GB787607A - Toxin-inactivating substance and preparation - Google Patents

Abstract

An antibiotic with an antibacterial spectrum closely related to that of penicillin is produced by the surface culture of Penicillium cyaneofulvum ATCC 11923 on a beef infusion broth containing 1 per cent glucose at 20-25 DEG C. for 7 days.ALSO:The invention relates to noxiversin which is a protein substance derived from the albumen fraction of a culture of Penicillium cyaneofulrum ATCC 11923 free of mycelium and globulin, effective in detoxifying toxins, effective as a toxoiding agent, non-dialysable, non-antibiotic, non-antigenic, non-toxic, its detoxifying and toxoiding activity not being interfered with by Clarase (penicillin inactivating substance), thermolabile, free of copper sulphate precipitated material, free of dialysable substances, its toxin inactivating activity being attributable to its capability to act directly upon the toxia molecule in contrast to a blocking action. P. cyanco fulrum is surface cultured on glucose beef infusion broth for 7 days at 20 DEG to 25 DEG C. Less suitable is a medium containing malt extract, neopeptone and glucose. The mycelium is filtered off, the pH of the culture filtrate adjusted to pH 7.0, ammonium sulphate added to 48 per cent concentration and the precipitated globulin removed by centrifugation. The clear liquid is saturated with ammonium sulphate and the active albumen fraction precipitates and is removed. This is suspended in saline and dialysed against saline for 48 hours at 5 DEG C. and negative pressure of - 6 mm. Hg. The dialysed albumen fraction may then be lyophilized and stored in ampoules at 4-10 DEG C. Alternatively, a ten-fold purification is obtained by adding 1 per cent copper sulphate to an aqueous solution of the above albumen fraction and removing the inactive precipitate which is toxic to animals. The supernatant fluid is dialysed against saline to remove excess copper sulphate. Noxiversin is thermolabile, being completely inactivated in solution after 30 minutes at 70 DEG C. It is inactivated by fresh normal serum. It is used in the preparation of toxoids and antitoxic sera for medicinal and veterinary use.