GB687500A - An antibiotic and the preparation thereof - Google Patents

An antibiotic and the preparation thereof

Info

Publication number
GB687500A
GB687500A GB12048/50A GB1204850A GB687500A GB 687500 A GB687500 A GB 687500A GB 12048/50 A GB12048/50 A GB 12048/50A GB 1204850 A GB1204850 A GB 1204850A GB 687500 A GB687500 A GB 687500A
Authority
GB
United Kingdom
Prior art keywords
viomycin
sulphate
antibiotic
methanol
pontamine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB12048/50A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Inc
Original Assignee
Pfizer Inc
Charles Pfizer and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Inc, Charles Pfizer and Co Inc filed Critical Pfizer Inc
Publication of GB687500A publication Critical patent/GB687500A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D225/00—Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom
    • C07D225/04—Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom condensed with carbocyclic rings or ring systems
    • C07D225/06—Heterocyclic compounds containing rings of more than seven members having one nitrogen atom as the only ring hetero atom condensed with carbocyclic rings or ring systems condensed with one six-membered ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/188—Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

An antibiotic "Viomycin" (Registered Trade Mark), is prepared from the fermentation broth obtained by cultivating a Viomycin-producing strain of Streptomyces puniceus, or a mutant thereof, in an aqueous nutrient medium containing carbohydrate, preferably adjusted to pH7, with submerged aerobic conditions, a temperature of 24 DEG to 30 DEG C. and 2 to 7 days fermentation. The culture medium contains carbohydrate, e.g. sugars, starch and glycerol; organic nitrogen, e.g. soybean meal and wheat gluten; growth substances, e.g. distillers' solubles; sodium chloride and buffering agents, e.g. calcium carbonate. The antibiotic may be isolated by (a) precipitation with acid fast dyes or (b) adsorption on a cationexchange resin. For (a) the preferred dye is Eriochrome Violet, others which may be used are: Erio Fast Cyanine, Pontamine Fast Orange, Alizarin Red S, Synthracine Blue, Pontamine Steel Blue, Polar Yellow, Methyl Orange and Pontamine Green. The dye salt formed may be treated, e.g. triethylamine sulphate in the presence of a solvent, e.g. methanol or methanol-acetone and the Viomycin sulphate filtered off. Alternatively a solution of the dye salt may be treated with an inorganic salt, e.g. Bacl or bacl2, and the precipitated metal dye salt removed. The antibiotic may be obtained from the resin eluate by freeze drying, or by the addition of solvents such as methanol and butanol. Two antibiotics are present in the fermentation broth; the major component Viomycin is isolated by the above methods. The preparation of the reineckate, picrate, b -naphthelene sulphonate, sulphate and hydrochloride of Viomycin is described. Viomycin is a basic antibiotic containing C,H,O and N and forming salts with organic and inorganic acids. The sulphate and hydrochloride (mpt. 265-8 DEG C.) are soluble in water, sparingly soluble in methanol, acetone and b -methoxyethanol. Viomycin sulphate [a ]25D = -31.3 DEG (H2O 1 per cent), E1 per cent1 cm. at 267.5 mm = 285. The micro organism S. puniceus is described.
GB12048/50A 1949-08-25 1950-05-15 An antibiotic and the preparation thereof Expired GB687500A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US687500XA 1949-08-25 1949-08-25

Publications (1)

Publication Number Publication Date
GB687500A true GB687500A (en) 1953-02-18

Family

ID=22084478

Family Applications (1)

Application Number Title Priority Date Filing Date
GB12048/50A Expired GB687500A (en) 1949-08-25 1950-05-15 An antibiotic and the preparation thereof

Country Status (1)

Country Link
GB (1) GB687500A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2403348A1 (en) * 1977-09-19 1979-04-13 Upjohn Co NEW ANTIBIOTIC AND ITS PREPARATION PROCESS

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2403348A1 (en) * 1977-09-19 1979-04-13 Upjohn Co NEW ANTIBIOTIC AND ITS PREPARATION PROCESS

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