HK1168627A1 - Dna (deoxyribonucleic acid) index library building method based on pcr (polymerase chain reaction) - Google Patents

Dna (deoxyribonucleic acid) index library building method based on pcr (polymerase chain reaction)

Info

Publication number
HK1168627A1
HK1168627A1 HK12109360.9A HK12109360A HK1168627A1 HK 1168627 A1 HK1168627 A1 HK 1168627A1 HK 12109360 A HK12109360 A HK 12109360A HK 1168627 A1 HK1168627 A1 HK 1168627A1
Authority
HK
Hong Kong
Prior art keywords
pcr
dna
method based
chain reaction
polymerase chain
Prior art date
Application number
HK12109360.9A
Original Assignee
Bgi Tech Solutions Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bgi Tech Solutions Co Ltd filed Critical Bgi Tech Solutions Co Ltd
Publication of HK1168627A1 publication Critical patent/HK1168627A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK12109360.9A 2010-09-21 2012-09-24 Dna (deoxyribonucleic acid) index library building method based on pcr (polymerase chain reaction) HK1168627A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201010299305 CN102409049B (en) 2010-09-21 2010-09-21 DNA(deoxyribonucleic acid) index library building method based on PCR (polymerase chain reaction)

Publications (1)

Publication Number Publication Date
HK1168627A1 true HK1168627A1 (en) 2013-01-04

Family

ID=45873444

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12109360.9A HK1168627A1 (en) 2010-09-21 2012-09-24 Dna (deoxyribonucleic acid) index library building method based on pcr (polymerase chain reaction)

Country Status (3)

Country Link
CN (1) CN102409049B (en)
HK (1) HK1168627A1 (en)
WO (1) WO2012037880A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103571822B (en) * 2012-07-20 2016-03-30 中国科学院植物研究所 A kind of multipurpose DNA fragmentation enriching method analyzed for new-generation sequencing
CN102952877B (en) * 2012-08-06 2014-09-24 深圳华大基因研究院 Method and system for detecting alpha-globin gene copy number
CN103290104B (en) * 2013-01-23 2016-03-02 北京诺禾致源生物信息科技有限公司 A kind of genomic samples breaking method being applied to the simple and direct cheapness of s-generation order-checking
AU2014406026B2 (en) * 2014-09-12 2018-08-23 Mgi Tech Co., Ltd. Isolated oligonucleotide and use thereof in nucleic acid sequencing
CN104562214A (en) * 2014-12-26 2015-04-29 上海派森诺生物科技有限公司 Reduced-representation genome library building method based on type IIB restriction enzyme digestion
CN106192018B (en) * 2015-05-07 2020-03-24 深圳华大智造科技有限公司 Method and kit for enriching DNA target region by anchoring nested multiplex PCR
CN105132407B (en) * 2015-08-10 2017-12-12 北京吉因加科技有限公司 A kind of cast-off cells DNA low frequencies mutation enrichment sequence measurement
CN106811460B (en) * 2015-11-30 2020-11-27 浙江安诺优达生物科技有限公司 Construction method and kit of next-generation sequencing library for low-frequency mutation detection
CN106283198B (en) * 2015-12-31 2021-08-13 浙江安诺优达生物科技有限公司 Library construction method for single cell whole genome bisulfite sequencing
CN106048009B (en) * 2016-06-03 2020-02-18 人和未来生物科技（长沙）有限公司 Label joint for ultralow frequency gene mutation detection and application thereof
CN106192022B (en) * 2016-08-08 2018-07-03 中国科学院北京基因组研究所 The construction method of the multiple sequencing libraries of 16SrRNA
CN108949905B (en) * 2017-05-23 2022-05-17 深圳华大基因股份有限公司 Control library and construction method thereof
CN108070643A (en) * 2017-10-31 2018-05-25 南京格致基因生物科技有限公司 The construction method of microorganism 16S rDNA single molecules level sequencing libraries
CN107937986A (en) * 2017-11-10 2018-04-20 深圳裕策生物科技有限公司 A kind of FFPE DNA build storehouse kit, its purposes and banking process
CN113444769B (en) * 2020-03-28 2023-06-23 深圳人体密码基因科技有限公司 Construction method and application of DNA tag sequence
CN113584600A (en) * 2021-08-11 2021-11-02 翌圣生物科技(上海)股份有限公司 Whole genome methylation single-stranded DNA library building method

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0400584D0 (en) * 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
EP2121983A2 (en) * 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP2118121A1 (en) * 2007-03-01 2009-11-18 Novo Nordisk A/S Expression of proteins in e. coli
CN100564618C (en) * 2007-06-13 2009-12-02 北京万达因生物医学技术有限责任公司 Molecule substitution label sequencing parallel detection method-oligomictic nucleic acid code label molecule library micro-sphere array analysis
CN101967476B (en) * 2010-09-21 2012-11-14 深圳华大基因科技有限公司 Joint connection-based deoxyribonucleic acid (DNA) polymerase chain reaction (PCR)-free tag library construction method

Also Published As

Publication number Publication date
WO2012037880A1 (en) 2012-03-29
CN102409049B (en) 2013-10-23
CN102409049A (en) 2012-04-11

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