GB802537A - The recovery of metabolic products - Google Patents
The recovery of metabolic productsInfo
- Publication number
- GB802537A GB802537A GB11088/56A GB1108856A GB802537A GB 802537 A GB802537 A GB 802537A GB 11088/56 A GB11088/56 A GB 11088/56A GB 1108856 A GB1108856 A GB 1108856A GB 802537 A GB802537 A GB 802537A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cells
- vitamin
- recovery
- serum
- bacitracin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Mycology (AREA)
- Genetics & Genomics (AREA)
- Epidemiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Metabolic products are recovered from bacterial or mould cultures in a process which comprises adding a specific antiserum to a culture medium to precipitate or agglutinate the bacteria or mould cells, separating the precipitated or agglutinated cells from the culture medium and recovering the desired metabolic product from the separate cells or from the culture liquid. The process is suitable for the recovery of Vitamin B12 from cultures of Propionibacterium freudenreichii, Bacillus megatherium and strains of Streptomyces, the recovery of streptomycin from strains of Streptomyces, the recovery of bacitracin from B. licheniformis and B. subtilis spp. Tracy 1, and for the recovery of Vitamin B12 from Clostridium acetobutylicum in acetone/butanol fermentations. The antiserum may be obtained by injecting an animal with increasing concentrations of the bacterium over a series of days and recovering the antiserum from the animal. In an example Vitamin B12 is obtained by adding a specific antiserum for Propionibacterium freudenreichii to a fermentation broth containing cells of that organism, separating the bacterial cells and liquid by centrifugation, extracting the Vitamin B12 from the cells by treatment under acid conditions, purifying the Vitamin B12 by extraction into an organic solvent and re-extraction into an aqueous phase and recovering the purified Vitamin B12. The example also indicates that optimum agglutination occurs at serum/serum +broth dilutions in the range 1:10,000 to 1:15,000. The process may be employed in the production of bacitracin, the acidified liquid remaining after the cells of B. subtilis have been removed, being extracted with an organic solvent such as butanol, followed by the adsorption of impurities on charcoal, and recovery of the bacitracin by evaporation under reduced pressure.ALSO:An anti-serum for a specific bacterium is obtained by inoculating an animal, for example a rabbit, horse, cow or ox, with increasing concentrations of the bacterium over a period of days. When the antibody titre of the blood is sufficiently high, blood is withdrawn and the serum separated from the coagulated cells by conventional methods. This process is illustrated by the production of an anti-serum specific for Propionibacterium freudenreichii by inoculating a horse. The anti-sera so obtained are utilized in the precipitation of bacteria or mould cells in fermentation process for the production of metabolic products, e.g. Vitamin B12 and bacitracin.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB11088/56A GB802537A (en) | 1956-04-12 | 1956-04-12 | The recovery of metabolic products |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB11088/56A GB802537A (en) | 1956-04-12 | 1956-04-12 | The recovery of metabolic products |
Publications (1)
Publication Number | Publication Date |
---|---|
GB802537A true GB802537A (en) | 1958-10-08 |
Family
ID=9979817
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB11088/56A Expired GB802537A (en) | 1956-04-12 | 1956-04-12 | The recovery of metabolic products |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB802537A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105219666A (en) * | 2014-06-18 | 2016-01-06 | 清华大学 | For producing the fungal component system and way of butanols under micro-oxygen conditions |
-
1956
- 1956-04-12 GB GB11088/56A patent/GB802537A/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105219666A (en) * | 2014-06-18 | 2016-01-06 | 清华大学 | For producing the fungal component system and way of butanols under micro-oxygen conditions |
CN105219666B (en) * | 2014-06-18 | 2021-03-26 | 清华大学 | Symbiotic bacteria system and method for producing butanol under micro-aerobic conditions |
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