GR1003896B - New method of isolating total rna from paraffin-embedded tissues - Google Patents

New method of isolating total rna from paraffin-embedded tissues

Info

Publication number
GR1003896B
GR1003896B GR20010100118A GR2001100118A GR1003896B GR 1003896 B GR1003896 B GR 1003896B GR 20010100118 A GR20010100118 A GR 20010100118A GR 2001100118 A GR2001100118 A GR 2001100118A GR 1003896 B GR1003896 B GR 1003896B
Authority
GR
Greece
Prior art keywords
total rna
paraffin
embedded tissues
isolation
new method
Prior art date
Application number
GR20010100118A
Other languages
Greek (el)
Inventor
Γεωργακοπουλος Ελευθεριος Δρ.
Original Assignee
Γεωργακοπουλος Ελευθεριος Δρ.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Γεωργακοπουλος Ελευθεριος Δρ. filed Critical Γεωργακοπουλος Ελευθεριος Δρ.
Priority to GR20010100118A priority Critical patent/GR1003896B/en
Publication of GR1003896B publication Critical patent/GR1003896B/en

Links

Landscapes

  • Saccharide Compounds (AREA)

Abstract

he total RNA isolation from paraffin-embedded tissues, independently from the duration of the paraffinization process, is of a particular importance for, inter alia, the clinical activity; it will be predominantly helpful to the continual exploitation of clinical specimens used for the follow-up and evaluation of the clinical condition of patients. The technique used by the invention utilizes commercial solutions, and is based on the tissue hydration with the solution referred therein, any other hydration process being excluded. The isolation of the total RNA is certified with the use of photometric analysis or/and PCR chain reaction. The new method consists in the isolation of total RNA from paraffin-embedded tissues, independently from the duration of the paraffinization process, in a rate of 100% and in concentrations reaching the 1mg/ml of total RNA.
GR20010100118A 2001-03-13 2001-03-13 New method of isolating total rna from paraffin-embedded tissues GR1003896B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20010100118A GR1003896B (en) 2001-03-13 2001-03-13 New method of isolating total rna from paraffin-embedded tissues

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20010100118A GR1003896B (en) 2001-03-13 2001-03-13 New method of isolating total rna from paraffin-embedded tissues

Publications (1)

Publication Number Publication Date
GR1003896B true GR1003896B (en) 2002-05-23

Family

ID=10944676

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20010100118A GR1003896B (en) 2001-03-13 2001-03-13 New method of isolating total rna from paraffin-embedded tissues

Country Status (1)

Country Link
GR (1) GR1003896B (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0953635A1 (en) * 1998-04-28 1999-11-03 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0953635A1 (en) * 1998-04-28 1999-11-03 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DENNINGHOFF V C ET AL: "Detection and RT-PCR amplification of RNA from paraffin-embedded lymph nodes.", LABORATORY INVESTIGATION, vol. 81, no. 1, January 2001 (2001-01-01), Annual Meeting of the United States and Canadian Academy of Pathology;Atlanta, Georgia, USA; March 03-09, 2001, pages 232A, XP002190015, ISSN: 0023-6837 *
FOSS R D ET AL: "EFFECTS OF FIXATIVE AND FIXATION TIME ON THE EXTRACTION AND POLYMERASE CHAIN REACTION AMPLIFICATION OF RNA FROM PARAFFIN-EMBEDDED TISSUE COMPARISON OF TWO HOUSEKEEPING GENE MRNA CONTROLS", DIAGNOSTIC MOLECULAR PATHOLOGY, NEW YORK, NY, US, vol. 3, no. 3, 1 September 1994 (1994-09-01), pages 148 - 155, XP000575426 *
KOOPMANS M ET AL: "OPTIMIZATION OF EXTRACTION AND PCR AMPLIFICATION OF RNA EXTRACTS FROM PARAFFIN-EMBEDDED TISSUE IN DIFFERENT FIXATIVES", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 43, no. 2, 1993, pages 189 - 204, XP000997425, ISSN: 0166-0934 *
SPECHT KATJA ET AL: "Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue.", AMERICAN JOURNAL OF PATHOLOGY, vol. 158, no. 2, February 2001 (2001-02-01), pages 419 - 429, XP001053519, ISSN: 0002-9440 *

Similar Documents

Publication Publication Date Title
Reibnegger et al. Predictive value of interleukin-6 and neopterin in patients with multiple myeloma
MXPA02006145A (en) Method for isolation of rna from formalinfixed paraffinembedded tissue specimens.
MXPA02007421A (en) Devices for analyte concentration determination and methods of using the same.
CA2354379A1 (en) Method for isolating dna from biological materials
AU9058091A (en) Preparation of nucleic acid samples
WO2002083945A3 (en) Diagnosis and treatment of cancer: i
WO2002031507A3 (en) Non-invasive enzyme screen for tissue remodelling-associated conditions
MY134005A (en) Diagnostic based on tetrazolium compounds
UA86582C2 (en) Method for treatment of huntington's chorea with eicosapentaenoic acid
AU2002317557A1 (en) Afinity biosensor for monitoring of biological process
WO2004044247A3 (en) One step real-time rt pcr kits for the universal detection of organisms in industrial products
AU5502101A (en) Cancer diagnosis method
NO20063939L (en) Oxidizable species as an internal reference for biosensors and methods of use
WO2003016464A3 (en) Cancer specific oligosaccharide sequences and use thereof
WO2005085863A3 (en) Biomarker: compound correlations in cancer diagnosis and therapy
CA2468877A1 (en) A method for determining the biological activity of defibrotide
BR9906926A (en) Compositions and methods for treating cells having double minute dna
WO2005054516A3 (en) Peptide nucleic acid probes for analysis of certain staphylococcus species
WO2006124866A3 (en) Free human serum ige immunoenzymetric assay and methods of use
GR1003896B (en) New method of isolating total rna from paraffin-embedded tissues
AU2003250938A1 (en) Method for determination of thymidine kinase 1 activity and the use thereof
WO2002059611A3 (en) Diagnosis and treatment of cancer using mammalian pellino polypeptides and polynucleotides
WO2005040754A3 (en) METHOD AND KIT FOR DETECTING LISTERIA spp.
WO2007001943A3 (en) Prefoldin 4 in the treatment and diagnosis of cancer
WO2003014394A1 (en) Method of evaluating biocompatibility