alkyl)2 ; ou R19 et R21 ensemble avec l’atome d’azote auquel ils sont liés forment un groupe hétérocyclique à 5 à 10 chaînons, le groupe hétérocyclique comprenant en outre un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, le groupe hétérocyclique étant facultativement substitué par un ou plusieurs substituants choisis parmi un OH ; un halogène ; un aryle ; un groupe hétérocyclique à 5 à 10 chaînons, comprenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S ; un S(0)2-aryle ; un S(0)2-C.|-Cgalkyle ; un C^Cg alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C^Cg alkoxy facultativement substitué par un ou plusieurs groupes OH ou un C1-C4 alkoxy ; et un C(0)0C.|-Cg alkyle, où l’aryle et les groupes hétérocycliques substituants sont eux-mêmes facultativement substitués par un C^Cg alkyle, un C^Cg haloalk-yle ou un C^Cg alkoxy. 2. Composé selon la revendication 1, dans lequel A représente CR4a. 3. Composé selon la revendication 1 ou la revendication 2, dans lequel R1 représente un C^Cg alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C^Cg alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un halogène ; un NR11R12, un C6-C14 aryle ; ou un groupe hétérocyclique (C0-C4 alkyl)- à 5 à 6 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S, où l’aryle et les groupes hétérocycliques sont chacun facultativement substitués par un ou plusieurs substituants Z. 4. Composé selon l’une quelconque des revendications précédentes, dans lequel R4 représente un H, ou un CrC4 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; R5 représente un C-|-C4 alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un -(CH2)m-NR17R18, un -(CH2)m-OR’ ; ou un groupe hétérocyclique -(C0-C4 alkyl)-à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S, où les groupes hétérocycliques sont facultativement substitués par un ou plusieurs substituants Z ; R6 représente un C1-C4 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C1-C4 alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; ou un -(C0-C4 alkyl)-C6-C14 aryle dans lequel l’aryle est facultativement substitué par un ou plusieurs substituants Z ; ou R4 et R6 ensemble avec les atomes de carbone auxquels ils sont liés forment un système d’anneau carbocyclique à 3 à 6 chaînons ; ou R5 et R6 ensemble avec les atomes de carbone auxquels ils sont liés forment un système d’anneau hétérocyclique à 5 à 8 chaînons, contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S ; où le système d’anneau est facultativement substitué par un ou plusieurs substituants Z ; m est égal à 0 ou 1 ; R17 et R18 représentent chacun indépendamment un H ; un C-pCg alkyle facultativement substitué par un ou plusieurs atomes d’halogène. 5. Composé selon l’une quelconque des revendications précédentes, dans lequel A représente CR4a ; R1 représente un C1-C4 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; ou un CrC4 alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; R2 représente un CF3 ; R3 représente un H, un CH3 ou un CF3 ; R4 représente un H ou un Me ; R4a représente un H ; R5 représente un -NR17R18 ou un OH, et R6 représente un C.|-C4 alkyle facultativement substitué par un ou plusieurs atomes d’halogène. 6. Composé représenté par la formule II,

ou l’un de ses sels pharmaceutiquement acceptable ; dans lequel A représente N ou CR4a ; R4a représente un H ou un C.|-C4 alkyle ; R1 représente un C.|-C8 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C1-C3 alkoxy facultativement substitué par un ou plusieurs atomes d’halogène ; un halogène ; un NR11 R12, un C6'C14 aryle ; ou un groupe hétérocyclique -(C0-C4 alkyl)- à 5 à 6 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S, où l’aryle et les groupes hétérocycliques sont chacun facultativement substitués par un ou plusieurs substituants Z ; R3 représente un H ou un CH3 ; R101 représente

R11 représente un H, un C.|-C8 alkyle facultativement substitué par un ou plusieurs atomes d’halogène, un C3-C10 cycloalkyle ou un -(C.|-C4 alkyl)-C3-C8 cycloalkyle ; R12 et R18 sont chacun indépendamment un H ; un 0Γ08 alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C2-C8 alcényle ; un C2-C8 alcynyle ; un C3-C10 cycloalkyle ; un C5-C10 cycloalkenyle; un -C.|-C4 alkyl-C3-C8 cycloalkyle ; un -(C0-C4 alkyl)-C6-C14 aryle ; ou un groupe hétérocyclique -(C0-C4 alkyl)-à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O et un S, où le cycloalkyle, le cycloalcényle, l’aryle et les groupes hétérocycliques sont chacun facultativement substitués par un ou plusieurs substituants Z ; ou R11 et R12 ensemble avec l’atome d’azote auquel ils sont liés peuvent former un groupe hétérocyclique à 4 à 14 chaînons facultativement substitué par un ou plusieurs substituants Z ; Z représente indépendamment un OH, un aryle, un O-aryle, un benzyle, un O-benzyle, un C^Cg alkyle facultativement substitués par un ou plusieurs groupes OH ou groupes NH2, un C^Cg alkyle facultativement substitué par un ou plusieurs atomes d’halogène, un C^Cg alkoxy facultativement substitué par un ou plusieurs groupes OH ou un CrC4 alkoxy, un NR18(S02)R21, un (S02)NR19R21, un (S02)R21, un NR18C(0)R21, un C(0)NR19R21, un NR18C(0)NR19R21, un NR18C(0)0R19, un NR19R21, un C(0)0R19, un C(0)R19, un SR19, un OR19, unoxo, un CN, un N02, un halogène ou un groupe hétérocyclique à 3 à 14 chaînons, où le groupe hétérocyclique contient au moins un hétéroatome choisi parmi un N, un O ou un S ; R19 et R21 sont chacun indépendamment un H ; un C^Cg alkyle ; un C3-C8 cycloalkyle ; un C1-C4 alkoxy-C1-C4 alkyle ; un (C0-C4 alkyl)-aryle facultativement substitué par un ou plusieurs groupes choisis parmi un C^Cg alkyle, un C^Cg alkoxy et un halogène ; un groupe hétérocyclique (C0-C4 alkyl)- à 3 à 14 chaînons, le groupe hétérocyclique contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, facultativement substitué par un ou plusieurs groupes choisis parmi un halogène, un oxo, un C^Cg alkyle et un C(0)C.|-Cg alkyle ; un (C0-C4 alkyi)-0-aryle facultativement substitué par un ou plusieurs groupes choisis parmi un C^Cg alkyle, un C^Cg alkoxy et un halogène ; et un groupe hétérocyclique (C0-C4 alkyl)- O- à 3 à 14-chaînons, le groupe hétérocyclique contenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, facultativement substitué par un ou plusieurs groupes choisis parmi un halogène, un C^Cg alkyle et un C(0)C.|-C6 alkyle ; où les groupes alkyles sont facultativement substitués par un ou plusieurs atomes d’halogène, un C-|-C4 alkoxy, un C(0)NH2, un C(0)NHC.|-C6 alkyle ou un C(0)N(C.|-C6 alkyl)2 ; ou R19 et R21 ensemble avec l’atome d’azote auquel ils sont liés forment un groupe hétérocyclique à 5 à 10 chaînons, le groupe hétérocyclique comprenant en outre un ou plusieurs hétéroatomes choisis parmi un N, un O et un S, le groupe hétérocyclique étant facultativement substitué par un ou plusieurs atomes choisis parmi un OH ; un halogène ; un aryle ; un groupe hétérocyclique à 5 à 10 chaînons, comprenant un ou plusieurs hétéroatomes choisis parmi un N, un O et un S ; un S(0)2-aryle ; un S(0)2-C.|-C6alkyle ; un C^Cg alkyle facultativement substitué par un ou plusieurs atomes d’halogène ; un C^Cg alkoxy facultativement substitué par un ou plusieurs groupes OH ou un C.1-C4 alkoxy ; et un C(0)0C.|-Cg alkyle, où l’aryle et les groupes hétérocycliques substituants sont eux-mêmes facultativement substitués par un C^Cg alkyle, un C^Cg haloalk-yle ou un C^Cg alkoxy. 7. Composé selon la revendication 1, choisi parmi 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro -2-hydroxy-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique ((S)-3,3,3-trifluoro-2-hydroxy-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique [(R)-1-(tetrahydro-furan-2-yl)méthyl]-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique ([1,3]dioxolan-2-ylméthyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique [(S)-1-(tetrahydro-furan-2-yl)méthyl]-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (tetrahydro-furan-2-ylméthyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-méthyl-2-piperidin-1-yl-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-hydroxy-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-méthyl-tetrahydro-furan-2-yl-méthyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-méthoxy-éthyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique [2-(4-fluoro-phényl)-2-morpholin-4-yl-éthyl]- amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-morpholin-4-yl-2-phényl-éthyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (2-diméthylamino-2-phényl-éthyl)-amide ; 3-Amino-6-(4-fluoro-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-(4-chloro-2-méthyl-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique ((R)-3,3,3-trifluoro-2-hy-droxy-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-trifluorométhyl- propyl)-amide ; 5-Amino-6’-méthyl-3-trifluorométhyl-[2,3’]bipyridinyl-6-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique ((R)-3,3,3-trifluoro-2-hydroxy-2-méthyl- propyl)-amide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique ((S)-3,3,3-trifluoro-2-hydroxy-2-méthyl- propyl)-amide ; 3-Amino-6-méthoxy-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-(4-fluoro-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique ((S)-3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-(4-fluoro-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique ((R)-3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-amino-6-(2,4-dichloro-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-(4-fluoro-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique (2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-6-méthoxy-5-trifluorométhyl-pyridine-2-acide carboxilique (3,3,3-trifluoro-2-hydroxy-propyl)-amide ; 5-Amino-6’-méthyl-3-trifluorométhyl-[2,3’]bipyridinyl-6-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-trifluoromé-thyl-propyl)-amide ; 5-Amino-6’-méthyl-3-trifluorométhyl-[2,3’]bipyridinyl-6-acide carboxilique (3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; 3-Amino-5,6-bis-trifluorométhyl-pyridine-2-acide carboxilique ((S)-3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)- amide ; 3-Amino-5,6-bis-trifluorométhyl-pyridine-2-acide carboxilique ((R)-3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-ami-de; 3-Amino-5,6-bis-trifluorométhyl-pyridine-2-acide carboxilique ((S)-3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)- amide ; 3-Amino-6-méthoxy-5-trifluorométhyl-pyridine-2-acide carboxilique ((R)-3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ; méthyl 3-(3-amino-6-bromo-5-(trifluorométhyl)picolinamido)propanoate ; 3-Amino-6-(oxazol-2-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-méthylpropyl)-5-(trifluorométhyl)picolinamide ; 3-Amino-6-bromo-N-(3,3,3-trifluoro-2-méthoxy-2-méthylpropyl)-5-(trifluorométhyl)picolinamide ; 3-amino-N-(2-hydroxy-3-méthyl-2-(trifluorométhyl)butyl)-6-méthoxy-5-(trifluorométhyl)picolinamide ; 3-Amino-6-cyclopropyl-N-(3,3,3-trifluoro-2-hydroxy-2-méthylpropyl)-5-(trifluorométhyl)picolinamide ; 3-Amino-6-méthoxy-N-(3,3,3-trifluoro-2-hydroxy-2-(trifluorométhyl)propyl)-5-(trifluoro méthyl) picolinamide ; 5-amino-N-(3,3,3-trifluoro-2-hydroxy-2-méthylpropyl)-3-(trifluorométhyl)-2,4’-bipyridine-6-carboxamide ; 3-Amino-6-bromo-5-trifluorométhyl-pyridine-2-acide carboxilique (3-méthyl-2-oxo-butyl)-amide ; 3-Amino-6-(1-méthyl-1H-pyrazol-4-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-méthylpropyl)-5-(trifluorométhyl)picolinamide ; (S)-3-amino-6-éthoxy-N-(3,3,3-trifluoro-2-hydroxy-2-méthylpropyl)-5-(trifluoro méthyl)picolinamide ; 3-Amino-6-(pyrrolidin-1-yl)-N-(3,3,3-trifluoro-2-hydroxy-2-méthylpropyl)-5-(trifluorométhyl)picolinamide ; 3-Amino-N-(2-amino-3,3,3-trifluoro-2-méthylpropyl)-6-méthoxy-5-(trifluorométhyl) picolinamide ; et 3-Amino-6-méthoxy-N-(3,3,3-trifluoro-2-(4-méthoxybenzylamino)-2-méthylpropyl)-5-(trifluorométhyl)picolinamide ; ou un sel pharmaceutiquement acceptable de celui-ci. 8. Composé selon la revendication 7, qui est : 3-Amino-6-méthoxy-5-trifluorométhyl-pyridine-2-acide carboxilique((S)-3,3,3-trifluoro-2-hydroxy-2-méthyl-pro-pyl)-amide ou un sel pharmaceutiquement acceptable de celui-ci. 9. Composé selon la revendication 7, qui est : 3-Amino-6-méthoxy-5-trifluorométhyl-pyridine-2-acide carboxilique((R)-3,3,3-trifluoro-2-hydroxy-2-méthyl-pro-pyl)-amide ou un sel pharmaceutiquement acceptable de celui-ci. 10. Composé selon la revendication 7, qui est : 3-Amino-6-(4-fluoro-phényl)-5-trifluorométhyl-pyridine-2-acide carboxilique(3,3,3-trifluoro-2-hydroxy-2-méthyl-propyl)-amide ou un sel pharmaceutiquement acceptable de celui-ci. 11. Composé selon la revendication 7, qui est : 3-Amino-5,6-bis-trifluorométhyl-pyridine-2-acide carboxilique((S)-3,3,3-trifluoro-2-hydroxy-2-méthyl-pro- pyl)-amide ou un sel pharmaceutiquement acceptable de celui-ci. 12. Composé selon la revendication 7, qui est : 3-Amino-5,6-bis-trifluorométhyl-pyridine-2-acide carboxilique((R)-3,3,3-trifluoro-2-hydroxy-2-méthyl-pro- pyl)-amide ou un sel pharmaceutiquement acceptable de celui-ci. 13. Composé ou sel pharmaceutiquement acceptable de celui-ci selon l’une quelconque des revendications 1 à 12 pour une utilisation comme produit pharmaceutique. 14. Composé ou sel pharmaceutiquement acceptable de celui-ci selon l’une quelconque des revendications 1 à 12 pour une utilisation dans le traitement des maladies inflammatoires ou ventilatoires obstructives ou des troubles améliorés par une hydratation des muqueuses. 15. Composé ou sel pharmaceutiquement acceptable de celui-ci selon l’une quelconque des revendications 1 à 12 pour une utilisation dans le traitement de la fibrose cystique. 16. Utilisation d’un composé ou d’un sel pharmaceutiquement acceptable de celui-ci selon l’une quelconque des revendications 1 à 12 dans la fabrication d’un médicament à utiliser dans le traitement des maladies inflammatoires ou ventilatoires obstructives, ou des troubles améliorés par une hydratation des muqueuses. 17. Composition pharmaceutique comprenant: un composé ou un sel pharmaceutiquement acceptable de celui-ci selon l’une quelconque des revendications 1 à 12, et un ou plusieurs excipients pharmaceutiquement acceptables. 18. Combinaison pharmaceutique comprenant: un premier actif comprenant le composé ou un sel pharmaceutiquement acceptable de celui-ci selon l’une quelconque des revendications 1 à 12, et un deuxième actif choisi parmi des agents osmotiques, des bloquants ENaC, des agents anti-inflammatoires, des agents broncho-dilatatoires, des agents antihistaminiques, des agents antitussifs, des agents antibiotiques et des substances médicamenteuses DNase, où le premier et le deuxième actifs peuvent avoir la même composition pharmaceutique ou une composition pharmaceutique différente.

Claims (9)

Pyridine and pyrazine derivatives for treating F F claims 1, I: wherein: A is h1 or CR4a: R1 is H; optionally one or more halogen atoms! substituted (1-8 slides · atoms) -alk1i; (C2-C8 alkenyl; (C2-C8) alkyl: nll; (C3-C18)} · Cycloalkyl; (C5-C10) -c} Cl: lke? Vi! 1-4 carbonatones} -alkyl- (C3-C8) -cycloalkyl, optionally substituted with one or more halogen atoms (C1-C8) alkoxy, halogenCom; optionally: one or more halogen substituted S- (C8-C8 alkyl; 3- (6-14 carbon atoms; N; NRUR.3 · 2; C (0) MR! ïRî4; 'NRn $ û> RÎ3; NRi3C (G) RiS, CO2Ris, - ((C4-C4) -alkyl) - (6-14 carbon atoms) or - ((0-4 carbon atoms) os} ·· is 1 ki I) - 3 to 14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N. 0 and S, wherein each of cloalkyl, cycloalkenyl, aryl and heterocyclic is optionally present substituted with 2 sttensensesyls; R2 is (C1-C4-haloalkyl; R3 and R4a are each independently H or specific); t (X-C8) substituted with one or more halogen atoms; R4 is H or optionally substituted with one or more halogen atoms (1-8 atoms) - pyl; R 5 is -CH 2 --NR 1 R 8 '-, - (CH a jm-OR; optionally one or more balogens are substituted today (1-8 carbon atoms - ((0-4 Senate) -alkyl) -CCnR 1:> ; - ((C 0 -C 4) -alkyl) (ii) - (C 6 -C 14 -aryl or -3-14-membered heterocyclic group, wherein the heterocyclic group contains at least one of the atoms selected from Ni, ö and S; - ((0 to 4 carbon atoms) - ai: kll) - (C 6 to C 14) -an 1 - and ((C 4 -C 4) -alkyl) - 3 · t to 3, 4-membered heterocyclic group optionally have one or more 2 substituted with a substituent · R * optionally represents one or more halogen atoms &lt; RTI ID = 0.0 &gt; (&lt; / RTI &gt; C8-C8) -alkyl; (3-H) carbon &quot;ciyoalki]; - (C1-C8) -cycloalkyl; in the particular case of one or more halogen-substituted (1-8-serum) alcohols; OH; N; halogen; - {(C4-C4) -OH) - (6) Or (- (4-carbonyl) acyl) - 3- to 14-membered heterocycle where the heterocyclic group contains at least one hotaro atom selected from N, Ö and S; wherein each of cloalalkyl, dioalkenyl, ((0-4 carbon atoms) 05) -alkyl, {6-14 Senate} -aryl, and - ((C 0 -C 4) -alkyl) -3-I, 4-membered heterocyclic group is optionally present substituted with one or more 2 substituents, or R6 is H, and R5 is - {CH2) w -NRx ,, RiS, - (€ H2) m-ORf optionally substituted with one or more halogen atoms (C1-C8) · ' alkoxl; - ((C 0 -C 4) -alkyl) (C 6 -C 14) -aryl; - ((0-4 Senate) -8 kyl) -3-14 membered heterocyclic group, wherein the heterocyclic group is at least one, The heteroatom selected from N, O and S is cleaved; or - ((0-4 alkane-γ-alkyl-CO-jR1- wherein - (0-4 carbon atoms) - ai; Cl:) - (6- Each of the 14 carbon atoms and (- (C 0 -C 4) -alkyl) -3-14-membered heterocyclic groups is optionally substituted with one or more 2 substituents, or R 4 and R * are attached to them with a carbon atom in a 3-8 membered carbocyclic ring or R 4 and taken together form oxo (OQ), and R 0 is optionally substituted with one or more halogen atoms (C 1 -C 4 alkyl optionally substituted by one or more halogen atoms) (C 1 -C 4) alkoxy ; - ((0-4-membered) -alkyl) - (C 6-14) -an or - ({0-4 -halo) -alkyl) -3-14 membered heterocyclic group, wherein the heterocyclic group is at least one, N , HE s contain heteroatoms selected from S, wherein the shl and heterocyclic group may each be optionally substituted with one or more Z substituents; or R * and R &amp; together with the carbon atom to which they are attached, form a 5-8 membered heterocyclic ring member containing one or more heteroatoms selected from N, O and S, wherein the ring system is optionally substituted with one or more Z substituents; or R4 and R5 and R5 together with the carbon atom to which they are attached form a 5-8 membered heterocyclic ring moiety containing one or more heteroatoms selected from the group consisting of ik, 0 and S, wherein the ring system is optionally substituted with one or more 2 substituents; R is H or optionally substituted with one or more halogen atoms (1-8 carbon atoms); m is 0, 1, 2 or 3; Rs, Kn, Ru and Rw are each independently H, optionally substituted with one or more halogen atoms (C1-8 carbon atoms, (3-1.0 carbon atoms, alkyl or - 1 to 4 carbon atoms). .ii :) :-( C3-C8 C1-C10alkyl; each of R5, R6, R14, R14, R5 and R5 is independently H; optionally substituted with one or more halogen atoms (C 1-8); (2-8 slurry) alkynyl; (3-10 carbon atoms) -cycloalkyl; '(5-carbon atoms) -c; 3-S Senate Hdkloalkyl; - ((C 0 -C 4) alkyl) - (6-14 carbon atoms, H 1; or - ((0-4 S 2ene: atamos) -alkyl) -3-C 4 heterocyclic: Aboi the heterocyclic group contains at least one heteroatom selected from H, O and S, wherein each of cycloalkyl, dcloalkenyl, aryl and heterocyclic groups is optionally substituted by one or more substituents Z; or R% and R * 5, f * u, and R 2, Ru and RM; s R / and R 28 together with the nitrogen atom an optionally by one or more szufesztltuált 4-14 Advantage, two substituents may form a heterocyclic group; Z is independently OH, aryl. O-arH, benzyl, benzene, optionally substituted with one or more OH or NHj groups (1-6C) alkyl, optionally substituted with one or more six atom (s), (i-6 carbon atoms, optionally one or more) more OH groups or (C 1 -C 4 alkoxy) (C 1-6 alkoxy; N'R 18 {S? R} R 21, (Scy -NR 4 R 21, (SO: yl) R 8i NRiSC (O) R ;; % C (O) NRî: sRss:;, NR ^NHJOJMR ^R * *, HRCC (O) 0R1§, NR1SR21, CCOjOR 5, C ()): R;, SRiS, ORïS, ovo, CU, UO% bafogen or a 3- to 14-membered heterocyclic group, the heterocyclic group contains at least one of the atoms selected from N, O and S, each of R 1 and R 11 is independently H; Cj4;; ;atatatatatatatatatatatat 4 4 4 4 4 4 4 4 4 4 4 4 4lili;; esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetben esetbenkokokokokokokokokokokokokokokokokokoko, selected substituent ((C 0 -C 4) - alkyl (alkenyl) -f (α-4-carbon atoms) -3-β-membered heterocyclic group, wherein the heterocyclic group contains one or more, selected from the group consisting of M, O and S, and optionally one or more, halogen, 0X0, (C1-C6 alkyl and C (O) (substituted with 8 to 8 carbon atoms); optionally, one or more (1-6 $) -alkyl, (C1-C6-alkoxy and -baiogen-substituted substituents ((0-4 carbon atoms); C3-C4-alkyl} - 0-3-14-membered heterocyclic group, wherein the heterocyclic group contains one or more hsteres selected from Μ, δ and S, and optionally one or more, atoms, (1-6 salts) or (0) (C 1 -C 6 -alkyl; selected substituent; wherein the alkyl groups are optionally one or more halogen, (C 1-4 C-N-alkoxy, C (E) N: H C (O) HH (: C 6 -C 6 alkyl or C (O) IM (C 1 -C 6) alpha) can be substituted with either R 15 and the nitrogen atom together with a nitrogen atom. a heterocyclic group, the heterocyclic group contains one or more further heteroatoms selected from the group consisting of Ny and S, and the heterocyclic group may optionally be one or more of the heteroatoms selected; more; ON; halogen; aryl; one or more heteroatoms selected from the group consisting of M, O and S; S (O) raft; S (O) r (C 1-6 alpha-alkyl; optionally substituted with one or more halogen atoms (1-6)), optionally substituted with eoy or more, OH or (1-4); cyanobacterial afkoxiesopottia) (substituted with 1-6 carbon atoms; and € (0) 0 (1-6 Senate-alpha) is a substituent selected from the group consisting of aryl and heterocyclic substituents themselves, optionally (1- C 6 -alkyl, (1-6 s2eno), haloalkyl, or (1-6C) alkoxy, substituted with groups, 2 * The compound of Claim i wherein A is CR4; A compound according to claim 1 or 2, wherein R 1 is optionally substituted with one or more halogen atoms (C 1 -C 8 alkyl, optionally substituted with one or more halogenated radicals (C 1 -C 8) -alkoxy). HR: uR 2, (C 6-14 aryl; or - (C 0 -C 4) alkyl) -5-6 membered heterocyclic group, wherein the heterocyclic group is selected from at least one of N, O and S wherein each of the aryl and heterocyclyl groups is optionally substituted with one or more Z substituents according to any one of the preceding claims, wherein fc * is H or optionally substituted by one or more halogen atoms (C 1 -C 4 alkyl) ; - optionally substituted with one or more halogen atoms (C 1-4 carbon atoms; - (H 2) m-OR; or - ((0-4 S) atoms): k 1) ~ 3-i 4; heterodicus group, wherein the heterodic group comprises at least one, N, O, and S optionally containing a heteroatom selected, the heterodic groups optionally substituted with one or more Z substituents; R6 is optionally substituted with one or more halogen atoms (i-4 carbon atoms); optionally substituted with one or more halogen atoms (C 1 -C 4) or - ((0-4 S) -alkyl) - (1-4-sera) aryl, where the group is optionally one or more Z or R4 and Rô together with the carbon atom to which they are attached form a 3-6 membered carbodic ring system, or R ~ and R &amp; together with the carbon atom to which they are attached are an S-8 member, one or more of hl, O and $ a heterodic kneading system comprising a selected heteroatom, wherein the ring ester is optionally substituted by one or more Z substituents, m is 0 or 1, each of R1 'and Rss is independently H, optionally substituted with one or more halogen atoms. ai out Compounds according to any one of the preceding claims wherein A is CR 4a; R * is optionally substituted by one or more halogen atoms (C1-4alkyl; or optionally substituted by one or more halogen atoms (C1-C4 alkoxy; R2 is C3-3R5) H). , G-13 craving CF *; R4 is H or Me; R4 * is H; R5 is -MRi4R4f or OH, and R6 is optionally substituted with one or more hair atoms (t ~ 4 carbon atoms 6v II: a compound or a pharmaceutically acceptable salt thereof; wherein A is N or CR 48; R48 is H or (1-4 carbon atoms; R1 is optionally substituted with one or more halogen atoms (C1-8 carbon atoms; optionally substituted with one or more halogen atoms) (C1-C8 alkyloxy; halogen); NRuR5; ((C 6-14 carbon atoms; or - ((C4-C4 alkyl) -5- to 5-membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from N, O and S, wherein each of the aryl and heterocyclic groups is optionally substituted with one or more Z substituents · R5 is H or CHR; group; Ru is H, optionally substituted with one or more unsaturated (1-8 carbon atoms) alkyl (C3-10) -dklosl? or ((C 1 -C 4) 3 -alkyl) - (3-8 carbon atoms) and R 10 are each independently H, (optionally substituted with 1 to 8 carbon atoms in the brain or with several halogen atoms) (2-8) (3-8 carbon atoms) cycloalkenyl (2-10 carbon atoms) -cycloalkenol (5-10 carbon atoms); (3 ~ 8 carbon) -cycloalkyl; - ((0-4 s2enatom $) - alfell} ~ (Cit141414 ~ ~itit; or - ((0-4 s2enatpmos) -alkyl) -3-14 members heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from h1, O, and S1, wherein each radical, dcloalkehyde, ars, and heterocyclic is optionally substituted with one or more Zxxxts, or R '' 'and Ri, : together with the nitrogen atom to which they are attached, a heterocyclic group substituted with 4-14 members optionally substituted by one or more Z; Z is independently OH, &quot;, " optionally substituted with one or more OH or HHj groups (1-6 sxenatoms, optionally one or more snapshots). substituted (1-6 Senai) ~ 1: 1 group, optionally one or more OH or (i ~ 4 carbon atoms) substituted fi- 6 atoms, coxi, H: Rff ($ Ö2) Rxlf (CO *) NR9R21f (SOa)! ** 1 HR2SC {Ö} Rn C (Q} mR9R2 m &lt; c > (O) mR9% NRlR (O) %R19, C (Q) R1, SRi> s, OR59, oxo, 04, MOZt halogen or a 3-14 membered heterocyclic group, wherein the heterocyclic group contains at least one heteroatom selected from M, O and S, each R1 and Rn independently of one another H (C 1 -C 8 -alkyl; (C 3-8 cycloalkyl; C 1-4 -alkyl) (C 1-4 -alkyl; optionally: one or more (1-6); C 1 -C 6 (C 1 -C 6) alkoxy and halogen-substituted substituents ((C 0 -C 4) alkyl) an (C 0 -C 4 alkyl) - 3 A -14-membered buterocyclic group, a heterocyclic group containing one or more heteroatoms selected from N, O and S; optionally containing one or more substituents selected from halogen, oxo, (C1-C6) alkyl and (C1-C6) alkyl; optionally, one or more (C 1-6 alkyl) -O-aryl containing (1-6 carbon atoms) (1-6 carbon atoms) and halogen atoms (and (0-4) alkyl sxene atom-alkyl} - 0-3-14-membered heterocyclic group, wherein the heterocyclic group contains one or more heteroatoms selected from N, O and S, and optionally one or more halogen atoms (C 1 -C 6) containing a substituent selected from alkyl or C (O) (1-6C) alkyl, wherein the alkyl groups are optionally one or more halogen, (1-4 carbon atoms, * alkoxy, C (O) MH2, C (O)). 0) NH (1-6 carbon atoms) or C (Q) fJ ((1-6C) -alkyl); or Rr9 and R6 and the associated mfrogen together with a 5-membered heterocyclic group where the heterocyclic group contains one or more additional heteroatoms selected from the group consisting of 14, 0 and S, wherein the heterocyclic group is optionally a more than one OH; halogen atom; one or more S-18 membered heterocyclic radicals containing M, O and S selected; S {0) RaNi; S (Ö) 2- (1-6 x S); (C1-C6) -alkyl optionally substituted by one or more halogen atoms; optionally substituted with one or more OH or (C 1-6) alkoxy (1-4 carbon atoms), and (0) O (C 1 -C 6) -a is selected from the group consisting of small groups ah, and heterocyclic; Substituent groups themselves (C 1-6 carbon atoms) (C 1-6 carbon atoms or (1-6 xxenosemol-alkoxyseportates are substituted.). The compounds of claim 1 are disclosed below. thiophenyl-5'-thiophenylmethyl-2'-methyl-2'-carboric acid (3 # 3, 3S-trifluoro-1'-trifluoromethyl-propyl) -amide; -amno- d-bromo-5-thouofmethyl-pinbln-5-carboxylic acid (3,3-trifluoro-2-h: idmxyl: pro: pil5-amide; 3. aminoamino-6-bromo-S-thiol-formylpyridine) 2 ~ Carboxylic acid C {S} -3. # 3β-chloro-2 "<RTI ID = 0.0> Cl-xl-prop-1-amide; </RTI> 3-amino-6-bromo-S-thiophene: et; 2 'Carbamic acid ((3) -1- (tetrahydro-yn-2'-yl) matyl amide; 3-amine: Na-6-bromo-S-trifluoroethylpyridine n-2-carboxylic acid ( f1 f 3 Jd lostolan-2 - limetll} ~ a mid; 3-amno-6-brdrn: ~ 5-trlFMOfin: en-pHdl'n-2-kafbonsa.v [{S} -1- (tofxa: hldro "furan-2 ~ ii} matyl | amide); 3ram: iro-6-bromo-S-tri: fluoro-methyl-trifluoromethyl-2-carboxylic acid (tetrahydro-furan-S-dimethyl) -amide; 3-Amino-S-bromo-5-trifluoromethyl-pyridine-2-xrbonic acid {2-η ^ 6ΐΗ ~ 2-ρ1ρβπ ^ 1: η: -1 ~ ί-ρ? Ορί |} '· a mid; 3-Amino-6-bromo-5H-methylmethyl-pyridyl: 2-carbidyl: acid (S: - h 1 d r x 1 - pro ρ 10 ~ α 1-d; 3-amrk-6-bromo-5-trif | yormedi "peak: dln-2-carboxylic acid (2: -hydroxy-2-methylpropyl} amide; 3 ^ amylo-6-bromo: mS ~ tnfluxazil · ρiri: dm: -2-xΓboa: acid {2 ~ MeOH-tetrahydmfuran-2-ylmethyl; H} -amide; 3 ~ aming-6: - &amp; r ~ 5 ~ tnflpormethyl-pyridine-2-carboxylic acid U-methoxyethoxy-3; brem-5-trihydro-pifin-2-kerbonic acid [2 · (4 ·? ΙυοΓ ··? βηΙΙ) ··· · · · · · · · ί tnf 1-normethylpyridine-2-carboxylic acid (2-methyl-4-N-2-phenyl-ethyl) -amide; 3-amino-6-bromo-5-fluoro-methyl-pyridine-2 carboxylic acid (5-dlmotylamino-1-phenyl-ethylamino; 3-amo-6-fluoro-4-fluorophenyl) -2-fluoromethyl-pyrrolidin-2-carboxylic acid (3,3,3-trifluoro-2) hydroxymethyl 2-motH-propyl} amide; S-amno-S-Hydrofluoric acid tri-α-carboxylic acid (3? 3? 3? M? 2? 2-hW? xi? 2-methyl-propyl) amide:; 3-amo-ino: -6- (4-chloro-2-methylphenyl) -5-trifluoro-amino] i: pi: rldin'2: -kar bonic acid ((1) - 3; 3; 3-trifluoro-2-hydroxypropyl) amine; 3-Amino-6-bromo-5-trifluoromethyl: p-2-carboxylic acid (S, N-trifluoro-X-) bldrox1-α-trifluoromethyl-1-amide; 5-amyl-O-6-6 -methyl-3: tdeol: phenylethyl2,2,3] bipindinyl 6-carbonic acid (32 µl-1xlflubr-2-bldrox-1-2'-mMl-propyl) - amide; 3-amine-6-bromine "thymormethyl" p-2'-carboxylic acid ((Ε} "33.3" ίπΙΙ «ο: Γ" 2 ·· ΗΙ0Γθχ1 · '2-ΓΤ5 «ί1Ι · · 'propH) - aroyl; 3-alpha-Rkv-6-ferrone' 5'nfluarmet3l'-pyrsdlril '2 ~ karfeofYsax #] ((S} 3,3? · Propyl} amide; 3 "ammo ~ 6 ~ methoxyl" S-ring radicals: ridsp'-2-carbarboxylic acid (3,3f3 - tnfkM: r ~ 2 -hydex ~ 2 "rn« | j | - · propyl) ami: dj 3 ·· θ ^? ηο- · 6 ^ 4-0υορ4θηΜ> 5 * ^ ΐΡο ^ 60: 1> ρΗ0 ^ 2 '~% θΓ &amp; ρηδ3ν ((3) -3 ^ 3,5> trichloride> bld: roKl-2-methylpropylamide; 3 ~ a: mmo ~ 6- (4 "fdr-land)» 5'4lllkiorm: et ^ ~ plridbv-2: - Carboxylic acid C (R): - 3,333-trifluoro-2 -: hyd: mxl - 2 - breast - pro pH) a mid; 3-amine-6 - (2,4--) diclarophenyl} -Syride uorphcypyridyl-2-carboxylic acid f3,3> .3- [alpha] -r-2-hydroxy-2-methylpropyl} 3-amide; <RTI ID = 0.0> 4-fluoro-phenyl-5-trifluoromethyl-pWdi: </RTI> <RTI ID = 0.0> p-1: kaFlx3 (&quot; acid C2 &lt; / RTI &gt; πdiπ-2 ~ ka: rbôosay (3,3; 3-triftoof "2'4droxoxy ~; pmpH) '· amide; N-S-Meth - 3 - trifluoro - [2,3 '] bis d -carboxylic acid {3, .3,: 3-tdfl: powder »2-yl: id. 1'-2-trifluoromethylpropyl; S-amno-6'-methyl-3'-phenylphenyl: myristyl-2'-methylpropyl-6'-oxide 0: r5-acid (3.3.3 - ri ri fh; or ~ 2-hydroxy-2-methylpropyl) amide; 3-amino-5,6-biS2 "fluoroethyl-1'-n: dfn'-2'-carboxylic acid propyl aroid; 3'-no-5,6-blsp-n-methylpiperidine-2-carboxylic acid CCR) -3,3,3-tn-η 2 -dichloro-2-hydroxy-2-propyl-1H-form; 3-amine-6-methoxy-3-trifluoromethyl-pyridin-2-carboxylic acid CCS) -3 3,3-thiophyl: uor-2 "hydroxy-2 '· methylpropyl; 3 ~ amno-6' m; pxi-5 'trifluoro: OrmetH' ': pyri; di: 0:' 2-carbo n: acid {(^) - 3,3,3 "ίπίοοΓ-2 ~ Ν4Γ0χΙ: ~ 2-matlpropH) -am; N ethyl 3 - (3 - quality 6 b - 5 - (trtf u u rm ed I) -pl m arne do} - propanoate; 3- a: mino-'S' - (oxazole-2 ~ H) "N- (3,3,3 ~ tpf: ia0r - 2 'Nid: roxi-2 ~ methyl: pripiii: 5-5' 'tntl: U: omiobil:) - · picoBnamid; 3 ~ ammo-6-bromo - (3f3,3-tri) u-2-methoxy-2-methylpropyl) -5- (trifluoroethyl) -p; cylhydryl; 3-3mloxy-4- (2-hydroxy-3''-methan-2-one). ) but? i) -S-methoxy - 5 - (tnfiuorm'Sti: l} '· pichnamid ;: 3-'a.mm0-6 ~ dkipp ropH "M -" (3,3,3-tert-2-hydroxy "2-methylpropyl} -5- (iTifluoromethyl) pico-amide); 3-Azobenzo-6-methoxy-N- (3f-3-triflypr'-2 'hydrido-2 - (tdfiuorrnotyl) propyl} -5' - (thiophenylamino) '' picinamide; "(3,33-Fluoro-2-hydroxy-2-methyl-propyl) -3 '- (trichlorophenyl) -2f4' bipyricin-6-carboxemide; 3 ~ am: i: ii '6 ~: bmm - 5-tn: fluo: rm: 0tii-psrldl-2-carbor> acid 3-amino-6- (1-methyl-1H-pyrazol-1M) ~ N- (3,3,3 ~ tdfi'or- 2 ·· bridge roxi - 2 - methylpr opll) - 5 - {triethylpolycholide; (S} '3-a: mi' '&amp; ~ etoxyl ~ N ~ <3, .3 < , 3 ": tr <f1« öri-2-h: í'dmxi "2« rn € ti:! Prispli! "" S ~ (triítóófrn € k) "plkôlinamid; 3-a: míno ~ é - :( p3rrolÍdm 1- (N- (3,3-trifluoromethyl) -2-pyrrolidin-2-ylpropyl) -5- (trifluoromethyl) pfkoinamide; - (3,3-tri-trifluoro-2- (4-methoxy-2H-yl-5-matH: propyl) '- S ·' (trichlorophyll 1) - or a pharmaceutically acceptable salt thereof, Sv A 7, A compound according to claim 1, which is 3-amooyl-6-methoxy-trifluoromethylpyridyl 2-ylcarboxylic acid (3), 3, 3, 3, 3, 3, 3; ί ~ · “2 ~ ^ '66 ΐ · Ι-ρΓορΙΙ)“ 8 ^: Ι. <ί, or a pharmaceutically acceptable salt thereof. Compound according to claim 3 which is 3-amino-6-methoxy-S-trifluoro-orphenyl: pyridine-2-carboxylic acid {(R} '~ 3,3'f3 ~ Hiuor ~ 2 "hldroxl" 2''ietiet "" propyl3-amine or a pharmaceutically acceptable salt thereof. 10. A compound according to claim 7, deep 3-amino-6 '(4-fluoro-phenyl) -S' trichloromethyl 'pyridine-2-carboxylic acid (3 < 3,3-trifluoro [2- (2-hydroxy) -2-methylpiperazinyl] smil4 or a pharmaceutically acceptable salt thereof, 11 'A 7, A compound according to claim 1 which is B-amino-SS-bis-tritylmethylmethyl pifldln-α-carboxylic acid ((S} · -3β3-trifluoro-2'-hydroxyl-2 "methyl · pyrido [5 '); or a pharmaceutically acceptable salt thereof. Compound according to claim 1; which is O-arnino-Sfh-PIs-tnflpormethylpiperine - carboxylic acid ((R5-3,3,3-trifluoro-2'-hydroxy-2-methylpropyl) amide or a pharmaceutically acceptable salt thereof) . 13, A compound according to any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, for use in the manufacture of a pharmaceutical composition 14, A method according to any one of claims 1 to 12, or a pharmaceutical lag: an acceptable salt, for use in the treatment of mucous membranes, inflammatory or occlusive digestion in the treatment of respiratory diseases or conditions; 15, A compound of any one of Claims 1 to 12v, or a refractory salt of a herbicide for use in the treatment of cystic fibrosis; A compound according to any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, for use in the manufacture of a medicament for the treatment of inflammatory or obstructive respiratory diseases or conditions of mucosal membranes; A pharmaceutical composition comprising a compound according to any one of claims 1 to 12 and one or more pharmaceutically acceptable additives; 18, a pharmaceutical combination which: as a first active ingredient, a composition according to any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, and osmotic agents, ENaC blockers, anti-inflammatory drugs, snoring agents, antihistamines, painkillers, anti-blotting agents, and It comprises a second active ingredient selected from DNase drugs, wherein the first and second active ingredients are present in the same or separate pharmaceutical compositions.