GB2613149A

GB2613149A - Sample plate assembly and kit

Info

Publication number: GB2613149A
Application number: GB2116868.7A
Authority: GB
Inventors: Mark Allison John; Gwen-Ham Ho Alex
Original assignee: Ascend Diagnostics Ltd
Current assignee: Ascend Diagnostics Ltd
Priority date: 2021-11-23
Filing date: 2021-11-23
Publication date: 2023-05-31
Also published as: GB202116868D0; WO2023094808A1

Abstract

A holder 10 for holding a sample plate 1A (e.g. matrix-assisted laser desorption/ionization, MALDI, mass spectrometry, MS) comprises a fulcrum (110, figure 2) defining a rotational axis of the sample plate and delimiting a first R1 and second R2 region. Releasable retaining members 120 retain the sample plate in a first rotational arrangement. Applying force F to the sample plate pivots it to a second rotational arrangement. The retaining member may be a magnet, resilient biasing member, cam, or a latch. A sample plate sleeve comprises a frame (230, figure 14) adapted to surround the edges of the sample plate (1, figure 13), and a fulcrum (110, figure 14) defining a rotational axis of the sample plate delimiting a first and second region of the frame. The sample plate is configurable in a first rotational configuration (figure 13) wherein the plate is recessed below the frame, and a second rotational configuration wherein the plate and the frame are spaced apart. The sample plate is biased in the first configuration and is moved to the second by applying a force to the plate. The sleeve may comprise male and female members (232-233, figure 14) suitable for stacking a plurality of sleeves.

Description

SAMPLE PLATE ASSEMBLY AND KIT

Field

The present invention relates to a sample plates for matrix-assisted laser desorption/ionization, MALDI, mass spectrometry, MS.

Background to the invention

Typically, biological, for example clinical, samples for matrix-assisted laser desorption/ionization, MALDI, mass spectrometry, MS, are deposited, manually and individually by an operator, into respective wells on sample plates (also known as target plates). Generally, the sample plates are uniquely identified, for example with a unique device identifier, UDI. Generally, the wells are arranged in regular arrays and a position of a specific well may be identified by row and column.

Usually, the operator is directed to deposit a particular sample into a particular well, identified by its position, on a particular sample plate, for example according to a sample list for the particular sample plate. The sample list may be provided according to the UDI of the particular sample plate, for example responsive to the operator scanning the UDI. Following deposition of the particular sample, the operator may be required to confirm that the particular sample was deposited into the particular sample well on the particular sample plate. In this way, traceability of the samples to particular wells on particular sample plates may be provided. During MALDI MS analysis, the samples deposited on the sample plates are analysed, individually (including specifically according to the particular sample) and automatically (for example, under computer control), and respective MS results thus obtained may be associated with the samples, via their particular well positions on particular sample plates. In this way, traceability of the MS results to the samples may be provided. In this way, diagnosis for and treatment of a given patient, according to corresponding MALDI MS sample analysis, may be provided.

However, the sample plates require handling before MALDI MS analysis, for example during deposition and/or during introduction into the MALDI MS, for example. Contamination and/or destruction, for example, of the samples may occur upon contact therewith during handling, for example by accidentally or deliberately touching the samples. In this way, the samples may be compromised due to errors, typically human errors, and/or malicious actions, thereby adversely affecting patient safety and/or outcome. In this context, an error is accidental while a malicious action is deliberate. Nevertheless, both errors and malicious actions may result in incorrect diagnosis for and/or treatment of a given patient.

Hence, there is a need to improve handling of samples.

Summary of the Invention

It is one aim of the present invention, amongst others, to provide a sample plate holder and a sample plate sleeve which at least partially obviates or mitigates at least some of the disadvantages of the prior art, whether identified herein or elsewhere. For instance, it is an aim of embodiments of the invention to provide a sample plate holder that facilitates handling of sample plates for MALDI MS analysis, thereby avoiding contamination and/or destruction, for example, of the samples deposited on the sample plates. For instance, it is an aim of embodiments of the invention to provide a sample plate sleeve that facilitates handling of sample plates during sample deposition and/or storage before MALDI MS analysis, thereby avoiding contamination and/or destruction, for example, of the samples.

A first aspect provides an assembly comprising a sample plate holder having a first face and a reverse second face, wherein the first face comprises a set of holders, including a first holder, and a respective set of sample plates, including a first sample plate, wherein the set of holders, including the first holder, is configured to hold the respective set of sample plates, including the first sample plate, therein and/or thereon and wherein the first sample plate has a planar first surface for depositing samples thereon and a mutually parallel, reverse second surface; wherein the first holder comprises: a fulcrum defining a rotational axis of the first sample plate and delimiting a first region of the first holder to a first side of the fulcrum and a second region of the first holder to a mutually opposed second side of the fulcrum; and a set of releasable retaining members, including a first releasable retaining member, disposed to the first side of the fulcrum; wherein the first sample plate is arrangeable in and/or on the first holder in: a first rotational arrangement about the fulcrum, wherein the first sample plate is releasably retained by the set of releasable retaining members and wherein the first surface defines a datum plane; and a second rotational arrangement about the fulcrum, wherein the first sample plate is released from the set of releasable retaining members and wherein the reverse second surface of the first sample plate and the first face of the sample plate holder are mutually spaced apart to the first side of the fulcrum; wherein the first sample plate is arranged to move from the first rotational arrangement to the second rotational arrangement by applying a force on the first surface of the first sample plate to the second side of the fulcrum.

A second aspect provides a sample plate holder having a first face and a reverse second face, wherein the first face comprises a set of holders, including a first holder, configured to hold a respective set of sample plates, including a first sample plate, therein and/or thereon, wherein the first sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the first holder comprises: a fulcrum defining a rotational axis of the first sample plate and delimiting a first region of the first holder to a first side of the fulcrum and a second region of the first holder to a mutually opposed second side of the fulcrum; and a set of releasable retaining members, including a first releasable retaining member, disposed to the first side of the fulcrum, for releasably retaining the first sample plate in a first rotational arrangement about the fulcrum, wherein the first surface of the first sample plate defines a datum plane.

A third aspect provides a kit comprising a sample plate sleeve and a sample plate, wherein the sample plate sleeve is configured to receive the sample plate therein and/or thereon, wherein the sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the sample plate sleeve comprises: a frame adapted to surround edges of the sample plate; and a fulcrum defining a rotational axis of the sample plate and delimiting a first region of the frame to a first side of the fulcrum and a second region of the frame to a mutually opposed second side of the fulcrum; wherein the sample plate is configurable in: a first rotational configuration about the fulcrum, wherein the first surface of the sample plate is recessed below the frame; and a second rotational configuration about the fulcrum, wherein the reverse second surface of the sample plate and the frame are mutually spaced apart to the first side of the fulcrum; wherein the sample plate is biased in the first rotational configuration and configured to move from the first configuration to the second configuration by applying a force on the first surface of the first sample plate to the second side of the fulcrum.

A fourth aspect provides a sample plate sleeve configured to receive a sample plate therein and/or thereon, wherein the sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the sample plate sleeve comprises: a frame adapted to surround edges of the sample plate; and a fulcrum defining a rotational axis of the sample plate and delimiting a first region of the frame to a first side of the fulcrum and a second region of the frame to a mutually opposed second side of the fulcrum.

Generally, as described herein: 1. The sample plate is a metal or metallized plate with controlled tolerance and flatness onto which the sample is deposited.

2. The sample plate holder is used to contain the sample plates and to provide a secure way of introducing the sample plates into the MS for analysis. The sample holder may play the role of an adaptor to accommodate the sample plate shown or alternative plates for different applications.

3. The sample sleeve is a handling aid for the sample plate during the spotting of the sample onto the plate and also acts as a protective frame for storage and transport.

Detailed Description of the Invention

According to the present invention there is provided an assembly comprising a sample plate holder and a sample plate, as set forth in the appended claims. Also provided is a sample plate holder; a kit comprising a sample plate sleeve and a sample plate; and a sample plate sleeve. Other features of the invention will be apparent from the dependent claims, and the description that follows.

Assembly comprising a sample plate holder and a sample plate The first aspect provides an assembly comprising a sample plate holder having a first face and a reverse second face, wherein the first face comprises a set of holders, including a first holder, and a respective set of sample plates, including a first sample plate, wherein the set of holders, including the first holder, is configured to hold the respective set of sample plates, including the first sample plate, therein and/or thereon and wherein the first sample plate has a planar first surface for depositing samples thereon and a mutually parallel, reverse second surface; wherein the first holder comprises: a fulcrum defining a rotational axis of the first sample plate and delimiting a first region of the first holder to a first side of the fulcrum and a second region of the first holder to a mutually opposed second side of the fulcrum; and a set of releasable retaining members, including a first releasable retaining member, disposed to the first side of the fulcrum; wherein the first sample plate is arrangeable in and/or on the first holder in: a first rotational arrangement about the fulcrum, wherein the first sample plate is releasably retained by the set of releasable retaining members and wherein the first surface defines a datum plane; and a second rotational arrangement about the fulcrum, wherein the first sample plate is released from the set of releasable retaining members and wherein the reverse second surface of the first sample plate and the first face of the sample plate holder are mutually spaced apart to the first side of the fulcrum; wherein the first sample plate is arranged to move from the first rotational arrangement to the second rotational arrangement by applying a force on the first surface of the first sample plate to the second side of the fulcrum.

In this way, the first sample plate is releasably retained by the set of releasable retaining members in the first rotational arrangement, wherein the first surface thereof defines the datum plane for MALDI MS of the samples deposited thereon. For example, the datum plane may define the length of a flight path of a time of flight (TOF) MALDI MS and hence the datum plane is typically accurately and/or precisely positioned for accurate and/or precise MS of the sample deposited thereon. Since the first sample plate is arranged to move from the first rotational arrangement to the second rotational arrangement by applying the force on the first surface of the first sample plate to the second side of the fulcrum, for example by an operator pressing the first surface using a finger, handling of the first sample plate is facilitated, thereby avoiding contamination and/or destruction, for example, of the samples deposited on the first sample plate. The first sample plate may be then removed more readily by holding edges thereof that have rotated away from the first face of the sample plate holder. Conversely, in one example, the first sample plate is arranged to move from the second rotational arrangement to the first rotational arrangement by releasing a force on the first surface of the first sample plate to the second side of the fulcrum, for example by an operator removing a finger from the first surface. For example, the first sample plate may be inserted in the second rotational arrangement and subsequently, lowered into the first rotational arrangement by removing a finger from the first surface. Particularly, the inventors have identified that inserting and removing sample plates via pivoting thereof about a fulcrum facilitates handling, thereby avoiding contamination and/or destruction, for example, of the samples deposited on the first sample plate. In contrast, conventional sample plate holders typically require tools, such as tweezers, for removing sample plates or include finger holes for an operator to hook a finger under the sample plates, resulting in accidental contact with the samples and/or damage of the sample plates. Hence, the assembly according to the first aspect provides tool-free insertion and removal of sample plates while not requiring an operator to hook a finger under the sample plates.

The sample plate is transported with the sample sleeve, as described below, to protect it from bending. It is then used as a handling frame for spotting the sample. Once samples are spotted and ready to be analysed in the MS, the sample plate is removed from the sample sleeve with a twist and pull action and placed onto the sample plate holder and may be held in place by magnets. The sample plate holder and sample plate are inserted into the MS.

Problems trying to solve: * Handling of thin sample 0.3 mm sample plates on a lab bench is difficult without handling aid. Risk of touching samples or bending plate when handled incorrectly.

* This is also true when removing the sample plate from the sample holder.

* A flat electrically conductive boundary/margin around the sample plate (without step) is preferably maintained so as not to generate disturbances of the electric field in the extraction region.

* The assembly is placed inside a vacuum chamber so consideration to simplicity and minimal parts to improve its loading time performance.

* Build-up of tolerance errors to be avoided to achieve better accuracy of the system by design.

Benefit of the System * No mechanical mechanism is involved in the removal of the sample plate, the action of pushing onto the non-sample region of the sample plate gives access to hold onto the edges of the sample plate for removal without touching the samples or stressing the plate.

* Between the MS and the sample, there are only two contacts, the MS-to-sample holder, and the sample plate holder-to-sample plate. This minimises the tolerance error build up in the extraction region of the mass spectrometer.

* The sample plate sleeve is designed to be low cost, it being a consumable part, cleaning for any contamination is not required (compared to re-useable sample handing tool).

* Sample Plate may be a ferrific grade of stainless, so magnets may be used to make the contact, mechanical locks are not required, makes it simple to place and remove the sample plate.

Sample plates The assembly comprises a set of sample plates, including the first sample plate (i.e. at least one sample plate). In one example, the set of sample plates includes S sample plates, wherein S is a natural number greater than or equal to 1, for example 1, 2, 3, 4, 5, 6 or more, preferably 2, 3 or 4. Each sample plate of the set thereof may be as described with respect to the first sample plate. Sample plates, for example for MALDI MS, are known. In one example, the first sample plate is a MALDI MS sample plate.

The first sample plate has the planar first surface for depositing samples thereon and the mutually parallel, reverse second surface.

Generally, a sample plate (also known as a target plate) is a generally rectangular or square flat plate, for example having mutually parallel upper and lower surfaces (i.e. the planar first surface and the mutually parallel, reverse second surface, respectively), formed from stainless steel or a polymeric composition comprising a polymer having an electrically conductive coating thereon for example, having an array, typically a regular array, of wells (i.e. concavities) in a first portion of an upper surface thereof, relatively more proximal a first end of the sample plate, for deposition of respective samples therein. A position of a specific well may be identified by row and column, which are typically labelled alphanumerically. Typically, a second portion of the upper surface, relatively more proximal a second end of the sample plate, mutually opposed to the first end, has no wells therein, for holding of the sample plate by an operator. To limit degrees of freedom when inserting a sample plate into a mass spectrometer, for example, the sample plate may have an asymmetric shape. A unique device identifier (UDI) may included in the second portion of the upper surface. Additionally and/or alternatively, the UDI may be included on the lower surface.

In one example, the first sample plate is a generally rectangular or square flat plate, having the planar first surface for depositing samples thereon and the mutually parallel, reverse second surface, formed from stainless steel or a polymeric composition comprising a polymer having an electrically conductive coating thereon for example, having an array, for example a regular array, of wells in a first portion of the first surface thereof, relatively more proximal a first end of the first sample plate, for depositing respective samples therein. In one example, the first sample plate includes human-readable, for example alphanumeric, labels to identify positions of the wells by row and column. In one example, a second portion of the first surface, relatively more proximal a second end of the first sample plate, mutually opposed to the first end, has no wells therein, for holding of the first sample plate by an operator. In one example, the first sample plate has an asymmetric shape, having a reduced number of planes of symmetry, for example a single plane of symmetry, or no planes of symmetry, orthogonal to the first surface. In one example, a first UDI is included, for example by printing, as a label, or engraving, in the second portion of the first surface of the first sample plate. Additionally and/or alternatively, in one example, the first UDI is included on the second surface of the first sample plate. In one example, the first sample plate is a single-use sample plate, not intended for reuse. In one example, the first sample plate is a multi-use sample plate, intended for reuse, for example following appropriate cleaning. In one example, a thickness, a flatness and/or a parallelism of the first sample plate is within 0.05 mm, preferably within 0.04 mm, more preferably within 0.03 mm. In this way, a height of the first sample plate, for example of the first surface thereof, may be arranged during MS analysis, for example for focusing a laser thereon and/or controlling a path length, or a part thereof, of the MS.

The Food and Drug Administration (FDA) established an unique device identification system, UDIS, to identify medical devices, sold in the United States, from manufacturing through distribution to patient use. Under the UDIS, medical device labels include a unique device identifier (UDI) in human-and machine-readable form. Medical device information corresponding to the UDI is submitted to a Global Unique Device Identification Database (GUDID). In this way, patient safety may be improved, device postmarket surveillance modernized, and medical device innovation facilitated through traceability conferred by the UDIs. A UDI is an unique numeric or alphanumeric code that typically includes: 1. Device identifier (DI): a mandatory, fixed portion of the UDI that identifies the labeller and the specific version or model of a device; 2. Production identifier (PI): a conditional, variable portion of the UDI that identifies one or more of the following when included on the label of the device: Lot or batch number within which the device was manufactured; Serial number of the specific device; Hi. Expiration date of the specific device; iv. Date the specific device was manufactured; v. Distinct identification code required by §1271.290(c) for a human cell, tissue, or cellular and tissue-based product (HCT/P) regulated as a device. The device labeller must provide the UDI in two forms on labels and packages: 1. Easily readable plain-text 2. Machine-readable form that uses automatic identification and data capture (AIDC) technology.

Automatic identification and data capture, AIDC, is any technology that conveys the UDI or the device identifier of a device in a form that may be entered into an electronic patient record or other computer system via an automated process. In addition, the device labeller must include dates on device labels and packages in a standard format that is consistent with international standards and international practice (YYYY-MM-DD). The European Commission through European Medicines Agency (EMA) and the International Medical Device Regulator Forum (IMDRF) have established similar requirements as the FDA. Sample plates, classified as medical devices, require respective UDIs to include a company number, an item reference number and a batch/lot number. In addition, once a sample has been deposited on a sample plate, traceability of the sample plate is required and so an unique serial number for the sample plate is also required.

In one example, the first UDI comprises and/or is a barcode, for example a 1D barcode or a 2D barcode such as a QR code or a Data Matrix, preferably a 2D barcode.

A barcode represents data in a visual, machine-readable form. One-dimensional (1D) or linear barcodes represent data by varying widths and spacings of parallel lines or bards and may be scanned using optical scanners, known as barcode readers. Two-dimensional (2D) barcodes use rectangles, dots, hexagons and other geometric patterns and may be known as matrix codes. Barcodes may be read using a reader including an imager such as a camera and decoded using software, for example image recognition and barcode decoding software. In this context, 2D barcodes are preferred due to their increased data storage capacity compared with 1D barcodes.

Numerous 1D barcode standards are known, including conforming with international ISO/IEC standards, for example. 1D barcode standards include: China Postal Code, UK Plessey Code, Industrial 2 of 5, Matrix 2 of 5m Interleaved 2 of 5, Code 128, TELEPEN, EAN/UCG/GS1-128, CODABAR, ABC-CODABAR, UPC-A, UPC-E, EAN-8, EAN-13, MSI, IATA, CODE 39, CODE 11, CODE 93, GS1 Databar, GS1 Databar Stacked, GS1 Databar Limited, GS1 Databar Expanded and GS1 Databar Expanded Stacked.

Numerous 2D barcodes are known, including conforming with international ISO/IEC standards, for example. 2D barcode standards include: PDF417, Data Matrix, OR code, Micro OR code, AZTEC, Code 1, DotCode and Snowflake code. PDF417, Data Matrix, OR code, AZTEC, Code 1, DotCode and Snowflake code may be used for pharmaceutical labels, amongst other applications A OR code is a two-dimensional code or symbol comprising a plurality of cells, each of said plurality of cells representing a binary-coded datum; said plurality of cells forming a two-dimensional matrix pattern readable by a scanning operation along any arbitrary scanning lines; and at least three positioning symbols disposed at predetermined positions in said two-dimensional matrix pattern, at least two of said at least three positioning symbols having a pattern capable of gaining an identical frequency component ratio irrespective of an orientation of said any arbitrary scanning lines whenever said arbitrary scanning lines pass through a center of each of said at least three positioning symbols. The amount of data that can be stored in a OR code depends on the datatype (mode, or input character set), version (1, ..., 40, indicating the overall dimensions of the symbol, i.e. 4 x version number + 17 dots on each side), and error correction level. The maximum storage capacity for version 40 and error correction level L (low), denoted by 40-L, is 2956 bytes.

A Data Matrix code is a two-dimensional code or symbol, comprising black and white cells arranged in either a square or a rectangular pattern, also known as a matrix. Depending on the coding used, a white cell represents 0 and a black cell represents 1, or vice versa. Every Data Matrix code includes two solid adjacent borders in an L shape (known as the finder pattern) and other two adjacent borders comprise alternating black and white cells (known as the timing pattern". Within these borders are rows and columns of cells encoding the information. Information encoded by the Data Matrix code may include text and/or numeric data. Code sizes vary from 10x10 to 144x144 in the new version ECC 200, and from 9x9 to 49x49 in the old version ECC 000 -140. The amount of data that can be stored in a Data Matrix code may be from a few bytes up to 1,556 bytes -the Data Matrix code can store up to 2,335 alphanumeric characters. Error correction codes are often used to increase reliability.

Sample plate holder The assembly comprises the sample plate holder having the first face and the reverse second face, wherein the first face comprises the set of holders, including the first holder (i.e. at least one holder), configured to hold (i.e. retain, secure) the respective set of sample plates, including the first sample plate, therein and/or thereon. In one example, the set of sample plate holders includes H holders, wherein H is a natural number greater than or equal to 1, for example 1, 2, 3, 4, 5, 6 or more, preferably 2,3 or 4. Conventional sample plate holders, for example for MALDI MS, are known. In one example, the first sample plate holder is a MALDI MS sample plate holder.

Generally, a sample plate holder (also known as a target plate holder) is a generally rectangular or square flat plate, for example having mutually parallel upper and lower surfaces (i.e. the first face and the reverse second face, respectively), formed from stainless steel or a polymeric composition comprising a polymer having a conductive coating thereon. A position of a specific holder may be identified by row and/or column, which are typically labelled alphanumerically. To limit degrees of freedom when inserting a sample plate into the sample plate holder, for example, the sample plate may have an asymmetric shape and the corresponding holder shaped accordingly. To limit degrees of freedom when inserting a sample plate holder into a MS, for example, the sample plate holder may have an asymmetric shape. A unique device identifier (UDI) may be included in and/or on the first face. Additionally and/or alternatively, the UDI may be included on the second face.

In one example, the sample plate holder is a generally rectangular or square flat plate, wherein the first face comprises and/or is a planar face and wherein the reverse second surface is mutually parallel thereto, formed from stainless steel or a polymeric composition comprising a polymer having a conductive coating thereon, wherein the first face comprises the set of holders, including the first holder. In one example, the first face comprises and/or is a planar face, for example defined by a single plane or by a set of mutually parallel planes, such as concavities therein. In one example, the reverse second face comprises and/or is a planar face, for example defined by a single plane. In one example, the sample plate holder includes human-readable, for example alphanumeric, labels to identify positions of the holders by row and/or column.

In one example, the sample plate holder has an asymmetric shape, having a reduced number of planes of symmetry, for example a single plane of symmetry, or no planes of symmetry, orthogonal to the first face. In one example, a first UDI is included, for example by printing, as a label, or engraving, on the first face. Additionally and/or alternatively, in one example, the first UDI is included on the second face. In one example, the sample plate holder is a multi-use sample plate holder, intended for reuse, for example following appropriate cleaning. In one example, a thickness, a flatness and/or a parallelism of the first face and/or the second face is within 0.05 mm, preferably within 0.04 mm, more preferably within 0.03 mm. In this way, a height of the sample plate holder, for example of the first face thereof, may be arranged during MS analysis, for example for focusing a laser thereon and/or controlling a path length, or a part thereof, of the MS.

The first face comprises the set of holders, including the first holder, configured to hold the respective set of sample plates, including the first sample plate, therein and/or thereon. In one example, the set of holders is provided in the sample plate holder such as in the first face, for example as a respective set of cavities in sample plate holder, for example machined or formed therein, optionally wherein respective perimeters (i.e. respective side walls) of the cavities have shapes corresponding with respective shapes of the sample plates, thereby constraining or preventing lateral movement thereof. In one example, the set of holders is provided on the first face, for example wherein respective perimeters thereof are defined by walls or pins upstanding therefrom, optionally wherein the respective perimeters have shapes corresponding with respective shapes of the sample plates, thereby constraining or preventing lateral movement thereof. That is, the set of holders may be provided in or on the first face. In one example, an uppermost surface of the assembly is planar (i.e. defined by a single plane) or substantially planar, thereby reducing or eliminating distortion of electric fields during MS. Each holder of the set thereof may be as described with respect to the first holder.

The first holder comprises the fulcrum defining the rotational axis of the first sample plate. In this way, the first sample plate is pivotable about the fulcrum, for example wherein the second surface of the first sample plate contacts the fulcrum and/or wherein opposite points at edges the first sample plate contact the fulcrum. In one example, the fulcrum is provided by an edge in the first holder, for example an edge of a concavity or a step in the first holder. In one example, the fulcrum is provided by pivot points at opposite sides of the holder.

The first holder comprises the fulcrum delimiting the first region (or area) of the first holder to the first side of the fulcrum and the second region (or area) of the first holder to the mutually opposed second side of the fulcrum. In one example, the first region corresponds or substantially corresponds with a first portion of the first surface of the first sample plate having an array, for example a regular array, of wells in the first portion of the first surface thereof, relatively more proximal a first end of the first sample plate, for depositing respective samples therein. In one example, the second region corresponds or substantially corresponds with a second portion of the first surface of the first sample plate, relatively more proximal a second end of the first sample plate, mutually opposed to the first end, having no wells therein, for holding of the first sample plate by an operator.

In one example, the first region of the first holder has a first depth relative to the first face of the sample plate holder, wherein the second region has a second depth relative to the first face of the sample plate holder and wherein the second depth is greater than the first depth. In this way, the first holder is provided as a cavity in the sample plate holder, as described previously, having a relatively lesser depth in the first region and a relatively greater depth in the second region. In this way, the first sample plate may rotate about the fulcrum, such that the first surface of the first sample plate is pressed into the second region at one end thereof while the second surface of the first sample plate is lifted above the first face in the first region at the other end thereof.

The first sample plate may be then removed more readily by holding edges thereof that have rotated away from the first face of the sample plate holder.

In one example, a ratio of the first depth to the second depth is in a range from 9: 10 to 1: 5, preferably in a range from 2: 3 to 1: 4, more preferably in a range from 1: 2 to 1 3.

In one example, the first depth corresponds with a thickness of the first sample plate. In this way, an uppermost surface of the assembly is planar (i.e. defined by a single plane) or substantially planar, thereby reducing or eliminating distortion of electric fields during MS.

In one example, the first region comprises and/or is a planar region, for example defining a reference plane for the second surface of the first sample plate in the first rotational arrangement. In this way, the datum plane is defined with reference to the reference plane and a thickness of the first sample plate. In one example, the planar region is provided by an array of pads, for example spaced apart by recesses and/or surrounded by relief channels. In this way, an accuracy and/or a precision of the region is improved since provision of the pads is facilitated compared with a monolithic planar region.

In one example, the first region has a first length orthogonal to the fulcrum, wherein the second region has a second length orthogonal to the fulcrum and wherein the first length is greater than the second length. In this way, the fulcrum is disposed relatively more proximal a second end of the first holder (i.e. the end of the second region) relative to a first end (i.e. the end of the first region) thereof. In this way, for a given angular rotation, a magnitude of linear displacement of the first end is relatively greater than a magnitude of linear displacement of the second end. In one example, the magnitude of linear displacement of the first end is in a range from 3 mm to 30 mm, preferably in a range from 5 mm to 25 mm, more preferably in a range from 10 mm to 15 mm. In this way, an operator may readily insert or remove the first sample plate in the second rotational arrangement, by holding edges thereof.

In one example, a ratio of the first length to the second length is in a range from 2: 1 to 25: 1, preferably in a range from 3: 1 to 15: 1, more preferably in a range from 5: 1 to 10: 1. In this way, an operator may readily insert or remove the first sample plate in the second rotational arrangement, by holding edges thereof.

The first holder comprises the set of releasable retaining members, including the first releasable retaining member (i.e. at least one releasable retaining member), disposed to the first side of the fulcrum. In one example, the set of releasable retaining members, including the first releasable retaining member, is disposed only to the first side of the fulcrum. In one example, the first holder does not comprise a releasable retaining member disposed to the second side of the fulcrum.

In one example, the first releasable retaining member comprises and/or is a magnet (such as provided in the first region, for example under the first sample plate) and/or a mechanical coupling member such as a resilient biasing member (such as a spring provided at an edge of the first holder arranged to press against an edge of the first sample plate), a cam (such as an interference provided between an edge of the first holder and the first sample plate) and/or a latch (such as a pivoted finger provided at an edge of the first holder and extending over the first surface of the first sample plate). Magnets are suitable for ferromagnetic samples plates (e.g. ferritic or duplex stainless steel) and avoid use of mechanical coupling members, thereby reducing complexity and/or moving parts. In this way, the first sample plate is releasably retained in the first rotational arrangement.

In one example, the set of releasable retaining members includes a second releasable retaining member. That is, the set of releasable retaining members may include a plurality of releasable retaining members. In one example, the set of releasable retaining members includes R releasable retaining members, wherein R is a natural number greater than or equal to 1, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more, preferably 2, 4 or 6. Each releasable retaining members of the set thereof may be as described with respect to the first releasable retaining member.

In one example, the first releasable retaining member is disposed at a first distance orthogonal to the fulcrum, wherein the second releasable retaining member is disposed at a second distance orthogonal to the fulcrum, wherein the second distance is greater than the first distance and wherein a first force required to release the first releasable retaining member is greater than a second force required to release the second releasable retaining member. In this way, a torque required to release the respective releasable retaining members of the set thereof may be substantially similar. In contrast, if the second force required to release the second releasable retaining member were the same as, or substantially similar to, the first force required to release the first releasable retaining member, a second torque required to release the second releasable retaining member would be greater than a first torque required to release the first releasable retaining member. In one example, a torque required to release the respective releasable retaining members of the set thereof is substantially similar, for example with 50% or within 25% of a mean torque thereof.

Rotational arrangements The first sample plate is arrangeable in and/or on the first holder in the first rotational arrangement about the fulcrum, wherein the first sample plate is releasably retained by the set of releasable retaining members and wherein the first surface defines the datum plane. It should be understood that the first rotational arrangement is for MS analysis of samples deposited on the first sample plate.

The first sample plate is arrangeable in and/or on the first holder in the second rotational arrangement about the fulcrum, wherein the first sample plate is released from the set of releasable retaining members and wherein the reverse second surface of the first sample plate and the first face of the sample plate holder are mutually spaced apart to the first side of the fulcrum. It should be understood that the second rotational arrangement is for insertion or removal of the first sample plate to or from the first holder, respectively.

The first sample plate is arranged to move from the first rotational arrangement to the second rotational arrangement by applying the force on the first surface of the first sample plate to the second side of the fulcrum, as described previously.

In one example, the first sample plate is rotatable by an angle in a range from 3° to 300, preferably in a range from 5° to 25°, more preferably in a range from 7° to 15°, for example 100 about the fulcrum.

In one example, the first holder is arranged to receive the first sample plate therein and/or thereon in the second rotational arrangement and by subsequently moving the first sample plate from the first rotational arrangement to the second rotational arrangement. In this way, the first sample plate is inserted into the holder in the second rotational arrangement, for example by an operator, and subsequently moved from the first rotational arrangement to the second rotational arrangement, for example by releasing the first sample plate whereby the first sample plate rotates about the fulcrum into the second rotational arrangement. Removal of the first sample plate is reversed.

In one example, the first holder is adapted to limit sliding, for example parallel to the datum plane, of the first sample plate in the first arrangement. In this way, a lateral (e.g. x and/or y) position of the first sample plate is defined by the first holder in the first rotational arrangement.

Conventionally, a single sample plate holder is integrated into a MS such that the sample plate holder is not removable therefrom by the operator. In contrast, the sample plate holder may be loaded into the MS and unloaded therefrom, for example repeatedly. In this way, samples may be deposited on sample plates that are then held in and/or on the sample plate holder, for example remote from the MS such as in another laboratory, by the operator and/or a different operator and the sample plate holder subsequently loaded into the MS, for mass spectrometry of samples included thereon. Additionally and/or alternatively, while the sample plate holder is loaded in the MS, other sample plates may be prepared in parallel (i.e. simultaneously) and held in and/or on a second sample plate holder, for subsequent loading into the MS. Additionally and/or alternatively, a plurality of sample plate holders may be stored an autosampler included in the MS. In this way, throughput of samples may be increased.

In one example, the sample plate holder is a generally rectangular or square flat plate, for example having mutually parallel upper and lower surfaces, formed from stainless steel or a polymeric composition comprising a polymer having a conductive coating thereon for example, having an array, for example a regular array, of female members (also known as placeholders) in and/or on a first portion of an upper surface thereof, relatively more proximal a first end of the sample plate holder, for receiving respective sample plates therein. In one example, each female member has a shape corresponding with a shape, for example an asymmetric shape, of a respective sample plate. In one example, each female member comprises a plurality of sidewall portions arranged to retain a respective sample plate in one or two dimensions. in one example, the sample plate holder, for example each female member, comprises a biasing member arranged to retain a respective sample plate in a female member and/or to bias the respective sample plate in a predetermined position. In one example, a sidewall portion comprises a recess, for example to facilitate inserting and/or removing the respective sample plate from the female member. In one example, the sample plater holder, for example each female member, comprises one or more seats (also known as pads) therein and/or thereon to receive a respective sample plate thereon. In this way, a height of the sample plate, for example of an upper surface thereof, may be predetermined for MS analysis, by controlling a height and/or a thickness of the seats, for example relative to an upper surface and/or a lower surface of the sample plate holder. In one example, a thickness, a flatness and/or a parallelism of the seats is within 0.05 mm, preferably within 0.04 mm, more preferably within 0.03 mm. In this way, a height of the first sample plate, for example of an upper surface thereof, may be arranged during MS analysis, for example for focusing a laser thereon and/or controlling a path length, or a part thereof, of the MS. In one example, the sample plate holder includes human-readable, for example alphanumeric, labels to identify positions of the female members by row and column.

That is, the operator may be instructed to place a particular sample plate in a particular female member. In one example, the sample plate holder includes no human-readable, for example alphanumeric, labels to identify positions of the female members by row and column. That is, a particular sample plate may be received in any of the female members. In this way, the operator is allowed to place any sample plate in any permissible placeholder of the sample plate holder, thereby eliminating a risk of accidental error and/or deliberate malicious actions in handling and/or placing of the sample plates, thereby excluding the risk of incorrect sample assignment and identification. In one example, a second portion of the upper surface, relatively more proximal a second end of the sample plate holder, mutually opposed to the first end, has no female members therein, for holding of the sample plate holder by an operator. In one example, the sample plate holder has an asymmetric shape, having a reduced number of planes of symmetry, for example a single plane of symmetry, or no planes of symmetry, orthogonal to an upper surface thereof In this way, the degrees of freedom for insertion of the sample plate holder into the MS is reduced, thereby guiding the operator to insert the sample plate holder therein in a predetermined orientation. In one example, the sample plate holder comprises a null UDI in a position corresponding to a position of an unique device identifier, UDI, included on a respective sample plate. In one example, one or more of the female members, for example all of the female members, include a null UDI in a position, for example a predetermined position, corresponding to the respective position of the first UDI of the first sample plate, wherein the null UDI indicates absence of a sample plate therein and/or thereon. In this way, the imager thus reads the null UDI and thereby absence of a sample plate is confirmed. Alternatively, the imager may attempt to image a UDI at a position, for example a predetermined position, corresponding to the respective position of the first UDI of the first sample plate, wherein a null imaging (i.e. not conforming with a UDI) indicates absence of a sample plate therein and/or thereon.

In one example, the sample plate holder is a multi-use sample plate holder, intended for reuse, for example following appropriate cleaning.

In one example, the sample plate holder includes a first identifier of a first set of identifiers. In this way, the sample plate holder may be identified, generally as described with respect to the first sample plate and the first UDI.

In one example, the first identifier is included, for example by printing, as a label, or engraving, in the second portion of the upper surface of the sample plate holder. Additionally and/or alternatively, in one example, the first identifier is included on the lower surface of the sample plate holder.

In one example, the first identifier comprises and/or is a visual code, for example a ID barcode or a 2D symbol, a magnetic strip, an RFID/NFC tag, an IC chip and/or a label. In contrast to sample plates, sample plate holders may not be classified as medical devices and hence the first identifier may not be required to conform to the same requirements as the first UDI, for example. In one example, the first identifier comprises and/or is a UDI. In this way, the first identifier may be read by the imager, as described with respect to the first UDI, mutafis mutandis.

In one example, the first identifier encodes information including one or more of: a type sample plate holder, a number of sample plates receivable therein and/or thereon and/or a type of sample plates receivable therein and/or thereon.

Sample plate holder The second aspect provides a sample plate holder having a first face and a reverse second face, wherein the first face comprises a set of holders, including a first holder, configured to hold a respective set of sample plates, including a first sample plate, therein and/or thereon, wherein the first sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the first holder comprises: a fulcrum defining a rotational axis of the first sample plate and delimiting a first region of the first holder to a first side of the fulcrum and a second region of the first holder to a mutually opposed second side of the fulcrum; and a set of releasable retaining members, including a first releasable retaining member, disposed to the first side of the fulcrum, for releasably retaining the first sample plate in a first rotational arrangement about the fulcrum, wherein the first surface of the first sample plate defines a datum plane.

The sample plate holder may be as described with respect to the first aspect. The sample plate may be as described with respect to the first aspect.

Kit comprising a sample plate sleeve and a sample plate The third aspect provides a kit comprising a sample plate sleeve and a sample plate, wherein the sample plate sleeve is configured to receive the sample plate therein and/or thereon, wherein the sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the sample plate sleeve comprises: a frame adapted to surround edges of the sample plate; and a fulcrum defining a rotational axis of the sample plate and delimiting a first region of the frame to a first side of the fulcrum and a second region of the frame to a mutually opposed second side of the fulcrum; wherein the sample plate is configurable in: a first rotational configuration about the fulcrum, wherein the first surface of the sample plate is recessed below the frame; and a second rotational configuration about the fulcrum, wherein the reverse second surface of the sample plate and the frame are mutually spaced apart to the first side of the fulcrum; wherein the sample plate is biased in the first rotational configuration and configured to move from the first configuration to the second configuration by applying a force on the first surface of the first sample plate to the second side of the fulcrum.

In this way, the first surface of the sample plate is protected by the frame in the first rotational configuration since the first surface of the sample plate is recessed therebelow. In this way, the sample plate sleeve facilitates handling of sample plates during sample deposition and/or storage before MALDI MS analysis, thereby avoiding contamination and/or destruction, for example, of the samples while preventing damage, for example bending, to the sample plate holder. Since the sample plate is arranged to move from the first rotational configuration to the second rotational configuration by applying the force on the first surface of the sample plate to the second side of the fulcrum, for example by an operator pressing the first surface using a finger, handling of the sample plate is facilitated, thereby avoiding contamination and/or destruction, for example, of the samples deposited on the sample plate. The sample plate may be then removed more readily by holding edges thereof that have rotated away from the first face of the sample plate holder. Conversely, in one example, the sample plate is configured to move from the second rotational configuration to the first rotational configuration by releasing a force on the first surface of the sample plate to the second side of the fulcrum, for example by an operator removing a finger from the first surface. For example, the sample plate may be inserted in the second rotational arrangement and subsequently, lowered into the first rotational arrangement by removing a finger from the first surface. Particularly, the inventors have identified that inserting and removing sample plates via pivoting thereof about a fulcrum facilitates handling, thereby avoiding contamination and/or destruction, for example, of the samples deposited on the sample plate. That is, the inventive concepts of the first aspect, the second aspect, the third aspect and the fourth aspect are the same. In contrast, conventionally, sample plates are not provided with any sleeve are instead loose, requiring careful handling by an operator. Hence, the kit according to the third aspect provides tool-free insertion and removal of sample plates while protecting samples deposited thereon.

A focus of the sample plate sleeve is to prevent bending or stress on the sample plates during shipment and provide a cost-effective way to house and contain the plates for handling. The sample plate sleeve is designed to meet this requirement.

The design of the sample plate is a consumable part so the design is tailored towards high-volume low-cost manufacturing of parts, for example by injection moulding of a polymeric composition comprising a thermoplastic polymer. Suitable polymeric compositions are known. A two-piece mould may be designed to have the parting line as centre as possible and with the addition of drafts, the touch to hand shouldn't feel any sharp.

Sample plate The sample plate may be as described with respect to the first aspect.

Sample plate sleeve The sample plate sleeve comprises the frame adapted to surround edges (i.e. a periphery) of the sample plate. In one example, the frame is adapted to surround at least 50%, preferably at least 75%, more preferably at least 90%, most preferably 100% of the edges of the sample plate, for example on one, two, three or four edges thereof, preferably on at least three edges thereof.

In this way, the edges of the sample plate are protected by the frame.

The sample plate sleeve comprises a fulcrum defining the rotational axis of the sample plate and delimiting the first region of the frame to the first side of the fulcrum and the second region of the frame to the mutually opposed second side of the fulcrum. The fulcrum, the rotational axis, the first region, the first side, second region and/or the second side may be as described with respect to the first aspect mutafis mutandis, description of which is not repeated for brevity.

In one example, the fulcrum is provided by an edge in the frame, for example by an edge of a bar or a base extending between opposite sides of the frame. In one example, the fulcrum is provided by pivot points at opposite sides of the frame.

Rotational configurations The sample plate is configurable in the first rotational configuration about the fulcrum, wherein the first surface of the sample plate is recessed below the frame. It should be understood that the first rotational configuration is for depositing samples on the sample plate and/or storage thereof.

The sample plate is configurable in the second rotational configuration about the fulcrum, wherein the reverse second surface of the sample plate and the frame are mutually spaced apart to the first side of the fulcrum. It should be understood that the second rotational configuration is for inserting or removing sample plates into the sample sleeve, for example for removing the sample plate therefrom for subsequent insertion into a sample plate holder, such as described with respect to the first aspect or a conventional sample plate holder. That is, it should be understood that while desirable to use a sample sleeve as described with respect to the third aspect and a sample plate holder as described with respect to the first aspect with a sample plate, such sample sleeve and such sample plate holder may be used independently.

The first rotational configuration and/or the second rotational configuration may be as described with respect to the first rotational arrangement and/or the second rotational arrangement of the first aspect mutatis mutandis, description of which is not repeated for brevity.

The sample plate is biased, for example rotationally due to a weight of the sample plate, in the first rotational configuration and configured to move from the first configuration to the second configuration by applying the force on the first surface of the first sample plate to the second side of the fulcrum, for example as described with respect to the first aspect mutatis mutandis, description of which is not repeated for brevity.

In one example, the frame is adapted to protrude above the first surface of the sample plate, in the first rotational configuration. In this way, samples deposited on the first surface of the sample plate are protected thereby. For example, the sample plate sleeve may be placed on a work surface such that the first surface of the sample plate is lowermost, without the first surface of the sample plate contacting the work surface which is instead contacted by the frame. For example, another sample plate sleeve may be stacked on the sample plate sleeve, without the first surface of the sample plate being contacted thereby. In one example, the frame is adapted to protrude below the second surface of the sample plate, in the first rotational configuration. In this way, the sample plate is protected thereby and/or the frame spaces apart a plurality of stacked sample plate sleeves.

In one example, the sample plate sleeve comprises a set of female members, for example in a lower side of the frame, and a set of corresponding male members, for example in an upper side of the frame, including a first female member and a corresponding first male member, for stacking a plurality of sample plate sleeves. In this way, a plurality of sample plate sleeves may be stacked regularly and interlocked by the set of female members and the corresponding male members.

In one example, the sample plate sleeve is configured to receive the sample plate therein and/or thereon in the second rotational configuration and by subsequently moving the sample plate from the first rotational configuration to the second rotational configuration. In this way, the sample plate is inserted into the sample plates leave in the second rotational configuration, for example by an operator, and subsequently moved from the first rotational configuration to the second rotational configuration, for example by releasing the sample plate whereby the sample plate rotates about the fulcrum into the second rotational arrangement. Removal of the sample plate is reversed.

In one example, the sample plate sleeve is configured to permit unidirectional sliding of the sample plate in the first rotational configuration, for example into a storage position wherein the frame and the sample plate mutually interlock. In this way, a lateral (e.g. x and/or y) position of the sample plate is defined by the sample sleeve in the first rotational arrangement.

In one example, the sample plate sleeve, for example the frame thereof, comprises one or more lips to secure the sample plate therein.

In one example, a plurality of kits are provided in a box, for example stacked.

Sample plate sleeve The fourth aspect provides a sample plate sleeve configured to receive a sample plate therein and/or thereon, wherein the sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the sample plate sleeve comprises: a frame adapted to surround edges of the sample plate; and a fulcrum defining a rotational axis of the sample plate and delimiting a first region of the frame to a first side of the fulcrum and a second region of the frame to a mutually opposed second side of the fulcrum.

The sample plate sleeve may be as described with respect to the third aspect. The sample plate may be as described with respect to the first aspect.

Definitions Throughout this specification, the term "comprising" or "comprises" means including the component(s) specified but not to the exclusion of the presence of other components. The term "consisting essentially of" or "consists essentially of" means including the components specified but excluding other components except for materials present as impurities, unavoidable materials present as a result of processes used to provide the components, and components added for a purpose other than achieving the technical effect of the invention, such as colourants, and the like. The term "consisting or or "consists of' means including the components specified but excluding other components. Whenever appropriate, depending upon the context, the use of the term "comprises" or "comprising" may also be taken to include the meaning "consists essentially or or "consisting essentially of', and also may also be taken to include the meaning "consists of' or "consisting of'. The optional features set out herein may be used either individually or in combination with each other where appropriate and particularly in the combinations as set out in the accompanying claims. The optional features for each aspect or exemplary embodiment of the invention, as set out herein are also applicable to all other aspects or exemplary embodiments of the invention, where appropriate. In other words, the skilled person imaging this specification should consider the optional features for each aspect or exemplary embodiment of the invention as interchangeable and combinable between different aspects and exemplary embodiments.

Brief description of the drawings

For a better understanding of the invention, and to show how exemplary embodiments of the same may be brought into effect, reference will be made, by way of example only, to the accompanying diagrammatic Figures, in which: Figure 1 schematically depicts a perspective view from above of an assembly, comprising a sample plate holder and a sample plate, arranged in a first rotational arrangement, according to an exemplary embodiment; Figure 2 is a CAD drawing of a perspective view from above of the sample plate holder of Figure 1; Figure 3 is a CAD drawing of an exploded perspective view from below of the sample plate holder of Figure 2; Figure 4 is a CAD drawing of a stage interface of a MS for the assembly of Figure 1; Figure 5 is a CAD drawing of a sectional perspective view from above of the assembly of Figure 1, arranged in a first rotational arrangement; Figure 6 is a CAD drawing of a perspective view from above of the assembly of Figure 1, arranged in a second rotational arrangement; Figure 7 is a CAD drawing of a cross-sectional view of the assembly of Figure 1, arranged in a first rotational arrangement; Figure 8 is a CAD drawing of a cross-sectional view of the assembly of Figure 1, arranged in a second rotational arrangement; Figure 9 is a CAD drawing of a plan view from above of the sample plate holder of Figure 1, in more detail; Figure 10 is a CAD drawing of a plan view from above, a plan view from below, a longitudinal cross-sectional view and a transverse cross-sectional view of the sample plate holder of Figure 1, in more detail; Figure 11 is a CAD drawing of a plan view from above of the sample plate of Figure 1, Figure 12 is a CAD drawing of a plan view from above, a longitudinal cross-sectional view and a transverse cross-sectional view of the sample plate of Figure 1, in more detail; Figure 13 is a CAD drawing of a perspective view from above of a kit, comprising a sample plate sleeve and a sample plate, configured in a first rotational configuration, according to an exemplary embodiment; Figure 14 is a CAD drawing of a perspective view from above of the sample plate sleeve of Figure 13; and Figure 15 is a CAD drawing of a perspective view from above of a stack of sample plate sleeves of Figure 13.

Detailed Description of the Drawings

Figure 1 schematically depicts a perspective view from above of an assembly A, comprising a sample plate holder 10 and a sample plate 1A, arranged in a first rotational arrangement, according to an exemplary embodiment; Figure 2 is a CAD drawing of a perspective view from above of the sample plate holder 10 of Figure 1; and Figure 3 is a CAD drawing of an exploded perspective view from below of the sample plate holder 10 of Figure 2. Figure 11 is a CAD drawing of a plan view from above of the sample plate 1A of Figure 1; and Figure 12 is a CAD drawing of a plan view from above, a longitudinal cross-sectional view and a transverse cross-sectional view of the sample plate 1A of Figure 1, in more detail.

The assembly A comprises a sample plate holder 10 having a first face 11 and a reverse second face 12, wherein the first face 11 comprises a set of holders 100, including a first holder 100A, and a respective set of sample plates 1, including a first sample plate 1A, wherein the set of holders 100, including the first holder 100A, is configured to hold the respective set of sample plate 1, including the first sample plate 1A, therein and/or thereon and wherein the first sample plate 1A has a planar first surface 1U for depositing samples thereon and a mutually parallel, reverse second surface 1L; wherein the first holder 100A comprises: a fulcrum 110 defining a rotational axis of the first sample plate 1A and delimiting a first region R1 of the first holder 100A to a first side Si of the fulcrum 110 and a second region R2 of the first holder 100A to a mutually opposed second side S2 of the fulcrum 110; and a set of releasable retaining members 120, including a first releasable retaining member 120A, disposed to the first side 51 of the fulcrum 110; wherein the first sample plate 1A is arrangeable in and/or on the first holder 100A in: a first rotational arrangement about the fulcrum 110, wherein the first sample plate 1A is releasably retained by the set of releasable retaining members 120 and wherein the first surface 1U defines a datum plane; and a second rotational arrangement about the fulcrum 110, wherein the first sample plate 1A is released from the set of releasable retaining members 120 and wherein the reverse second surface 1L of the first sample plate 1A and the first face 11 of the sample plate holder 10 are mutually spaced apart to the first side 51 of the fulcrum 110; wherein the first sample plate 1A is arranged to move from the first rotational arrangement to the second rotational arrangement by applying a force F on the first surface 1U of the first sample plate 1A to the second side S2 of the fulcrum 110.

In this example, the first sample plate 1A is arranged to move from the second rotational arrangement to the first rotational arrangement by releasing a force F on the first surface 1U of the first sample plate 1A to the second side 52 of the fulcrum 110, for example by an operator removing a finger from the first surface 1U.

In this example, the set of sample plate 1 includes 3 sample plates 1A, 1B, 1C. In this example, the first sample plate 1A is a MALDI MS sample plate.

In this example, the first sample plate 1A is a generally rectangular or square flat plate, having the planar first surface 1U for depositing samples thereon and the mutually parallel, reverse second surface 1L, formed from ferritic stainless steel, having an array, for example a regular array, of wells in a first portion of the first surface 1U thereof, relatively more proximal a first end of the first sample plate 1A, for depositing respective samples therein. In this example, the first sample plate 1A includes alphanumeric labels to identify positions of the wells by row and column. In this example, a second portion of the first surface 1U, relatively more proximal a second end of the first sample plate 1A, mutually opposed to the first end, has no wells therein, for holding of the first sample plate 1A by an operator. In this example, the first sample plate 1A has an asymmetric shape, having a reduced number of planes of symmetry, for example a single plane of symmetry, or no planes of symmetry, orthogonal to the first surface 1U. In this example, a first UDI is included, for example by printing, as a label, or engraving, in the second portion of the first surface 1U of the first sample plate 1A. In this example, the first sample plate 1A is a multi-use sample plate, intended for reuse, for example following appropriate cleaning. In this example, a thickness, a flatness and/or a parallelism of the first sample plate 1A is within 0.05 mm. In this example, the first UDI is a barcode. Each Holder has a barcode (Data Matrix) that is printed onto the holder. This barcode allows the software to identify the type of holder that is loaded into the instrument by scanning prior to analysis and thus determine the sample plate types being loaded into the instrument.

The overall dimensions of the sample plate holder 10 are: 149 mm length, 86 mm width, and a total height (thickness) of 5 mm.

In this example, the set of sample plate holders 100 includes 3 holders 100A, 100B, 100C. In this example, the first sample plate 1A holder 10 is a MALDI MS sample plate holder 10.

In this example, the sample plate holder 10 is a generally rectangular flat plate, wherein the first face 11 comprises and/or is a planar face and wherein the reverse second surface 1L is mutually parallel thereto, formed from stainless steel. In this example, the first face 11 comprises and/or is a planar face, defined by a set of mutually parallel planes, such as concavities therein. In this example, the reverse second face 12 comprises and/or is a planar face, for example defined by a single plane. In this example, the sample plate holder 10 includes human-readable, for example alphanumeric, labels to identify positions of the holders 100 by row and/or column.

In this example, the sample plate holder 10 has an asymmetric shape, having a single plane of symmetry orthogonal to the first face 11. In this example, a first UDI is included, for example by printing, as a label, or engraving, on the first face 11. In this example, the sample plate holder 10 is a multi-use sample plate holder 10, intended for reuse, for example following appropriate cleaning. In this example, a thickness, a flatness and/or a parallelism of the first face 11 and/or the second face 12 is within 0.05 mm.

In this example, the set of holders 100 is provided in the sample plate holder 10 such as in the first face 11, for example as a respective set of cavities in sample plate holder 10, for example machined or formed therein, optionally wherein respective perimeters (i.e. respective side walls) of the cavities have shapes corresponding with respective shapes of the sample plate 1, thereby constraining or preventing lateral movement thereof In this example, an uppermost surface of the assembly A is planar (i.e. defined by a single plane), thereby reducing or eliminating distortion of electric fields during MS.

In this example, the fulcrum 110 is provided by an edge in the first holder 100A, for example an edge E of a concavity or a step in the first holder 100A.

In this example, the first region R1 corresponds or substantially corresponds with a first portion of the first surface 1U of the first sample plate 1A having an array, for example a regular array, of wells in the first portion of the first surface 1U thereof, relatively more proximal a first end of the first sample plate 1A, for depositing respective samples therein. In this example, the second region R2 corresponds or substantially corresponds with a second portion of the first surface 1U of the first sample plate 1A, relatively more proximal a second end of the first sample plate 1A, mutually opposed to the first end, having no wells therein, for holding of the first sample plate 1A by an operator.

In this example, the first region R1 of the first holder 100A has a first depth relative to the first face 11 of the sample plate holder 10, wherein the second region R2 has a second depth relative to the first face 11 of the sample plate holder 10 and wherein the second depth is greater than the first depth.

In this example, a ratio of the first depth to the second depth is in a range from 9 10 to 1: 5, preferably in a range from 2: 3 to 1: 4, more preferably in a range from 1: 2 to 1: 3.

In this example, the first depth corresponds with a thickness of the first sample plate 1A.

In this example, the first region R1 comprises and/or is a planar region, for example defining a reference plane for the second surface 1L of the first sample plate 1A in the first rotational arrangement. In this example, the planar region is provided by a 1 x 1 array of pads 130, having a relief channel 140 therearound.

The difficult part of manufacturing the plate are the geometric tolerances relating to flatness and parallelism. Each of the islands where the sample plate sits on are datum pads, they are 50pm parallel to two islands at the back of the sample plate which is 50pm flat. The second high tolerance are the height between the datum at the bottom surface to each island, there is a 30pm tolerance to this. Also, corner relief have been made so a dog-bone feature allows the chamfer to sit as close to the sample plate as possible for the key in orientation.

The additional sheet metal plate on the second face 12 does not compromise the overall flatness and parallelism tolerance of the stage to sample plate interface, the location of the guard does not sit on the raised 0.5 mm datum pads on the stage tabletop giving design 0.5 mm allowable variation, this will be absorbed in the thickness tolerance and any height variation of the magnet, this would be approximately 0.3 mm.

In this example, the first region R1 has a first length orthogonal to the fulcrum 110, wherein the second region R2 has a second length orthogonal to the fulcrum 110 and wherein the first length is greater than the second length. In this way, the fulcrum 110 is disposed relatively more proximal a second end of the first holder 100A (i.e. the end of the second region R2) relative to a first end (i.e. the end of the first region R1) thereof. In this example, the magnitude of linear displacement of the first end is in a range from 10 mm to 15 mm.

In this example, a ratio of the first length to the second length is in a range from 2: 1 to 25 1, preferably in a range from 3: 1 to 15: 1, more preferably in a range from 5: 1 to 10: 1.

The first holder 100A comprises the set of releasable retaining members 120, including the first releasable retaining member 120A (i.e. at least one releasable retaining member), disposed to the first side Si of the fulcrum 110. In this example, the set of releasable retaining members 120, including the first releasable retaining member 120A, is disposed only to the first side Si of the fulcrum 110. In this example, the first holder 100A does not comprise a releasable retaining member disposed to the second side S2 of the fulcrum 110.

In this example, the first releasable retaining member 120A comprises and/or is a magnet (such as provided in the first region R1, for example under the first sample plate 1A). In this example, the set of releasable retaining members 120 includes a second releasable retaining member 120B. In this example, the magnets sit in counter bore pockets and are then clamped into place using 0.5 mm stainless sheet metal. Uses a countersunk vented screw M2 x 3 to hold it in place.

In this example, the first releasable retaining member 120A is disposed at a first distance orthogonal to the fulcrum 110, wherein the second releasable retaining member 120A is disposed at a second distance orthogonal to the fulcrum 110, wherein the second distance is greater than the first distance and wherein a first force F required to release the first releasable retaining member 120A is greater than a second force F required to release the second releasable retaining member 120A.

In this example, the first sample plate lA is rotatable by an angle in a range from 7° to 15°, for example 10° about the fulcrum 110.

In this example, the first holder 100A is arranged to receive the first sample plate 1A therein and/or thereon in the second rotational arrangement and by subsequently moving the first sample plate 1A from the first rotational arrangement to the second rotational arrangement.

In this example, the first holder 100A is adapted to limit sliding, for example parallel to the datum plane, of the first sample plate lA in the first arrangement.

In this example, the sample plate holder 10 is a generally rectangular or square flat plate, for example having mutually parallel upper and lower surfaces, formed from stainless steel or a polymeric composition comprising a polymer having a conductive coating thereon for example, having an array, for example a regular array, of female members (also known as placeholders 100) in and/or on a first portion of an upper surface thereof, relatively more proximal a first end of the sample plate holder 10, for receiving respective sample plate 1 therein. In this example, each female member has a shape corresponding with a shape, for example an asymmetric shape, of a respective sample plate. In this example, each female member comprises a plurality of sidewall portions arranged to retain a respective sample plate in one or two dimensions. In this example, the sample plate holder 10, for example each female member, comprises a biasing member arranged to retain a respective sample plate in a female member and/or to bias the respective sample plate in a predetermined position. In this example, a sidewall portion comprises a recess, for example to facilitate inserting and/or removing the respective sample plate from the female member. In this example, the sample plater holder, for example each female member, comprises one or more seats (also known as pads) therein and/or thereon to receive a respective sample plate thereon. In this way, a height of the sample plate, for example of an upper surface thereof, may be predetermined for MS analysis, by controlling a height and/or a thickness of the seats, for example relative to an upper surface and/or a lower surface of the sample plate holder 10. In this example, a thickness, a flatness and/or a parallelism of the seats is within 0.05 mm, preferably within 0.04 mm, more preferably within 0.03 mm. In this way, a height of the first sample plate 1A, for example of an upper surface thereof, may be arranged during MS analysis, for example for focusing a laser thereon and/or controlling a path length, or a part thereof, of the MS. In this example, the sample plate holder 10 includes human-readable, for example alphanumeric, labels to identify positions of the female members by row and column. That is, the operator may be instructed to place a particular sample plate in a particular female member. In this example, the sample plate holder 10 includes no human-readable, for example alphanumeric, labels to identify positions of the female members by row and column. That is, a particular sample plate may be received in any of the female members. In this way, the operator is allowed to place any sample plate in any permissible placeholder of the sample plate holder 10, thereby eliminating a risk of accidental error and/or deliberate malicious actions in handling and/or placing of the sample plate 1, thereby excluding the risk of incorrect sample assignment and identification. In this example, a second portion of the upper surface, relatively more proximal a second end of the sample plate holder 10, mutually opposed to the first end, has no female members therein, for holding of the sample plate holder 10 by an operator. In this example, the sample plate holder 10 has an asymmetric shape, having a reduced number of planes of symmetry, for example a single plane of symmetry, or no planes of symmetry, orthogonal to an upper surface thereof In this way, the degrees of freedom for insertion of the sample plate holder 10 into the MS is reduced, thereby guiding the operator to insert the sample plate holder 10 therein in a predetermined orientation. In this example, the sample plate holder 10 comprises a null UDI in a position corresponding to a position of an unique device identifier, UDI, included on a respective sample plate. In this example, one or more of the female members, for example all of the female members, include a null UDI in a position, for example a predetermined position, corresponding to the respective position of the first UDI of the first sample plate 1A, wherein the null UDI indicates absence of a sample plate therein and/or thereon. In this way, the imager thus reads the null UDI and thereby absence of a sample plate is confirmed. Alternatively, the imager may attempt to image a UDI at a position, for example a predetermined position, corresponding to the respective position of the first UDI of the first sample plate 1A, wherein a null imaging (i.e. not conforming with a UDI) indicates absence of a sample plate therein and/or thereon.

In this example, the sample plate holder 10 is a multi-use sample plate holder 10, intended for reuse, for example following appropriate cleaning.

In this example, the sample plate holder 10 includes a first identifier of a first set of identifiers. In this way, the sample plate holder 10 may be identified, generally as described with respect to the first sample plate 1A and the first UDI.

In this example, the first identifier is included, for example by printing, as a label, or engraving, in the second portion of the upper surface of the sample plate holder 10. Additionally and/or alternatively, In this example, the first identifier is included on the lower surface of the sample plate holder 10.

In this example, the first identifier comprises and/or is a visual code, for example a 1D barcode or a 2D symbol, a magnetic strip, an RFID/NFC tag, an IC chip and/or a label. In contrast to sample plate 1, sample plate holders may not be classified as medical devices and hence the first identifier may not be required to conform to the same requirements as the first UDI, for example. In this example, the first identifier comprises and/or is a UDI. In this way, the first identifier may be read by the imager, as described with respect to the first UDI, mutafis mutandis. In this example, the first identifier encodes information including one or more of a type sample plate holder 10, a number of sample plate 1 receivable therein and/or thereon and/or a type of sample plate 1 receivable therein and/or thereon.

In this example, the first sample plate holder 10A is arranged to hold a subset of a set of sample plates 1, wherein the subset includes N sample plates, wherein N is 3. In this example, three first sample plates 1A, 1B, 1C, are held in the first sample plate holder 10.

In this example, the first sample plate 1A is held in and/or on a first sample plate holder 10A (also known as a carrier) of a set of sample plate holders. In this example, the first sample plate holder 10A is arranged to hold a subset of up to 3 sample plates therein and/or thereon, by having a corresponding number of female members particularly machined concavities for receiving the subset of sample plates therein.

In this example, the first sample plate holder 10A is a generally rectangular or square flat plate, formed from stainless steel, having a regular array of 1 x 3 female members in and/or on a first portion of an upper surface thereof, relatively more proximal a first end of the first sample plate holder 10A, for holding respective sample plates 1 therein. In this example, each female member has a shape corresponding with a shape, for example an asymmetric shape, of a respective sample plate. In this example, the first sample plate holder 10A includes no human-readable, for example alphanumeric, labels to identify positions of the female members by row and column. In this example, a second portion of the upper surface, relatively more proximal a second end of the first sample plate holder 10A, mutually opposed to the first end, has no female members therein, for holding of the first sample plate holder 10A by an operator. In this example, the first sample plate holder 10A has an asymmetric shape, having a reduced number of planes of symmetry, having a single plane of symmetry orthogonal to the upper surface.

Additional marking is in the design to reduce chance of inserting the plate in the wrong orientation, two arrows per sample holder pocket will match up to the sample plate where they point to each other. This gives a visual confirmation to the user that the sample plate is inserted in the right orientation. Also, a barcode for identification of the type of holder is printed on the front of each sample holder containing the UDI.

The first sample plate 1A is in a Ox 14 layout. This is the mechanical properties of this plate: * The material is 430 grade Stainless Steel (Ferrific Grade) * The size is 30 mm x 75 mm * The thickness is 0.3 ± 0.03 mm. The flatness and parallelism between top and bottom faces is 30 pm.

* Have chamfers in the top left corners to prevent incorrect placement onto the holder.

* Positional accuracy of the spot is 50 pm diameter from the Theoretical exact axis.

* This plate has UDI in the form of Datamatrix symbol on upper surface together with human readable version * The plate contains 84 sample spots with diameter 2.4 ± 0.03 mm.

* These plates also have two pointers at the top and at left hand side to avoid the incorrect location on sample plate holder.

Figure 4 is a CAD drawing of a stage interface of a MS for the assembly A of Figure 1, having sensors for location magnets Ml, M2 provided on second face 12 of the sample plate 10.

Figure 5 is a CAD drawing of a sectional perspective view from above of the assembly A of Figure 1, arranged in a first rotational arrangement, wherein the first sample plate 1A is releasably retained by the set of releasable retaining members 120 and wherein the first surface 1U defines a datum plane.

Figure 6 is a CAD drawing of a perspective view from above of the assembly A of Figure 1, arranged in a second rotational arrangement, wherein the first sample plate 1A is released from the set of releasable retaining members 120 and wherein the reverse second surface 1 L of the first sample plate 1A and the first face 11 of the sample plate holder 10 are mutually spaced apart to the first side of the fulcrum 110.

A lever point near the stronger of the two magnets allow user to push and remove the sample plate, the two magnets that hold the plate are difference size and pull force to provide a 60% to 40% ratio. this is to make the leverage easier while maintaining enough force to keep the sample plate in the pocket and keeping it flat. Sample plate pivot to angle of 10 degrees, giving user llmm height to grab onto it. Thinner magnet held in position with spacers.

6mm x 2mm N42 magnet at 1.2mm space, pull force is 0.11kgf * 6mm x 3mm N42 magnet at 1.2mm space, pull force is 0.18kgf * Total pull force 0.29kgf Now the holding moment 0.11"0.05+0.18*0.0038=F*0.0095, F=0.65kgf Below shows calculation of the maximum stress at the pivot point, it is far below yield point hence, it wouldn't permanently deform the sample plate. Mc

all F.C1,3Itt c = h/2 = 0.5 0.0003 = 0.00015 M=Fxd= 0.65.0.0095 = 6.1751.0-13 Mc = 6.17510-3 x 0.00015 = 9.26 x 10-7 b10 plate 0.3npa thk 30nErn v I = 525 x10-3.4 (ThcErtd,"."xx = 1.37 x 10 Pa = 13.7114Pa (Yield of 3165S is a /Wilt 200htpc) Figure 7 is a CAD drawing of a cross-sectional view of the assembly A of Figure 1, arranged in a first rotational arrangement.

Figure 8 is a CAD drawing of a cross-sectional view of the assembly A of Figure 1, arranged in a second rotational arrangement.

Figure 9 is a CAD drawing of a plan view from above of the sample plate holder 10 of Figure 1, in more detail.

Figure 10 is a CAD drawing of a plan view from above, a plan view from below, a longitudinal cross-sectional view and a transverse cross-sectional view of the sample plate holder 10 of Figure 1, in more detail.

Figure 11 is a CAD drawing of a plan view from above of the sample plate 1A of Figure 1; and Figure 12 is a CAD drawing of a plan view from above, a longitudinal cross-sectional view and a transverse cross-sectional view of the sample plate 1A of Figure 1, in more detail. In this example, the first sample plate 1A is a generally rectangular flat plate, formed ferritic stainless steel, having a regular 14 x 6 array of wells in a first portion of an upper surface thereof, relatively more proximal a first end of the first sample plate 1A, for deposition of respective samples S therein. In this example, the first sample plate 1A includes human-readable, for example alphanumeric, labels (not shown) to identify positions of the wells by row and column. In this example, a second portion of the upper surface, relatively more proximal a second end of the first sample plate 1A, mutually opposed to the first end, has no wells therein, for holding of the first sample plate 1A by an operator. In this example, the first sample plate 1A has an asymmetric shape, having a reduced number of planes of symmetry, having a single plane of symmetry orthogonal to the upper surface. In this example, the first UDI Ul A is included, for example by printing, as a label, or engraving, in the second portion of the upper surface of the first sample plate 1A. In this example, the first sample plate 1A is a single-use sample plate, not intended for reuse. In this example, the first UDI U1A is a 2D barcode such as a QR code or a Data Matrix.

Figure 13 is a CAD drawing of a perspective view from above of a kit K, comprising a sample plate sleeve 20 and a sample plate 1, configured in a first rotational configuration, according to an exemplary embodiment; Figure 14 is a CAD drawing of a perspective view from above of the sample plate sleeve 20 of Figure 13; and Figure 15 is a CAD drawing of a perspective view from above of a stack of sample plate sleeve 20 (20A, 20B, 20C, 20D, 20E, 20F) of Figure 13.

The kit K comprises a sample plate sleeve 20 and a sample plate 1, wherein the sample plate sleeve 20 is configured to receive the sample plate 1 therein and/or thereon, wherein the sample plate 1 has a planar first surface 1U for receiving samples thereon and a mutually parallel, reverse second surface 1L; wherein the sample plate sleeve 20 comprises: a frame 230 adapted to surround edges of the sample plate 1; and a fulcrum 210 defining a rotational axis of the sample plate 1 and delimiting a first region R1 of the frame 230 to a first side 81 of the fulcrum 210 and a second region R2 of the frame 230 to a mutually opposed second side 82 of the fulcrum 210; wherein the sample plate 1 is configurable in: a first rotational configuration about the fulcrum 210, wherein the first surface 1U of the sample plate 1 is recessed below the frame 230; and a second rotational configuration about the fulcrum 210, wherein the reverse second surface 1L of the sample plate 1 and the frame 230 are mutually spaced apart to the first side S1 of the fulcrum 210; wherein the sample plate 1 is biased in the first rotational configuration and configured to move from the first configuration to the second configuration by applying a force F on the first surface 1U of the first sample plate 1 1A to the second side S2 of the fulcrum 210.

In this example, the sample plate 1 is configured to move from the second rotational configuration to the first rotational configuration by releasing a force F on the first surface 1U of the sample plate 1 to the second side 82 of the fulcrum 210, for example by an operator removing a finger from the first surface 1U.

The sample plate sleeve 20 comprises the frame 230 adapted to surround edges (i.e. a periphery) of the sample plate 1. In this example, the frame 230 is adapted to surround at least at least 90% of the edges of the sample plate 1, for example on three edges thereof In this example, the fulcrum 210 is provided by an edge in the frame 230, for example by an edge of a bar or a base 231 extending between opposite sides of the frame 230.

In this example, the frame 230 is adapted to protrude above the first surface 1U of the sample plate 1, in the first rotational configuration. In this example, the frame 230 is adapted to protrude below the second surface 1L of the sample plate 1, in the first rotational configuration.

In this example, the sample plate sleeve 20 comprises a set of female members 232 (232A, 232B), for example in a lower side of the frame 230, and a set of corresponding male members 233 (233A, 233B), for example in an upper side of the frame 230, including a first female member 232A and a corresponding first male member 233A, for stacking a plurality of sample plate sleeves 20.

In this example, the sample plate sleeve 20 is configured to receive the sample plate 1 therein and/or thereon in the second rotational configuration and by subsequently moving the sample plate 1 from the first rotational configuration to the second rotational configuration.

In this example, the sample plate sleeve 20 is configured to permit unidirectional sliding of the sample plate 1 in the first rotational configuration, for example into a storage position wherein the frame 230 and the sample plate 1 mutually interlock.

In this example, the sample plate sleeve 20, for example the frame 230 thereof, comprises one or more lips 234A, 234B to secure the sample plate therein.

Figure 15 is a CAD drawing of a perspective view from above of a stack of sample plate sleeves 20 (20A, 20B, 20C, 20D, 20E, 20F) of Figure 13. Each sample plate sleeve holds a respective sample plate, which are mutually spaced apart by the sample plate sleeves 20, thereby preventing damage thereto.

Alternatives Although a preferred embodiment has been shown and described, it will be appreciated by those skilled in the art that various changes and modifications might be made without departing from the scope of the invention, as defined in the appended claims and as described above. Attention is directed to all papers and documents which are filed concurrently with or previous to this specification in connection with this application and which are open to public inspection with this specification, and the contents of all such papers and documents are incorporated herein by reference. All of the features disclosed in this specification (including any accompanying claims and drawings), and/or all of the steps of any method or process so disclosed, may be combined in any combination, except combinations where at most some of such features and/or steps are mutually exclusive. Each feature disclosed in this specification (including any accompanying claims, and drawings) may be replaced by alternative features serving the same, equivalent or similar purpose, unless expressly stated otherwise. Thus, unless expressly stated otherwise, each feature disclosed is one example only of a generic series of equivalent or similar features. The invention is not restricted to the details of the foregoing embodiment(s). The invention extends to any novel one, or any novel combination, of the features disclosed in this specification (including any accompanying claims and drawings), or to any novel one, or any novel combination, of the steps of any method or process so disclosed.

Claims

CLAIMS1. An assembly comprising a sample plate holder having a first face and a reverse second face, wherein the first face comprises a set of holders, including a first holder, and a respective set of sample plates, including a first sample plate, wherein the set of holders, including the first holder, is configured to hold the respective set of sample plates, including the first sample plate, therein and/or thereon and wherein the first sample plate has a planar first surface for depositing samples thereon and a mutually parallel, reverse second surface; wherein the first holder comprises: a fulcrum defining a rotational axis of the first sample plate and delimiting a first region of the first holder to a first side of the fulcrum and a second region of the first holder to a mutually opposed second side of the fulcrum; and a set of releasable retaining members, including a first releasable retaining member 120A, disposed to the first side of the fulcrum; wherein the first sample plate is arrangeable in and/or on the first holder in: a first rotational arrangement about the fulcrum, wherein the first sample plate is releasably retained by the set of releasable retaining members and wherein the first surface defines a datum plane; and a second rotational arrangement about the fulcrum, wherein the first sample plate is released from the set of releasable retaining members and wherein the reverse second surface of the first sample plate and the first face of the sample plate holder are mutually spaced apart to the first side of the fulcrum; wherein the first sample plate is arranged to move from the first rotational arrangement to the second rotational arrangement by applying a force on the first surface of the first sample plate to the second side of the fulcrum.
2. The assembly according to claim 1, wherein the fulcrum is provided by an edge in the first holder.
3. The assembly according to any previous claim, wherein the first region of the first holder has a first depth relative to the first face of the sample plate holder, wherein the second region has a second depth relative to the first face of the sample plate holder and wherein the second depth is greater than the first depth.
4. The assembly according to claim 3, wherein a ratio of the first depth to the second depth is in a range from 9: 10 to 1: 5, preferably in a range from 2: 3 to 1: 4, more preferably in a range from 1: 2 to 1: 3.
5. The assembly according to any of claims 3 to 4, wherein the first depth corresponds with a thickness of the first sample plate.
6. The assembly according to any previous claim, wherein the first region comprises and/or is a planar region.
7. The assembly according to any previous claim, wherein the first region has a first length orthogonal to the fulcrum, wherein the second region has a second length orthogonal to the fulcrum and wherein the first length is greater than the second length.
8. The assembly according to claim 7, wherein a ratio of the first length to the second length is in a range from 2: 1 to 25: 1, preferably in a range from 3: 1 to 15: 1, more preferably in a range from 5: 1 to 10: 1.
9. The assembly according to any previous claim, wherein the first releasable retaining member 120A comprises and/or is a magnet, a resilient biasing member, a cam and/or a latch.
10. The assembly according to any previous claim, wherein the set of releasable retaining members includes a second releasable retaining member. 20
11. The assembly according to any previous claim, wherein the first releasable retaining member 120A is disposed at a first distance orthogonal to the fulcrum, wherein the second releasable retaining member is disposed at a second distance orthogonal to the fulcrum, wherein the second distance is greater than the first distance and wherein a first force required to release the first releasable retaining member 120A is greater than a second force required to release the second releasable retaining member.
12. The assembly according to any previous claim, wherein the first holder is arranged to receive the first sample plate therein and/or thereon in the second rotational arrangement and by subsequently moving the first sample plate from the first rotational arrangement to the second rotational arrangement.
13. The assembly according to any previous claim, wherein the first holder is adapted to limit sliding of the first sample plate in the first arrangement.
14. A sample plate holder having a first face and a reverse second face, wherein the first face comprises a set of holders, including a first holder, configured to hold a respective set of sample plates, including a first sample plate, therein and/or thereon, wherein the first sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the first holder comprises: a fulcrum defining a rotational axis of the first sample plate and delimiting a first region of the first holder to a first side of the fulcrum and a second region of the first holder to a mutually opposed second side of the fulcrum; and a set of releasable retaining members, including a first releasable retaining member 120A, disposed to the first side of the fulcrum, for releasably retaining the first sample plate in a first rotational arrangement about the fulcrum, wherein the first surface of the first sample plate defines a datum plane.
15. A kit comprising a sample plate sleeve and a sample plate, wherein the sample plate sleeve is configured to receive the sample plate therein and/or thereon, wherein the sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the sample plate sleeve comprises: a frame adapted to surround edges of the sample plate; and a fulcrum defining a rotational axis of the sample plate and delimiting a first region of the frame to a first side of the fulcrum and a second region of the frame to a mutually opposed second side of the fulcrum; wherein the sample plate is configurable in: a first rotational configuration about the fulcrum, wherein the first surface of the sample plate is recessed below the frame; and a second rotational configuration about the fulcrum, wherein the reverse second surface of the sample plate and the frame are mutually spaced apart to the first side of the fulcrum; wherein the sample plate is biased in the first rotational configuration and configured to move from the first configuration to the second configuration by applying a force on the first surface of the first sample plate to the second side of the fulcrum.
16. The kit according to claim 15, wherein the frame is adapted to protrude above the first surface of the sample plate and/or below the second surface of the sample plate, in the first rotational configuration.
17. The kit according to any of claims 15 to 16, wherein the sample plate sleeve comprises a set of female members and a set of corresponding male members, including a first female member and a corresponding first male member, for stacking a plurality of sample plate sleeves.
18. The kit according to any of claims 15 to 17, wherein the sample plate sleeve is configured to receive the sample plate therein and/or thereon in the second rotational configuration and by subsequently moving the sample plate from the first rotational configuration to the second rotational configuration.
19. The kit according to any of claims 15 to 18, wherein the sample plate sleeve is configured to permit unidirectional sliding of the sample plate in the first rotational configuration.
20. A sample plate sleeve configured to receive a sample plate therein and/or thereon, wherein the sample plate has a planar first surface for receiving samples thereon and a mutually parallel, reverse second surface; wherein the sample plate sleeve comprises: a frame adapted to surround edges of the sample plate; and a fulcrum defining a rotational axis of the sample plate and delimiting a first region of the frame to a first side of the fulcrum and a second region of the frame to a mutually opposed second side of the fulcrum.