GB2597287A - Use of cannabidiol in the treatment of seizures associated with rare epilepsy syndromes related to structural abnormalities of the brain - Google Patents

Abstract

The use of a cannabidiol (CBD) preparation for the treatment of seizures associated with rare epilepsy syndromes, in particular perisylvian fissure syndrome. The seizures associated with perisylvian fissure syndrome include focal seizures with impairment. The CBD preparation may comprise 95% (w/w) CBD and not more than 0.15% (w/w) tetrahydrocannabinol (THC). Preferably the CBD preparation comprises greater than or equal to 98% (w/w) CBD and less than or equal to 2% (w/w) other cannabinoids, which may comprise THC, cannabidiol-C1 (CBC-C1), cannabidivarin (CBDV), and cannabidiol-C4 (CBD-C4), where the THC may be a mixture of trans-THC and cis-THC. The CBD preparation may be used in combination with one or more concomitant anti-epileptic drugs (AEDs) such as levetiracetam, clonazepam, diazepam, and lacosamide. The CBD in the CBD preparation may be synthetic or isolated from cannabis plant material. Preferably the dose of CBD is between 5 mg/kg/day to 50 mg/kg/day.

Description

USE OF CANNABIDIOL IN THE TREATMENT OF SEIZURES ASSOCIATED WITH RARE

EPILEPSY SYNDROMES RELATED TO STRUCTURAL ABNORMALITIES OF THE BRAIN

FIELD OF THE INVENTION

[0001] The present invention relates to the use of cannabidiol (CBD) for the treatment of seizures associated with rare epilepsy syndromes. In particular the seizures associated with rare epilepsy syndromes that are treated are those which are experienced in patients diagnosed with perisylvian fissure syndrome. In a further embodiment the types of seizures include focal seizures with impairment. Preferably the dose of CBD is between 5 mg/kg/day to mg/kg/day.

[0002] In a further embodiment the CBD used is in the form of a highly purified extract of cannabis such that the CBD is present at greater than 95% of the total extract (w/w) and the cannabinoid tetrahydrocannabinol (THC) has been substantially removed, to a level of not more than 0.15% (w/w).

[0003] Preferably the CBD used is in the form of a botanically derived purified CBD which comprises greater than or equal to 98% (w/w) CBD and less than or equal to 2% (w/w) of other cannabinoids. More preferably the other cannabinoids present are THC at a concentration of less than or equal to 0.1% (w/w); CBD-C1 at a concentration of less than or equal to 0.15% (w/w); CBDV at a concentration of less than or equal to 0.8% (w/w); and CBD-C4 at a concentration of less than or equal to 0.4% (w/w). The botanically derived purified CBD preferably also comprises a mixture of both trans-THC and cis-THC. Alternatively, a synthetically produced CBD is used.

[0004] Where the CBD is given concomitantly with one or more other anti-epileptic drugs (AED), the CBD may be formulated for administration separately, sequentially or simultaneously with one or more AED or the combination may be provided in a single dosage form.

BACKGROUND TO THE INVENTION

[0005] Epilepsy occurs in approximately 1% of the population worldwide, (Thurman et al., 2011) of which 70% are able to adequately control their symptoms with the available existing anti-epileptic drugs (AED). However, 30% of this patient group, (Eadie et al., 2012), are unable to obtain seizure freedom from the AED that are available and as such are termed as suffering from intractable or "treatment-resistant epilepsy" (IRE).

[0006] Intractable or treatment-resistant epilepsy was defined in 2009 by the International League Against Epilepsy (ILAE) as "failure of adequate trials of two tolerated and appropriately chosen and used AED schedules (whether as monotherapies or in combination) to achieve sustained seizure freedom" (Kwan et al., 2009).

[0007] Individuals who develop epilepsy during the first few years of life are often difficult to treat and as such are often termed treatment resistant. Children who undergo frequent seizures in childhood are often left with neurological damage which can cause cognitive, behavioral and motor delays.

[0008] Childhood epilepsy is a relatively common neurological disorder in children and young adults with a prevalence of approximately 700 per 100,000. This is twice the number of epileptic adults per population.

[0009] When a child or young adult presents with a seizure, investigations are normally undertaken in order to investigate the cause. Childhood epilepsy can be caused by many different syndromes and genetic mutations and as such diagnosis for these children may take some time.

[0010] The main symptom of epilepsy is repeated seizures. In order to determine the type of epilepsy or the epileptic syndrome that a patient is suffering from an investigation into the type of seizures that the patient is experiencing is undertaken. Clinical observations and electroencephalography (EEG) tests are conducted and the type(s) of seizures are classified according to the ILEA classification.

[0011] Generalized seizures, where the seizure arises within and rapidly engages bilaterally distributed networks, can be split into six subtypes: tonic-clonic (grand mal) seizures; absence (petit mal) seizures; clonic seizures; tonic seizures; atonic seizures and myoclonic seizures.

[0012] Focal (partial) seizures where the seizure originates within networks limited to only one hemisphere, are also split into sub-categories. Here the seizure is characterized according to one or more features of the seizure, including aura, motor, autonomic and awareness / responsiveness. Where a seizure begins as a localized seizure and rapidly evolves to be distributed within bilateral networks this seizure is known as a bilateral convulsive seizure, which is the proposed terminology to replace secondary generalized seizures (generalized seizures that have evolved from focal seizures and are no longer remain localized).

[0013] Focal seizures where the subject's awareness! responsiveness is altered are referred to as focal seizures with impairment and focal seizures where the awareness or responsiveness of the subject is not impaired are referred to as focal seizures without impairment.

[0014] Perisylvian fissure syndrome, also known as bilateral perisylvian polymicrogyria, is a rare neurological disorder that affects the cerebral cortex. The cerebral cortex of the brain normally consists of several deep folds and grooves. In perisylvian fissure syndrome, the grooves that develop during embryonic growth form improperly on both sides of the brain near the Sylvian fissure, resulting in an increased number of folds that are unusually small.

[0015] Symptoms typically become apparent at birth, infancy, or later during childhood, and may include partial paralysis of the face, tongue, jaw, and throat; problems with speech and swallowing; excessive drooling; and seizures. Mild to severe intellectual disability often is also present.

[0016] Most cases of perisylvian fissure syndrome occur sporadically in people with no family history. Rarely, more than one family member has perisylvian fissure syndrome, and the pattern of inheritance depends on the cause.

[0017] Diagnosis of perisylvian fissure syndrome is based on the symptoms present and a thorough neurological evaluation including various imaging techniques. Treatment aims to address individual symptoms present in each person.

[0018] The quality of life and life expectancy for people with perisylvian fissure syndrome are not well-described but may depend on severity of symptoms, whether complications develop, and whether other birth defects or an underlying syndrome are present.

[0019] Cannabidiol (CBD), a non-psychoactive derivative from the cannabis plant, has demonstrated anti-convulsant properties in several anecdotal reports, pre-clinical and clinical studies both in animal models and humans. Three randomized control trials showed efficacy of the purified pharmaceutical formulation of CBD in patients with Dravet and Lennox-Gastaut syndrome.

[0020] Based on these three trials, a botanically derived purified CBD preparation was approved by FDA in June 2018 for the treatment of seizures associated with Dravet and Lennox-Gastaut syndromes.

[0021] The applicant has found by way of an open label, expanded-access program that treatment with CBD resulted in a significant reduction in seizures in a patient with perisylvian fissure syndrome.

BRIEF SUMMARY OF THE DISCLOSURE

[0022] In accordance with a first aspect of the present invention there is provided a cannabidiol (CBD) preparation for use in the treatment of perisylvian fissure syndrome.

[0023] In a further embodiment, the seizures associated with perisylvian fissure syndrome are focal seizures with impairment.

[0024] In a further embodiment, the CBD preparation comprises greater than 95% (w/w) CBD and not more than 0.15% (w/w) tetrahydrocannabinol (THC).

[0025] Preferably the CBD preparation comprises greater than or equal to 98% (w/w) CBD and less than or equal to 2% (w/w) other cannabinoids, wherein the less than or equal to 2% (w/w) other cannabinoids comprise the cannabinoids tetrahydrocannabinol (THC); cannabidiolC1 (CBD-C1); cannabidivarin (CBDV); and cannabidiol-C4 (CBD-C4), and wherein the THC is present as a mixture of trans-THC and cis-THC.

[0026] Preferably the CBD preparation is used in combination with one or more concomitant anti-epileptic drugs (AED).

[0027] Preferably the one or more AED is taken from the group consisting of: clonazepam, diazepam, lacosamide and levefiracetam.

[0028] In one embodiment the CBD is present is isolated from cannabis plant material.

Preferably at least a portion of at least one of the cannabinoids present in the CBD preparation is isolated from cannabis plant material.

[0029] In a further embodiment the CBD is present as a synthetic preparation. Preferably at least a portion of at least one of the cannabinoids present in the CBD preparation is prepared synthetically.

[0030] Preferably the dose of CBD is greater than 5 mg/kg/day. More preferably the dose of CBD is 20 mg/kg/day. More preferably the dose of CBD is 25 mg/kg/day. More preferably the dose of CBD is 50 mg/kg/day.

[0031] In accordance with a second aspect of the present invention there is provided a method of treating seizures associated with perisylvian fissure syndrome comprising administering a cannabidiol (CBD) preparation to the subject in need thereof.

DEFINITIONS

[0032] Definitions of some of the terms used to describe the invention are detailed below: [0033] Over 100 different cannabinoids have been identified, see for example, Handbook of Cannabis, Roger Pertwee, Chapter 1, pages 3 to 15. These cannabinoids can be split into different groups as follows: Phytocannabinoids; Endocannabinoids and Synthetic cannabinoids (which may be novel cannabinoids or synthetically produced phytocannabinoids or endocannabinoids).

[0034] "Phytocannabinoids" are cannabinoids that originate from nature and can be found in the cannabis plant. The phytocannabinoids can be isolated from plants to produce a highly purified extract or can be reproduced synthetically.

[0035] "Highly purified cannabinoids" are defined as cannabinoids that have been extracted from the cannabis plant and purified to the extent that other cannabinoids and non-cannabinoid components that are co-extracted with the cannabinoids have been removed, such that the highly purified cannabinoid is greater than or equal to 95% (w/w) pure.

[0036] "Synthetic cannabinoids" are compounds that have a cannabinoid or cannabinoid-like structure and are manufactured using chemical means rather than by the plant.

[0037] Phytocannabinoids can be obtained as either the neutral (decarboxylated form) or the carboxylic acid form depending on the method used to extract the cannabinoids. For example, it is known that heating the carboxylic acid form will cause most of the carboxylic acid form to decarboxylate into the neutral form.

[0038] "Treatment-resistant epilepsy" (TRE) or "intractable epilepsy" is defined as per the I LAE guidance of 2009 as epilepsy that is not adequately controlled by trials of one or more AED.

[0039] "Focal Seizures" are defined as seizures which originate within networks limited to only one hemisphere. What happens during the seizure depends on where in the brain the seizure happens and what that part of the brain normally does.

[0040] "Focal seizure with impairment" has replaced the term "complex partial seizure".

These seizures usually start in a small area of the temporal lobe or frontal lobe of the brain and involve other areas of the brain within the same hemisphere that affect alertness and awareness. Most subjects experience automatisms during a focal seizure with impaired consciousness.

DETAILED DESCRIPTION

PREPARATION OF HIGHLY PURIFIED CBD EXTRACT

[0041] The following describes the production of the highly-purified (>95% w/w) cannabidiol extract which has a known and constant composition.

[0042] In summary the drug substance used is a liquid carbon dioxide extract of high-CBD containing chemotypes of Cannabis sativa L. which had been further purified by a solvent crystallization method to yield CBD. The crystallisation process specifically removes other cannabinoids and plant components to yield greater than or equal to 95% CBD. Although the CBD is highly purified because it is produced from a cannabis plant rather than synthetically there is a small number of other cannabinoids which are co-produced and co-extracted with the CBD. Details of these cannabinoids and the quantities in which they are present in the medication are as described in Table A below.

Table A: Composition of highly purified CBD extract Cannabinoid Concentration CBD > 95% w/w CBDA NMT 0.15% w/w CBDV NMT 1.0% w/w A9 THC NMT 0.15% w/w CBD-C4 NMT 0.5% w/w > -greater than NMT -not more than

PREPARATION OF BOTANICALLY DERIVED PURIFIED CBD

[0043] The following describes the production of the botanically derived purified CBD which comprises greater than or equal to 98% w/w CBD and less than or equal to other cannabinoids was used in the open label, expanded-access program described in Example 1 below.

[0044] In summary the drug substance used in the trials is a liquid carbon dioxide extract of high-CBD containing chemotypes of Cannabis sativa L. which had been further purified by a solvent crystallization method to yield CBD. The crystallisation process specifically removes other cannabinoids and plant components to yield greater than 95% CBD w/w, typically greater than 98% w/w.

[0045] The Cannabis sativa L. plants are grown, harvested, and processed to produce a botanical extract (intermediate) and then purified by crystallization to yield the CBD (botanically derived purified CBD).

[0046] The plant starting material is referred to as Botanical Raw Material (BRM); the botanical extract is the intermediate; and the active pharmaceutical ingredient (API) is CBD, the drug substance.

[0047] All parts of the process are controlled by specifications. The botanical raw material specification is described in Table B and the CBD API is described in Table C.

Table B: CBD botanical raw material specification

Test Method Specification

Identification: Visual TLC Complies -A HPLC/UV Corresponds to standard (for CBD & CBDA) Positive for CBDA

-B

-C

Assay: In-house (HPLC/UV) NLT 90% of assayed CBDA + CBD cannabinoids by peak area Loss on Drying Ph.Eur. NMT 15% Aflatoxin UKAS method NMT 4ppb Microbial: -TVC -Fungi -E.coli Ph.Eur. NMT107cfu/g NMT105cfu/g NMT102cfu/g Foreign Matter: Ph.Eur. NMT 2% Residual Herbicides and Pesticides Ph.Eur. Complies Table C: Specification of an exemplary botanically derived purified CBD preparation Test Test Method Limits Appearance Visual Off-white / pale yellow crystals Identification A HPLC-UV Retention time of major peak corresponds to certified CBD Reference Standard Identification B GC-FID/MS Retention time and mass spectrum of major peak corresponds to certified CBD Reference Standard Identification C FT-IR Conforms to reference spectrum for certified CBD Reference Standard Identification D Melting Point 65 -67°C Identification E Specific Optical Conforms with certified CBD Reference Rotation Standard; -110° to -140° (in 95% ethanol) Total Purity Calculation 98.0% Chromatographic Purity 1 HPLC-UV L 98.0% Chromatographic Purity 2 GC-FID/MS 98.0 % CBDA HPLC-UV N MT 0.15% w/w NMT 1.0% w/w NMT 0.1% w/w NMT 0.5% w/w

CBDV

THC

CBD-C4 Residual Solvents: GC NMT 0.5% w/w Alkane NMT 0.5% w/w Ethanol Residual Water Karl Fischer NMT 1.0% w/w [0048] The purity of the botanically derived purified CBD preparation was greater than or equal to 98%. The botanically derived purified CBD includes THC and other cannabinoids, e.g., CBDA, CBDV, CBD-C1, and CBD-C4.

[0049] Distinct chemotypes of the Cannabis sativa L. plant have been produced to maximize the output of the specific chemical constituents, the cannabinoids. Certain chemovars produce predominantly CBD. Only the (-)-trans isomer of CBD is believed to occur naturally. During purification, the stereochemistry of CBD is not affected.

Production of CBD botanical drug substance [0050] An overview of the steps to produce a botanical extract, the intermediate, are as follows: a) Growing b) Direct drying c) Decarboxylafion d) Extraction -using liquid CO2 e) Winterization using ethanol Filtration 9) Evaporation [0051] High CBD chemovars were grown, harvested, dried, baled and stored in a dry room until required. The botanical raw material (BRM) was finely chopped using an Apex mill fitted with a 1 mm screen. The milled BRM was stored in a freezer prior to extraction.

[0052] Decarboxylation of CBDA to CBD was carried out using heat. BRM was decarboxylated at 115°C for 60 minutes.

[0053] Extraction was performed using liquid CO2 to produce botanical drug substance (BDS), which was then crystalized to produce the test material. The crude CBD BDS was winterized to refine the extract under standard conditions (2 volumes of ethanol at -20°C for approximately 50 hours). The precipitated waxes were removed by filtration and the solvent was removed to yield the BDS.

Production of botanically derived purified CBD preparation [0054] The manufacturing steps to produce the botanically derived purified CBD preparation from BDS were as follows: a) Crystallization using 05-012 straight chain or branched alkane b) Filtration c) Vacuum drying [0055] The BDS produced using the methodology above was dispersed in C5-C12 straight chain or branched alkane. The mixture was manually agitated to break up any lumps and the sealed container then placed in a freezer for approximately 48 hours. The crystals were isolated via vacuum filtration, washed with aliquots of cold C5-C12 straight chain or branched alkane, and dried under a vacuum of <10mb at a temperature of 60°C until dry. The botanically derived purified CBD preparation was stored in a freezer at -20°C in a pharmaceutical grade stainless steel container, with FDA food grade approved silicone seal and clamps.

Physicochemical properties of the botanically derived purified CBD [0056] The botanically derived purified CBD used in the clinical trial described in the invention comprises greater than or equal to 98% (w/w) CBD and less than or equal to 2% (w/w) of other cannabinoids. The other cannabinoids present are THC at a concentration of less than or equal to 0.1% (w/w); CBD-C1 at a concentration of less than or equal to 0.15% (w/w); CBDV at a concentration of less than or equal to 0.8% (w/w); and CBD-C4 at a concentration of less than or equal to 0.4% (w/w).

[0057] The botanically derived purified CBD used additionally comprises a mixture of both trans-THC and cis-THC. It was found that the ratio of the trans-THC to cis-THC is altered and can be controlled by the processing and purification process, ranging from 3.3:1 (transTHC:cis-THC) in its unrefined decarboxylated state to 0.8:1 (trans-THC:cis-THC) when highly purified.

[0058] Furthermore, the cis-THC found in botanically derived purified CBD is present as a mixture of both the (+)-cis-THC and the (-)-cis-THC isoforms.

[0059] Clearly a CBD preparation could be produced synthetically by producing a composition with duplicate components.

[0060] Example 1 below describes the use of a botanically derived purified CBD in an open label, expanded-access program to investigate the clinical efficacy and safety of purified pharmaceutical cannabidiol formulation (CBD) in the treatment perisylvian fissure syndrome.

EXAMPLE 1: CLINICAL EFFICACY AND SAFETY OF PURIFIED PHARMACEUTICAL CANNABIDIOL (CBD) IN THE TREATMENT OF A PATIENT DIAGNOSED PERISYLVIAN FISSURE SYNDROME Study design [0061] The subject was required to be on one or more AEDs at stable doses for a minimum of two weeks prior to baseline and to have stable vagus nerve stimulation (VNS) settings and ketogenic diet ratios for a minimum of four weeks prior to baseline.

[0062] The patient was administered botanically derived purified CBD in a 100 mg/mL sesame oil-based solution at an initial dose of 5 milligrams per kilogram per day (mg/kg/day) in two divided doses. Dose was then increased weekly by 5mg/kg/day to a goal of 20 to 25 mg/kg/day.

[0063] A maximum dose of 50 mg/kg/day could be utilised should the patient require it to achieve seizure control; further weekly titration by 5mg/kg/day is additionally required for these higher doses.

[0064] There was one patient in this study, and they received CBD for 96 weeks. Modifications were made to concomitant AEDs as per clinical indication.

[0065] Seizure frequency, intensity, and duration were recorded by caregivers in a diary during a baseline period of at least 28 days. Changes in seizure frequency relative to baseline were calculated after at least 2 weeks and at defined timepoints of treatment.

Statistical Methods: [0066] Patients may be defined as responders if they had more than 50% reduction in seizure frequency compared to baseline. The percent change in seizure frequency was calculated as follows: % change= ((weekly seizure frequency time interval)-(weekly seizure frequency Baseline)) x100 seizure (weekly seizure frequency Baseline) frequency [0067] The percent change of seizure frequency may be calculated for any time interval where seizure number has been recorded. For the purpose of this example the percent change of seizure frequency for the end of the treatment period was calculated as follows: % reduction = ((weekly seizure frequency Baseline) -(weekly seizure frequency End)) x100 seizure frequency (weekly seizure frequency Baseline) Results

Patient description

[0068] There was one patient enrolled in the open label, expanded-access program which was diagnosed with perisylvian fissure syndrome. This patient experienced focal seizures with impairment and was taking several concomitant AEDs.

[0069] The age of the patient was 19 years old, and she was female as detailed in Table 1 below.

Table 1: Patient demographics, seizure type and concomitant medication Patient Age Sex Seizure types Concomitant AEDs Number (years) 1 19.96 F Focal seizures with CLZ, DPM, LCM, LEV impairment CLZ = clonazepam, DPM = diazepam, LCM = lacosamide, LEV = levetiracetam Study medication and concomitant medications [0070] The patient on the study were titrated up to a dose of 30mg/kg/day of CBD. The patient was taking four concomitant AEDs at the time of starting CBD.

Clinical changes [0071] Table 2 illustrates the seizure frequency for the patient as well as the dose of CBD given.

Table 2: Seizure frequency data for Patient 1 Patient 1 Time Seizure Type Dose CBD (mg/kg/day) Focal with impairment Baseline 82.0 - 2 weeks 40.0 5.0 4 weeks 24.0 10.0 8 weeks 12.0 15.0 12 weeks 22.0 25.0 16 weeks 10.0 20.0 24 weeks 6.6 20.0 48 weeks 12.0 30.0 weeks 10.0 30.0 72 weeks 12.0 30.0 84 weeks 20.0 30.0 96 weeks 18.0 0 [0072] Patient 1 was treated for 84 weeks and experienced a 78% reduction in focal seizures with impairment over the treatment period.

[0073] Overall, CBD was particularly effective in reducing the frequency focal seizures with impairment.

Conclusions

[0074] These data indicate that CBD was able to significantly reduce the number of seizures associated with perisylvian fissure syndrome. Clearly the treatment is of significant benefit in this rare syndrome given the high response rate experienced this patient. In conclusion, this study signifies the use of CBD for treatment of seizures associated with perisylvian fissure syndrome. Seizure types include focal seizures with impairment which were decreased by significant rates up to 78%.

Claims (15)

1. CLAIMS 1. 3. 4. 5. 6. 7. 8.A cannabidiol (CBD) preparation for use in the treatment of seizures associated with perisylvian fissure syndrome.
2. A CBD preparation for use according to claim 1, wherein the seizures associated with perisylvian fissure syndrome are focal seizures with impairment.
3. A CBD preparation for use according to any of the preceding claims, wherein the CBD preparation comprises greater than 95% (w/w) CBD and not more than 0.15% (w/w) tetrahydrocannabinol (THC).
4. A CBD preparation for use according to any of the preceding claims, wherein the CBD preparation comprises greater than or equal to 98% (w/w) CBD and less than or equal to 2% (w/w) other cannabinoids, wherein the less than or equal to 2% (w/w) other cannabinoids comprise the cannabinoids tetrahydrocannabinol (THC); cannabidiol-C1 (CBD-C1); cannabidivarin (CBDV); and cannabidiol-C4 (CBD-C4), and wherein the THC is present as a mixture of trans-THC and cis-THC.
5. A CBD preparation to any of the preceding claims, wherein the CBD preparation is used in combination with one or more concomitant anti-epileptic drugs (AED).
6. A CBD preparation for use according to claim 5, wherein the one or more AED is taken from the group consisting of: clonazepam, diazepam, lacosamide and levefiracetam.
7. A CBD preparation for use according to any of the preceding claims, wherein the CBD is present is isolated from cannabis plant material.
8. A CBD preparation for use according to any of the preceding claims, wherein at least a portion of at least one of the cannabinoids present in the CBD preparation is isolated from cannabis plant material.
9. A CBD preparation for use according to claims 1 to 6, wherein the CBD is present as a synthetic preparation.
10. 10. A CBD preparation for use according to claim 9, wherein at least a portion of at least one of the cannabinoids present in the CBD preparation is prepared synthetically.
11. 11. A CBD preparation for use according to any of the preceding claims, wherein the dose of CBD is greater than 5 mg/kg/day.
12. 12. A CBD preparation for use according to any of the preceding claims, wherein the dose of CBD is 20 mg/kg/day.
13. 13. A CBD preparation for use according to any of the preceding claims, wherein the dose of CBD is 25 mg/kg/day.
14. 14. A CBD preparation for use according to any of the preceding claims, wherein the dose of CBD is 50 mg/kg/day.
15. 15. A method of treating seizures associated with perisylvian fissure syndrome comprising administering a cannabidiol (CBD) preparation to the subject in need thereof.