GB887395A - A method of fractionating protein solutions - Google Patents

A method of fractionating protein solutions

Info

Publication number
GB887395A
GB887395A GB3628259A GB3628259A GB887395A GB 887395 A GB887395 A GB 887395A GB 3628259 A GB3628259 A GB 3628259A GB 3628259 A GB3628259 A GB 3628259A GB 887395 A GB887395 A GB 887395A
Authority
GB
United Kingdom
Prior art keywords
quinolyl
parts
amino
dihydrochloride
volume
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3628259A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to GB3628259A priority Critical patent/GB887395A/en
Publication of GB887395A publication Critical patent/GB887395A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/38—Nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/16—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D215/38—Nitrogen atoms
    • C07D215/42—Nitrogen atoms attached in position 4

Abstract

Protein fractions are obtained by adding to a protein solution a quinoline compound having the structural formula <FORM:0887395/IV (b)/1> wherein X represents a bivalent organic radical containing at least two nitrogen atoms, each of the quinolyl radicals being attached to a nitrogen atom of radical X; R1, R11, R2, R12, R3 and R13 represent hydrogen, lower alkyl or substituted or unsubsti uted amino groups, and A represents an anion; and removing part of the protein as a precipitate. Radical X may be -NH.CO.NH-, -NH.CO.CH2.NH-, -NH.CO.CH2.CO.NH-, or <FORM:0887395/IV (b)/2> The quinoline compound may be added in the form of a solution, e.g. an aqueous solution, having a concentration of from 0.1% to 1.0% by weight, e.g. about 0.4%, and having a pH from 1.9 to 5.7. Quinoline compounds specified are N,N1 - di - (2 - methyl - 4 - amino - quinolyl - 6)-ureido-dihydrochloride, or -dichloromethylate, N, N1 - di - (2 - methyl - 4 - amino - quinolyl - 6) - amino-acetic acid amide -dihydrochloride, -triamino-triazine-sulphate or -malonic acid diamide-dihydrochloride; N-(2-aminoquinolyl-6)-N1-quinolyl-ureido-dihydrochloride. The protein solution may be human or animal blood sera, pleural exudates, ascites and solutions of plant or vegetable origin. Substantially pure gamma-globulin may be isolated by addition of an amount of quinoline compound equivalent to 32 to 40 parts by volume, e.g. about 36 parts, of a 0.4% by weight aqueous solution of the compound per 10 parts by volume of human blood serum. A mixture of beta- and gamma-globulins may be isolated by the addition of an amount of quinoline compound equivalent to 24 to 32 parts by volume of a 0.4% by weight aqueous solution of the compound per 10 parts by volume of human blood serum. The addition of smaller amounts of the quinoline compounds makes possible the separation from the serum of a mixture consisting of albumin and alpha-, beta- and gamma-globulins. Anion A is usually monovalent or bivalent but others, e.g. the phosphate anions, may be used.ALSO:Serum fractions are obtained by adding to a solution of human or animal blood sera a quinoline compound having the structural formula <FORM:0887395/VI/1> wherein X represents a bivalent organic radical containing at least two nitrogen atoms, each of the quinolyl radicals being attached to a nitrogen atom of radical X; R1, R11, R2, R12, R3 and R13 represent hydrogen, lower alkyl or substituted or unsubstituted amino groups, and A represents an anion; and removing part of the serum as a precipitate. Radical X may be -NH.CO.NH-, -NH.CO.CH2.NH-, -NH.CO.CH2.CO.NH-, or <FORM:0887395/VI/2> The quinoline compound may be added in the form of a solution, e.g. an aqueous solution, having a concentration of from 0.1 to 1.0% by weight, e.g. about 0.4% and having a pH from 1.9 to 5.7. Anion A is usually monovalent or bivalent but others e.g. the phosphate ions, may be used. Quinoline compounds specified are N,N1 - di - (2 - methyl - 4 - amino - quinolyl - 6) - ureido-dihydrochloride or -dichloromethylate; N,N1 - di - (2 - methyl - 4 - amino - quinolyl - 6) - amino acetic acid amide-dihydrochloride, -triamino - triazine - sulphate or -malonic acid diamide - dihydrochloride; N - (2 - amino - quinolyl - 6) - N1 - quinolyl - ureido - dihydro - chloride. Substantially pure gamma globulin and a mixture of beta and gamma globulins may be isolated by addition of an amount of quinoline compound equivalent to 32 to 40 parts by volume, e.g. about 36 parts, and 24 to 32 parts by volume, respectively, of a 0.4% by weight aqueous solution of the compound per 10 parts by volume of human blood serum. The addition of smaller amounts of the quinoline compound makes possible the separation from the serum of a mixture consisting of albumin and alpha-, beta- and gamma-globulins. Protein fractions may also be obtained by addition of the quinoline compound to pleural exudates or ascites.
GB3628259A 1959-10-26 1959-10-26 A method of fractionating protein solutions Expired GB887395A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB3628259A GB887395A (en) 1959-10-26 1959-10-26 A method of fractionating protein solutions

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3628259A GB887395A (en) 1959-10-26 1959-10-26 A method of fractionating protein solutions

Publications (1)

Publication Number Publication Date
GB887395A true GB887395A (en) 1962-01-17

Family

ID=10386683

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3628259A Expired GB887395A (en) 1959-10-26 1959-10-26 A method of fractionating protein solutions

Country Status (1)

Country Link
GB (1) GB887395A (en)

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