GB978743A - Process for the preparation of an eosinophil-containing material and extracts thereof from blood - Google Patents
Process for the preparation of an eosinophil-containing material and extracts thereof from bloodInfo
- Publication number
- GB978743A GB978743A GB18581/60A GB1858160A GB978743A GB 978743 A GB978743 A GB 978743A GB 18581/60 A GB18581/60 A GB 18581/60A GB 1858160 A GB1858160 A GB 1858160A GB 978743 A GB978743 A GB 978743A
- Authority
- GB
- United Kingdom
- Prior art keywords
- eosinophil
- blood
- containing material
- erythrocytes
- sediment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000003979 eosinophil Anatomy 0.000 title abstract 5
- 210000004369 blood Anatomy 0.000 title abstract 3
- 239000008280 blood Substances 0.000 title abstract 3
- 239000000463 material Substances 0.000 title abstract 3
- 239000000284 extract Substances 0.000 title 1
- 210000003743 erythrocyte Anatomy 0.000 abstract 2
- 239000013049 sediment Substances 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 230000001387 anti-histamine Effects 0.000 abstract 1
- 230000003110 anti-inflammatory effect Effects 0.000 abstract 1
- 239000000739 antihistaminic agent Substances 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000002934 lysing effect Effects 0.000 abstract 1
- 238000004062 sedimentation Methods 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 238000010257 thawing Methods 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
Landscapes
- Health & Medical Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- External Artificial Organs (AREA)
Abstract
Eosinophil leucocytes are extracted from mammalian blood, e.g of a horse or human, by centrifuging the blood, optionally after initial sedimentation to remove the bulk of the erythrocytes, discarding the serum and "buffy coat layer" of the sediment and separating the eosinophils or an eosinophil-containing material from the remaining heavier fraction of the sediment, e.g. by lysing the accompanying erythrocytes by addition of saline and recentrifuging. The eosinophils obtained may be disrupted, e.g. by use of a homogenizing mill, preferably after freezing and thawing, or by ultra-sonic disruption to give a material which may be freeze-dried and which has antihistaminic and possibly general anti-inflammatory activity.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB18581/60A GB978743A (en) | 1960-05-25 | 1960-05-25 | Process for the preparation of an eosinophil-containing material and extracts thereof from blood |
| US111620A US3170838A (en) | 1960-05-25 | 1961-05-22 | Centrifugation of whole blood to separate eosinophils |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB18581/60A GB978743A (en) | 1960-05-25 | 1960-05-25 | Process for the preparation of an eosinophil-containing material and extracts thereof from blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB978743A true GB978743A (en) | 1964-12-23 |
Family
ID=10114912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB18581/60A Expired GB978743A (en) | 1960-05-25 | 1960-05-25 | Process for the preparation of an eosinophil-containing material and extracts thereof from blood |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US3170838A (en) |
| GB (1) | GB978743A (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL109202C (en) * | 1959-11-20 | |||
| US3814522A (en) * | 1973-02-28 | 1974-06-04 | American Hospital Supply Corp | Specimen tube for microscopic examination |
| US3914985A (en) * | 1974-03-29 | 1975-10-28 | American Hospital Supply Corp | Centrifuging device and method |
| US3946941A (en) * | 1974-09-25 | 1976-03-30 | Gunther Weiss | Centrifuging apparatus |
| US4004975A (en) * | 1975-12-30 | 1977-01-25 | The United States Of America As Represented By The Secretary Of The Navy | Method of isolating and cryopreserving human white cells from whole blood |
| US4027660A (en) * | 1976-04-02 | 1977-06-07 | Wardlaw Stephen C | Material layer volume determination |
| US4105415A (en) * | 1976-04-21 | 1978-08-08 | Lovett Wayne D | Multi-purpose test tube |
| US4344562A (en) * | 1979-02-01 | 1982-08-17 | Andrew Ricci | Centrifuge cell collector apparatus |
| DE3009126A1 (en) * | 1980-03-10 | 1981-10-08 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | METHOD FOR OBTAINING FUNCTIONAL AND MORPHOLOGICALLY INTACT AND VIABLE LEUCOCYTES FROM BLOOD |
-
1960
- 1960-05-25 GB GB18581/60A patent/GB978743A/en not_active Expired
-
1961
- 1961-05-22 US US111620A patent/US3170838A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US3170838A (en) | 1965-02-23 |
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