GR1007801B - Ακινητοποιηση λιπιδικων υποστρωματων με σκοπο την αναπτυξη και αξιολογηση φθορισμομετρικης μεθοδου προσδιορισμου ενεργοτητας φωσφολιπασης α2 σε βιολογικα δειγματα - Google Patents

Ακινητοποιηση λιπιδικων υποστρωματων με σκοπο την αναπτυξη και αξιολογηση φθορισμομετρικης μεθοδου προσδιορισμου ενεργοτητας φωσφολιπασης α2 σε βιολογικα δειγματα

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Publication number
GR1007801B
GR1007801B GR20110100718A GR20110100718A GR1007801B GR 1007801 B GR1007801 B GR 1007801B GR 20110100718 A GR20110100718 A GR 20110100718A GR 20110100718 A GR20110100718 A GR 20110100718A GR 1007801 B GR1007801 B GR 1007801B
Authority
GR
Greece
Prior art keywords
immobilization
activity
phospholipase
biological samples
order
Prior art date
Application number
GR20110100718A
Other languages
English (en)
Inventor
Μαρια-Ελενη Ευαγγελου Λεκκα
Αθανασιος Κωνσταντινου Καρκαμπουνας
Original Assignee
Επιτροπη Ερευνων Πανεπιστημιου Ιωαννινων (Ε.Λ.Κ.Ε.),
Μαρια-Ελενη Ευαγγελου Λεκκα
Αθανασιος Κωνσταντινου Καρκαμπουνας
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Επιτροπη Ερευνων Πανεπιστημιου Ιωαννινων (Ε.Λ.Κ.Ε.),, Μαρια-Ελενη Ευαγγελου Λεκκα, Αθανασιος Κωνσταντινου Καρκαμπουνας filed Critical Επιτροπη Ερευνων Πανεπιστημιου Ιωαννινων (Ε.Λ.Κ.Ε.),
Priority to GR20110100718A priority Critical patent/GR1007801B/el
Publication of GR1007801B publication Critical patent/GR1007801B/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Ο σκοπός της παρούσας εφεύρεσης αναφέρεται αρχικά στην ακινητοποίηση λιπιδικών υποστρωμάτων, που περιέχουν μία φθορίζουσα ομάδα στο μόριό τους, και ακολούθως στη μελέτη των μορίων αυτών με σκοπό την ανίχνευση και τον φθορισμομετρικό προσδιορισμό Φωσφολιπάσης Α2 σε βιολογικά δείγματα. Ειδικότερα, η παρούσα εφεύρεση αναφέρεται στην ακινητοποίηση υποστρωμάτων, που περιέχουν την 7-νιτρο-2-1,3-βενζοξοδιαζολο-4-υλο φθορίζουσα ομάδα στο μόριό τους, σε αδρανές υλικό και εν συνεχεία στη χρήση των ακινητοποιημένων αυτών υποστρωμάτων για τον φθορισμομετρικό προσδιορισμό ενεργότητας Φωσφολιπάσης Α2 σε βιολογικά δείγματα.
GR20110100718A 2011-12-20 2011-12-20 Ακινητοποιηση λιπιδικων υποστρωματων με σκοπο την αναπτυξη και αξιολογηση φθορισμομετρικης μεθοδου προσδιορισμου ενεργοτητας φωσφολιπασης α2 σε βιολογικα δειγματα GR1007801B (el)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20110100718A GR1007801B (el) 2011-12-20 2011-12-20 Ακινητοποιηση λιπιδικων υποστρωματων με σκοπο την αναπτυξη και αξιολογηση φθορισμομετρικης μεθοδου προσδιορισμου ενεργοτητας φωσφολιπασης α2 σε βιολογικα δειγματα

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20110100718A GR1007801B (el) 2011-12-20 2011-12-20 Ακινητοποιηση λιπιδικων υποστρωματων με σκοπο την αναπτυξη και αξιολογηση φθορισμομετρικης μεθοδου προσδιορισμου ενεργοτητας φωσφολιπασης α2 σε βιολογικα δειγματα

Publications (1)

Publication Number Publication Date
GR1007801B true GR1007801B (el) 2013-01-18

Family

ID=46584063

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20110100718A GR1007801B (el) 2011-12-20 2011-12-20 Ακινητοποιηση λιπιδικων υποστρωματων με σκοπο την αναπτυξη και αξιολογηση φθορισμομετρικης μεθοδου προσδιορισμου ενεργοτητας φωσφολιπασης α2 σε βιολογικα δειγματα

Country Status (1)

Country Link
GR (1) GR1007801B (el)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11396499B2 (en) 2018-12-12 2022-07-26 University Of Washington Lysosomal acid lipase assay

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0245799A2 (en) * 1986-05-13 1987-11-19 Terumo Kabushiki Kaisha Electromagnetic wave-sensitive material and bio-adaptable surface treating agent
WO2006085009A1 (fr) * 2005-02-11 2006-08-17 Centre National De La Recherche Scientifique Procede de detection et/ou de mesure a haut debit d’une activite lipasique ou phospholipasique
WO2010092068A1 (en) * 2009-02-10 2010-08-19 Aterovax Enzymatic assay for the quantitative determination of phospholipase a1 or a2 activity in a sample

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0245799A2 (en) * 1986-05-13 1987-11-19 Terumo Kabushiki Kaisha Electromagnetic wave-sensitive material and bio-adaptable surface treating agent
WO2006085009A1 (fr) * 2005-02-11 2006-08-17 Centre National De La Recherche Scientifique Procede de detection et/ou de mesure a haut debit d’une activite lipasique ou phospholipasique
WO2010092068A1 (en) * 2009-02-10 2010-08-19 Aterovax Enzymatic assay for the quantitative determination of phospholipase a1 or a2 activity in a sample

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A. KARKABOUNAS ET AL: "HPLC-fluorimetric assay of phospholipase A2. Application to biological samples with high protein content and various reaction conditions", JOURNAL OF CHROMATOGRAPHY B, vol. 879, no. 19, 1 June 2011 (2011-06-01), pages 1557 - 1564, XP055036056, ISSN: 1570-0232, DOI: 10.1016/j.jchromb.2011.03.047 *
KITSIOULI ET AL: "Differential determination of phospholipase A(2) and PAF-acetylhydrolase in biological fluids using fluorescent substrates.", THE JOURNAL OF LIPID RESEARCH, vol. 40, no. 12, 1 December 1999 (1999-12-01), pages 2346 - 2356, XP055036060, ISSN: 0022-2275 *
LETICIA HOSTA-RIGAU ET AL: "Capsosomes with "Free-Floating" Liposomal Subcompartments", ADVANCED MATERIALS, vol. 23, no. 35, 15 September 2011 (2011-09-15), pages 4082 - 4087, XP055036295, ISSN: 0935-9648, DOI: 10.1002/adma.201100908 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11396499B2 (en) 2018-12-12 2022-07-26 University Of Washington Lysosomal acid lipase assay

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Effective date: 20130225