GR20120100415A - Method for the prompt detection of the mycobacterium tuberculosis - Google Patents

Method for the prompt detection of the mycobacterium tuberculosis

Info

Publication number
GR20120100415A
GR20120100415A GR20120100415A GR20120100415A GR20120100415A GR 20120100415 A GR20120100415 A GR 20120100415A GR 20120100415 A GR20120100415 A GR 20120100415A GR 20120100415 A GR20120100415 A GR 20120100415A GR 20120100415 A GR20120100415 A GR 20120100415A
Authority
GR
Greece
Prior art keywords
mycobacterium tuberculosis
detection
prompt detection
prompt
inciting
Prior art date
Application number
GR20120100415A
Other languages
Greek (el)
Other versions
GR1008136B (en
Inventor
Νικολαος Χρηστου Πουλακης
Αγγελος Δημητριου Γκριτζαπης
Βασιλειος Ξενοφων Τσιλιβακος
Original Assignee
Νικολαος Χρηστου Πουλακης
Αγγελος Δημητριου Γκριτζαπης
Βασιλειος Ξενοφων Τσιλιβακος
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Νικολαος Χρηστου Πουλακης, Αγγελος Δημητριου Γκριτζαπης, Βασιλειος Ξενοφων Τσιλιβακος filed Critical Νικολαος Χρηστου Πουλακης
Priority to GR20120100415A priority Critical patent/GR20120100415A/en
Priority to BR112015002682A priority patent/BR112015002682A2/en
Priority to CN201380049951.1A priority patent/CN104755626A/en
Priority to MX2015001717A priority patent/MX2015001717A/en
Priority to EP13765397.8A priority patent/EP2882861A2/en
Priority to AU2013301368A priority patent/AU2013301368A1/en
Priority to PCT/GR2013/000043 priority patent/WO2014023984A2/en
Priority to IN1760DEN2015 priority patent/IN2015DN01760A/en
Priority to US14/420,355 priority patent/US20150204885A1/en
Publication of GR1008136B publication Critical patent/GR1008136B/en
Publication of GR20120100415A publication Critical patent/GR20120100415A/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56933—Mycoplasma

Abstract

The invention relates to the high-accuracy intercellular detection of the mycobacterium tuberculosis inciting disease or latent infection. The detection is achieved by immunofluorescence and use of flow cytometry in all biological liquids and cells suspensions. There is also described a kit for the application of the invention.
GR20120100415A 2012-08-08 2012-08-08 Method for the prompt detection of the mycobacterium tuberculosis GR20120100415A (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
GR20120100415A GR20120100415A (en) 2012-08-08 2012-08-08 Method for the prompt detection of the mycobacterium tuberculosis
BR112015002682A BR112015002682A2 (en) 2012-08-08 2013-08-08 method for intracellular detection of mycobacterium tuberculosis using specific antibodies against rd1 products from the mycobacterium tuberculosis genome, automated flow cytometer kit and equipment
CN201380049951.1A CN104755626A (en) 2012-08-08 2013-08-08 Method for the direct detection of mycobacterium tuberculosis
MX2015001717A MX2015001717A (en) 2012-08-08 2013-08-08 Method for the direct detection of mycobacterium tuberculosis.
EP13765397.8A EP2882861A2 (en) 2012-08-08 2013-08-08 Method for the direct detection of mycobacterium tuberculosis
AU2013301368A AU2013301368A1 (en) 2012-08-08 2013-08-08 Method for the direct detection of Mycobacterium tuberculosis
PCT/GR2013/000043 WO2014023984A2 (en) 2012-08-08 2013-08-08 Method for the direct detection of mycobacterium tuberculosis
IN1760DEN2015 IN2015DN01760A (en) 2012-08-08 2013-08-08
US14/420,355 US20150204885A1 (en) 2012-08-08 2013-08-08 Method for the direct detection of mycobacterium tuberculosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20120100415A GR20120100415A (en) 2012-08-08 2012-08-08 Method for the prompt detection of the mycobacterium tuberculosis

Publications (2)

Publication Number Publication Date
GR1008136B GR1008136B (en) 2014-03-10
GR20120100415A true GR20120100415A (en) 2014-03-17

Family

ID=49223784

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20120100415A GR20120100415A (en) 2012-08-08 2012-08-08 Method for the prompt detection of the mycobacterium tuberculosis

Country Status (9)

Country Link
US (1) US20150204885A1 (en)
EP (1) EP2882861A2 (en)
CN (1) CN104755626A (en)
AU (1) AU2013301368A1 (en)
BR (1) BR112015002682A2 (en)
GR (1) GR20120100415A (en)
IN (1) IN2015DN01760A (en)
MX (1) MX2015001717A (en)
WO (1) WO2014023984A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105717310A (en) * 2016-04-15 2016-06-29 肖乐义 Immuno-fluorescent staining method for detecting mycobacterium tuberculosis in leukocytes and kit
CN105759038A (en) * 2016-04-15 2016-07-13 肖乐义 Immunoassay method and kit for assaying mycobacterium tuberculosis from biological samples
GR1009115B (en) * 2016-04-22 2017-09-14 Α. & Χ. Υφαντης Α.Β.Ε.Ε Ανωνυμος Βιομηχανικη Και Εμπορικη Εταιρεια Foodstuff consisted of meat substitutes
ES2950436T3 (en) 2016-12-14 2023-10-10 Becton Dickinson Co Methods and compositions to obtain an assessment of tuberculosis in a subject
US11131618B2 (en) 2018-08-10 2021-09-28 Cytek Biosciences, Inc. Smart flow cytometers with self monitoring and self validation
CN109813903A (en) * 2019-01-29 2019-05-28 浙江省人民医院 The method for being used for diagnosis of tuberculosis mycobacterial infections based on Flow cytometry tuberculosis Specific T cell immunity phenotype

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT1350839E (en) * 2002-04-05 2010-08-12 Pasteur Institut Identification of virulence associated regions rd1 and rd5 enabling the development of improved vaccines of m. bovis bcg and m. microti
EP1674868A1 (en) * 2004-12-21 2006-06-28 Chang Gung University (a university of Taiwan) Method and device for detection of mycobacterium tuberculosis antigens in biological fluids
EP2335721A1 (en) * 2009-12-21 2011-06-22 Institut Pasteur Streptavidin and Biotin-based antigen delivery system
WO2011138765A1 (en) * 2010-05-06 2011-11-10 University Of The Witwatersrand, Johannesburg A method for identifying bacteria in a sample

Also Published As

Publication number Publication date
WO2014023984A2 (en) 2014-02-13
IN2015DN01760A (en) 2015-05-29
US20150204885A1 (en) 2015-07-23
BR112015002682A2 (en) 2017-07-04
CN104755626A (en) 2015-07-01
EP2882861A2 (en) 2015-06-17
MX2015001717A (en) 2015-12-03
GR1008136B (en) 2014-03-10
WO2014023984A3 (en) 2014-04-03
AU2013301368A1 (en) 2015-02-26

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Legal Events

Date Code Title Description
PG Patent granted

Effective date: 20140416