GR1005561B - Measurement of the total antioxidant capacity in liquids and solutions by using of 3,3', 5,5', tetramethylbenzidine - Google Patents

Measurement of the total antioxidant capacity in liquids and solutions by using of 3,3', 5,5', tetramethylbenzidine

Info

Publication number
GR1005561B
GR1005561B GR20050100503A GR20050100503A GR1005561B GR 1005561 B GR1005561 B GR 1005561B GR 20050100503 A GR20050100503 A GR 20050100503A GR 20050100503 A GR20050100503 A GR 20050100503A GR 1005561 B GR1005561 B GR 1005561B
Authority
GR
Greece
Prior art keywords
antioxidant capacity
measurement
total antioxidant
tetramethylbenzidine
liquids
Prior art date
Application number
GR20050100503A
Other languages
Greek (el)
Other versions
GR20050100503A (en
Inventor
Alamdari Daryoush Hamidi
Γεωργιος Κολιακος
Original Assignee
Γεωργιος Κολιακος
Alamdari Daryoush Hamidi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Γεωργιος Κολιακος, Alamdari Daryoush Hamidi filed Critical Γεωργιος Κολιακος
Priority to GR20050100503A priority Critical patent/GR1005561B/en
Priority to PCT/GR2006/000053 priority patent/WO2007039775A1/en
Publication of GR20050100503A publication Critical patent/GR20050100503A/en
Publication of GR1005561B publication Critical patent/GR1005561B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

An analytical chemical method that can be used for the measurement of the total antioxidant capacity of an organic or inorganic liquid is described. The antioxidant capacity is determined by the decolourization of 3,3; 5,5; - Tetramethyl benzidine (TMB) cation exerted by the antioxidants of the sample. TMB cation will be decolourized by reduction in a redox reaction. The quantitative analysis of the total antioxidant capacity of the sample can be easily made by spectrophotometry at 450 nm (reference wavelenght 620 or 570 nm) both by macro-and micromethods (ELISA reader) with the use of a number of reducing standard compositions with known concentrations.
GR20050100503A 2005-10-03 2005-10-03 Measurement of the total antioxidant capacity in liquids and solutions by using of 3,3', 5,5', tetramethylbenzidine GR1005561B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GR20050100503A GR1005561B (en) 2005-10-03 2005-10-03 Measurement of the total antioxidant capacity in liquids and solutions by using of 3,3', 5,5', tetramethylbenzidine
PCT/GR2006/000053 WO2007039775A1 (en) 2005-10-03 2006-10-03 Measurement of the total antioxidant capacity in liquids and solutions using tmb

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20050100503A GR1005561B (en) 2005-10-03 2005-10-03 Measurement of the total antioxidant capacity in liquids and solutions by using of 3,3', 5,5', tetramethylbenzidine

Publications (2)

Publication Number Publication Date
GR20050100503A GR20050100503A (en) 2007-05-23
GR1005561B true GR1005561B (en) 2007-06-15

Family

ID=37309652

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20050100503A GR1005561B (en) 2005-10-03 2005-10-03 Measurement of the total antioxidant capacity in liquids and solutions by using of 3,3', 5,5', tetramethylbenzidine

Country Status (2)

Country Link
GR (1) GR1005561B (en)
WO (1) WO2007039775A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8709709B2 (en) 2007-05-18 2014-04-29 Luoxis Diagnostics, Inc. Measurement and uses of oxidative status
ES2627188T3 (en) 2007-05-18 2017-07-27 Aytu Bioscience, Inc. Measurement and uses of the oxidative state
CN103299181B (en) 2011-02-28 2015-04-08 卢奥克西斯诊断股份有限公司 Method and apparatus for measuring redox potential
WO2013158985A1 (en) 2012-04-19 2013-10-24 Luoxis Diagnostics, Inc. Multiple layer gel
US9410913B2 (en) 2012-10-23 2016-08-09 Aytu Bioscience, Inc. Methods and systems for measuring and using the oxidation-reduction potential of a biological sample
IT201800003475A1 (en) * 2018-03-13 2019-09-13 Fondazione St Italiano Tecnologia Procedure for determining the antioxidant capacity of a biological sample and relative kit.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9027131D0 (en) * 1990-12-14 1991-02-06 Rice Evans Catherine Diagnostic test

Also Published As

Publication number Publication date
GR20050100503A (en) 2007-05-23
WO2007039775A1 (en) 2007-04-12

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Legal Events

Date Code Title Description
PG Patent granted
ML Lapse due to non-payment of fees

Effective date: 20110503