GB2544537A - Apparatus and method to determine physiological effects of cryogen on organisms for controlling freezers - Google Patents
Apparatus and method to determine physiological effects of cryogen on organisms for controlling freezers Download PDFInfo
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- GB2544537A GB2544537A GB1520478.7A GB201520478A GB2544537A GB 2544537 A GB2544537 A GB 2544537A GB 201520478 A GB201520478 A GB 201520478A GB 2544537 A GB2544537 A GB 2544537A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/36—Freezing; Subsequent thawing; Cooling
- A23L3/37—Freezing; Subsequent thawing; Cooling with addition of or treatment with chemicals
- A23L3/375—Freezing; Subsequent thawing; Cooling with addition of or treatment with chemicals with direct contact between the food and the chemical, e.g. liquid nitrogen, at cryogenic temperature
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/06—Freezing; Subsequent thawing; Cooling
- A23B4/08—Freezing; Subsequent thawing; Cooling with addition of chemicals or treatment with chemicals before or during cooling, e.g. in the form of an ice coating or frozen block
- A23B4/09—Freezing; Subsequent thawing; Cooling with addition of chemicals or treatment with chemicals before or during cooling, e.g. in the form of an ice coating or frozen block with direct contact between the food and the chemical, e.g. liquid N2, at cryogenic temperature
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/26—Accessories or devices or components used for biocidal treatment
- A61L2/28—Devices for testing the effectiveness or completeness of sterilisation, e.g. indicators which change colour
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D3/00—Devices using other cold materials; Devices using cold-storage bodies
- F25D3/10—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
- F25D3/11—Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air with conveyors carrying articles to be cooled through the cooling space
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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- General Chemical & Material Sciences (AREA)
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- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Food Science & Technology (AREA)
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- Biochemistry (AREA)
- Thermal Sciences (AREA)
- Mechanical Engineering (AREA)
- Combustion & Propulsion (AREA)
- Analytical Chemistry (AREA)
- Nutrition Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Sustainable Development (AREA)
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- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
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Abstract
An apparatus to determine physiological effects of heat flux with a cryogenic substance applied to an organism, includes a heat flux sensor 12, a cryogen delivery apparatus (e.g. a spray nozzle 18) spaced apart a distance D1 from the heat flux sensor 12; and an organism culture 28 supported on the heat flux sensor 12 in the path of delivery of the cryogenic substance from the cryogen delivery apparatus 18. A related method is also provided to determine physiological effects of cryogen on an organism, and includes testing a live organism culture for heat flux with a cryogenic substance; and establishing an amount of heat transfer required at the organism for achieving maximum destruction of said organism. The cryogenic substance may be liquid nitrogen, liquid oxygen or liquid argon, and the organism culture may comprise Campylobacter. The effects of the treatment on the micro-organisms may be determined by measuring the leakage of ATP from the cells.
Description
SPECIFICATION
APPARATUS AND METHOD TO DETERMINE PHYSIOLOGICAL EFFECTS OF CRYOGEN ON ORGANISMS FOR CONTROLLING FREEZERS
BACKGROUND OF THE INVENTION
[0001] The present embodiments relate to apparatus and methods using cooling to destroy organisms on products, and in particular to use cryogenics to destroy Campylobacter on food products.
[0002] Every product and every organism has a known rate of cooling and therefore, each product and organism can be preserved or destroyed at an optimum rate of such cooling.
[0003] In a freezer tunnel for food products for example, process control of heat flux occurring in such tunnel is critical to the operation of same for the most effective and efficient chilling or freezing of the food product. If an organism, such as for example Campylobacter, on the food product is to be destroyed it is necessary to know the effect of a cryogen, such as for example a liquid nitrogen (LIN) spray, on the Campylobacter in order to control the freezer tunnel operation for using that type of spray. Therefore, by understanding the effect of, for example, a LIN spray on the organism, one would be able to establish whether a particular LIN spray will be able to effectively destroy the organism cell, the method to use for such destruction, how much time is needed to achieve the destruction and how much energy will be required to do so.
[0004] The above is particularly important for certain food processing facilities such as for example those facilities processing chicken carcass products, wherein a manufacturer wants to preserve the skin on the carcass or body part for the end use customer, but must effectively destroy or kill any organisms on the skin.
SUMMARY OF THE INVENTION
[0005] According to the present embodiments, use of heat flux measurement for temperature control is more accurate and therefore more reliable than using a temperature probe or sensor to determine optimum conditions for destroying organisms.
[0006] The present embodiments include an apparatus to determine physiological effects of heat flux with a cryogenic substance applied to an organism, which apparatus includes a heat flux sensor; a cryogen delivery apparatus spaced apart a distance D1 from the heat flux sensor; and an organism culture supported on the heat flux sensor in a path of delivery of the cryogenic substance from the cryogen delivery apparatus to contact said culture.
[0007] The apparatus also includes the cryogen delivery apparatus is movable with respect to the heat flux sensor to alter the delivery path of the cryogenic substance and the distance D1 to the organism culture.
[0008] The apparatus further includes the cryogen delivery apparatus comprises a spray nozzle.
[0009] The apparatus further includes the spray nozzle emits a cryogen spray cone having a lower most edge at a distance D2 from the heat flux sensor.
[0010] The apparatus further includes the cryogenic substance is a cryogen selected from the group consisting of liquid nitrogen (LIN), liquid oxygen (LOX), and liquid argon.
[0011] The apparatus further includes the organism culture comprises Campylobacter.
[0012] The apparatus further includes a source of cryogen in fluid communication with the cryogen delivery apparatus.
[0013] There is also provided herein a method to determine physiological effects of heat flux with a cryogenic substance applied to an organism, which method includes testing an organism culture for heat flux with a cryogenic substance for establishing an amount of heat transfer required at the organism for achieving maximum destruction of said organism.
[0014] The method also includes the testing further comprises spraying the cryogenic substance onto the organism for different periods of time.
[0015] The method further includes the cryogenic substance is a cryogen selected from the group consisting of liquid nitrogen (LIN), liquid oxygen (LOX), and liquid argon.
[0016] The method further includes the testing comprises contacting the organism culture with the cryogenic substance; observing structural integrity of cells of said organism culture; adjusting a distance that the cryogenic substance travels for contacting the organism culture; adjusting an amount of the cryogenic substance applied to the organism culture; adjusting an amount of time the cryogenic substance is applied to the organism culture; and adjusting an amount of heat transfer (Wm2) which occurs from subjecting the organism culture to the cryogenic substance.
[0017] The method further includes the observing comprises measuring ATP leaking from cell walls of the organism.
[0018] The method further includes the testing is with a plurality of organism cultures.
[0019] The method further includes the adjusting the amount of the cryogenic substance comprises emitting the cryogenic substance in a cone shape for covering the organism culture.
[0020] The method further includes the organism culture comprises Campylobacter.
BRIEF DESCRIPTION OF THE DRAWINGS
[0021] For a more complete understanding of the present invention, reference may be had to the detailed description of the following embodiments taken in conjunction with the drawings, of which: [0022] FIG. 1 shows a schematic of a spray apparatus embodiment of the present invention; [0023] FIG. 2 shows a schematic of the apparatus for testing an organism culture; and [0024] FIG. 3 shows a graph depicting an example of organism destruction by embodiments of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
[0025] Before explaining the inventive embodiments in detail, it is to be understood that the invention is not limited in its application to the details of construction and arrangement of parts illustrated in the accompanying drawings, since the invention is capable of other embodiments and being practiced or carried out in various ways. Also, it is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation.
[0026] In the following description, terms such a horizontal, upright, vertical, above, below, beneath and the like, are used solely for the purpose of clarity illustrating the invention and should not be taken as words of limitation. The drawings are for the purpose of illustrating the invention and are not intended to be to scale.
[0027] Basically, apparatus and method embodiments are provided herein for testing live Campylobacter culture with heat flux instrumentation to establish an amount of heat transfer necessary in order to achieve maximum destruction of a Campylobacter cell on a cost-effective basis. The apparatus and method also determine a type of liquid nitrogen (LIN) spray jet required to do so, an amount of time required to destroy the Campylobacter, a distance that the LIN spray jet should be from the Campylobacter organism, and the LIN pressure required for the LIN spray jet to be provided in an optimum pattern. Such apparatus and method embodiments clearly establish operational parameters for control of a cooling or freezing tunnel different from known methods of process control.
[0028] Referring to FIG. 1, there is shown a spray rig apparatus 10 for testing a physiological effect of cryogen spray on an organism, such as for example a Campylobacter organism, in order to determine operational control of a freezer tunnel in which the organism will be exposed for destruction of same. A related method will be discussed hereinafter. The cryogen used can be liquid nitrogen (LIN), liquid oxygen (LOX) or liquid argon. For purposes of the disclosure herein, LIN will be used by way of example only. LIN and the other cryogen substances provide for rapid heat transfer of the Campylobacter and therefore, are efficient and cost-effective in destroying same. Reference herein to a freezer tunnel is by way of example only, and it is understood that the present embodiments can be used for other types of freezers, such as for example spiral freezers.
[0029] The spray rig apparatus 10 or apparatus includes a heat flux sensor 12 supported on a platform 14 or other supporting member, spaced apart from and for most applications beneath a cryogen delivery apparatus 16 such as for example a spray nozzle for producing a related spray 18 of the cryogen to contact the sensor. A clamp 20 or other mechanical fastener supports the nozzle 16 in position with respect to the heat flux sensor 12. The clamp 20 is adjustable to thereby adjust a direction of the LIN spray 18, especially with respect to the heat flux sensor 12. The heat flux sensor 12 is connected by a wire 22 to a controller (not shown) for transmitting data to the controller or other CPU (not shown). A tank 24 or vessel as constructed contains therein the cryogen substance such as for example the LIN which is distributed from the tank through a hose 26 in fluid communication with the cryogen spray nozzle 16. The tank 24 may have an internal volume sized to contain for example 200 liters (200L) of the LIN. The tank 24 is under pressure and the LIN upon exposure to ambient atmosphere at the spray nozzle 16 phase changes to a gaseous liquid spray. The pressure of the LIN through the hose 26 may be at 2 Barg, for example.
[0030] Referring now to FIG. 2, the apparatus 10 is shown for use with a Campylobacter culture 28 removably mounted on the heat flux sensor 12. The schematic of the apparatus for testing an organism culture is shown with the liquid nitrogen spray, height of a liquid nitrogen spray, and area of liquid nitrogen spray to determine best conditions to destroy most if not all of the organism in the culture. The Campylobacter culture 28 may be contained in a petri dish 30 or any other type of container suitably constructed for containing organism cultures therein. The petri dish 30 is supported on the heat flux sensor 12. A plurality of tests are carried out using the apparatus 10 to determine heat transfer rates of the cultures of Campylobacter which are exposed to the cryogen spray 18.
[0031] The purpose of the test apparatus 10 and related method is to determine (i) whether the cryogen or in this instance the LIN spray 18 disrupts the Campylobacter cells by viewing cell contents to determine if the cell contents have leaked from the cells (which determination can be done with a test to rapidly measure actively growing microorganisms through detection of adenosine triphosphate (ATP) leaking from the cells) after exposure to the LIN spray; (ii) an amount of Campylobacter destroyed when exposed to the spray 18 over different set points of time such as for example intervals of 5 or 10 seconds from an aggregated period of time from 10 to 40 seconds; (iii) a height “H” or distance D1 of an outlet of the spray nozzle 16 above the culture 28 (or alternatively a distance “D2” of a cone 19 of the spray 18 to the culture 28) to establish a best height and/or distance for maximum effect of the spray 18 on the cultured organism; and (iv) an amount of energy - watts per square meter (Wm2)- of heat transfer which occurs from the spray 18 and how same corresponds to the destruction of the Campylobacter organism. The cone shape 19 of the spray 18 facilitates covering the culture 28. All living organisms have cells, and all cells include therein ATP. Organisms other than Campylobacter can also be tested with the present embodiments. As soon as a cell wall is breached, the ATP will begin leaking out of the cell, and when a certain amount of the ATP has indeed leaked from the cell same will be destroyed. The rapid shock of the heat flux created by the extremely low, cryogenic temperature of the LIN breaches the cell wall causing the ATP to leak from the cell resulting in the destruction of same.
[0032] FIG. 3 shows a graph wherein the x-axis (abscissa) discloses the amount of time in seconds, while the y-axis (ordinate) discloses an amount of voltage used during the test. An Example follows.
[0033] Referring again to FIG. 2, a test for determining the parameters of the LIN spray to be applied to the Campylobacter will necessitate using a plurality of the cultures 28 each to be in a respective one of the petri dishes 30 in which a similar amount of the Campylobacter culture/organism is provided in each dish. The plurality of dishes 30 are each a “control” as long as each contains essentially the same type and amount of the organism. Thereafter, each dish is exposed under different conditions provided by the apparatus 10, resulting in a plurality of the petri dishes 30 being subjected to different operating parameters of the apparatus to determine the most effective combination of variables (i)-(iv) discussed above to destroy the organism. For example, petri dish # 1 holding a specific amount of Campylobacter culture 28 would be positioned on the heat flux sensor 12 and spray nozzle 16 set at a specific height H from the culture. Additionally, the pressure of the cryogen passing through the nozzle 16 would be so that the cone 19 of the cryogen spray would be at a specific distance D2 from the culture. Moreover, the amount of time in seconds would be selected for exposure of the culture 28 to the LIN spray. After the cryogenic substance (LIN) is applied to the culture 28, the petri dish #1 would be removed from the heat flux sensor 12, identified or captioned with the foregoing testing particulars and stored for comparison after additional petri dishes (petri dish #2, etc.) are placed on the heat flux sensor 12 with a different combination of height, distance and time. However, each of the subsequent petri dishes 30 (#2, etc.) would still contain the same type and amount of organism, such as Campylobacter used in the petri dish #1. After x-number of Campylobacter cultures 28 were tested, all would be compared under a microscope for determining which combination of height, distance, time and LIN spray most effectively destroyed the cultures. Such combination would be used as the optimum conditions necessary for the food freezer apparatus in order to efficiently destroy the Campylobacter on the surface of the food product. A further Example follows.
[0034] Example. A Campylobacter culture 28 is exposed to a LIN spray 18, which spray is 150 mm from the culture for 20, 30, and 42 second periods of time. During the periods of time, the organism will have a 99 to 99.9% reduction (i.e. be destroyed) because the organism will suffer from cell wall breakdown caused by exposure to the LIN spray. ATP leakage from the cell will occur into the surrounding area as the cell wall begins and continues to fail. This substantial destruction in the organism is caused by a cell wall of each failing or breaking down, which causes the ATP to leak from the organism and thereby be detected. The ATP leakage is used to measure the percentage of organism destroyed. ATP is present in all living organisms.
[0035] It was determined in this Example that the spray 18 positioned 150 mm distant from the culture 28 required 1938 Wm2 of continuous spray to enable destruction of the Campylobacter organism. Accordingly, this was determined to be the zone of destruction necessary for Campylobacter when processing same in a freezer tunnel that will destroy the organism, thereby better preserving the food product.
[0036] Referring still to FIG. 3, an amount of heat transfer is shown occurring at the culture over 100 seconds. It can be seen that the amount of heat transfer is constant indicating that the LIN spray is uniform and continuous. The Y axis displays micro-volts from the heat flux cell, which is converted to Wm2, while the X axis displays the time in seconds. The heat transfer (Wm2) produced over the time period shown by the graph by the LIN spray is sufficient to destroy the Campylobacter, otherwise known as the zone of destruction.
[0037] Although the present embodiments have been discussed with respect to the Campylobacter organism, it is understood that the present embodiments can be used with other types of organisms and food products in order to establish cooling rates for the optimum preservation of such products or destruction of organisms thereon.
[0038] It will be understood that the embodiments described herein are merely exemplary, and that one skilled in the art may make variations and modifications without departing from the spirit and scope of the invention. All such variations and modifications are intended to be included within the scope of the invention as described and claimed herein. Further, all embodiments disclosed are not necessarily in the alternative, as various embodiments of the invention may be combined to provide the desired result.
Claims (15)
1. An apparatus to determine physiological effects of heat flux with a cryogenic substance applied to an organism, comprising: a heat flux sensor; a cryogen delivery apparatus spaced apart a distance D1 from the heat flux sensor; and an organism culture supported on the heat flux sensor in a path of delivery of the cryogenic substance from the cryogen delivery apparatus to contact said culture.
2. The apparatus of claim 1, wherein the cryogen delivery apparatus is movable with respect to the heat flux sensor to alter the delivery path of the cryogenic substance and the distance D1 to the organism culture.
3. The apparatus of claim 2, wherein the cryogen delivery apparatus comprises a spray nozzle.
4. The apparatus of claim 3, wherein the spray nozzle emits a cryogen spray cone having a lower most edge at a distance D2 from the heat flux sensor.
5. The apparatus of claim 1, wherein the cryogenic substance is a cryogen selected from the group consisting of liquid nitrogen (LIN), liquid oxygen (LOX), and liquid argon.
6. The apparatus of claim 1, further comprising a source of cryogen in fluid communication with the cryogen delivery apparatus.
7. The apparatus of claim 1, wherein the organism culture comprises Campylobacter.
8. A method to determine physiological effects of heat flux with a cryogenic substance applied to an organism, comprising: testing an organism culture for heat flux with a cryogenic substance; and establishing an amount of heat transfer required at the organism for achieving maximum destruction of said organism.
9. The method of claim 8, wherein the testing further comprises spraying the cryogenic substance onto the organism for different periods of time.
10. The method of claim 8, wherein the cryogenic substance is a cryogen selected from the group consisting of liquid nitrogen (LIN), liquid oxygen (LOX), and liquid argon.
11. The method of claim 8, wherein the testing comprises: contacting the organism culture with the cryogenic substance; observing structural integrity of cells of said organism culture; adjusting a distance that the cryogenic substance travels for contacting the organism culture; adjusting an amount of the cryogenic subtance applied to the organism culture; adjusting an amount of time the cryogenic substance is applied to the organism culture; and adjusting an amount of heat transfer (Wm2) which occurs from subjecting the organism culture to the cryogenic substance.
12. The method of claim 11, wherein the observing comprises measuring ATP leaking from cell walls of the organism.
13. The method of claim 11, wherein the testing is with a plurality of organism cultures.
14. The method of claim 11, wherein the adjusting the amount of the cryogenic substance comprises emitting the cryogenic substance in a cone shape for covering the organism culture.
15. The method of claim 8, wherein the organism culture comprises Campylobacter.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1520478.7A GB2544537B (en) | 2015-11-20 | 2015-11-20 | Apparatus and method to determine physiological effects of cryogen on organisms for controlling freezers |
| PCT/EP2016/078211 WO2017085300A1 (en) | 2015-11-20 | 2016-11-18 | Apparatus and method to determine physiological effects of heat flux with cryogen applied to organisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1520478.7A GB2544537B (en) | 2015-11-20 | 2015-11-20 | Apparatus and method to determine physiological effects of cryogen on organisms for controlling freezers |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB201520478D0 GB201520478D0 (en) | 2016-01-06 |
| GB2544537A true GB2544537A (en) | 2017-05-24 |
| GB2544537B GB2544537B (en) | 2020-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| GB1520478.7A Expired - Fee Related GB2544537B (en) | 2015-11-20 | 2015-11-20 | Apparatus and method to determine physiological effects of cryogen on organisms for controlling freezers |
Country Status (2)
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| GB (1) | GB2544537B (en) |
| WO (1) | WO2017085300A1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6669688B2 (en) * | 2000-01-25 | 2003-12-30 | The Regents Of The University Of California | Method and apparatus for measuring the heat transfer coefficient during cryogen spray cooling of tissue |
| US20040053204A1 (en) * | 2000-06-07 | 2004-03-18 | Morris George J. | Methods and apparatus for freezing tissue |
| EP3000332A1 (en) * | 2014-09-23 | 2016-03-30 | Linde Aktiengesellschaft | Apparatus and method for determining heat flux in an atmosphere of a freezer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| SU1455184A1 (en) * | 1987-06-30 | 1989-01-30 | Специальное Конструкторско-Технологическое Бюро С Опытным Производством Института Проблем Криобиологии И Криомедицины Ан Усср | Apparatus for programmed freezing of biological objects |
| US5450732A (en) * | 1994-04-29 | 1995-09-19 | Liquid Carbonic Corporation | Distribution system for cryogen |
| GB9501403D0 (en) * | 1995-01-25 | 1995-03-15 | Boc Group Plc | A food freezing apparatus |
| US20110151088A1 (en) * | 2009-12-22 | 2011-06-23 | Newman Michael D | Heat flux freezer control apparatus and method |
| GB2513451B (en) * | 2011-06-07 | 2015-11-04 | Matthews Bernard Ltd | Food hygiene method and food product |
| US20160030607A1 (en) * | 2014-08-04 | 2016-02-04 | Michael D. Newman | Heat flux control for liquid nitrogen sprays |
| EP2998668A1 (en) * | 2014-09-17 | 2016-03-23 | Linde Aktiengesellschaft | Apparatus and method for applying a heat transfer substance |
| GB2540218A (en) * | 2015-07-06 | 2017-01-11 | Linde Ag | Heat flux control tunnel for food preservation and removal of micro-organisms |
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2015
- 2015-11-20 GB GB1520478.7A patent/GB2544537B/en not_active Expired - Fee Related
-
2016
- 2016-11-18 WO PCT/EP2016/078211 patent/WO2017085300A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6669688B2 (en) * | 2000-01-25 | 2003-12-30 | The Regents Of The University Of California | Method and apparatus for measuring the heat transfer coefficient during cryogen spray cooling of tissue |
| US20040053204A1 (en) * | 2000-06-07 | 2004-03-18 | Morris George J. | Methods and apparatus for freezing tissue |
| EP3000332A1 (en) * | 2014-09-23 | 2016-03-30 | Linde Aktiengesellschaft | Apparatus and method for determining heat flux in an atmosphere of a freezer |
Non-Patent Citations (3)
| Title |
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| International Journal of Food Science & Technology (1989); Vol 24, pp 243-260, "A model for heat transfer in cryogenic food freezing", Awonorin * |
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Also Published As
| Publication number | Publication date |
|---|---|
| GB201520478D0 (en) | 2016-01-06 |
| GB2544537B (en) | 2020-06-17 |
| WO2017085300A1 (en) | 2017-05-26 |
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