GB735739A - Improvements in or relating to the production of heparin - Google Patents
Improvements in or relating to the production of heparinInfo
- Publication number
- GB735739A GB735739A GB1170253A GB1170253A GB735739A GB 735739 A GB735739 A GB 735739A GB 1170253 A GB1170253 A GB 1170253A GB 1170253 A GB1170253 A GB 1170253A GB 735739 A GB735739 A GB 735739A
- Authority
- GB
- United Kingdom
- Prior art keywords
- heparin
- added
- precipitate
- dye
- formamide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A crude heparin extract is mixed with a basic dye of the thiazine group, excess dye is removed from the resulting precipitate, the precipitate is then dissolved in a solvent which does not substantially affect the heparin and an electrolyte and a precipitating agent for the heparin added and the precipitated heparin recovered. The crude heparin extract may be prepared by known methods from lung or liver tissues but it is preferred to use an alkaline extract fully saturated with ammonium sulphate. Suitable dyestuffs include thionine and Azure A. The dye-heparin product may be dissolved in formamide, dimethylformamide or N-caustic soda. Electrolyte (sodium chloride) may be added to form a saturated solution in the liquid present and the heparin precipitated by addition of methanol, ethanol or acetone. The formation of the heparin dye complex may be carried out at temperatures not above 25-30 DEG C. In an example minced lung tissue is extracted with 2N caustic soda and ammonium sulphate and filtered. To the filtrate an aqueous solution of Azure A is added, the precipitate filtered off and washed with distilled water and 0.05 N hydrochloric acid to remove excess dyestuff. The washed precipitate is dissolved in formamide, and after centrifuging saturated sodium chloride and methyl alcohol added. The precipitated heparin is purified by redissolving in formamide, adding saturated sodium chloride and precipitating with methyl alcohol.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1170253A GB735739A (en) | 1953-04-28 | 1953-04-28 | Improvements in or relating to the production of heparin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1170253A GB735739A (en) | 1953-04-28 | 1953-04-28 | Improvements in or relating to the production of heparin |
Publications (1)
Publication Number | Publication Date |
---|---|
GB735739A true GB735739A (en) | 1955-08-24 |
Family
ID=9991130
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1170253A Expired GB735739A (en) | 1953-04-28 | 1953-04-28 | Improvements in or relating to the production of heparin |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB735739A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1156938B (en) * | 1957-09-23 | 1963-11-07 | Upjohn Company Eine Ges Nach D | Method for obtaining heparin |
-
1953
- 1953-04-28 GB GB1170253A patent/GB735739A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1156938B (en) * | 1957-09-23 | 1963-11-07 | Upjohn Company Eine Ges Nach D | Method for obtaining heparin |
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