GB927259A - Enzyme recovery process - Google Patents

Enzyme recovery process

Info

Publication number
GB927259A
GB927259A GB2022361A GB2022361A GB927259A GB 927259 A GB927259 A GB 927259A GB 2022361 A GB2022361 A GB 2022361A GB 2022361 A GB2022361 A GB 2022361A GB 927259 A GB927259 A GB 927259A
Authority
GB
United Kingdom
Prior art keywords
ethanol
volume
catalase
cells
bring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2022361A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ROLAND FRANK BEERS
Original Assignee
ROLAND FRANK BEERS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ROLAND FRANK BEERS filed Critical ROLAND FRANK BEERS
Publication of GB927259A publication Critical patent/GB927259A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Catalase is recovered from the cells of Micrococcus lysodeikticus by lysing a suspension of the cells in a 0.5% to 2.0% solution of sodium chloride containing a lysing enzyme, precipitating the lysed cells and proteinaceous material by adding ethanol to the lysed suspension in such a proportion as to bring the concentration of ethanol to between 40 and 52% by volume (calculated at 95% by volume ethanol), separating the supernatant liquid, and adding ethanol to the separated liquid in such a proportion as to bring the concentration of ethanol to between 70 and 85% by volume (calculated as 95% by volume ethanol) to precipitate catalase and recovering the catalase. The pH of the sodium chloride solution is preferably maintained between 6.0 and 9.0, the preferred enzyme is lysozyme and the lysis is preferably carried out at 30 DEG to 45 DEG C. Specification 819,336 is referred to.
GB2022361A 1960-06-13 1961-06-05 Enzyme recovery process Expired GB927259A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3546460A 1960-06-13 1960-06-13

Publications (1)

Publication Number Publication Date
GB927259A true GB927259A (en) 1963-05-29

Family

ID=21882835

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2022361A Expired GB927259A (en) 1960-06-13 1961-06-05 Enzyme recovery process

Country Status (3)

Country Link
BE (1) BE604714A (en)
CH (1) CH400059A (en)
GB (1) GB927259A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0029766A1 (en) * 1979-11-05 1981-06-03 EASTMAN KODAK COMPANY (a New Jersey corporation) Method for the recovery of creatinine iminohydrolase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0029766A1 (en) * 1979-11-05 1981-06-03 EASTMAN KODAK COMPANY (a New Jersey corporation) Method for the recovery of creatinine iminohydrolase

Also Published As

Publication number Publication date
CH400059A (en) 1965-10-15
BE604714A (en) 1961-10-02

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