GB9418438D0 - A method for improving reaction stringency when replicating and/or amplifying a nucleic acid sequence - Google Patents

A method for improving reaction stringency when replicating and/or amplifying a nucleic acid sequence

Info

Publication number
GB9418438D0
GB9418438D0 GB9418438A GB9418438A GB9418438D0 GB 9418438 D0 GB9418438 D0 GB 9418438D0 GB 9418438 A GB9418438 A GB 9418438A GB 9418438 A GB9418438 A GB 9418438A GB 9418438 D0 GB9418438 D0 GB 9418438D0
Authority
GB
United Kingdom
Prior art keywords
replicating
amplifying
nucleic acid
acid sequence
improving reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB9418438A
Other versions
GB2293238A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RAY RONNIE A
Original Assignee
RAY RONNIE A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by RAY RONNIE A filed Critical RAY RONNIE A
Priority to GB9418438A priority Critical patent/GB2293238A/en
Publication of GB9418438D0 publication Critical patent/GB9418438D0/en
Publication of GB2293238A publication Critical patent/GB2293238A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB9418438A 1994-09-13 1994-09-13 Primers for replication and/or amplification reactions Withdrawn GB2293238A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9418438A GB2293238A (en) 1994-09-13 1994-09-13 Primers for replication and/or amplification reactions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9418438A GB2293238A (en) 1994-09-13 1994-09-13 Primers for replication and/or amplification reactions

Publications (2)

Publication Number Publication Date
GB9418438D0 true GB9418438D0 (en) 1994-11-02
GB2293238A GB2293238A (en) 1996-03-20

Family

ID=10761254

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9418438A Withdrawn GB2293238A (en) 1994-09-13 1994-09-13 Primers for replication and/or amplification reactions

Country Status (1)

Country Link
GB (1) GB2293238A (en)

Families Citing this family (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
FR2779154B1 (en) * 1998-05-27 2002-07-12 Bio Merieux METHOD FOR AMPLIFYING AT LEAST ONE PARTICULAR NUCLEOTIDE SEQUENCE AND PRIMES FOR IMPLEMENTATION
AU759915B2 (en) 1998-07-31 2003-05-01 Gen-Probe Incorporated Reversible inhibiting probes
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6187567B1 (en) * 1999-09-01 2001-02-13 Akzo Nobel N.V. Methods and reagents for in situ amplification
DE10012540B4 (en) * 2000-03-15 2004-09-23 Vermicon Ag Oligonucleotides and methods for the specific detection of microorganisms by polymerase chain reaction
US6248567B1 (en) * 2000-04-24 2001-06-19 Wisconsin Alumni Research Foundation Template-specific termination in a polymerase chain reaction
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
EP1253205A1 (en) * 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
EP1277841B1 (en) * 2001-07-11 2009-08-26 Roche Diagnostics GmbH New composition and method for hot start nucleic acid amplification
AU2003213107A1 (en) 2002-02-15 2003-09-09 Exact Sciences Corporation Methods for analysis of molecular events
AU2003224141A1 (en) * 2002-04-30 2003-11-17 Gnothis Holding Sa Multiplexed determination of nucleic acid polymorphisms
CA2522753C (en) 2003-04-18 2014-06-10 Becton, Dickinson And Company Immuno-amplification
US7338763B2 (en) 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
WO2007044071A2 (en) 2005-04-21 2007-04-19 Exact Sciences Corporation Analysis of heterogeneous nucleic acid samples
DE102005047617A1 (en) * 2005-10-05 2007-04-19 Qiagen Gmbh Polymerase chain reaction method using a DNA polymerase with proofreading property
CA2638854C (en) 2006-02-27 2012-05-15 F. Hoffmann-La Roche Ag Pcr hot start by magnesium sequestration
CA2658520C (en) 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
ES2665500T3 (en) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enrichment of a complete COLD PCR with reference blocking sequence
GB201100150D0 (en) 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
CN103517993A (en) * 2011-02-28 2014-01-15 环球基因有限公司 Kit and method for sequencing a target DNA in a mixed population
EP2691541B1 (en) 2011-03-31 2017-10-18 Dana-Farber Cancer Institute, Inc. Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences
EP2570487A1 (en) 2011-09-16 2013-03-20 Lexogen GmbH Nucleic acid transcription method
WO2013038010A2 (en) 2011-09-16 2013-03-21 Lexogen Gmbh Nucleic acid transcription method
WO2013097173A1 (en) * 2011-12-30 2013-07-04 Mackay Memorial Hospital Method and primer set for detecting mutation
JP6375230B2 (en) 2012-02-27 2018-08-15 セルラー リサーチ， インコーポレイテッド Compositions and kits for molecular counting
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
KR20230074639A (en) 2013-08-28 2023-05-30 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US11535882B2 (en) 2015-03-30 2022-12-27 Becton, Dickinson And Company Methods and compositions for combinatorial barcoding
CN107580632B (en) 2015-04-23 2021-12-28 贝克顿迪金森公司 Methods and compositions for whole transcriptome amplification
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド Methods and compositions for library normalization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR102363716B1 (en) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. Determination of protein expression using reagents having barcoded oligonucleotide sequences
EP3551756A4 (en) 2016-12-12 2020-07-15 Dana Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
JP6933907B2 (en) * 2017-02-24 2021-09-08 シスメックス株式会社 Nucleic acid amplification method
EP4345172A2 (en) 2017-06-05 2024-04-03 Becton, Dickinson and Company Sample indexing for single cells
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. Methods and compositions for selecting and amplifying dna targets in a single reaction mixture
US11365409B2 (en) 2018-05-03 2022-06-21 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
CN112272710A (en) 2018-05-03 2021-01-26 贝克顿迪金森公司 High throughput omics sample analysis
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
WO2020123384A1 (en) 2018-12-13 2020-06-18 Cellular Research, Inc. Selective extension in single cell whole transcriptome analysis
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins

Also Published As

Publication number Publication date
GB2293238A (en) 1996-03-20

Similar Documents

Publication Publication Date Title
GB9418438D0 (en) A method for improving reaction stringency when replicating and/or amplifying a nucleic acid sequence
IL120865A (en) Method for amplifying a nucleic acid sequence and kit therefor
EP0674009A3 (en) Nucleic acid amplification method and apparatus.
HUP9602289A2 (en) Method for amplifying nucleic acid
EP0733714A3 (en) Nucleic acid amplification method and apparatus
EP0757106A3 (en) Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same
AU2929495A (en) Nucleic acid sequence amplification method
AU3959095A (en) A method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (pna)
IL121550A0 (en) System and method for handling permits
HK1027228A1 (en) Method and means for handling information
EP0693560A3 (en) Method and apparatus for fully automated nucleic acid amplification, nucleic acid assay and immunoassay
IL117884A0 (en) Method for amplifying specific nucleic acid sequences
AU3890795A (en) Improved method for nucleotide sequence amplification
HUP9801569A2 (en) Process for polymerizing and reactor
AU2704997A (en) Method for detecting a nucleic acid base sequence
AU3376997A (en) Method for amplifying a polynucleotide
AU3376897A (en) Microfluidic method for nucleic acid amplification
AU3752195A (en) Apparatus and method for storage, purification or reaction and processing of a biopolymer
ZA983382B (en) A method and culture for inhibiting undesired micro-organisms
HUP9600724A1 (en) Reagent and process for geting a substituated diflouromethyl group into a compound cointaining one or more electrophyl group
AU4508396A (en) Method for deslagging a partial oxidation reactor
IL105894A (en) Method for the detection of nucleic acid sequence by using a detection ensemble and an amplification ensemble
HK1033171A1 (en) Process for obtaining l-dihydroorotic acid and usethereof
GB9301776D0 (en) Method for detecting genomic variation
GB9520338D0 (en) A method for improving reaction stringency when replicating and/or amplifying a nucleic acid sequence or performing in situ hybridisation

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)