GB2544891B - Transient transfection method for retroviral production - Google Patents

Transient transfection method for retroviral production

Info

Publication number
GB2544891B
GB2544891B GB1619642.0A GB201619642A GB2544891B GB 2544891 B GB2544891 B GB 2544891B GB 201619642 A GB201619642 A GB 201619642A GB 2544891 B GB2544891 B GB 2544891B
Authority
GB
United Kingdom
Prior art keywords
transient transfection
transfection method
retroviral production
retroviral
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB1619642.0A
Other versions
GB201619642D0 (en
GB2544891A (en
Inventor
Johnson Sabine
Pallant Celeste
Vamva Eirini
Vink Conrad
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlaxoSmithKline Intellectual Property Development Ltd
Original Assignee
GlaxoSmithKline Intellectual Property Development Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1520764.0A external-priority patent/GB201520764D0/en
Application filed by GlaxoSmithKline Intellectual Property Development Ltd filed Critical GlaxoSmithKline Intellectual Property Development Ltd
Publication of GB201619642D0 publication Critical patent/GB201619642D0/en
Publication of GB2544891A publication Critical patent/GB2544891A/en
Application granted granted Critical
Publication of GB2544891B publication Critical patent/GB2544891B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/00051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
GB1619642.0A 2015-11-24 2016-11-21 Transient transfection method for retroviral production Active GB2544891B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1520764.0A GB201520764D0 (en) 2015-11-24 2015-11-24 Transient transfection method for retroviral production
GBGB1609354.4A GB201609354D0 (en) 2016-05-26 2016-05-26 Transient transfection method for retroviral production

Publications (3)

Publication Number Publication Date
GB201619642D0 GB201619642D0 (en) 2017-01-04
GB2544891A GB2544891A (en) 2017-05-31
GB2544891B true GB2544891B (en) 2017-11-15

Family

ID=56410642

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB1609354.4A Ceased GB201609354D0 (en) 2015-11-24 2016-05-26 Transient transfection method for retroviral production
GB1619642.0A Active GB2544891B (en) 2015-11-24 2016-11-21 Transient transfection method for retroviral production

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB1609354.4A Ceased GB201609354D0 (en) 2015-11-24 2016-05-26 Transient transfection method for retroviral production

Country Status (1)

Country Link
GB (2) GB201609354D0 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043842A1 (en) * 1998-02-26 1999-09-02 Neurovir, Inc. Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant virus
WO2002053576A1 (en) * 2001-01-05 2002-07-11 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic dna inserts
WO2003087330A2 (en) * 2002-04-10 2003-10-23 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Vac-bac shuttle vector system
EP1743942A1 (en) * 2004-05-06 2007-01-17 The Research Foundation for Microbial Diseases of Osaka University Recombinant virus vector for gene introduction in lymphocyte
WO2008099148A1 (en) * 2007-02-12 2008-08-21 Ark Therapeutics Ltd. Production of lentiviral vectors
WO2009146150A2 (en) * 2008-04-04 2009-12-03 University Of Miami Viral recombineering and uses thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999043842A1 (en) * 1998-02-26 1999-09-02 Neurovir, Inc. Artificial chromosome constructs containing nucleic acid sequences capable of directing the formation of a recombinant virus
WO2002053576A1 (en) * 2001-01-05 2002-07-11 The General Hospital Corporation Viral delivery system for infectious transfer of large genomic dna inserts
WO2003087330A2 (en) * 2002-04-10 2003-10-23 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Vac-bac shuttle vector system
EP1743942A1 (en) * 2004-05-06 2007-01-17 The Research Foundation for Microbial Diseases of Osaka University Recombinant virus vector for gene introduction in lymphocyte
WO2008099148A1 (en) * 2007-02-12 2008-08-21 Ark Therapeutics Ltd. Production of lentiviral vectors
WO2009146150A2 (en) * 2008-04-04 2009-12-03 University Of Miami Viral recombineering and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Gene Therapy, Vol 15, 2008, HP Lesch et al, "Generation of lentivirus vectors using recombinant baculoviruses", 1280-1286 *

Also Published As

Publication number Publication date
GB201619642D0 (en) 2017-01-04
GB201609354D0 (en) 2016-07-13
GB2544891A (en) 2017-05-31

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