GB2570404A - Cell contamination assay - Google Patents
Cell contamination assay Download PDFInfo
- Publication number
- GB2570404A GB2570404A GB1900893.7A GB201900893A GB2570404A GB 2570404 A GB2570404 A GB 2570404A GB 201900893 A GB201900893 A GB 201900893A GB 2570404 A GB2570404 A GB 2570404A
- Authority
- GB
- United Kingdom
- Prior art keywords
- hsa
- mir
- psc
- cells
- contaminants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000011109 contamination Methods 0.000 title claims abstract description 99
- 238000003556 assay Methods 0.000 title description 41
- 210000004027 cell Anatomy 0.000 claims abstract description 422
- 210000001778 pluripotent stem cell Anatomy 0.000 claims abstract description 311
- 238000000034 method Methods 0.000 claims abstract description 206
- 239000000356 contaminant Substances 0.000 claims abstract description 143
- 108091027963 non-coding RNA Proteins 0.000 claims abstract description 115
- 102000042567 non-coding RNA Human genes 0.000 claims abstract description 115
- 238000002659 cell therapy Methods 0.000 claims abstract description 68
- 239000002679 microRNA Substances 0.000 claims description 210
- 108091070501 miRNA Proteins 0.000 claims description 122
- 230000014509 gene expression Effects 0.000 claims description 73
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims description 60
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 48
- 238000011304 droplet digital PCR Methods 0.000 claims description 47
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 34
- 239000013641 positive control Substances 0.000 claims description 30
- 238000001514 detection method Methods 0.000 claims description 28
- 108091065459 Homo sapiens miR-302a stem-loop Proteins 0.000 claims description 27
- 108091067250 Homo sapiens miR-302b stem-loop Proteins 0.000 claims description 27
- 108091067264 Homo sapiens miR-302c stem-loop Proteins 0.000 claims description 27
- 108091067255 Homo sapiens miR-302d stem-loop Proteins 0.000 claims description 23
- 239000002299 complementary DNA Substances 0.000 claims description 22
- 102000040650 (ribonucleotides)n+m Human genes 0.000 claims description 13
- 108091067254 Homo sapiens miR-367 stem-loop Proteins 0.000 claims description 13
- 108091067269 Homo sapiens miR-371a stem-loop Proteins 0.000 claims description 12
- 108091067564 Homo sapiens miR-373 stem-loop Proteins 0.000 claims description 12
- 239000002773 nucleotide Substances 0.000 claims description 12
- 125000003729 nucleotide group Chemical group 0.000 claims description 12
- 206010043276 Teratoma Diseases 0.000 claims description 11
- 108091067570 Homo sapiens miR-372 stem-loop Proteins 0.000 claims description 9
- 108091064467 Homo sapiens miR-520c stem-loop Proteins 0.000 claims description 9
- 230000004069 differentiation Effects 0.000 claims description 9
- 108091092274 Homo sapiens miR-512-1 stem-loop Proteins 0.000 claims description 8
- 108091092275 Homo sapiens miR-512-2 stem-loop Proteins 0.000 claims description 8
- 108020004635 Complementary DNA Proteins 0.000 claims description 7
- 108091067482 Homo sapiens miR-205 stem-loop Proteins 0.000 claims description 7
- 239000000090 biomarker Substances 0.000 claims description 6
- 230000000295 complement effect Effects 0.000 claims description 6
- 238000007847 digital PCR Methods 0.000 claims description 5
- 238000009396 hybridization Methods 0.000 claims description 5
- 230000001939 inductive effect Effects 0.000 claims description 5
- 102000040430 polynucleotide Human genes 0.000 claims description 5
- 108091033319 polynucleotide Proteins 0.000 claims description 5
- 239000002157 polynucleotide Substances 0.000 claims description 5
- 108091068928 Homo sapiens miR-107 stem-loop Proteins 0.000 claims description 4
- 108091069019 Homo sapiens miR-124-1 stem-loop Proteins 0.000 claims description 4
- 108091069008 Homo sapiens miR-124-2 stem-loop Proteins 0.000 claims description 4
- 108091069007 Homo sapiens miR-124-3 stem-loop Proteins 0.000 claims description 4
- 108091069022 Homo sapiens miR-130a stem-loop Proteins 0.000 claims description 4
- 108091060456 Homo sapiens miR-1323 stem-loop Proteins 0.000 claims description 4
- 108091069088 Homo sapiens miR-150 stem-loop Proteins 0.000 claims description 4
- 108091067605 Homo sapiens miR-183 stem-loop Proteins 0.000 claims description 4
- 108091072943 Homo sapiens miR-3141 stem-loop Proteins 0.000 claims description 4
- 108091072772 Homo sapiens miR-3149 stem-loop Proteins 0.000 claims description 4
- 108091065451 Homo sapiens miR-34b stem-loop Proteins 0.000 claims description 4
- 108091067286 Homo sapiens miR-363 stem-loop Proteins 0.000 claims description 4
- 108091056625 Homo sapiens miR-3692 stem-loop Proteins 0.000 claims description 4
- 108091035126 Homo sapiens miR-4327 stem-loop Proteins 0.000 claims description 4
- 108091092282 Homo sapiens miR-498 stem-loop Proteins 0.000 claims description 4
- 108091092284 Homo sapiens miR-515-1 stem-loop Proteins 0.000 claims description 4
- 108091092278 Homo sapiens miR-515-2 stem-loop Proteins 0.000 claims description 4
- 108091064421 Homo sapiens miR-516b-1 stem-loop Proteins 0.000 claims description 4
- 108091064453 Homo sapiens miR-516b-2 stem-loop Proteins 0.000 claims description 4
- 108091064445 Homo sapiens miR-517a stem-loop Proteins 0.000 claims description 4
- 108091064447 Homo sapiens miR-517b stem-loop Proteins 0.000 claims description 4
- 108091064470 Homo sapiens miR-518b stem-loop Proteins 0.000 claims description 4
- 108091064474 Homo sapiens miR-519b stem-loop Proteins 0.000 claims description 4
- 108091064454 Homo sapiens miR-519d stem-loop Proteins 0.000 claims description 4
- 108091064471 Homo sapiens miR-525 stem-loop Proteins 0.000 claims description 4
- 108091061636 Homo sapiens miR-630 stem-loop Proteins 0.000 claims description 4
- 108091061569 Homo sapiens miR-663a stem-loop Proteins 0.000 claims description 4
- 108091070376 Homo sapiens miR-96 stem-loop Proteins 0.000 claims description 4
- 238000003757 reverse transcription PCR Methods 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 4
- 108091070383 Homo sapiens miR-32 stem-loop Proteins 0.000 claims description 3
- 108091061631 Homo sapiens miR-629 stem-loop Proteins 0.000 claims description 3
- 238000007826 nucleic acid assay Methods 0.000 claims description 3
- 239000000523 sample Substances 0.000 description 97
- 108700011259 MicroRNAs Proteins 0.000 description 91
- 230000035945 sensitivity Effects 0.000 description 28
- 238000012341 Quantitative reverse-transcriptase PCR Methods 0.000 description 21
- 238000002493 microarray Methods 0.000 description 21
- 239000013068 control sample Substances 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 16
- 238000011948 assay development Methods 0.000 description 16
- 239000000047 product Substances 0.000 description 15
- 238000005259 measurement Methods 0.000 description 12
- 210000000130 stem cell Anatomy 0.000 description 10
- 210000001671 embryonic stem cell Anatomy 0.000 description 7
- 238000010206 sensitivity analysis Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 238000010195 expression analysis Methods 0.000 description 5
- -1 hsa-miR-3733p Proteins 0.000 description 5
- 238000010208 microarray analysis Methods 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000003908 quality control method Methods 0.000 description 5
- 239000004055 small Interfering RNA Substances 0.000 description 5
- 238000002560 therapeutic procedure Methods 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000001185 bone marrow Anatomy 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000012207 quantitative assay Methods 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 102100034343 Integrase Human genes 0.000 description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 3
- 238000011529 RT qPCR Methods 0.000 description 3
- 102000042773 Small Nucleolar RNA Human genes 0.000 description 3
- 108020003224 Small Nucleolar RNA Proteins 0.000 description 3
- 210000001744 T-lymphocyte Anatomy 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 238000010804 cDNA synthesis Methods 0.000 description 3
- 210000004700 fetal blood Anatomy 0.000 description 3
- 210000005260 human cell Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 108091007413 Extracellular RNA Proteins 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 108091030146 MiRBase Proteins 0.000 description 2
- 238000002123 RNA extraction Methods 0.000 description 2
- 239000013614 RNA sample Substances 0.000 description 2
- 102000039471 Small Nuclear RNA Human genes 0.000 description 2
- 108091027967 Small hairpin RNA Proteins 0.000 description 2
- 108020004459 Small interfering RNA Proteins 0.000 description 2
- 210000001789 adipocyte Anatomy 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 210000002798 bone marrow cell Anatomy 0.000 description 2
- 210000004413 cardiac myocyte Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 210000001612 chondrocyte Anatomy 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 210000003074 dental pulp Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 230000003291 dopaminomimetic effect Effects 0.000 description 2
- 210000004696 endometrium Anatomy 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 239000008274 jelly Substances 0.000 description 2
- 238000003253 miRNA assay Methods 0.000 description 2
- 238000010606 normalization Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 210000002027 skeletal muscle Anatomy 0.000 description 2
- 108091029842 small nuclear ribonucleic acid Proteins 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 210000003954 umbilical cord Anatomy 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000009329 Graft vs Host Disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 108091007412 Piwi-interacting RNA Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 206010067584 Type 1 diabetes mellitus Diseases 0.000 description 1
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 238000004422 calculation algorithm Methods 0.000 description 1
- 229940030602 cardiac therapy drug Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 238000013481 data capture Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 208000024908 graft versus host disease Diseases 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000002064 heart cell Anatomy 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
- 238000005462 in vivo assay Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 208000017169 kidney disease Diseases 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000003061 neural cell Anatomy 0.000 description 1
- 210000001178 neural stem cell Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 210000004409 osteocyte Anatomy 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012175 pyrosequencing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000008672 reprogramming Effects 0.000 description 1
- 230000002207 retinal effect Effects 0.000 description 1
- 230000000250 revascularization Effects 0.000 description 1
- 238000002976 reverse transcriptase assay Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 210000001988 somatic stem cell Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C12N2310/141—MicroRNAs, miRNAs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/04—Screening or testing on artificial tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/207—Modifications characterised by siRNA, miRNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2539/00—Reactions characterised by analysis of gene expression or genome comparison
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Rheumatology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Hematology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1801035.5A GB201801035D0 (en) | 2018-01-22 | 2018-01-22 | Cell Contamination Assay |
| GBGB1809337.7A GB201809337D0 (en) | 2018-06-06 | 2018-06-06 | Cell contamination assay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB201900893D0 GB201900893D0 (en) | 2019-03-13 |
| GB2570404A true GB2570404A (en) | 2019-07-24 |
Family
ID=65409042
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1900893.7A Withdrawn GB2570404A (en) | 2018-01-22 | 2019-01-22 | Cell contamination assay |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20210139984A1 (https=) |
| EP (1) | EP3743531A1 (https=) |
| JP (1) | JP2021511076A (https=) |
| CA (1) | CA3127507A1 (https=) |
| GB (1) | GB2570404A (https=) |
| WO (1) | WO2019141878A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021175768A1 (en) * | 2020-03-02 | 2021-09-10 | Novo Nordisk A/S | Use of pluripotent markers to detect contaminating residual undifferentiated pluripotent stem cells |
| WO2021182477A1 (en) * | 2020-03-09 | 2021-09-16 | Fujifilm Corporation | Markers specific for pluripotent stem cells, and methods of using the same |
| CA3211361A1 (en) * | 2021-03-08 | 2022-09-15 | FUJIFILM Cellular Dynamics, Inc. | Markers specific for pluripotent stem cells, and methods of using the same |
| CN113355433B (zh) * | 2021-06-02 | 2022-07-19 | 呈诺再生医学科技(珠海横琴新区)有限公司 | 一种基于单细胞测序数据分析的iPSC残留检测方法 |
| WO2024222806A1 (zh) * | 2023-04-26 | 2024-10-31 | 浙江霍健德生物科技有限公司 | 用于iPSC残留检测的生物标志物及其筛选方法和用途 |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005056797A1 (en) * | 2003-12-15 | 2005-06-23 | Kye-Seong Kim | Novel mirna molecules isolated from human embryonic stem cell |
| WO2010115050A2 (en) * | 2009-04-01 | 2010-10-07 | The Regents Of The University Of California | Embryonic stem cell specific micrornas promote induced pluripotency |
| US20110171183A1 (en) * | 2008-09-18 | 2011-07-14 | Agency For Science, Technology And Research | Markers of Induced Pluripotent Stem Cells |
| WO2011087154A1 (en) * | 2010-01-15 | 2011-07-21 | Kyoto University | Method for screening induced pluripotent stem cells |
| WO2012025709A1 (en) * | 2010-08-23 | 2012-03-01 | Sistemic Uk | Cell characterisation |
| WO2018090084A1 (en) * | 2016-11-16 | 2018-05-24 | Cynata Therapeutics Limited | Pluripotent stem cell assay |
| WO2018135288A1 (ja) * | 2017-01-19 | 2018-07-26 | シスメックス株式会社 | 細胞の分化状態を評価する方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3049067A4 (en) * | 2013-09-24 | 2017-05-03 | The Regents of the University of California | Encapsulated sensors and sensing systems for bioassays and diagnostics and methods for making and using them |
| US10604770B2 (en) * | 2014-07-16 | 2020-03-31 | Kyoto University | Method for extracting differentiated cells |
| WO2016150475A1 (en) * | 2015-03-22 | 2016-09-29 | Universite De Liege | Circulating micrornas for the diagnosis of breast cancer |
| EP3181698A1 (en) * | 2015-12-16 | 2017-06-21 | European Molecular Biology Laboratory (EMBL) | Microrna mir-142 as stem cell marker |
-
2019
- 2019-01-22 US US16/963,325 patent/US20210139984A1/en active Pending
- 2019-01-22 WO PCT/EP2019/051530 patent/WO2019141878A1/en not_active Ceased
- 2019-01-22 GB GB1900893.7A patent/GB2570404A/en not_active Withdrawn
- 2019-01-22 EP EP19704735.0A patent/EP3743531A1/en active Pending
- 2019-01-22 JP JP2020560565A patent/JP2021511076A/ja active Pending
- 2019-01-22 CA CA3127507A patent/CA3127507A1/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005056797A1 (en) * | 2003-12-15 | 2005-06-23 | Kye-Seong Kim | Novel mirna molecules isolated from human embryonic stem cell |
| US20110171183A1 (en) * | 2008-09-18 | 2011-07-14 | Agency For Science, Technology And Research | Markers of Induced Pluripotent Stem Cells |
| WO2010115050A2 (en) * | 2009-04-01 | 2010-10-07 | The Regents Of The University Of California | Embryonic stem cell specific micrornas promote induced pluripotency |
| WO2011087154A1 (en) * | 2010-01-15 | 2011-07-21 | Kyoto University | Method for screening induced pluripotent stem cells |
| WO2012025709A1 (en) * | 2010-08-23 | 2012-03-01 | Sistemic Uk | Cell characterisation |
| WO2018090084A1 (en) * | 2016-11-16 | 2018-05-24 | Cynata Therapeutics Limited | Pluripotent stem cell assay |
| WO2018135288A1 (ja) * | 2017-01-19 | 2018-07-26 | シスメックス株式会社 | 細胞の分化状態を評価する方法 |
Non-Patent Citations (1)
| Title |
|---|
| Sci. Reports, Vol.6, 2016, Parr, C. J. C. et al., "MicroRNA-302 switch to identify and eliminate...", Article No.: 32532 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019141878A1 (en) | 2019-07-25 |
| JP2021511076A (ja) | 2021-05-06 |
| EP3743531A1 (en) | 2020-12-02 |
| GB201900893D0 (en) | 2019-03-13 |
| CA3127507A1 (en) | 2019-07-25 |
| US20210139984A1 (en) | 2021-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Courts et al. | Specific micro‐RNA signatures for the detection of saliva and blood in forensic body‐fluid identification | |
| Siddika et al. | Bringing MicroRNAs to light: methods for MicroRNA quantification and visualization in live cells | |
| Precazzini et al. | Measurements methods for the development of microRNA-based tests for cancer diagnosis | |
| US20210139984A1 (en) | Cell contamination assay | |
| Sebastiani et al. | Circulating microRNAs and diabetes mellitus: a novel tool for disease prediction, diagnosis, and staging? | |
| Schoolmeesters et al. | Functional profiling reveals critical role for miRNA in differentiation of human mesenchymal stem cells | |
| Guerau-de-Arellano et al. | miRNA profiling for biomarker discovery in multiple sclerosis: from microarray to deep sequencing | |
| Chen et al. | A combination of Let-7d, Let-7g and Let-7i serves as a stable reference for normalization of serum microRNAs | |
| Schamberger et al. | 3′ IsomiR species and DNA contamination influence reliable quantification of microRNAs by stem-loop quantitative PCR | |
| Wang et al. | Comparing the MicroRNA spectrum between serum and plasma | |
| Rekker et al. | Circulating microRNA Profile throughout the menstrual cycle | |
| Ioannidis et al. | Circulating microRNA profiles during the bovine oestrous cycle | |
| Milagro et al. | High-throughput sequencing of microRNAs in peripheral blood mononuclear cells: identification of potential weight loss biomarkers | |
| US10011873B2 (en) | Diagnostic miRNA markers for Parkinson disease | |
| US20120322680A1 (en) | Use of mirnas as biomarkers in glioma diagnosis | |
| US9169520B2 (en) | Cancer marker, method for evaluation of cancer by using the cancer marker, and evaluation reagent | |
| Wang et al. | Genome-wide maps of polyadenylation reveal dynamic mRNA 3′-end formation in mammalian cell lineages | |
| US8936909B2 (en) | Method for determining the origin of a sample | |
| Khan et al. | MicroRNAs and transplantation | |
| Castel et al. | Small-RNA sequencing identifies dynamic microRNA deregulation during skeletal muscle lineage progression | |
| EP4060039A1 (en) | Sensitive detection method for undifferentiated marker genes | |
| KR20140006775A (ko) | 세포 특성화 | |
| Han et al. | High-throughput sequencing reveals circulating miRNAs as potential biomarkers for measuring puberty onset in chicken (Gallus gallus) | |
| CN102443638B (zh) | 一种用于血清/血浆miRNA检测的内参及其应用 | |
| US20180251836A1 (en) | Novel mirna biomarkers and use thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |