GB869034A - A medicinal polypeptide prepared from beef liver - Google Patents
A medicinal polypeptide prepared from beef liverInfo
- Publication number
- GB869034A GB869034A GB38665/57A GB3866557A GB869034A GB 869034 A GB869034 A GB 869034A GB 38665/57 A GB38665/57 A GB 38665/57A GB 3866557 A GB3866557 A GB 3866557A GB 869034 A GB869034 A GB 869034A
- Authority
- GB
- United Kingdom
- Prior art keywords
- liquid
- separating
- solid
- until
- residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/407—Liver; Hepatocytes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Virology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A polypeptide, enzymal in nature and useful in the treatment of arthritis (Group VI), has a molecular weight of 10,000 to 11,000 and approximately two glutamic acid residues per aspartic acid residue and also contains a minor proportion of arginine, lysine and glycine residues, it contains 60,5% glutamic acid, 30,5% aspartic acid, 3,3% arginine, 3,3% lysine and 2,4% glycine and is obtained from beef liver. The beef liver has the connective tissue removed manually and is then treated for the removal of proteins, albuminoids, histones, basic amino acids, proteoses and peptones, a water-miscible alcohol or ketone is added to the liquid residue in up to 60% concentration, the material allowed to stand at a low temperature for some hours until it forms a top liquid layer of an orange-red colour separated by a solid water of a grey or olive green colour from a lower liquid layer of a greenish-yellow colour, separating the upper liquid layer, adding thereto a water-miscible solvent which is an alcohol or ketone and cooling to a low temperature for some hours to form a flucculent, slightly pinkish precipitate, separating the solid from the liquid, macerating the solid with an aqueous liquid having a pH of 7 to 9, separating the liquid obtained and subjecting it to dialysis, and thereafter heating the dialysed liquid to 43 to 49 DEG C. until a heavy flocculent precipitate forms, separating the liquid, subjecting it to distillation at a reduced pressure and a temperature not exceeding 43 DEG C. until a solid residue is formed.ALSO:A polypeptide useful in the treatment of arthritis and having a molecular weight of 10,000 to 11,000 and approximately two glutamic acid residues per aspartic acid residue and also containing a minor proportion of arginine, lysine and glycine residues, contains 60.5% glutamic acid, 30.5% aspartic acid, 3.3% arginine, 3.3% lysine and 2.4% glycine and is isolated from beef liver. The beef liver has its connective tissue removed manually and is then treated for the removal of proteins, albuminoids, histones, basic amino acids, proteoses and peptones, a water-miscible alcohol or ketone is iadded to the liquid residue in a concentration not exceeding 60% by volume, the material is allowed to stand at a low temperature, e.g. 3 DEG C. for some hours, e.g. 12 hours until it forms a top liquid layer of an orange-red colour separated by a solid wafer of a grey or olive-green colour from a lower liquid layer of a greenish-yellow colour, separating the upper liquid layer, adding thereto a water-miscible alcohol or ketone, cooling to a low temperature, e.g. 3 DEG C. to form a flocculent slightly pinkish precipitate, separating the solid from the liquid, macerating the solid with an aqueous liquid having a pH of 7 to 9, separating the liquid obtained and subjecting it to dialysis, and thereafter heating the dialysed liquid to 43 DEG to 49 DEG C. until a heavy flocculent precipitate forms, separating the liquid, subjecting it to distillation at a reduced pressure and temperature not exceeding 43 DEG C. until a solid residue is formed.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB38665/57A GB869034A (en) | 1957-12-12 | 1957-12-12 | A medicinal polypeptide prepared from beef liver |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB38665/57A GB869034A (en) | 1957-12-12 | 1957-12-12 | A medicinal polypeptide prepared from beef liver |
Publications (1)
Publication Number | Publication Date |
---|---|
GB869034A true GB869034A (en) | 1961-05-25 |
Family
ID=10404914
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB38665/57A Expired GB869034A (en) | 1957-12-12 | 1957-12-12 | A medicinal polypeptide prepared from beef liver |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB869034A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2204409A1 (en) * | 1972-10-27 | 1974-05-24 | Palolab Pharmaceuti Als | Isolation of biologically active protein - with influence on primary carbohy-drate metabolism and the urea cycle and used to treat uraemia inflammation and shock |
-
1957
- 1957-12-12 GB GB38665/57A patent/GB869034A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2204409A1 (en) * | 1972-10-27 | 1974-05-24 | Palolab Pharmaceuti Als | Isolation of biologically active protein - with influence on primary carbohy-drate metabolism and the urea cycle and used to treat uraemia inflammation and shock |
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