GB731104A - Improvement in preparation of blood fraction for use in rh testing procedure - Google Patents
Improvement in preparation of blood fraction for use in rh testing procedureInfo
- Publication number
- GB731104A GB731104A GB5617/53A GB561753A GB731104A GB 731104 A GB731104 A GB 731104A GB 5617/53 A GB5617/53 A GB 5617/53A GB 561753 A GB561753 A GB 561753A GB 731104 A GB731104 A GB 731104A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- globulins
- albumin
- precipitate
- alpha
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A serum fraction for use in Rh testing procedures is produced by passing bovine blood serum through a column containing both cation and anion exchange resins in a proportion such that a solution having a pH of 6 to 8, and preferably 7.0, results on passage through the column of an acidic, basic or neutral serum, whereby a precipitate of euglobulins is formed in the solution. The precipitate is removed and to the resultant solution is added an alkali metal salt of a fatty acid having from 4 to 10 carbon atoms, or an alkali metal salt of acetyltryptophane, in an amount sufficient to bring the molarity of the solution with respect thereto within the range of from 0.15 to 0.3. The pH is adjusted to 6.8 to 7.4 and the solution heated to 75 DEG C. for 25 to 35 minutes. After cooling to 25 DEG C., the pH is adjusted to 5.5 to 5.8 and the precipitated denatured globulins are removed. The alkali metal salt of the fatty acid or acetyltryptophane is removed by, e.g., dialysis or ion exchange resins and the resultant solution, consisting of albumin and alpha-globulins, is adjusted to pH 7.0 and concentrated or dried. Alternatively, after removing the precipitated denatured globulins, the solution may be adjusted to pH 3.0 to 5.5, and preferably 4.4 to 4.8, to precipitate the alpha-globulins, cooled to 2 DEG to -1 DEG C. and chilled methanol added to at least 38 per cent by volume to precipitate the albumin, the alpha-globulins and albumin then being separated from the solution. The test solution contains 26 to 30 per cent of the albumin and alpha-globulin proteins in normal saline and has a pH of 7.0 to 7.4. The cation-exchange resins are such as have active phenolic, carboxylic or sulphonic groups which are saturated with hydrogen ions, e.g. phenol methylene sulphonic acid resin, nuclear sulphonic acid resin and polymerized acrylic acid resin. The anion exchange resins have active amine groups such as a polymer resulting from nitration and subsequent reduction of a styrene divinylbenzene copolymer.ALSO:A serum fraction for use in Rh testing procedures is produced by passing bovine blood serum through a column containing both cation and anion exchange resins in a proportion such that a solution having a pH of 6 to 8, and preferably 7.0, results on passage through the column of an acidic, basic or neutral serum, whereby a precipitate of euglobulins is formed in the solution. The precipitate is removed and to the resultant solution is added an alkali metal salt of a fatty acid having from 4 to 10 carbon atoms, or an alkali metal salt of acetyltryptophane, in an amount sufficient to bring the molarity of the solution with respect thereto within the range of from 0.15 to 0.5. The pH is adjusted to 6.8 to 7.4 and the solution heated to 75 DEG C. for 25 to 35 minutes. After cooling to 25 DEG C., the pH is adjusted to 5.5 to 5.8 and the precipitated denatured globulins are removed. The alkali metal salt of the fatty acid or acetyltryptophane is removed by e.g. dialysis or ion exchange resins and the resultant solution, consisting of albumin and alpha-globulins, is adjusted to pH7.0 and concentrated or dried. Alternatively, after removing the precipitated denatured globulins, the solution may be adjusted to pH 3.0 to 5.5, and preferably 4.4 to 4.8 to precipitate the alpha-globulins, cooled to 2 DEG to -1 DEG C. and chilled methanol added to at least 38 per cent by volume to precipitate the albumin, the alpha globulins and albumin then being separated from the solution. The test solution contains 26 to 30 per cent of the albumin and alpha globulin proteins in normal saline and has a pH of 7.0 to 7.4. The cation exchange resin are such as have active phenolic, carboxylic or sulphonic groups which are saturated with hydrogen ions e.g. phenol methylene sulphonic acid resin, nuclear sulphonic acid resin and polymerised acrylic acid resin. The anion exchange resins have active amine groups such as a polymer resulting from nitration and subsequent reduction of a styrene divinylbenzene copolymer.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US731104XA | 1952-02-27 | 1952-02-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB731104A true GB731104A (en) | 1955-06-01 |
Family
ID=22111792
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB5617/53A Expired GB731104A (en) | 1952-02-27 | 1953-02-27 | Improvement in preparation of blood fraction for use in rh testing procedure |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB731104A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11604026B2 (en) | 2019-03-14 | 2023-03-14 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
US11634257B2 (en) | 2017-10-09 | 2023-04-25 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
-
1953
- 1953-02-27 GB GB5617/53A patent/GB731104A/en not_active Expired
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11634257B2 (en) | 2017-10-09 | 2023-04-25 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
US11604026B2 (en) | 2019-03-14 | 2023-03-14 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
US11609043B2 (en) | 2019-03-14 | 2023-03-21 | Terumo Bct Biotechnologies, Llc | Lyophilization container fill fixture, system and method of use |
US11609042B2 (en) | 2019-03-14 | 2023-03-21 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
US11740019B2 (en) | 2019-03-14 | 2023-08-29 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
US11747082B2 (en) | 2019-03-14 | 2023-09-05 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
US11815311B2 (en) | 2019-03-14 | 2023-11-14 | Terumo Bct Biotechnologies, Llc | Lyophilization container fill fixture, system and method of use |
US11994343B2 (en) | 2019-03-14 | 2024-05-28 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
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