HRP20040109A2 - Screening method for inhibitors of folate synthesis pathway enzimes - Google Patents

Screening method for inhibitors of folate synthesis pathway enzimes Download PDF

Info

Publication number
HRP20040109A2
HRP20040109A2 HR20040109A HRP20040109A HRP20040109A2 HR P20040109 A2 HRP20040109 A2 HR P20040109A2 HR 20040109 A HR20040109 A HR 20040109A HR P20040109 A HRP20040109 A HR P20040109A HR P20040109 A2 HRP20040109 A2 HR P20040109A2
Authority
HR
Croatia
Prior art keywords
dhps
activity
determining
inhibitory activity
accordance
Prior art date
Application number
HR20040109A
Other languages
English (en)
Croatian (hr)
Inventor
Thur Se Stachyra
Jacques Biton
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Publication of HRP20040109A2 publication Critical patent/HRP20040109A2/hr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/18—Testing for antimicrobial activity of a material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
HR20040109A 2001-08-01 2004-01-30 Screening method for inhibitors of folate synthesis pathway enzimes HRP20040109A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01402085A EP1281769A1 (de) 2001-08-01 2001-08-01 Screening-Verfahren für Inhibitoren von Enzymen der Folat Synthese
PCT/EP2002/009421 WO2003012132A2 (en) 2001-08-01 2002-07-30 Screening method for inhibitors of folate synthesis pathway enzymes

Publications (1)

Publication Number Publication Date
HRP20040109A2 true HRP20040109A2 (en) 2004-06-30

Family

ID=8182836

Family Applications (1)

Application Number Title Priority Date Filing Date
HR20040109A HRP20040109A2 (en) 2001-08-01 2004-01-30 Screening method for inhibitors of folate synthesis pathway enzimes

Country Status (6)

Country Link
US (1) US20060088900A1 (de)
EP (2) EP1281769A1 (de)
JP (1) JP2005508620A (de)
AU (1) AU2002327846A1 (de)
HR (1) HRP20040109A2 (de)
WO (1) WO2003012132A2 (de)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH670709A5 (de) * 1986-04-24 1989-06-30 Univ Moskovsk
EP0999284A1 (de) * 1998-09-11 2000-05-10 Hoechst Marion Roussel Verfahren zum Screening auf Antimykotika unter Verwendung essentieller Gene aus C.albicans, sowie die Gene selbst
CA2355816C (en) * 1998-12-14 2007-10-30 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
AU1994800A (en) * 1999-01-15 2000-08-01 Pantherix Ltd. Phosphate release enzyme assay
US7202023B2 (en) * 2000-08-11 2007-04-10 Millennium Pharmaceuticals, Inc. High throughput screen for inhibitors of the folate biosynthetic pathway in bacteria

Also Published As

Publication number Publication date
JP2005508620A (ja) 2005-04-07
EP1281769A1 (de) 2003-02-05
AU2002327846A1 (en) 2003-02-17
EP1414993A2 (de) 2004-05-06
WO2003012132A3 (en) 2003-08-28
US20060088900A1 (en) 2006-04-27
WO2003012132A2 (en) 2003-02-13

Similar Documents

Publication Publication Date Title
Patricelli et al. In situ kinase profiling reveals functionally relevant properties of native kinases
Smith et al. Identification of a redox-sensitive switch within the JAK2 catalytic domain
KR20010032048A (ko) 게놈 접근법을 사용하여 동정된 단백질을 기능적으로분류하는 고처리량 방법
US20120164670A1 (en) Methods and kits for measuring enzyme activity
Park et al. Protein ubiquitination and formation of polyubiquitin chains without ATP, E1 and E2 enzymes
Phelan et al. Adenylation enzyme characterization using γ-18O4-ATP pyrophosphate exchange
Eriksson et al. Method for real-time detection of inorganic pyrophosphatase activity
Fisher et al. A sensitive, high-volume, colorimetric assay for protein phosphatases
US20080050762A1 (en) Methods and compositions for coupled luminescent assays
Camara et al. Inhibition of p-aminobenzoate and folate syntheses in plants and apicomplexan parasites by natural product rubreserine
Branstrom et al. Assay for identification of inhibitors for bacterial MraY translocase or MurG transferase
Kröplin et al. Inhibition of thiopurine S-methyltransferase activity by impurities in commercially available substrates: a factor for differing results of TPMT measurements
Loring et al. Yeast Chfr homologs retard cell cycle at G1 and G2/M via Ubc4 and Ubc13/Mms2-dependent ubiquitination
Chakraborty et al. Profiling of the drug resistance of thousands of Src tyrosine kinase mutants uncovers a regulatory network that couples autoinhibition to catalytic domain dynamics
US20130122514A1 (en) Methods for determination of protein phosphatase activity, and uses in predicting therapeutic outcomes
Saini et al. High-throughput microplate phosphorylation assays based on DevR-DevS/Rv2027c 2-component signal transduction pathway to screen for novel antitubercular compounds
HRP20040109A2 (en) Screening method for inhibitors of folate synthesis pathway enzimes
Valderas et al. Examination of intrinsic sulfonamide resistance in Bacillus anthracis: a novel assay for dihydropteroate synthase
Šebela The use of matrix‐assisted laser desorption/ionization mass spectrometry in enzyme activity assays and its position in the context of other available methods
LECLERC et al. Serine/threonine phosphorylation of calmodulin modulates its interaction with the binding domains of target enzymes
US20050004068A1 (en) Modulation of tor
Brault et al. Characterization of cytidylyltransferase enzyme activity through high performance liquid chromatography
Bruni et al. An endpoint enzymatic assay for fructose 2, 6-bisphosphate performed in 96-well plates
DE60103061T2 (de) Verfahren zur identifizierung von verbindungen, die den anaphase-fördernden komplex hemmen
Fernley et al. A rapid assay for dihydropteroate synthase activity suitable for identification of inhibitors

Legal Events

Date Code Title Description
A1OB Publication of a patent application
ARAI Request for the grant of a patent on the basis of the submitted results of a substantive examination of a patent application
OBST Application withdrawn