GB2616560A - Apparatus for analysing a sample - Google Patents

Apparatus for analysing a sample Download PDF

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Publication number
GB2616560A
GB2616560A GB2308975.8A GB202308975A GB2616560A GB 2616560 A GB2616560 A GB 2616560A GB 202308975 A GB202308975 A GB 202308975A GB 2616560 A GB2616560 A GB 2616560A
Authority
GB
United Kingdom
Prior art keywords
well plate
fluorescence
low
sample
cover
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
GB2308975.8A
Inventor
Peterson Kurt
Grant Benjamin
Cole Lindsay
Gillispie Gregory
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Photophysics Ltd
Original Assignee
Applied Photophysics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Photophysics Ltd filed Critical Applied Photophysics Ltd
Publication of GB2616560A publication Critical patent/GB2616560A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/251—Colorimeters; Construction thereof
    • G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/168—Specific optical properties, e.g. reflective coatings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6482—Sample cells, cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6484—Optical fibres
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0332—Cuvette constructions with temperature control

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The present invention relates to an apparatus (1) for analysing a sample, the apparatus comprising:- • (a) a light source (2), and • (b) a detector (9), wherein the apparatus (1) is configured for directing light along an excitation path (Ex) from the light source (2) to a sampling point, and wherein the detector (9) is for detecting fluorescence emitted along an emission path (Em) from a sample placed at the sampling point to determine a characteristic of the sample, wherein the apparatus comprises (1) a multi-well plate (7) for holding a plurality of said samples, wherein the multi-well plate (7) comprises a material with a low UV fluorescence background.

Claims (31)

1. An apparatus for analysing a sample, the apparatus comprising:- (a) a light source, and (b) a detector, wherein the apparatus is configured for directing light along an excitation path from the light source to a sampling point, and wherein the detector is for detecting fluorescence emitted along an emission path from a sample placed at the sampling point to determine a characteristic of the sample, wherein the apparatus comprises a multi-well plate for holding a plurality of said samples, wherein the multi-well plate comprises a material with a low UV fluorescence background.
2. An apparatus according to claim 1, wherein the apparatus is configured for directing light along an excitation path from the light source and focussing said light to the sampling point.
3. An apparatus according to any preceding claim, wherein the position of the sampling point and/or the multi-well plate is adjustable so that the position of the sampling point can be moved to each of a plurality of wells of the multi-well plate.
4. An apparatus according to any preceding claim, wherein the multi-well plate comprises a polyolefin or a fluoropolymer with a low UV fluorescence background, preferably polypropylene, polyethylene or a fluoropolymer with a low UV fluorescence background, preferably polypropylene or a fluoropolymer with a low UV fluorescence background.
5. An apparatus according to any preceding claim, wherein the multi-well plate comprises ethylene tetrafluoroethylene (ETFE), perfluoroalkoxy alkanes (PFA), ethylene chlorotrifluoroethylene (ECTFE), polyvinylidene difluoride (PVDF), fluorinated ethylene propylene (FEP) or polytetrafluoroethylene (PTFE).
6. An apparatus according to any preceding claim, wherein the multi-well plate comprises a cover.
7. An apparatus according to claim 6, wherein the cover is for sealing one or more, preferably all, of the wells of the multi-well plate.
8. An apparatus according to claim 6 or 7, wherein the cover comprises a material with a low UV fluorescence background.
9. An apparatus according to any of claims 6 to 8, wherein the cover comprises a polyolefin or a fluoropolymer with a low UV fluorescence background, preferably polypropylene, polyethylene or a fluoropolymer with a low UV fluorescence background, preferably polypropylene or a fluoropolymer with a low UV fluorescence background.
10. An apparatus according to any of claims 6 to 9, wherein the cover comprises ethylene tetrafluoroethylene (ETFE), perfluoroalkoxy alkanes (PFA), ethylene chlorotrifluoroethylene (ECTFE), polyvinylidene difluoride (PVDF), fluorinated ethylene propylene (FEP) or polytetrafluoroethylene (PTFE).
11. An apparatus according to any of claims 6 to 10, wherein the cover comprises an adhesive for securing the cover to the multi-well plate, wherein the adhesive comprises a plurality of apertures for allowing (i) a beam of light travelling along the excitation path to pass therethrough and reach a sample held within a well of the multi-well plate, or (ii) a beam of fluorescence travelling from the sample along the emission path to pass therethrough.
12. An apparatus according to any of claims 6 to 11, wherein the cover is a film, preferably wherein the cover is a film with a thickness in the range of about 50 to about 200 pm, preferably in the range of about 50 to about 100 pm, preferably about in the range of about 50 to about 75pm.
13. An apparatus according to any preceding claim, wherein light is focussed to the sampling point from above the sampling point and wherein fluorescence travels along the emission path in a vertical direction away from the sampling point.
14. An apparatus according to any preceding claim, wherein the apparatus comprises an emission focussing lens for focussing fluorescence travelling along the emission path to a slit, aperture or fibre-optic input of the detector.
15. An apparatus according to any preceding claim, wherein the apparatus comprises a first emission path lens and a second emission path lens, wherein (i) the first emission path lens is for directing fluorescence from the sample along the emission path towards the second emission path lens, and (ii) the second emission path lens is for directing fluorescence to a slit, aperture or fibre-optic input of the detector.
16. An apparatus according to any preceding claim, wherein the apparatus comprises a temperature controlling device for controlling the temperature of the multi-well plate, preferably, the temperature controlling device is for controlling the temperature of the multi-well plate in the range of about 4 °C to about 120 °C, preferably in the range of about 20 °C to about 100 °C, preferably in the range of about 40 °C to about 90 °C, and/or wherein the temperature controlling device is a thermoelectric heat pump.
17. An apparatus according to any preceding claim, wherein the apparatus comprises a heated lid for the multi-well plate.
18. An apparatus according to claim 17, wherein the lid comprises a material with a low UV fluorescence background.
19. An apparatus according to claim 17 or 18, wherein the lid comprises a plurality of apertures for allowing (i) a beam of light travelling along the excitation path to pass therethrough and reach a sample held within a well of the multi-well plate, or (ii) a beam of fluorescence travelling from the sample along the emission path to pass therethrough.
20. An apparatus according to any of claims 17 to 19, wherein the multi-well plate comprises a cover and the lid is positioned over the cover.
21. An apparatus according to any preceding claim, wherein the detector comprises a spectrometer.
22. An apparatus for analysing a sample, the apparatus comprising:- (a) a light source, and (b) a detector, wherein the apparatus is configured for directing light along an excitation path from the light source and focussing said light to a sampling point, and wherein the detector is for detecting fluorescence emitted along an emission path from a sample placed at the sampling point to determine a characteristic of the sample, wherein the apparatus is configured for restricting the width of the excitation path as the light is focussed to the sampling point such that when the sampling point is within the well of a multi-well plate, the excitation path does not pass through a wall of the multi-well plate before it reaches the sample.
23. An apparatus according to claim 22, wherein the apparatus comprises a multi-well plate.
24. A method for analysing a plurality of samples, the method comprising:- (i) providing a plurality of samples wherein each sample is positioned in a well of a multi-well plate, (ii) analysing the samples using an apparatus which comprises (a) a light source, and (b) a detector, wherein the apparatus is configured for directing light along an excitation path from the light source to a sampling point, and wherein the detector is for detecting fluorescence emitted along an emission path from a sample placed at the sampling point to determine a characteristic of the sample, wherein the multi-well plate comprises a material with a low UV fluorescence background.
25. A method for analysing a plurality of samples, the method comprising:- (i) providing a plurality of samples in wells of a multi-well plate, and (ii) analysing the samples using an apparatus according to any of claims 1 to 23.
26. An apparatus according to claim 23 or a method according to claim 25, wherein the multi-well plate comprises a material with a low UV fluorescence background.
27. Use of a heated lid to reduce the UV fluorescence of a cover, wherein the cover is for a multi-well plate for holding a plurality of samples, wherein the multi-well plate comprises a material with a low UV fluorescence background.
28. Use according to claim 27, wherein the heated lid heats the cover to a temperature in the range of about 100 °C to about 120 °C.
29. Use according to claim 27 or claim 28, wherein the cover comprises a material with a low UV absorbance and a low UV fluorescence.
30. Use according to any of claims 27 to 29, wherein the cover comprises a polyolefin or a fluoropolymer with a low UV fluorescence background, preferably polypropylene, polyethylene or a fluoropolymer with a low UV fluorescence background, preferably polypropylene or a fluoropolymer with a low UV fluorescence.
31. Use according to any of claims , further comprising the feature of any of claims 4 to 11 or 17 to 20.
GB2308975.8A 2020-11-23 2021-11-22 Apparatus for analysing a sample Pending GB2616560A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB2018348.9A GB202018348D0 (en) 2020-11-23 2020-11-23 Apparatus for analying a sample
PCT/GB2021/053015 WO2022106847A1 (en) 2020-11-23 2021-11-22 Apparatus for analysing a sample

Publications (1)

Publication Number Publication Date
GB2616560A true GB2616560A (en) 2023-09-13

Family

ID=74046947

Family Applications (2)

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GBGB2018348.9A Ceased GB202018348D0 (en) 2020-11-23 2020-11-23 Apparatus for analying a sample
GB2308975.8A Pending GB2616560A (en) 2020-11-23 2021-11-22 Apparatus for analysing a sample

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB2018348.9A Ceased GB202018348D0 (en) 2020-11-23 2020-11-23 Apparatus for analying a sample

Country Status (2)

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GB (2) GB202018348D0 (en)
WO (1) WO2022106847A1 (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048262A1 (en) * 1997-04-23 1998-10-29 Packard Instrument Company, Inc. Measurement of fluorescence
WO2004024330A2 (en) * 2002-09-12 2004-03-25 Quanta Biotech Limited Thermocycler and sample holder
US20060134644A1 (en) * 2003-10-28 2006-06-22 Dakota Technologies, Inc. Apparatus and methods for detecting target analyte
US20090290151A1 (en) * 2008-03-03 2009-11-26 Ajjer Llc Analysis of arrays by laser induced breakdown spectroscopy
WO2016094460A1 (en) * 2014-12-10 2016-06-16 Corning Incorporated Reinforced microplate

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998048262A1 (en) * 1997-04-23 1998-10-29 Packard Instrument Company, Inc. Measurement of fluorescence
WO2004024330A2 (en) * 2002-09-12 2004-03-25 Quanta Biotech Limited Thermocycler and sample holder
US20060134644A1 (en) * 2003-10-28 2006-06-22 Dakota Technologies, Inc. Apparatus and methods for detecting target analyte
US20090290151A1 (en) * 2008-03-03 2009-11-26 Ajjer Llc Analysis of arrays by laser induced breakdown spectroscopy
WO2016094460A1 (en) * 2014-12-10 2016-06-16 Corning Incorporated Reinforced microplate

Also Published As

Publication number Publication date
WO2022106847A1 (en) 2022-05-27
GB202018348D0 (en) 2021-01-06

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