GR1007617B - Use of non-linear imaging techniques in the evaluation of pre-implantation embryo quality and the facilitation of a successful pregnancy - Google Patents

Use of non-linear imaging techniques in the evaluation of pre-implantation embryo quality and the facilitation of a successful pregnancy

Info

Publication number
GR1007617B
GR1007617B GR20110100030A GR20110100030A GR1007617B GR 1007617 B GR1007617 B GR 1007617B GR 20110100030 A GR20110100030 A GR 20110100030A GR 20110100030 A GR20110100030 A GR 20110100030A GR 1007617 B GR1007617 B GR 1007617B
Authority
GR
Greece
Prior art keywords
embryo
imaging techniques
energetic
successful pregnancy
implantation
Prior art date
Application number
GR20110100030A
Other languages
Greek (el)
Inventor
Κωνσταντινος Εμμανουηλ Φωτακης
Γεωργιος Μηνα Φιλιππιδης
Ανθουλα Ελευθεριου Ρανελλα
Γεωργιος Ιωαννη Τσερεβελακης
Ειρηνη Ηλια Αθανασακη
Χριστιανα Ελευθεριου Κυβελιδου
Original Assignee
Ιδρυμα Τεχνολογιας Και Ερευνας - Ιτε,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ιδρυμα Τεχνολογιας Και Ερευνας - Ιτε, filed Critical Ιδρυμα Τεχνολογιας Και Ερευνας - Ιτε,
Priority to GR20110100030A priority Critical patent/GR1007617B/en
Publication of GR1007617B publication Critical patent/GR1007617B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0604—Whole embryos; Culture medium therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications

Abstract

Provided herein are methods to evaluate pre-implantation mouse embryo health and consequently allow embryo selection for transfer in the uterus and successful pregnancy outcome. Non linear (Two Photon Excitation Fluorescence (TPEF) Second and Third Harmonic Generation (SHG, and THG)) imaging techniques have been used as diagnostic tools for the in vivo delineation and mapping of sub-cellular biological structures and processes. By employing these non linear imaging modalities, valuable and complementary information as to the energetic status of the pre-implantation embryo are being extracted. The energetic profile of the embryo is directly correlated to the developmental stage of the embryo. Each time point of every developmental stage is being characterized by a specific energetic profile which can be recorded in a data bank and provide information as to the health status of the embryo.
GR20110100030A 2011-01-24 2011-01-24 Use of non-linear imaging techniques in the evaluation of pre-implantation embryo quality and the facilitation of a successful pregnancy GR1007617B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20110100030A GR1007617B (en) 2011-01-24 2011-01-24 Use of non-linear imaging techniques in the evaluation of pre-implantation embryo quality and the facilitation of a successful pregnancy

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20110100030A GR1007617B (en) 2011-01-24 2011-01-24 Use of non-linear imaging techniques in the evaluation of pre-implantation embryo quality and the facilitation of a successful pregnancy

Publications (1)

Publication Number Publication Date
GR1007617B true GR1007617B (en) 2012-06-20

Family

ID=44883311

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20110100030A GR1007617B (en) 2011-01-24 2011-01-24 Use of non-linear imaging techniques in the evaluation of pre-implantation embryo quality and the facilitation of a successful pregnancy

Country Status (1)

Country Link
GR (1) GR1007617B (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100041090A1 (en) * 2006-06-16 2010-02-18 Unisense Fertilitech A/S Embryo quality assessment based on blastomere division and movement

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100041090A1 (en) * 2006-06-16 2010-02-18 Unisense Fertilitech A/S Embryo quality assessment based on blastomere division and movement

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AVILES-ESPINOSA RODRIGO ET AL: "Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis", JOURNAL OF BIOMEDICAL OPTICS, vol. 15, no. 4, July 2010 (2010-07-01), XP002668017, ISSN: 1083-3668 *
CHEN SZU-YU ET AL: "Noninvasive harmonics optical microscopy for long-term observation of embryonic nervous system development in vivo", JOURNAL OF BIOMEDICAL OPTICS, vol. 11, no. 5, September 2006 (2006-09-01), XP002668014, ISSN: 1083-3668 *
SUN CHI-KUANG ET AL: "Higher harmonic generation microscopy for developmental biology", JOURNAL OF STRUCTURAL BIOLOGY, vol. 147, no. 1, July 2004 (2004-07-01), pages 19 - 30, XP002668015, ISSN: 1047-8477 *
TSEREVELAKIS G J ET AL: "Imaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy", MICRON, vol. 41, no. 5, July 2010 (2010-07-01), pages 444 - 447, XP002668016, ISSN: 0968-4328 *

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Effective date: 20120713