HRP20060076A2 - Mješovita bakterijska kultura za razgradnju atrazina - Google Patents
Mješovita bakterijska kultura za razgradnju atrazinaInfo
- Publication number
- HRP20060076A2 HRP20060076A2 HR20060076A HRP20060076A HRP20060076A2 HR P20060076 A2 HRP20060076 A2 HR P20060076A2 HR 20060076 A HR20060076 A HR 20060076A HR P20060076 A HRP20060076 A HR P20060076A HR P20060076 A2 HRP20060076 A2 HR P20060076A2
- Authority
- HR
- Croatia
- Prior art keywords
- atrazine
- culture
- enzymes
- coding
- bacterial culture
- Prior art date
Links
- MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 title abstract 6
- 230000001580 bacterial effect Effects 0.000 title abstract 2
- 230000015556 catabolic process Effects 0.000 title abstract 2
- 238000006731 degradation reaction Methods 0.000 title abstract 2
- JIHQDMXYYFUGFV-UHFFFAOYSA-N 1,3,5-triazine Chemical group C1=NC=NC=N1 JIHQDMXYYFUGFV-UHFFFAOYSA-N 0.000 abstract 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 101100218331 Pseudomonas sp. (strain ADP) atzB gene Proteins 0.000 abstract 1
- 101100218332 Pseudomonas sp. (strain ADP) atzC gene Proteins 0.000 abstract 1
- 230000033558 biomineral tissue development Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000001925 catabolic effect Effects 0.000 abstract 1
- 230000000593 degrading effect Effects 0.000 abstract 1
- ZFSLODLOARCGLH-UHFFFAOYSA-N isocyanuric acid Chemical compound OC1=NC(O)=NC(O)=N1 ZFSLODLOARCGLH-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000013048 microbiological method Methods 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000005067 remediation Methods 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 101150090745 trzD gene Proteins 0.000 abstract 1
- 239000002351 wastewater Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S210/00—Liquid purification or separation
- Y10S210/902—Materials removed
- Y10S210/903—Nitrogenous
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Predmet ovog izuma je mješovita bakterijska kultura, nazvana Atz Mix 1, porijeklom iz tla koje je bilo izloženo dugotrajnom zagađenju atrazinom i drugim s-triazinskim spojevima. Kultura Atz Mix 1 razgrađuje atrazin u vrlo širokom koncentracijskom rasponu (nekoliko ppb do deset tisuća ppm), pri čemune nastaju toksični metaboliti, nego se atrazin potpuno razgrađuje (mineralizira). Atz Mix 1 je stabilna mješovita kultura koja se sastoji od najmanje četiri bakterijska soja različitih morfoloških, fizioloških i kataboličkih svojstava. Molekularna karakterizacija kulture Atz Mix 1 ukazala je na prisutnost kataboličkih gena koji kodiraju enzime zarazgradnju atrazina do cijanurne kiseline i otvaranje s-triazinskog prstena (trzN, atzB, atzC i trzD), a visoki stupanj mineralizacije radioaktivno obilježenog 14C atrazina u prstenu (približno 80%) potvrdio je da se razgradnja nastavlja do CO2 i NH4+.Mikrobiološka metoda za razgradnju atrazina i drugih s-triazina koju uključuje ovaj izum ima prednost pred do sada opisanim metodama u kojima se primjenjuju pojedinačne bakterijske kulture napose zbog održivog rasta i stabilne kataboličke aktivnosti mješovite kulture Atz Mix 1 u različitim, promjenljivim i nepovoljnim uvjetima (visoka koncentracija atrazina, nepovoljan udio C/N, niske temperature). Nadalje treba naglasiti da je negativan učinak na kinetiku razgradnje atrazina i kataboličku aktivnost kulture Atz Mix 1 u prisutnosti pogodnijegizvora dušika značajno manje izražen nego kod do sada opisanih pojedinačnih bakterija. Temeljem navedenog očekuje se da će mikrobiološka metoda koja je predmet ovog izuma biti pogodna za bioremedijaciju zagađenog tla, napose tla koji sadrži veće količine dušikovih spojeva te za ubrzanje procesa mineralizacije atrazina u vodenom mediju, napose u otpadnoj vodi koja sadrži visoke koncentracije toga herbicida.
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HR20060076A HRP20060076A2 (hr) | 2006-02-20 | 2006-02-20 | Mješovita bakterijska kultura za razgradnju atrazina |
| PCT/HR2007/000002 WO2007096681A1 (en) | 2006-02-20 | 2007-01-22 | Mixed bacterial culture for atrazine degradation |
| AU2007217158A AU2007217158B2 (en) | 2006-02-20 | 2007-01-22 | Mixed bacterial culture for atrazine degradation |
| DE602007013363T DE602007013363D1 (de) | 2006-02-20 | 2007-01-22 | Gemischte bakterienkultur für atrazinabbau |
| EP07700434A EP1991658B1 (en) | 2006-02-20 | 2007-01-22 | Mixed bacterial culture for atrazine degradation |
| CNA2007800080485A CN101395262A (zh) | 2006-02-20 | 2007-01-22 | 用于阿特拉津降解的混合细菌培养物 |
| AT07700434T ATE503002T1 (de) | 2006-02-20 | 2007-01-22 | Gemischte bakterienkultur für atrazinabbau |
| CA2641482A CA2641482C (en) | 2006-02-20 | 2007-01-22 | Mixed bacterial culture for atrazine degradation |
| US12/194,995 US7658850B2 (en) | 2006-02-20 | 2008-08-20 | Mixed bacterial culture for atrazine degradation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HR20060076A HRP20060076A2 (hr) | 2006-02-20 | 2006-02-20 | Mješovita bakterijska kultura za razgradnju atrazina |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20060076A2 true HRP20060076A2 (hr) | 2007-09-30 |
Family
ID=38086359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HR20060076A HRP20060076A2 (hr) | 2006-02-20 | 2006-02-20 | Mješovita bakterijska kultura za razgradnju atrazina |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7658850B2 (hr) |
| EP (1) | EP1991658B1 (hr) |
| CN (1) | CN101395262A (hr) |
| AT (1) | ATE503002T1 (hr) |
| AU (1) | AU2007217158B2 (hr) |
| CA (1) | CA2641482C (hr) |
| DE (1) | DE602007013363D1 (hr) |
| HR (1) | HRP20060076A2 (hr) |
| WO (1) | WO2007096681A1 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6260987B2 (ja) * | 2013-10-29 | 2018-01-17 | 国立研究開発法人農業・食品産業技術総合研究機構 | アミノ−ｓ−トリアジン系化合物とシアヌル酸を分解する細菌、微生物製剤および分解菌保持担体並びに汚染環境の浄化装置およびアミノ−ｓ−トリアジン系化合物の分解方法 |
| CN104368121B (zh) * | 2014-10-31 | 2017-05-17 | 陕西师范大学 | 氯过氧化物酶催化转化莠去津的方法 |
| US20200370033A1 (en) * | 2018-01-03 | 2020-11-26 | Regents Of The University Of Minnesota | Biological assembly including biological component and shield |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5508193A (en) | 1993-08-31 | 1996-04-16 | Regents Of The University Of Minnesota | Pseudomonas strain for degradation of s-triazines in soil and water |
| US20060257890A1 (en) * | 1996-05-20 | 2006-11-16 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5429949A (en) | 1994-02-22 | 1995-07-04 | Ohio State Research Foundation | S-triazine degrading bacteria |
| AU2001231155A1 (en) * | 2000-01-25 | 2001-08-07 | Hugh Mctavish | Microbes and methods for remediation |
| US6623211B2 (en) * | 2000-05-24 | 2003-09-23 | Rutgers University | Remediation of contaminates including low bioavailability hydrocarbons |
| US20070101461A1 (en) * | 2001-05-16 | 2007-05-03 | Daniel Van Der Lelie | Method for Improving Phytoremediation Treatment of a Contaminated Medium |
-
2006
- 2006-02-20 HR HR20060076A patent/HRP20060076A2/hr not_active Application Discontinuation
-
2007
- 2007-01-22 AT AT07700434T patent/ATE503002T1/de not_active IP Right Cessation
- 2007-01-22 AU AU2007217158A patent/AU2007217158B2/en not_active Ceased
- 2007-01-22 WO PCT/HR2007/000002 patent/WO2007096681A1/en active Application Filing
- 2007-01-22 CA CA2641482A patent/CA2641482C/en not_active Expired - Fee Related
- 2007-01-22 EP EP07700434A patent/EP1991658B1/en active Active
- 2007-01-22 DE DE602007013363T patent/DE602007013363D1/de active Active
- 2007-01-22 CN CNA2007800080485A patent/CN101395262A/zh active Pending
-
2008
- 2008-08-20 US US12/194,995 patent/US7658850B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN101395262A (zh) | 2009-03-25 |
| AU2007217158A1 (en) | 2007-08-30 |
| WO2007096681A1 (en) | 2007-08-30 |
| CA2641482C (en) | 2012-06-05 |
| DE602007013363D1 (de) | 2011-05-05 |
| CA2641482A1 (en) | 2007-08-30 |
| EP1991658A1 (en) | 2008-11-19 |
| ATE503002T1 (de) | 2011-04-15 |
| US7658850B2 (en) | 2010-02-09 |
| AU2007217158B2 (en) | 2012-11-15 |
| US20090026135A1 (en) | 2009-01-29 |
| EP1991658B1 (en) | 2011-03-23 |
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| A1OB | Publication of a patent application | ||
| AIPI | Request for the grant of a patent on the basis of a substantive examination of a patent application | ||
| ODRP | Renewal fee for the maintenance of a patent |
Payment date: 20130213 Year of fee payment: 9 |
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| ODBI | Application refused |