GB8923522D0 - Amplification in recombinant dna host cells - Google Patents

Amplification in recombinant dna host cells

Info

Publication number
GB8923522D0
GB8923522D0 GB898923522A GB8923522A GB8923522D0 GB 8923522 D0 GB8923522 D0 GB 8923522D0 GB 898923522 A GB898923522 A GB 898923522A GB 8923522 A GB8923522 A GB 8923522A GB 8923522 D0 GB8923522 D0 GB 8923522D0
Authority
GB
United Kingdom
Prior art keywords
amplification
host cells
recombinant dna
dna host
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB898923522A
Other versions
GB2237288A (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UCB Celltech Ltd
Original Assignee
Celltech R&D Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech R&D Ltd filed Critical Celltech R&D Ltd
Priority to GB8923522A priority Critical patent/GB2237288A/en
Publication of GB8923522D0 publication Critical patent/GB8923522D0/en
Publication of GB2237288A publication Critical patent/GB2237288A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
GB8923522A 1989-10-18 1989-10-18 Amplification of a heterologous gene in recombinant eukaryotic host cells Withdrawn GB2237288A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB8923522A GB2237288A (en) 1989-10-18 1989-10-18 Amplification of a heterologous gene in recombinant eukaryotic host cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB8923522A GB2237288A (en) 1989-10-18 1989-10-18 Amplification of a heterologous gene in recombinant eukaryotic host cells

Publications (2)

Publication Number Publication Date
GB8923522D0 true GB8923522D0 (en) 1989-12-06
GB2237288A GB2237288A (en) 1991-05-01

Family

ID=10664800

Family Applications (1)

Application Number Title Priority Date Filing Date
GB8923522A Withdrawn GB2237288A (en) 1989-10-18 1989-10-18 Amplification of a heterologous gene in recombinant eukaryotic host cells

Country Status (1)

Country Link
GB (1) GB2237288A (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
JP5687057B2 (en) 2007-07-09 2015-03-18 ジェネンテック， インコーポレイテッド Prevention of disulfide bond reduction during recombinant production of polypeptides.
KR101822205B1 (en) * 2009-08-11 2018-01-25 제넨테크, 인크. Production of proteins in glutamine-free cell culture media

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8601597D0 (en) * 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences

Also Published As

Publication number Publication date
GB2237288A (en) 1991-05-01

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)