HU196555B - Composition for desinfection of udder - Google Patents

Abstract

A widely effective antifungicide and antimicrobiological product comprises 0.5% Bradophen, 0.05% Bronopol and 5% hydrophilated silicon. The effect of the product is synergic and the hydrophilated silicon extends the length of the product's effectiveness. - Application: under disinfection, skin nourishment, veterinary, food industry.

Description

BACKGROUND OF THE INVENTION The present invention relates to an udder disinfectant composition comprising antifunginic and antimicrobial agents. The known used udder disinfectant preparations have not been complied with in the long term for the following reasons:

- applied to the udder skin (dehydration, dermatitis), the milking hands (allergic phenomena, dehydration), applied in revenge,

- the pores and fine wrinkles do not provide a lasting, germ-free environment.

- interferes with dairy processing as a foreign substance.

The antimicrobial composition described in Hungarian Patent Application No. 185 093 contains 0.05-0.5% by weight of the active ingredient benzyl dodecyl bis (2-hydroxyethyl) ammonium clone (Bradophen, Ciba Geigy) and cetylpyridinium bromide. : 15: 1 by weight, in combination with a liquid carrier and a component or agent for the absorption of the active ingredient. with other excipients. This product is proven to be a useful surgical wash, hand disinfectant, and is excellent in destroying residual microflora, although it is not suitable alone for this purpose.

It is believed that the known formulation may be suitable for udder disinfection because

- has broad antimicrobial activity,

- long-term germination of the skin surface,

- its regular use does not damage the skin,

- easy to apply, not hazardous to humans, animals,

- non-corrosive (does not attack metal, wood, concrete, rubber and plastic surfaces),

Based on the assumptions, different concentrations of benzyl dodecyl bis-2-hydroxymethylammonium chloride disinfectant solutions have been prepared, which contain well-established skin nourishing and skin protection agents in addition to the active ingredient content. The efficacy experiments, although positive, resulted in a large number of animals showing that the product did not fully meet the requirements because

- not broad enough in terms of bacteria or fungi,

- does not ensure a poor germ between two heads.

SUMMARY OF THE INVENTION The object of the present invention is to provide an udder disinfectant composition which is more effective than known antimicrobial formulations for external disinfection of milking nipples in dairy cows, which is easy to use, prolonged use, non-corrosive to materials used in the dairy industry.

The udder disinfectant composition of the present invention comprising benzyl dodecyl bis (2-hydroxyethyl) ammonium chloride is characterized in that benzyl dodecyl bis (2-hydroxyethyl) animonium is present in an amount of 0.20.9% by weight as an active ingredient. and a mixture of 2-bromo-2-nitropropane-1,3-diol in a 10: 1 to 5: 1 weight ratio in water-alcohol solution with 2 to 10% by weight of polystyrene-polyether silicone oil and, if desired, other skin nutrients. Other excipients include sorbitol and a composition containing ₄ allantoin, a fatty alcohol ether fatty acid ester and urea (Dragoco Guberdin, a Hydanton product from the Federal Republic of Germany) and a dye.

The aqueous alcoholic solution is a mixture of isopropyl alcohol and ethyl alcohol in deionized water. The excipients are intended to reduce the damage caused by the use of the udder and, in part, to act as a lubricant.

2-Bromo-2-nitropropane-1,3-dio (Bromopol) is a commercial formulation used as a fungicide.

The hydrophilic silicone oil component of the composition is a polysiloxane polyether marketed by Th. Goldschmidt AG Chem.

The composition according to the invention is particularly successful for the disinfection of the udder because the combination of the two known active ingredients and silicone oil effectively and permanently kills the bacteria causing the mastitis.

For long-lasting germination, use of hydrophilized silicone, which forms a durable protective film on the udder surface, does not impede respiration of the skin.

In the pharmacological studies, we found that the two fungicides known in the art, respectively. The antimicrobial component has a much broader spectrum of activity than described in the literature, with excellent results in udder disinfection. It also provides effects that are extremely beneficial in maintaining the water balance and elasticity of the udder.

In summary, the composition of the present invention is effective in killing mastitis bacteria (Staphylococcus aureus, Streptococcus bovis, Streptococcus agalactiae, Streptocofccus dysgaiactae, Pseudomonas aeruginosa, Escheriphia coli). The excipients in the udder disinfectant solution form an elastic, thin film-like layer on the nipple that prevents pathogens from entering the nipple and nipple epithelium, and provides protection to regenerate the nipple epithelium and soothe the next pre-milking. In addition, the ingredients in the formulation to protect the skin make the teat skin soft and supple. The neutral dye in the solution helps to carry out and control the disinfection.

The composition of the composition is also exemplified in the following examples:

Example 1

0.2 g Bradophen 0.2%

0.02 g Bronopol 0.02%

2.0 g Polysiloxane Polyether 2.0% (AB1L B 8847)

1.0 g Hydroviton 4.0 g sorbitol 39.0 mg ethyl alcohol 23.0 ml isopropyl alcohol 0.1 g yellow dye (C.I.

19140, C.I.. 13015) ', 100 ml deionized water

The composition can be prepared by mixing and homogenizing the components.

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Example 2

0.05 g Bradophen 0.5%

0.05 g Bronopol 0.05%

5.0 g polysiloxane polyethane - 5.0% (ABIL B 8847)

1.0 g Hydroviton 4.0 g sorbitol 39.0 mg ethyl alcohol 23.0 ml isopropyl alcohol 0.1 g yellow dye (C.I.

19140 C.I. 13015) gives 100 ml deionized water

The composition can be prepared by mixing and homogenizing the components.

Example 3

0.8 g Bradophen 0.8%

0.1 g Bronopol 0.1%

10.0 g polysiloxane p / ether 10.0% (ABIL B 8847)

1.0 g Hydroviton 4.0 g sorbitol 39.0 ml ethyl alcohol 23.0 ml isopropyl alcohol 0.1 g yellow dye (C.I.

19140, C.I.. 13015) gives 100 ml deionized water

The composition can be prepared by mixing and homogenizing the components.

The results of the activity tests are described in Example 4:

Example 4

Comparative study of the disinfectant effect on caviar fungi on cowshed

Test method:

The experiments were carried out on 104 cows for 3 months after finishing the morning and afternoon milking by immersing the nipples in a disinfectant solution.

To check the disinfection effect, bacteriological samples were taken 20 minutes, 3 hours and 8 hours after disinfecting the teat. At the above intervals, samples were taken from 4 to 4 sites per teat with 7 cm ² selective media discs each. As a control, samples were taken from the bovine udder boob 10 only after normal washing and wiping, without disinfection, after skin drying.

For the bacteriological evaluation of sporadic fungi, ten Cate agar-imprint method was used. In this method, the following categories of purity evaluation sub ¹⁰ kalmaztuk found in 7 ^cm2 surface táptalajkonrongokon average saijadó fungal colony counts: Purity categories increased by 7 cm ² rarely contaminated surface colony number between 3-9 0-2 sterile contaminated to a degree between a highly contaminated 10_'29 Between 30 and 80, heavily soiled carpets are heavily soiled, and more than 80 are heavily soiled

During the continuous disinfection for 3 months, the test was performed 4 times using the above method. Occasionally, 30-30 cows' disinfected udder samples (solutions A and B) were tested.

The result of our study is 120-120 samples.

Explanation: Solution A: Example 2

Solution B: Comparative known preparation that does not contain Bronopol

The results of the tests are detailed in Tables 1 and 2:

Table 1

Purity categories of sponge fungi when using the disinfectant according to the invention (Solution A)

Time of sampling and number of samples

The number of purity categories (% of samples) is sterile but highly contaminated

120 minutes after disinfection 81 (67.5) 39 (32.5) 120 hours after disinfection 40 (33.3) 64 (53.3) 16th (3.13) 120 hours after disinfection 8 hours 57 (47.5) 46 (38.3)

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Table 2

The purity categories of creeping fungi are known »when using disinfectant (solution B)

Sampling Number of purity categories (% of samples) time and number of samples sterile but very slightly highly contaminated

20 minutes after disinfection

120 db 32 (26.6)

3 hours after disinfection

120 db 17 (14)

120 hours after disinfection 8 hours

84 (70) 4 (3.3) 65 38 (54.2) (31.6) 18 63 (15) (52.5)

(32.5)

From the tables it can be seen that after sterilization of nipple buds of essentially the same degree of contamination with solutions A and B - 20 minutes after disinfection, the number of sterile samples in solution A is 2.5 times the value found in solution B, 2 times less than solution B, - 3 hours after disinfection, the number of sterile samples in solution A is 2.3 times the value found in solution B, while the number of low-impurity samples after solution B is almost 2 times , 4 times the value found in Solution A, - 8 hours after disinfection, the number of heavily contaminated samples was approximately 2 times higher with Solution B compared to Solution A.

In general, solution A has a fungicidal or fungistatic action 2 to 2.5 times that of solution B.

Claims (1)

A patent claim Udine antiseptic composition containing benzyldode35 cilibis (2-hydroxyethyl) ammonium chloride, characterized in that the active ingredient is benzyl dodecylbis (2-hydroxyethyl) in an amount of 0.2-9% by weight. a mixture of ammonium chloride and 2-bromo-2-nitropropane-1,3-diol in a ratio of 10: 1-5: 1 by weight and 2 to 10% by weight. The polysiloxane polyether contains about 5% by weight of a silicone oil, preferably in an aqueous alcoholic solution, with the addition of skin nourishing excipients.