GB2552724B - Purification of Annexin A5 by anion exchange and affinity chromatography - Google Patents

Purification of Annexin A5 by anion exchange and affinity chromatography Download PDF

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Publication number
GB2552724B
GB2552724B GB1700973.9A GB201700973A GB2552724B GB 2552724 B GB2552724 B GB 2552724B GB 201700973 A GB201700973 A GB 201700973A GB 2552724 B GB2552724 B GB 2552724B
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GB
United Kingdom
Prior art keywords
annexin
purification
anion exchange
affinity chromatography
affinity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1700973.9A
Other versions
GB2552724A (en
GB201700973D0 (en
Inventor
Moks Toomas
Christoph Reich Jan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Annexin Pharmaceuticals AB
Original Assignee
Annexin Pharmaceuticals AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Annexin Pharmaceuticals AB filed Critical Annexin Pharmaceuticals AB
Priority to GB1700973.9A priority Critical patent/GB2552724B/en
Priority claimed from GB1516516.0A external-priority patent/GB2542391A/en
Publication of GB201700973D0 publication Critical patent/GB201700973D0/en
Publication of GB2552724A publication Critical patent/GB2552724A/en
Application granted granted Critical
Publication of GB2552724B publication Critical patent/GB2552724B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
GB1700973.9A 2015-09-17 2015-09-17 Purification of Annexin A5 by anion exchange and affinity chromatography Active GB2552724B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1700973.9A GB2552724B (en) 2015-09-17 2015-09-17 Purification of Annexin A5 by anion exchange and affinity chromatography

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1700973.9A GB2552724B (en) 2015-09-17 2015-09-17 Purification of Annexin A5 by anion exchange and affinity chromatography
GB1516516.0A GB2542391A (en) 2015-09-17 2015-09-17 Process of manufacture

Publications (3)

Publication Number Publication Date
GB201700973D0 GB201700973D0 (en) 2017-03-08
GB2552724A GB2552724A (en) 2018-02-07
GB2552724B true GB2552724B (en) 2020-05-06

Family

ID=58462943

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1700973.9A Active GB2552724B (en) 2015-09-17 2015-09-17 Purification of Annexin A5 by anion exchange and affinity chromatography

Country Status (1)

Country Link
GB (1) GB2552724B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109794414B (en) * 2019-02-13 2020-08-25 淮阴工学院 Screening method of polydisperse polymer microspheres

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005099744A1 (en) * 2004-04-15 2005-10-27 Athera Biotechnologies Ab Annexin v for preventing atherothrombois and plaque rupture
WO2010140886A1 (en) * 2009-06-03 2010-12-09 Mosamedix B.V. Method for enhancing phagocytosis of phosphatidylserine-exposing cells
WO2011135071A1 (en) * 2010-04-29 2011-11-03 Baxter International Inc. Purification method for divalent cation binding proteins on anion exchange resin

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005099744A1 (en) * 2004-04-15 2005-10-27 Athera Biotechnologies Ab Annexin v for preventing atherothrombois and plaque rupture
WO2010140886A1 (en) * 2009-06-03 2010-12-09 Mosamedix B.V. Method for enhancing phagocytosis of phosphatidylserine-exposing cells
WO2011135071A1 (en) * 2010-04-29 2011-11-03 Baxter International Inc. Purification method for divalent cation binding proteins on anion exchange resin

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BMC Biotechnology, Vol 14, 2014, LA Marder et al, "Production of recombinant human annexin V by fed-batch cultivation", 33 *
J Biotechnology, Vol 36, 1994, TR Hawthorne et al, "Isolation and characterization of recombinant annexin V expressed in Saccharomyces cerevisiae", 129-143 *
Preparative biochemistry & biotechnology, Vol 30, 2000, LN Zhang et al, "Expression and purification of recombinant human annexin V in Escherichia coli", 305-312 *

Also Published As

Publication number Publication date
GB2552724A (en) 2018-02-07
GB201700973D0 (en) 2017-03-08

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