GB547518A - Improvements in or relating to compositions capable of inhibiting agglutination of hemolysis in blood - Google Patents

Abstract

547,518. Blood preparations. ABBEY, A. (Witebsky, E., and Klendshoj, N. C.). Nov. 19, 1940, No. 16680. [Class 81 (i)] Blood for transfusion of the type known as 0 is conditioned so as to render it suitable for administration to patients of other types by inhibiting the effects of the isoagglutinins A and B, which occur therein and cause agglutination respectively of the A factor and B factor, by reacting the blood with specific substances denoted as A and B specific substances which render these isoagglutinins substantially ineffective. A type blood also is conditioned by inhibiting the B isoagglutinins therein by reacting it with B specific substance. The A specific substance is obtainable from tissues and excretions of animals and humans of A type, and B specific substance from those belonging to B type, their presence being ascertained by adding A cells to a serum known to contain the A isoagglutinin when absence of agglutination indicates the presence of the A specific substance and mutatis mutandis in the case of B specific substance. The specific substances are obtained by precipitation from aqueous solution by alcohol in presence of an electrolyte e.g. sodium acetate, redissolution and precipitation and deproteinization of the clear solution e.g. by shaking with chloroform in presence of butyl or amyl alcohol. The clear supernatant liquid is dialysed to remove smaller molecules and the solution treated with acetone to precipitate the active substance. Examples describe the preparation of A specific substance from peptone and of B specific substance from gastric juice. Methods of standardization of the active substances and details of their utilization in the preparation of transfusion blood are described in the Specification.