GB2543713A - Polymeric proteins and uses thereof - Google Patents

Polymeric proteins and uses thereof

Info

Publication number
GB2543713A
GB2543713A GB1702637.8A GB201702637A GB2543713A GB 2543713 A GB2543713 A GB 2543713A GB 201702637 A GB201702637 A GB 201702637A GB 2543713 A GB2543713 A GB 2543713A
Authority
GB
United Kingdom
Prior art keywords
immunoglobulin
proteins
heavy chain
chain constant
polypeptide monomer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1702637.8A
Other versions
GB201702637D0 (en
GB2543713B (en
Inventor
Pleass Richard
Blundell Patricia
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Liverpool School of Tropical Medicine
Original Assignee
Liverpool School of Tropical Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Liverpool School of Tropical Medicine filed Critical Liverpool School of Tropical Medicine
Publication of GB201702637D0 publication Critical patent/GB201702637D0/en
Publication of GB2543713A publication Critical patent/GB2543713A/en
Application granted granted Critical
Publication of GB2543713B publication Critical patent/GB2543713B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/35—Valency
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/524—CH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C07K2317/53—Hinge
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
    • C07K2317/94—Stability, e.g. half-life, pH, temperature or enzyme-resistance
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Provided are polymeric proteins that comprise two or more polypeptide monomer units, each monomer unit comprising two chimeric protein chains. Each chimeric polypeptide monomer unit comprises an Fc receptor binding portion comprising two immunoglobulin G heavy chain constant regions,wherein each immunoglobulin G heavy chain constant region comprises a cysteine residue which is linked via a disulfide bond to a cysteine residue of an immunoglobulin G heavy chain constant region of an adjacent polypeptide monomer unit. Each chimeric protein chain also comprises a modified immunoglobulin M tailpiece region, wherein the amino acid sequence of each chimeric polypeptide monomer comprises an alteration of the primary structure as compared to the native sequences from which the immunoglobulin G heavy chain constant region or immunoglobulin M tailpiece region are derived, and the alteration changes the number of glycosylation sites in a manner that promotes polymerisation. This promotion of polymerisation may lead to the generation of tetrameric, hexameric, and even dodecameric forms of the proteins. The proteins are suitable formedical uses,such as in the prevention or treatment of autoimmune diseases such as idiopathic thrombocytopenia. Also provided are methods of treatment using the polymeric proteins, and pharmaceutical compositions comprising the polymeric proteins.
GB1702637.8A 2014-07-18 2015-07-20 Polymeric proteins and uses thereof Active GB2543713B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1412821.9A GB201412821D0 (en) 2014-07-18 2014-07-18 Polymeric proteins and uses thereof
PCT/GB2015/052098 WO2016009232A1 (en) 2014-07-18 2015-07-20 Polymeric proteins and uses thereof

Publications (3)

Publication Number Publication Date
GB201702637D0 GB201702637D0 (en) 2017-04-05
GB2543713A true GB2543713A (en) 2017-04-26
GB2543713B GB2543713B (en) 2019-03-27

Family

ID=51494824

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB1412821.9A Ceased GB201412821D0 (en) 2014-07-18 2014-07-18 Polymeric proteins and uses thereof
GB1702637.8A Active GB2543713B (en) 2014-07-18 2015-07-20 Polymeric proteins and uses thereof

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB1412821.9A Ceased GB201412821D0 (en) 2014-07-18 2014-07-18 Polymeric proteins and uses thereof

Country Status (2)

Country Link
GB (2) GB201412821D0 (en)
WO (1) WO2016009232A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11034775B2 (en) 2016-06-07 2021-06-15 Gliknik Inc. Cysteine-optimized stradomers
SG11201900427PA (en) 2016-07-22 2019-02-27 Gliknik Inc Fusion proteins of human protein fragments to create orderly multimerized immunoglobulin fc compositions with enhanced fc receptor binding
US11331372B2 (en) 2016-12-09 2022-05-17 Gliknik Inc. Methods of treating inflammatory disorders with multivalent Fc compounds
WO2019175605A1 (en) * 2018-03-16 2019-09-19 Liverpool School Of Tropical Medicine Chimeric fc receptor binding proteins and uses thereof
CN117545767A (en) * 2021-06-17 2024-02-09 菲鹏生物股份有限公司 Chimeric immunoglobulins

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011073692A1 (en) * 2009-12-18 2011-06-23 The University Of Nottingham Proteins, nucleic acid molecules and compositions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011073692A1 (en) * 2009-12-18 2011-06-23 The University Of Nottingham Proteins, nucleic acid molecules and compositions

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COLOMA M J, CLIFT A, WIMS L, MORRISON S L: "The role of carbohydrate in the assembly and function of polymeric IgG.", MOLECULAR IMMUNOLOGY., PERGAMON, GB, vol. 37, no. 18, 1 December 2000 (2000-12-01), GB, pages 1081 - 1090, XP002745729, ISSN: 0161-5890, DOI: 10.1016/S0161-5890(01)00024-4 *
Nature Biotech., Vol.12, 1994, Smith, R. I. et al., "Recombinant polymeric IgG...", pp.683-688 *

Also Published As

Publication number Publication date
GB201702637D0 (en) 2017-04-05
WO2016009232A1 (en) 2016-01-21
GB2543713B (en) 2019-03-27
GB201412821D0 (en) 2014-09-03

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