GB841495A - A new antibiotic designated compound number 58 - Google Patents
A new antibiotic designated compound number 58Info
- Publication number
- GB841495A GB841495A GB1545/58A GB154558A GB841495A GB 841495 A GB841495 A GB 841495A GB 1545/58 A GB1545/58 A GB 1545/58A GB 154558 A GB154558 A GB 154558A GB 841495 A GB841495 A GB 841495A
- Authority
- GB
- United Kingdom
- Prior art keywords
- compound
- compound number
- sulphate
- sodium
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/28—Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
Abstract
<PICT:0841495/IV(b)/1> A new antibiotic designated Compound No. 58 is obtained by p cultivating a Compound No. 58-producing strain of Streptomyces fasciculus e.g. S. fasciculus ATCC 12703 (described) in a nutrient medium containing a source of carbon and nitrogen under submerged aerobic conditions. A temperature of 24 DEG to 35 DEG C. and a duration of cultivation of 1 to 6 days is suitable. Specified carbon sources are sugars, starch, glycerol and corn meal. Nitrogen sources are casein, soybean meal, peanut meal, wheat gluten, cottonseed meal, lactalbumin, tryptone and enzymatic digest of casein. Other additions are (a) growth substances such as distiller's solubles, yeast extract and molasses fermentation residues; (b) mineral salts such as sodium chloride, calcium carbonate, potassium phosphate, sodium nitrate and magnesium sulphate; and (c) trace metals. Compound No. 58 is isolated from the broth by filtration using a filter aid and extraction of the mycelium cake with an alkanol such as methanol, ethanol, butanol and pentanol. The extract is concentrated in vacuo and the residue treated with a non-solvent such as hexane and the solid Compound No. 58 filtered off. Compound No. 58 has fungicidal activity and in the crude or purified form is formulated into compositions for the treatment of plants (see Group VI). It is an amphoteric compound forming salts with acids such as sulphuric, hydrochloric, nitric, hydrobromic, benzene sulphonic, toluene sulphonic, trichloracetic and sulphurous, and with bases such as hydroxides and oxides of the alkali metals and alkaline earth metals and triethylamine sulphate; gives violet colour with conc.H2SO4; in 70% n-propanol water has R6 value of 0.70; is soluble in pyridine and N,N-di-methylformamide, insoluble in water, methyl isobutyl ketone, benzene, nitromethane, acetonitrile, ethyl acetate and methylcyclohexane; decomposes at 123 DEG C., melts at 250 DEG C.; [a ]5D = + 302 DEG (0.2% pyridine); contains carbon 59.94%, hydrogen 8.29%, nitrogen 1.96% and oxygen 29.81%; ultraviolet absorption in 80% aqueous methanol 0.001 N in NaOH e 1%1 cm = 1238 at 349 mm , = 1200 at 332 mm , = 753 at 317 mm and = 358 at 303 mm ; infrared absorption spectrum exhibits peaks at 3279, 2865, 1712, 1695, 1613, 1560, 1414, 1368, 1168, about 1099, 1066, 1006, 963, 918, 885, 842 and 789 reciprocal centimetres (Fig. II). In examples: (a) the sodium salt is prepared by adding methanolic sodium hydroxide to a solution of compound number 58 in methanol (b) compound number 58 is obtained from the sodium salt by adjusting a methanol solution of the sodium salt to pH 7.0 by means of phosphoric acid; (c) the triethylamine sulphate salt is prepared by adding triethylamine sulphate to a methanol solution of compound number 58 and adding acetone to precipitate the said salt; (d) the sulphate and hydrochloride are prepared by adding sulphuric acid or hydrochloric acid to an acetone solution of compound number 58 to pH 3.2 and precipitating by means of ethyl acetate.ALSO:A fungicidal composition comprises an inert carrier and at least three parts per million of the antibiotic, compound number 58, or a salt thereof such as the sulphate, hydrochloride, hydrobromide, hydroiodide, benzene sulphonate, toluene sulphonate, trichloracetate and the sodium, potassium, barium, lithium and calcium salts. Compound number 58 may be used in the form of the whole broth, as such or concentrated, the mycelium cake or as the purified product. Sprays, emulsions, injectionable solutions, suspensions and dusts are used containing 0,01 to 95% of the antibiotic. Suitable carriers are pyrophilite, bentonite, talc, kaolin, clay, waxes and resins including natural resins or gums. Stabilisers and spreading agents may also be present. A spray may be prepared by suspending the antibiotic in 5% aqueous acetone.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US841495XA | 1957-01-28 | 1957-01-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB841495A true GB841495A (en) | 1960-07-13 |
Family
ID=22183169
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1545/58A Expired GB841495A (en) | 1957-01-28 | 1958-01-16 | A new antibiotic designated compound number 58 |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB841495A (en) |
-
1958
- 1958-01-16 GB GB1545/58A patent/GB841495A/en not_active Expired
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