GB906212A - The antibiotic valacidin and its salts and methods for producing same - Google Patents
The antibiotic valacidin and its salts and methods for producing sameInfo
- Publication number
- GB906212A GB906212A GB27074/60A GB2707460A GB906212A GB 906212 A GB906212 A GB 906212A GB 27074/60 A GB27074/60 A GB 27074/60A GB 2707460 A GB2707460 A GB 2707460A GB 906212 A GB906212 A GB 906212A
- Authority
- GB
- United Kingdom
- Prior art keywords
- valacidin
- water
- aqueous
- antibiotic
- salts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
<PICT:0906212/IV (b)/1> A new antibiotic designated Valacidin is produced by cultivating a Valacidin-producing strain of Streptomcyes Lavendulae especially S. Lavendulae NRRL 2765 in an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic salts, preferably under submerged aerobic conditions. Specified sources of carbon are glucose, sucrose, starch, glycerine, molasses, sorbitol, dextrine, brown sugar, mannitol and corn steep solids. Specified sources of nitrogen are linseed meal, tankage, fish meal, corn meal, cotton seed meal, oatmeal, ground wheat, soy bean meal, beef extract, peptones (meat or soy) asparagine, agar casein and amino acid mixtures. Specified inorganic salts are those providing sodium, potassium, ammonium, calcium, magnesium sulphate, chloride, phosphate or nitrate ions. Trace elements may be added to the culture medium. Cultivation at 25-32 DEG C. for 2-5 days at an initial pH of 5.5-8.0 is preferred. The antibiotic valacidin is isolated from the clarified broth by (a) extraction with a water-immiscible polar organic solvent of which typical examples are ethyl or amyl acetate, chloroform, butyl alcohol, methyl isobutyl ketones, or di-ethylether or (b) adsorption on adsorbants or ion exchange resins e.g. carbon or "Permutit DR" (Registered Trade Mark). The solvent extracts are purified by (i) concentrating in vacuo, extracting the concentrate with a weak aqueous solution of a base e.g. sodium hydroxide and extracting the aqueous extract after acidification with a water-immiscible polar organic solvent, reextracting with aqueous base and acidifying to pH 3 to precipitate valacidin, (ii) precipitation by addition of a non-solvent e.g. petroleum ether or (iii) adsorption chromatography using silicic acid, alumina, "Floril" (Registered Trade Mark) or ion exchange resins as adsorbant and eluting with a water-immiscible polar organic solvent. Salts of valacidin e.g. the sodium, potassium, calcium, magnesium, tributylamine or diethanolamine salts are formed with organic and inorganic bases. Valacidin and its cationic salts are active against gram positive and gram negative bacteria including certain plant pathogens e.g. X. phaseoli and Alternaria solani. Valacidin is a red brown solid organic acid soluble in aqueous alkaline solutions and polar solvents; slightly soluble in lower alcohols; insoluble in non-polar solvents and aqueous acid solutions; melts with decomposition at about 261 DEG C.; stable in aqueous solution at pH 1.0-9.0; molecular weight 470-510; contains 60.01% carbon, 4.66% hydrogen, 10.80% nitrogen and 24.53% oxygen; empirical formula C26H24O8N4; Pka in dimethyl formamide; water solution (2 : 1) of 7.0; I.R. absorption maxima in mineral oil mull at 2.90, 2.98, 3.07, 5.74, 5.95, 6.09, 6.18, 6.27, 6.39, 6.46, 6.65, 7.13, 7.46, 7.80, 8.11, 9.10, 9.24, 9.33, 9.63, 9.98, 10.89, 11.61, 12.32, 13.4, 13.9 and 14.2 microns; U.V. absorption maxima at 375mm , 294mm and 246mm in methanol with 16,800; 27,100; and 40,400 as the respective extinction coefficients; and Rf values of 0.40 in butanol and water, 0.75 in butanol and water containing 2% p-toluene sulphonic acid monohydrate and 0.00 in methyl-isobutyl ketone and water containing 2% piperidine. Chemical tests show the presence of phenolic, methoxy and carboxy groups and valacidin gives positive Fehlings and permanganate tests for reducing groups, a positive Molisch test and a negative ninhydrin test. Valacidin is used as a preservative (see Group VI).ALSO:Compositions for preserving organic materials by external application and more particularly fluids used for preserving cadavers and animal specimens contain the antibiotic valacidin or a cationic salt thereof (see Group IV (b)). In an example, a formaldehyde embalming solution contains 1.0 mg. of valacidin or a cationic salt thereof. In the form of a dust or spray the compositions containing the antibiotic are applied as plant fungicides.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB27074/60A GB906212A (en) | 1960-08-04 | 1960-08-04 | The antibiotic valacidin and its salts and methods for producing same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB27074/60A GB906212A (en) | 1960-08-04 | 1960-08-04 | The antibiotic valacidin and its salts and methods for producing same |
Publications (1)
Publication Number | Publication Date |
---|---|
GB906212A true GB906212A (en) | 1962-09-19 |
Family
ID=10253736
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB27074/60A Expired GB906212A (en) | 1960-08-04 | 1960-08-04 | The antibiotic valacidin and its salts and methods for producing same |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB906212A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2418431A (en) * | 2004-09-27 | 2006-03-29 | Multigerm Uk Entpr Ltd | Metabolically active micro organisms and methods for their production |
-
1960
- 1960-08-04 GB GB27074/60A patent/GB906212A/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2418431A (en) * | 2004-09-27 | 2006-03-29 | Multigerm Uk Entpr Ltd | Metabolically active micro organisms and methods for their production |
US8551473B2 (en) | 2004-09-27 | 2013-10-08 | Multigerm Uk Enterprises Ltd. | Metabolically active micro organisms and methods for their production |
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