GB2604472A - Analyte detection method and device - Google Patents
Analyte detection method and device Download PDFInfo
- Publication number
- GB2604472A GB2604472A GB2206029.7A GB202206029A GB2604472A GB 2604472 A GB2604472 A GB 2604472A GB 202206029 A GB202206029 A GB 202206029A GB 2604472 A GB2604472 A GB 2604472A
- Authority
- GB
- United Kingdom
- Prior art keywords
- analyte
- detection device
- interest
- fused
- peroxidase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present disclosure relates to a novel analyte detection device and methods for detecting analytes of interest, such as allergens. In particular, the present disclosure provides a portable analyte detection device that is intended for on-site use by consumers. A fused split peroxidase-analyte binder complex is disclosed having a split peroxidase in at least two fragments, each fused to an analyte binder fragment capable of binding an analyte of interest.
Claims (19)
1. A fused split peroxidase-analyte binder complex comprising a split peroxidase, wherein the split peroxidase is at least two fragments, where each fragment is fused, optionally through a linker, to an analyte binder fragment consisting of a peptide or protein of interest which is capable of binding an analyte of interest, wherein the analyte is an allergen.
2. The fused split peroxidase-analyte binder complex of claim 1, wherein the split peroxidase is an ascorbic peroxidase.
3. The fused split peroxidase-analyte binder complex of claim 1, wherein the allergen of interest is a food allergen.
4. The fused split peroxidase-analyte binder complex of claim 3, wherein the food allergen is gluten and the analyte binder is a peptide that is capable of binding gluten.
5. An analyte detection device for detecting an analyte of interest in a sample which comprises: a fused split peroxidase-analyte binder complex comprising a split peroxidase, wherein the split peroxidase is at least two fragments, where each fragment is fused, optionally through a linker, to an analyte binder fragment consisting of a peptide or protein of interest, which is capable of binding an analyte of interest; and a substrate that is capable of undergoing catalysis by the fused split peroxidase- analyte binder complex once reconstituted in the presence of an analyte of interest, wherein the catalysis of the substrate is detectable.
6. The analyte detection device of claim 5, further comprising one or more buffers, and optional reagents.
7. The analyte detection device of claim 5, wherein the split peroxidase is an ascorbic peroxidase and the substrate is ascorbate.
8. The analyte detection device of claim 5, wherein the analyte of interest is an allergen.
9. The analyte detection device of claim 8, wherein the allergen is a food allergen.
10. The analyte detection device of claim 9, wherein the food allergen is gluten and the analyte binder is a peptide that is capable of binding gluten.
11. The analyte detection device of claim 5, wherein the catalysis of the substrate results in a colorimetric or fluorogenic signal which is detectable.
12. The analyte detection device of claim 5, wherein the device comprises: a sample collection apparatus; and a reaction vessel comprising the fused split peroxidase-analyte binder complex and the substrate.
13. The analyte detection device of claim 12, wherein the reaction vessel comprises: a casing; one or more compartments; and a container.
14. The analyte detection device of claim 13, wherein the reaction vessel contains a reaction viewing window.
15. The analyte detection device of claim 13, wherein the reaction vessel further comprises an absorbent material.
16. Use of the fused split peroxidase-analyte binder complex of any one of claims 1 - 4 for detection of an allergen in a sample.
17. The use of claim 16, wherein the sample is a food sample.
18. Use of the analyte detection device of any one of claims 5 - 14 for detection of an analyte of interest in a sample.
19. The use of claim 18, wherein the sample is a food sample.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962907101P | 2019-09-27 | 2019-09-27 | |
| PCT/US2020/052902 WO2021062291A1 (en) | 2019-09-27 | 2020-09-25 | Analyte detection method and device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB202206029D0 GB202206029D0 (en) | 2022-06-08 |
| GB2604472A true GB2604472A (en) | 2022-09-07 |
Family
ID=75166861
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2206029.7A Pending GB2604472A (en) | 2019-09-27 | 2020-09-25 | Analyte detection method and device |
Country Status (3)
| Country | Link |
|---|---|
| CA (1) | CA3152627A1 (en) |
| GB (1) | GB2604472A (en) |
| WO (1) | WO2021062291A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023119246A1 (en) | 2021-12-24 | 2023-06-29 | Softcar Sa | Automobile plant with small carbon footprint |
| WO2024072897A1 (en) * | 2022-09-28 | 2024-04-04 | The Regents Of The University Of Michigan | Peroxidase-based reporters to detect protease activity and protein interactions |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9012170B2 (en) * | 2013-01-16 | 2015-04-21 | Massachusetts Institute Of Technology | Split peroxidases and methods of use |
| WO2017160616A1 (en) * | 2016-03-15 | 2017-09-21 | Dots Technology Corp. | Systems and methods for allergen detection |
| WO2017189751A1 (en) * | 2016-04-26 | 2017-11-02 | The University Of Utah Research Foundation | Target-binding activated split reporter systems for analyte detection and related components and methods |
-
2020
- 2020-09-25 GB GB2206029.7A patent/GB2604472A/en active Pending
- 2020-09-25 CA CA3152627A patent/CA3152627A1/en active Pending
- 2020-09-25 WO PCT/US2020/052902 patent/WO2021062291A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9012170B2 (en) * | 2013-01-16 | 2015-04-21 | Massachusetts Institute Of Technology | Split peroxidases and methods of use |
| WO2017160616A1 (en) * | 2016-03-15 | 2017-09-21 | Dots Technology Corp. | Systems and methods for allergen detection |
| WO2017189751A1 (en) * | 2016-04-26 | 2017-11-02 | The University Of Utah Research Foundation | Target-binding activated split reporter systems for analyte detection and related components and methods |
Non-Patent Citations (1)
| Title |
|---|
| LAD et al., Substrate Binding and Catalytic Mechanism in Ascorbate Peroxidase: Evidence for Two Ascorbate Binding Sites. Biochemistry, 19 November 2002, Vol. 41, No.46, pg 13774 - 3781; abstract * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021062291A1 (en) | 2021-04-01 |
| CA3152627A1 (en) | 2021-04-01 |
| GB202206029D0 (en) | 2022-06-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Vernozy‐Rozand et al. | Comparison of three immunological methods for detecting staphylococcal enterotoxins from food | |
| GB2604472A (en) | Analyte detection method and device | |
| WO2007075928A3 (en) | Integrated patch and assay device with visual detection means | |
| AU4073297A (en) | Methods and compositions for the detection of cervical cancer | |
| MX2009004460A (en) | Immunoassay of analytes in samples containing endogenous anti-analyte antibodies. | |
| JO3812B1 (en) | Antibodies that bind to human programmed death ligand 1 (pd-l1) | |
| WO2001036470A3 (en) | Methods and compositions for detection and treatment of breast cancer, based on breast cancer-associated polypeptides | |
| WO2009074276A3 (en) | Marker panel for colorectal cancer | |
| WO2006026020A3 (en) | Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin | |
| GB2374415B (en) | Human antibodies as detection reagents | |
| AU7441498A (en) | Immunoassay apparatus for diagnosis | |
| WO2012142623A3 (en) | Antigen detection system and methods of use | |
| AU2003304573A1 (en) | Methods and device for analyte characterization | |
| AU2003224447A1 (en) | Device and method to simultaneously detect different antibodies and antigens in clinical alimentary and environmental samples | |
| Harder et al. | Surface acoustic wave-based microfluidic coagulation device for monitoring anticoagulant therapy | |
| WO2006004958A3 (en) | Detection or measurement of antibodies to antigenic proteins in biological tissues or samples | |
| HK1080091A1 (en) | Immunoassays for specific determination of scca isoforms | |
| EP1591791A3 (en) | Diagnostic assay for human matrix gla-protein and its use as a biomarker | |
| JP2004536295A5 (en) | ||
| WO2007027919A3 (en) | Complementation assays utilizing complexes of heteroproteins | |
| ATE413472T1 (en) | ANALYSIS CHIP WITH REFERENCE SCALE KITS AND ANALYSIS METHODS | |
| WO2003029813A3 (en) | Test strips for the detection of prion proteins | |
| WO2004057301A3 (en) | Isolation and confirmation of analytes from test devices | |
| EP1674870A3 (en) | High-throughput prion assays | |
| Chernukha et al. | Qualimetric assessment of methods for the determination of allergens in meat products |