GB721484A - Improvements in or relating to the degradation of dextran - Google Patents
Improvements in or relating to the degradation of dextranInfo
- Publication number
- GB721484A GB721484A GB9729/52A GB972952A GB721484A GB 721484 A GB721484 A GB 721484A GB 9729/52 A GB9729/52 A GB 9729/52A GB 972952 A GB972952 A GB 972952A GB 721484 A GB721484 A GB 721484A
- Authority
- GB
- United Kingdom
- Prior art keywords
- dextran
- enzyme
- sulphate
- aspergillus
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2454—Dextranase (3.2.1.11)
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/08—Dextran
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Materials Engineering (AREA)
- Polymers & Plastics (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
An aminoacid-stimulated dextran-acclimated enzyme which degrades dextran in an aqueous medium (see Group V), is obtained by cultivating an Aspergillus mould, e.g. a strain of Aspergillus Wentii in a nutrient solution containing dextran (see Group VI). The enzyme may be separated from the culture medium by filtration and precipitated from the filtrate by adding acetone or dioxane. Alternatively, the enzyme may be salted out by adding ammonium sulphate, sodium sulphate or sodium chloride to the filtrate. The precipitate may be dried to yield a powder.ALSO:In making dextran of low molecular weight from dextran of relatively high molecular weight, an aminoacid-stimulated, dextran-acclimated enzyme from an Aspergillus mould, e.g. a strain of Aspergillus Wentii (see Groups IV(b) and VI) is mixed with dextran of high molecular weight in an aqueous medium, the mixture is kept until the desired degree of degradation is attained and dextran of low molecular weight is removed, e.g. precipitated by adding to the degraded mixture an organic precipitant such as alcohol. The degraded mixture may first be separated into layers by adding acetone and a degraded dextran fraction may then be precipitated from each layer. The precipitate may be dissolved in water and reprecipitated. The precipitates may be dried in vacuo. The action of the enzyme may be stopped by bringing the pH of the mixture into the range of about 2.0 to 3.0 or 9.0 to 10.0, e.g. when the viscosity of the mixture relative to water is 2.0. The enzyme may be added to a medium containing about 7 per cent. of dextran.ALSO:An Aspergillus mould, e.g. a strain of Aspergillus Wentii is cultivated in an aqueous nutrient solution containing dextran, e.g., from 5.0 to 50 g dextran per litre, and a source of aminoacids so that a dextran-acclimated, aminoacid-stimulated enzyme (see Group IV(b)) is produced. This enzyme is used to degrade dextran (see Group V). The nutrient solution may contain the following substances in g. per litre; peptone 5.0, dextran 10.0, magnesium sulphate 0.1, sodium chloride 0.1, ferrous sulphate 0.1, sodium chloride 0.1, ferrous sulphate 0.01, manganous sulphate 0.01, potassium dihydrogen phosphate 0.1, sodium acetate 2.0, potassium acetate 0.5, with or without the following vitamins in m.g. per litre :-nicotinic acid 1.0, riboflavin 0.5, thiamine 0.5, calcium pantothenate 0.5, pyridoxine 0.4, folic acid 0.01, biotin 0.001. The peptone may be replaced by 5.0 g casein hydrolysate per litre. The vitamins may be replaced by 0.4 g. yeast extract per litre.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US721484XA | 1951-06-21 | 1951-06-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB721484A true GB721484A (en) | 1955-01-05 |
Family
ID=22105888
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB9729/52A Expired GB721484A (en) | 1951-06-21 | 1952-04-17 | Improvements in or relating to the degradation of dextran |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB721484A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0085642A1 (en) * | 1982-01-29 | 1983-08-10 | The Goodyear Tire & Rubber Company | Belting and method of making same |
-
1952
- 1952-04-17 GB GB9729/52A patent/GB721484A/en not_active Expired
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0085642A1 (en) * | 1982-01-29 | 1983-08-10 | The Goodyear Tire & Rubber Company | Belting and method of making same |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US3677898A (en) | Acid protease and method of preparing the same | |
GB1280396A (en) | Glucose isomerase and process for the production thereof | |
GB1385274A (en) | Process for the preparation of zeaxanthin | |
GB1121308A (en) | Cephalosporins | |
GB1210997A (en) | Process for the production of lipase | |
GB1204306A (en) | Process for producing l-glutamic acid by fermentation | |
US3764476A (en) | Process for producing microbial cells | |
GB721484A (en) | Improvements in or relating to the degradation of dextran | |
GB1474313A (en) | Process for preparing a protein concentrate from a microbial cell mass and the protein concentrate thus obtained | |
US2660551A (en) | Method for the production of dextran of relatively low molecular weight | |
US2673828A (en) | Preparation and use of polysaccharide-producing enzyme | |
GB1211246A (en) | Method for producing citric acid by fermentation | |
US3737383A (en) | Process for production of enzyme alkaline dextranase | |
US2716237A (en) | Production of endo-dextranase by aspergillus wenth | |
US2841578A (en) | Method for producing clinical dextran | |
Barker et al. | Nitrogen and vitamin requirements for axenic growth of the haustorial mycoparasite, Dispira cornuta | |
US2689204A (en) | Culture medium containing bismuthyl polyhydroxy polysulfite | |
US3654089A (en) | Production of amylo-1,6-glucosidase | |
US3627639A (en) | Process for producing l-asparaginase | |
US4033821A (en) | Process for producing yeast cells | |
US3658653A (en) | Process for the preparation of ergotamine and ergocryptine | |
GB1147229A (en) | Process for producing l-lysine from 5-(4-aminobutyl)-hydantoin | |
GB775948A (en) | Production of endo-dextranase by an aspergillus mold | |
US3028310A (en) | Method of producing l-valine by fermentation | |
US2123078A (en) | Fermentation mash |