GB871541A - An improved ion-exchange reagent - Google Patents

An improved ion-exchange reagent

Info

Publication number
GB871541A
GB871541A GB16630/56A GB1663056A GB871541A GB 871541 A GB871541 A GB 871541A GB 16630/56 A GB16630/56 A GB 16630/56A GB 1663056 A GB1663056 A GB 1663056A GB 871541 A GB871541 A GB 871541A
Authority
GB
United Kingdom
Prior art keywords
divinyl benzene
ion
separation
insulin
styrene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB16630/56A
Inventor
Norman Keith Boardman
Stanley Miles Partridge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Research Development Corp UK
Original Assignee
National Research Development Corp UK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Development Corp UK filed Critical National Research Development Corp UK
Priority to GB16630/56A priority Critical patent/GB871541A/en
Publication of GB871541A publication Critical patent/GB871541A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/08—Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/16—Organic material
    • B01J39/17—Organic material containing also inorganic materials, e.g. inert material coated with an ion-exchange resin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J47/00—Ion-exchange processes in general; Apparatus therefor
    • B01J47/014—Ion-exchange processes in general; Apparatus therefor in which the adsorbent properties of the ion-exchanger are involved, e.g. recovery of proteins or other high-molecular compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Inorganic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)

Abstract

An ion-exchange reagent comprising a layer of styrene-divinyl benzene copolymer ion-exchange resin coated upon, and at least partially polymerized upon, the surface of an inert extender of large superficial area is used for the separation of protein and polypeptide molecules from their solutions. In examples styrene and divinyl benzene are polymerized in the presence of benzoyl peroxide, methyl alcohol or chloroform, and a diatomaceous earth treated with dichlorodimethyl silane, and the coated extender sulphonated to form the ion-exchange resin. Other conventional functional radicals may be used. The composite resin is used to separate the proteins in a solution of insulin, or the peptides from a mixture prepared by the hydrolysis of insulin with crystallized trypsin.ALSO:Proteins and polypeptides are separated from solutions containing them e.g. insulin, and a mixture of peptides prepared from the B-chain of insulin by hydrolysis with crystallised trypsin, by adsorbing upon an ion-exchange reagent comprising an ion-exchange resin based on a styrene-divinyl benzene copolymer coated and at least partially polymerised on an inert extender of large superficial area e.g. diatomaceous earth. The separation is by chromatographic methods and a copolymer of styrene-divinyl benzene containing 5 to 15% of divinyl benzene is preferred for the separation of protein and 0,5 to 2,1% of divinyl benzene for the separation of polypeptides of MW 500 to 5,000. Examples describe the use of sulphonated copolymer coated upon diatomaceous earth for the treatment of insulin solutions of varying purity, and the mixture of peptides referred to above.ALSO:Insulin is separated from solutions containing same by adsorbing upon an ion-exchange reagent comprising an ion-exchange resin based on a styrene-divinyl benzene copolymer coated, and at least polymerized upon an inert extender of large superficial area e.g. diatomaceous earth. The separation is by chromatographic methods and a copolymer of styrene-divinyl benzene containing 5 to 15.1 of divinyl benzene is preferred for the separation of proteins and 0.5 to 2.1 of divinyl benzene for the separation of polypeptides of MW 500 to 5000. Examples describe the use of sulphonated copolymer coated upon diatomaceous earth for the treatment of insulin solutions of varying purity and the mixture of peptides referred to above.
GB16630/56A 1956-05-29 1956-05-29 An improved ion-exchange reagent Expired GB871541A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB16630/56A GB871541A (en) 1956-05-29 1956-05-29 An improved ion-exchange reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB16630/56A GB871541A (en) 1956-05-29 1956-05-29 An improved ion-exchange reagent

Publications (1)

Publication Number Publication Date
GB871541A true GB871541A (en) 1961-06-28

Family

ID=10080794

Family Applications (1)

Application Number Title Priority Date Filing Date
GB16630/56A Expired GB871541A (en) 1956-05-29 1956-05-29 An improved ion-exchange reagent

Country Status (1)

Country Link
GB (1) GB871541A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3966596A (en) 1973-08-06 1976-06-29 The Dow Chemical Company High performance, cation-exchange chromatography on surface-sulfonated compositions
FR2439791A1 (en) * 1978-10-27 1980-05-23 Rhone Poulenc Ind Pure non-denatured plant protein prepn. - e.g. for human nutrition, comprises aq. extract contact with ion exchanger resin, silica or alumina (BE 28.4.80)
FR2470800A1 (en) * 1979-11-29 1981-06-12 Rhone Poulenc Ind PROCESS FOR PURIFYING BEET JUICE USING ION EXCHANGERS
US5081160A (en) * 1991-04-02 1992-01-14 The Dow Chemical Company Method of preparing uniform size ion exchange resin particles by partial functionalization
WO2013007799A1 (en) * 2011-07-13 2013-01-17 Instraction Gmbh Ion-exchanger material with high salt-tolerance

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3966596A (en) 1973-08-06 1976-06-29 The Dow Chemical Company High performance, cation-exchange chromatography on surface-sulfonated compositions
FR2439791A1 (en) * 1978-10-27 1980-05-23 Rhone Poulenc Ind Pure non-denatured plant protein prepn. - e.g. for human nutrition, comprises aq. extract contact with ion exchanger resin, silica or alumina (BE 28.4.80)
FR2470800A1 (en) * 1979-11-29 1981-06-12 Rhone Poulenc Ind PROCESS FOR PURIFYING BEET JUICE USING ION EXCHANGERS
US4331483A (en) 1979-11-29 1982-05-25 Rhone-Poulenc Industries Ion exchange purification of sugar beet juice
US5081160A (en) * 1991-04-02 1992-01-14 The Dow Chemical Company Method of preparing uniform size ion exchange resin particles by partial functionalization
WO2013007799A1 (en) * 2011-07-13 2013-01-17 Instraction Gmbh Ion-exchanger material with high salt-tolerance
JP2014524916A (en) * 2011-07-13 2014-09-25 インストラクション・ゲーエムベーハー High salt resistance ion exchange material

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