GB799644A - Method for the dried storage of micro-organisms - Google Patents
Method for the dried storage of micro-organismsInfo
- Publication number
- GB799644A GB799644A GB37493/55A GB3749355A GB799644A GB 799644 A GB799644 A GB 799644A GB 37493/55 A GB37493/55 A GB 37493/55A GB 3749355 A GB3749355 A GB 3749355A GB 799644 A GB799644 A GB 799644A
- Authority
- GB
- United Kingdom
- Prior art keywords
- molar concentration
- per cent
- vacuo
- storing
- drying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
In making preparations of dried microorganisms to withstand storage and retain viability, substances which inhibit or prevent damage by reaction between cellular protein and available carbonyl compounds are incorporated. These substances are exemplified by non-reducing sugars, polyols, inositol, free amino acids reacting in the dry state with carbonyl radicals, sulphur dioxide, hydroxylamine, phenylhydrazine, semicarbazide, pyridinium aceto-hydrazide chloride (Girard reagent P) and trimethylammonium aceto hydrazide chloride (Girard T reagent). The method may be applied to vaccines comprising suspensions of bacteria, rickettsiae, pleuropneumonia type organisms and viruses, bacteria of leguminous plant root nodules, bacteria used in cheese-making, yeasts, and mammalian spermatozoa. Examples are concerned with (i) drying a culture, containing a 0.1 molar concentration of mannitol, of a Streptococcus lactis used in cheese-making, and storing in vacuo at 25 DEG C. and r.h. 40 per cent; (ii) drying tap water suspensions, containing a 1 molar concentration of sucrose and containing a 0.1 molar concentration of lysine and a 1 molar concentration of sucrose, of Rhizobium leguminosarum, a clover seed inoculant, and storing in vacuo at 25 DEG C. and r.g. 0 per cent; (iii) drying tap water and phosphate buffer solution suspensions of Salmonella newport containing a 1.1 molar concentration of sucrose and containing a 0.01 molar concentration of hydroxylamine, semi-carbazide, Girard reagent P or T, and storing in air, and in vacuo at 25 DEG C. and r.h. 0 per cent and r.h. 43 per cent; (iv) drying a baker's strain of Saccharomyces cerevisiae culture containing a 0.1 molar concentration of lysine, and storing in vacuo at r.h. 0 per cent; (v) drying broths of the viruses staphylococcus bacteriophage 51 and Escherichia coli bacteriophage T5 having a 0.1 molar concentration of lysine, a 0.1 molar concentration of glutamic acid, glycine and lysine in equal parts, a 0.5 molar concentration of sorbitol, or inositol, and storing in vacuo at 25 DEG C. and r.h. 43 per cent and r.h. 0 per cent; (vi) drying a strain of the virus for Herpes simplex in a buffered egg allantoic fluid containing a 0.1 molar concentration of lysine, and storing in vacuo at 25 DEG C. and r.h. 43 per cent and r.h. 0 per cent; (vii) retention of activity in dried human influenza virus by addition of alanine.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU799644X | 1955-01-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB799644A true GB799644A (en) | 1958-08-13 |
Family
ID=3759306
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB37493/55A Expired GB799644A (en) | 1955-01-19 | 1955-12-30 | Method for the dried storage of micro-organisms |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB799644A (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001037656A2 (en) * | 1999-11-22 | 2001-05-31 | Universal Preservation Technologies, Inc. | Preservation of sensitive biological material |
EP1123710A1 (en) * | 1998-10-19 | 2001-08-16 | Changchun Institute of Biological Products Ministry of Public Health | Freeze-dried hepatitis a attenuated live vaccine and its stabilizer |
US6872357B1 (en) | 2000-11-22 | 2005-03-29 | Quadrant Drug Delivery Limited | Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying |
-
1955
- 1955-12-30 GB GB37493/55A patent/GB799644A/en not_active Expired
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1123710A1 (en) * | 1998-10-19 | 2001-08-16 | Changchun Institute of Biological Products Ministry of Public Health | Freeze-dried hepatitis a attenuated live vaccine and its stabilizer |
EP1123710B1 (en) * | 1998-10-19 | 2005-08-03 | Changchun Institute of Biological Products Ministry of Public Health | Freeze-dried hepatitis a attenuated live vaccine and its stabilizer |
WO2001037656A2 (en) * | 1999-11-22 | 2001-05-31 | Universal Preservation Technologies, Inc. | Preservation of sensitive biological material |
WO2001037656A3 (en) * | 1999-11-22 | 2002-01-10 | Universal Preservation Technologies Inc | Preservation of sensitive biological material |
US6872357B1 (en) | 2000-11-22 | 2005-03-29 | Quadrant Drug Delivery Limited | Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying |
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