GB2540116A

GB2540116A - Novel peptide

Info

Publication number: GB2540116A
Application number: GB1506316.7A
Authority: GB
Inventors: Phopase Jaywant
Original assignee: UAB FERENTIS
Current assignee: UAB FERENTIS
Priority date: 2015-04-14
Filing date: 2015-04-14
Publication date: 2017-01-11
Anticipated expiration: 2035-04-14
Also published as: GB2540116B; GB201506316D0

Abstract

A peptide comprising an amino acid sequence wherein said peptide comprises four amino acid motifs represented in the following formula: (Xaa1 Xaa2)w (Xaa3- Xaa4 -Xaa5)x (Xaa6-Xaa7-Xaa8)y (Xaa9-Xaa10-Xaa11)z where Xaa1 is a thiol containing amino acid and Xaa2 is glycine and w comprises at least one repeat of the first amino acid motif; Xaa3 is proline, Xaa4 is lysine, Xaa5 is glycine and x comprises 1-4 repeats of the second amino acid motif; Xaa6 is proline, Xaa7 is hydroxyproline (Hyp), Xaa8 is glycine and y comprises 1-4 repeats of the third amino acid motif; and Xaa9 is aspartic acid or glutamic acid, Xaa10 is hydroxyproline, Xaa11 is glycine and z comprises 1-4 repeats ofthe fourth amino acid motif. The peptide may comprise the sequence Cys-Gly-(Pro-Lys-Gly)4(Pro-Hyp-Gly)4(Asp-Hyp-Gly)4. The peptide is a collagen mimetic peptide (CMP) that mimics the higher order triple helical self-assembly of a collagen. Also claimed is a hydrogel comprising said CMP, optionally comprising collagen and optionally comprising a chemically modified surface; a corneal implant based on said hydrogel/modified hydrogel comprising a transparent central portion formed by an interpenetrating network comprising one or more additional biocompatible polymers wherein the central portion is adapted to remain cell free to ensure unhindered transmission of light.

Description

Novel Peptide

Field of the Invention

This disclosure relates to a novel Collagen Mimetic Peptide; a multi-arm conjugate comprising said peptide that mimics the higher order triple helical self-assembly of a collagen; a hydrogel comprising said Collagen Mimetic Peptide, optionally comprising collagen and optionally comprising a chemically modified surface; a corneal implant based on said hydrogel/modified hydrogel comprising a transparent central portion formed by an interpenetrating network comprising one or more additional biocompatible polymers wherein the central portion is adapted to remain cell free to ensure unhindered transmission of light.

Advantageously, the implant is tolerated by the immune system, has minimal side effects and when applied to a damaged or diseased eye regenerates corneal tissue.

Background of the Invention

The ability to see is dependent on the actions of several structures in and around the eye. When one focuses on an object, light rays are reflected from the object to the cornea. The light rays are then bent, refracted and focused by the cornea into the eyeball, through the lens and final to the retina. The cornea is the main refractive element in the eye and is responsible for 75-80% of the focussing of light onto the retina. The retina then converts light into electrical impulses which are transmitted through the optic nerve to the brain where the image is perceived. The cornea is a highly organised structure being composed of 3 cellular layers - an external multilayered epithelium, middle stroma, and inner corneal endothelium. The acellular largely collagenous Bowman’s membrane underlies the epithelium and is often considered tha anteriormost portion of the stroma. Between the stroma and endothelium, lies a thinner acellular layer, the Descemet’s membrane.

There are a large number of diseases and conditions that affect the function of the cornea requiring transplantation of corneal tissue from donors. Diseases such as Fuchs' dystrophy, iridocorneal endothelial syndrome, keratoconus, lattice dystrophy, ocular herpes infections, trachoma are examples. In addition physical damage to the eye, for example chemical burns, sports injuries, and shrapnel injuries during military conflict are common due to the vulnerable nature of the eye.

Existing available surgical treatments include penetrating keratoplasty (full-thickness transplantation), anterior lamellar keratoplasty (and deep lamellar keratoplasty, which removes the epithelium and damaged stroma and spares the deepest parts of the stroma and endothelium) and endothelial keratoplasty (by which the patient's endothelium is replaced with a transplanted disc of posterior stroma/Descemet’s membrane/corneal endothelium or Descemet’s membrane/corneal endothelium). Although corneal transplantations are increasingly successful, there is a worldwide shortage of donor organs and current supplies cannot meet the demand. Moreover, patients with severe pathologies such as chemical burns, severe infections, autoimmune conditions, have a high risk of rejecting transplanted human donor corneas. Even allografting of a single epithelial layer from stem cells bears a lifelong risk of rejection necessitating the recipient to take expensive immune-suppressant drugs with often severe adverse side effects.

The extreme shortage of donor corneas, the high risk of rejection and infection after transplantation has led to a long felt need to design materials with cornea-like characteristics which are suitable for use as prosthetics or for implantation. Materials suitable as implants must be transparent (allowing more than 85% of light transmission), of good mechanical strength to allow handling, stable against enzymatic digestion, allow cell adhesion and migration to support regeneration. Examples of attempts to design corneal implants and prosthesis are known in the prior art.

For example, W02006042272 discloses materials suitable to artificially replace or augment a damaged or diseased cornea comprising a hydrogel based on biocompatible polymers allowing diffusion of nutrients and improved mechanical strength. The artificial cornea also comprises molecules such as proteins and peptides, for example collagen, which are covalently linked to the surface of the hydrogel to promote epithelial cell adhesion and proliferation on the non-adhesive hydrogel surface. EP2535041 discloses an interpenetrating polymeric network of two or more polymer networks creating a hydrogel matrix wherein at least one polymer is a biopolymer such as collagen. This hydrogel is biocompatible, nontoxic and suitable for use as a scaffold for tissue regeneration. Liu et al (2009) discloses collagen phoshorylcholine interpenetrating network hydrogels as corneal substitutes. Artificial corneas, transplanted into mini-pigs, were shown to promote re-epithelialization and nerve regeneration. Others such as US2008/0287342 have developed corneal shields consisting of a network comprising collagen-mimetic peptide-PEG polymer conjugates to deliver drugs and to provide a protective environment that promotes the healing or surgical and traumatic wounds.

Although artificial corneas based on collagen are cell friendly and allow adhesion and cell proliferation they may not be suitable as a permanent implant in all patients, particularly those with very severe pathologies (e.g. chemical burns, dry eye, infections, autoimmune disease), where there is abnormal cell growth and vascularisation can reduce transparency, which can also grow into the artificial cornea when grafted into such eyes. The cornea is an avascular structure and several attempts to mimic the natural corneal characteristics through “skirt and core” matrices aimed to provide both a translucent core and an outer skirt which enables vascularisation and cell migration. For example, US2011/0125260 discloses an artificial cornea comprising a ridged translucent core and a skirt comprising a porous hydrogel, wherein the core is made by forming a hydrogel skirt around a mould wherein the mould is filled with synthetic monomers such as poly(methyl methacrylate) forming a core. The boundary of skirt/core forms an Interpenetrating network and the core is optionally covered by a bacterial resistant biofilm. The hydrogel skirt comprises biological molecules such as peptides, proteins or collagen. US2011/182968 discloses a comeal prosthesis comprising a core and skirt. The core comprises an interpenetrating network comprising PEG-DA (first network) and methacrylic acid (second network) whereas the skirt is hydrogel based and can contain biomolecules linked to it. Biomolecules such as peptides or collagen can be incorporated into the interpenetrating network of the core by forming acrylate-PEG-peptide monomers which can then be linked to PEGDA of the first network and acrylates of the second network of the core. Myung et al (2007) discloses artificial corneas comprising a central core composed of a polyethylene glycol)/poly(acrylic acid) (PEG/PAA) double network with collagen type I tethered to its surface, and a micro-perforated PHEA hydrogel skirt also modified with collagen. The interpenetrating micro-perforated skirt promotes stromal tissue integration whereas the double network core supports surface epithelialization. US2014/0142200 discloses a double-crosslinked, transparent collagen material for use as an ophthalmic device, wherein the collagen is diafiltered, lyophilised, redissolved and homogenized and non-fibrillar to reduce small molecule contaminants and subsequently obtaining accurate collagen concentrations essential for an efficient cross-linking method.

Although collagen based interpenetrating network hydrogels are advantageous over synthetic interpenetrating network hydrogels by allowing superior cell adhesion and migration, the production of recombinant collagen is exceedingly expensive making it a less suitable alternative when considering mass production of artificial corneas. Additionally, the production of an artificial cornea comprising skirt and core structure requires several steps thus increasing the cost. Moreover, none of the above attempts to provide a replacement cornea has successfully fully integrated the artificial implant that replaces a damaged or diseased cornea with a fully functional artificial cornea. To date, clinical used prostheses are non-immune compatible and necessitate live-long immunospression. Furthermore, increased intraocular pressure leading to glauvoma is a severe side effect that often necessitates coimplantation of a shunt to prevent glaucoma; [see Management of Glaucoma Following Boston Keratoprosthesis: Gargi Khare Vora , Kathryn A Colby European Ophthalmic

Review, 2012;6(4):214~7.

In our co-pending PCT applications, WO2015/032985, PCT/EP2014/072032 [currently unpublished] and PCT/EP2014/072025 [currently unpublished] we disclose a hydrogel, a modified hydrogel and a corneal implant respectively. The content of each PCT application is incorporated by reference in their entirety.

This disclosure relates to a hydrogel comprising a novel collagen mimetic peptide (which can also be described as a collagen-like peptide) that interlinks to form a network which allows migration of cells and vessel ingrowth aiding regeneration into the surrounding tissue minimising the risk of rejection when used in a corneal implant. Moreover, the artificial cornea comprises a core that prevents cell and vessel ingrowth so maintaining optimal transparency. The use of the novel collagen mimetic peptide forming the network of the hydrogel is advantageous as it allows great flexibility converting an inert polymeric backbone (plastic or biopolymer such as silk) and reduces the costs of producing the artificial corneas significantly compared to use of recombinant human collagen due to reduction of the purification steps; and compared to extracted animal source polymers such as collagen, the costs of batch-to-batch testing for pathogens to prevent transmission in extracted materials. The device is of substantial mechanical strength and elasticity allowing additional surface modification. Kits allowing tailor made skirt core matrices are also disclosed.

Statement of the Invention

According to an aspect of the invention there is provided a peptide comprising an amino acid sequence wherein said peptide comprises at least four amino acid motifs represented in the following formula:

(Xaai is a thiol containing amino acid and Xaa2 is a glycine amino acid wherein said w comprises at least one repeat of said first motif, (Xaa3- Xaa4 -Xaa5)x is a second amino acid motif wherein Xaa3 is proline, Xaa4 is lysine, Xaa5 is glycine wherein x comprises 1-4 repeats of said second amino acid motif, (Xaa6-Xaa7-Xaa8)y is a third amino acid motif wherein Xaa6is proline, Xaa7 is hydroxyproline, Xaa8is glycine wherein y comprises 1-4 repeats of said third amino acid motif; and (Xaag-Xaa^-Xaa^) is a fourth amino acid motif wherein Xaa9 is aspartic acid or glutamic acid, Xaa10 is hydroxyproline, Xaa^ is glycine wherein z comprises 1-4 repeats of said fourth amino acid motif.

In a preferred embodiment of the invention said thiol containing amino acid is cysteine.

In a preferred embodiment of the invention said first amino acid motif comprises more than 1 repeat; preferably said peptide comprises 1, 2, 3 or 4 repeats of said first amino acid motif.

In a preferred embodiment of the invention said peptide comprises at least, 1, 2, 3 or 4 repeats of said second amino acid motif.

In a preferred embodiment of the invention said peptide comprises at least 1, 2, 3 or 4 repeats of said third amino acid motif.

In a further preferred embodiment of the invention said peptide comprises at least 1, 2, 3 or 4 repeats of said fourth amino acid motif.

In a preferred embodiment of then invention said peptide comprise the amino acid sequence

wherein Xaa^ is a natural or modified thiol containing amino acid.

In a preferred embodiment of the invention said peptide comprises the amino acid sequence

wherein said peptide is a collagen mimetic peptide.

In a preferred embodiment of the invention said peptide is at least 11 amino acids in length; preferably said peptide comprises 11 to 38 amino acids.

In a preferred embodiment of the invention said peptide is greater than 38 amino acids in length; preferably, between 38 amino acids and 100 amino acids.

In a preferred embodiment of the invention said peptide is derivatized by chemical modification to provide one or more reactive groups.

In a preferred embodiment of the invention said peptide is modified by addition of one or more functional groups selected from the group consisting of: thiol, methyacrylate or acrylate functional groups.

In a preferred embodiment of the invention said modified peptide comprises polyethylene glycol; preferably polyethylene glycol-maleimide or polyethylene glycol diacrylate or polyethylene glycol methacrylate.

Preferably said polyethylene glycol-maleimide is at least 2, 4, 6 or 8 arm polyethylene glycol-maleimide.

According to a further aspect of the invention there is provided a hydrogel comprising: a plurality of modified collagen mimetic peptides according to the invention chemically cross linked into a network.

In a preferred embodiment said modified collagen mimetic peptide is activated via N-ethyl-N'-[3-dimethylaminopropyl] carbodiimide/N-hydroxy succinimide (EDC/NHS).

In a preferred embodiment of the invention said hydrogel comprises one or more natural or synthetic biopolymers chemically cross linked to said network.

In a preferred embodiment of the invention said natural polymer is a collagen.

In a preferred embodiment of the invention said collagen is selected from the group consisting of: collagen I, collagen II, collagen III, collagen IV or collagen V, or mixtures thereof.

In an alternative preferred embodiment of the invention said natural polymer is selected from the group consisting of: fibrin, cell-interactive proteins, for example laminin, fibronectin, hyaluronic acid, chitosan, collagen mimetic peptides of different sequence to the collagen mimetic peptide according to the invention.

In a preferred embodiment of the invention said synthetic polymer is selected from the group consisting of: functionalized polyethylene glycol (PEG), polyvinyl alcohol (PVA), polyethylene glycol-diacrylate (PEGDA), PEG methacrylate (PEGMA), poly(hydroxyethyl methacrylate) (pHEMA), polyethylene glycol methyl ether methacrylate (PEGMEM), poly(pentaerythritol triacrylate) or poly(N-isopropylacryl amide) (PNIPAAm).

In a further preferred embodiment of the invention said network comprises a photoinitiator, for example Irgacure 2959.

In a preferred embodiment of the invention said network is photo-crosslinked.

In a preferred embodiment of the invention the elastic modulus of the hydrogel is at least 1 MPa or at least 5 MPa.

In a preferred embodiment of the invention said hydrogel is substantially transparent and allows a light transmission of at least 80 or 90% of light in the range of 400-700nm

In a preferred embodiment of the invention the total concentration of polymers in the hydrogel is at least 2 weight% or more.

According to a further aspect of the invention there is provided a method of manufacturing a hydrogel comprising the steps: i) providing a preparation comprising a modified peptide according to the invention; ii) providing a preparation comprising one or more biocompatible polymers and/or monomers wherein said polymers are provided with one or more functional groups; iii) combining preparations i) and ii) to provide a reaction mixture and contacting said reaction mixture with a crosslinking agent. iv) incubating the reaction mixture to crosslink said modified peptide with one or more biocompatible polymers and/or monomers to provide a cross-linked network.

In a preferred method of the invention said functional groups are selected from the group consisting of: thiol, acrylate and/or methacrylate, or synthetic monomers having thiol, acrylate and/or methacrylate functional groups.

In a preferred method of the invention said mixture of first and second preparation has a total polymer concentration of at least 2 weight%; preferable 12 weight%

In a preferred method of the invention said cross-linking agent is a chemical cross-linker.

In an alternative preferred method of the invention said cross-linking agent is a photoinitiator and UV radiation.

According to a further aspect of the invention there is provided a hydrogel according to the invention wherein said hydrogel is modified to provide a uniformly functionalized or patterned surface to which cells and/or biocompatible polymers/monomers adhere to modulate cell contact.

In a preferred embodiment of the invention said modulation is enhancement of cell adhesion and proliferation.

In an alternative embodiment of the invention said modulation is inhibition of cell adhesion.

In the present disclosure the hydrogel is formed by reacting firstly functionalised PEG optionally comprising multiple arms with a modified collagen mimetic peptide. The pegylated peptides are then subsequently cross linked by either chemical activation or UV crosslinking using a photo initiator. Further polymers can then be crosslinked again using chemical activation or a UV photoinitiator optionally forming a second network if desired. Optionally, further functionalization of the hydrogel surface can be achieved by providing a PEG-MA coating or chemical activation of the peptide carbonyl or other groups.

In the present disclosure the term “pattern” encompasses physical, chemical, or topographic, 2D or 3D, patterns. The present disclosure includes “surface modifying” the hydrogel according to the invention wherein the hydrogel has mechanical properties that allows such modifications. In order for the hydrogels to be surface modified according to the present invention it is preferable if the hydrogel has an elastic modulus of at least 1 MPa or at least 2MPa, or at least 3MPa, or at least 4MPa, or at least 5 MPa. The hydrogel according to the present invention contains at least 80wt% water, or at least 85wt% water, or at least 90wt% water, for example 85-90wt% water.

The hydrogel according to the invention combined with surface modification/patterning enhances for example tissue regenerative properties of the hydrogel but may also be used for construction of in vitro tissue models, arrays or tissue on a chip devices. For example, fibronectin patterns of relatively narrow stripes/lines, said stripes may further stimulate epithelial cell proliferation on a material that supports tissue regeneration. To use contact printing, photolithography on hydrogels is known. However, prior art discloses difficulties to produce well defined patterns and the methods are usually not suitable for large scale production. The present disclosure illustrates that even aqueous material may be used for contact printing or photolithography for example to prepare a surface pattern on the material. Prior art have shown that when photolithography on gels in the dry collapse state the pattern may be distorted/damaged when the gels subsequently are rehydrated in buffer. Moreover, to perform photolithography in water is expensive and technically difficult, not compatible with most of the production facilities. Instead of such complicated processes, by using a material that withstands contact printing or photolithography the present disclosure makes it possible to prepare hydrogels containing 2D patterns/3D patterns in a large scale production.

According to a further aspect of the invention there is provided a method for the modification of a hydrogel comprising: i) providing a hydrogel according to the invention; ii) applying to the surface of said hydrogel one or more monomeric or polymeric agents to modify the surface of the hydrogel; and/or iii) treating the applied surface compounds by micro-contact printing or photolithography or inkjet printing, or any other fabrication technique in order to create a pattern of said surface compounds.

In a preferred method of the invention said agents are selected from the group consisting of: polyethylene glycol (PEG)-acrylate polymer/monomers, PEG-methacrylate polymer/monomers, other acrylate, methacrylate, carboxyl, amino, amide, epoxide, hydroxyl, cyano, nitride, sulfonamido, acetylenyl, alkene, esters like imidoesters( N-hydroxysuccinimide ester) or pentafluorphenol ester or other, azide, thiol, maleimide, functionalized PEG derivatives, any bifunctional or multifunctional compounds, drugs, bioactive substances, biological molecules including proteins or peptides to said surface.

In a preferred embodiment of the invention said agent is a cell adhesion or cell modulating protein.

In a preferred method of the invention said agent is selected from the group consisting of: fibronectin, laminin, vitronectin or peptide motifs derived from these.

In a preferred method of the invention the pattern is in the shape of lines or dots, or any other geometrical figures having a width or diameter or any other geometrical parameter of at least 10 nm or more.

In a preferred method of the invention the width or diameter or any other geometrical figures of the pattern is from 1 pm or more.

According to an aspect of the invention there is provided a modified hydrogel obtained or obtainable by the method according to the invention.

In a preferred embodiment of the invention said hydrogel or modified hydrogel comprises at least one cell type.

In a preferred embodiment of the invention said cell type is a mammalian cell; preferably a human cell, for example an epithelial cell or mesenchymal-like stromal cells.

In a preferred embodiment of the invention said cell type is a stem cell.

The term “stem cell” represents a generic group of undifferentiated cells that possess the capacity for self-renewal while retaining varying potentials to form differentiated cells and tissues. Stem cells can be pluripotent or multipotent. A pluripotent stem cell is a cell that has the ability to form all tissues found in an intact organism although the pluripotent stem cell cannot form an intact organism. Furthermore, it is known that human somatic cells can be re-programmed to an undifferentiated state similar to an embryonic stem cell. For example, W02007/069666, the content of which is incorporated by reference in its entirety, describes re-programming of differentiated cells (e.g. mouse fibroblast cells) without the need to use embryonic stem cells. A multipotent cell has a restricted ability to form differentiated cells and tissues. Typically, adult stem cells are multipotent stem cells and are the precursor stem cells or lineage restricted stem cells that have the ability to form some cells or tissues and replenish senescing or damaged cells/tissues. Generally they cannot form all tissues found in an organism, although some reports have claimed a greater potential for such ‘adult’ stem cells than originally thought. Examples of multipotent stem cells include mesenchymal stem cells. Mesenchymal stem cells differentiate into a variety of cell types that include osteoblasts, chondrocytes, myocytes, adipocytes and neurones. Typically, mesenchymal stem cells are obtained from bone marrow. Currently, stem cell therapies are exploring different sources of pluripotent and multipotent stem cells and cell culture conditions to efficiently differentiate stem cells into cells and tissues suitable for use in tissue repair.

In a preferred embodiment of the invention said stem cell is a corneal stem cell or progenitor cell.

In a preferred embodiment of the invention said stem cell is a corneal endothelial cell or progenitor cell.

According to a further aspect of the invention there is provided a corneal implant comprising a hydrogel according to the invention.

In a preferred embodiment of the invention said corneal implant comprises a matrix part comprising a hydrogel according to the invention and a core part wherein the core part is substantially centrally located in the matrix part and comprises polymerized olefinic or UV polymerizable monomers, or a mixture thereof, the hydrogel of the matrix material and one or more antifouling polymers/agents characterised in that the core part is substantially transparent and free of cells/vessels.

In a preferred embodiment of the invention the core material comprises: HEMA and PEGMEM or HEMA and 2-methacryloyloxyethyl phosphorylcholine (MPC).

In a preferred embodiment of the invention said polymerized olefinic or UV polymerizable monomers are selected from the group: methyacrylate, methylmethacrylate, poly methyl methacrylate (PMMA), poly-hydroxyethyl methacrylate (pHEMA), pHEMA-poly ethylene glycol methacrylate or pHEMA-PEGMEM.

In a preferred embodiment of the invention the surface of the matrix material and/or the core material is modified and/or patterned with PEG-MA.

In a preferred embodiment of the invention the surface of the matrix material and/or the core material is activated using NHS/EDC.

In a preferred embodiment of the invention the surface of the matrix material and/or the core material is modified and/or patterned with N-hydroxysulfosuccinimide (NHS), polypeptides such as YIGSR, IKVAV, RGD, ECM proteins, fibronectin derived peptides, combinations of synergistic peptides, DGEA peptide from collagen, antibodies, glycosaminoglucans, motifs from growth factors, or pharmaceutically active substances.

In a preferred embodiment of the invention the weight ratio of core material is 1 wt% or more, or 5 wt% or more, or 15 wt% or more, or 30 wt% or more, or 50 wt% or more, or 95 wt% or less, or 85 wt% or less, or 75 wt% or less, or 65 wt% or less, or 55 wt% or less, of the total weight of the product.

In a preferred embodiment of the invention the matrix material comprises at least the collagen mimetic peptide according to the invention modified with polyethylene glycol wherein the PEG has 2, 4, 6 or 8 arms; preferably 8 arms

In a preferred embodiment of the invention said core material comprises HEMA and/or PEGMEM, or HEMA and/or MPC.

According to a yet further aspect of the invention there is provided a method for the manufacture of an implant according to the invention comprising: i) providing a matrix polymer and a cross-linker in a suitable solvent; ii) cross-linking the matrix polymer to form the matrix material; iii) providing core olefinic monomers or UV polymerizable monomers or polypeptides or a mixtures thereof, and an initiator, optionally in a suitable solvent; iv) adding the core olefinic monomers or UV polymerizable monomers or polypeptides, or a mixtures thereof and the initiator to the surface or the body of the matrix material; and v) allowing the core olefinic monomers to polymerize for a suitable period of time.

In a preferred method of the invention the olefinic based material is left to diffuse after addition in at least 15 minutes.

In a further preferred method of the invention the curing of said core is obtained during UV radiation using a mask.

According to a further aspect of the invention there is provided an implant according to the invention for use in the repair or replacement of disease or damaged corneal tissue.

According to a further aspect of the invention there is provided a surgical method for the repair or replacement of diseased or damaged corneal tissue in a subject in need of corneal repair or damage comprising: i) providing a corneal implant according to the invention; ii) attaching the corneal implant to the eye of said subject; and optionally iii) providing a protective covering to the repaired eye to facilitate healing.

In a preferred embodiment of the invention said use or surgical method is the repair or replacement of diseased corneal tissue wherein said disease is selected from the group consisting of: Fuchs' Dystrophy, iridocorneal endothelial syndrome, keratoconus, lattice dystrophy, ocular herpes infections, trachoma as examples.

In an alternative embodiment of the invention said use or surgical method is the repair or replacement of damaged corneal tissue wherein said damage is chemical damage, physical injury during sport or during military conflict.

According to a further aspect of the invention there is provided a hydrogel or modified hydrogel according to the invention for use in cell and tissue culture.

According to a further aspect of the invention there is provided a hydrogel or modified hydrogel according to the invention for use in: lab-on-a-chip systems, microscopy and microarray substrates, microfluidic or sampling, separation, purification, analytical tools.

According to a further aspect of the invention there is provided a kit comprising: i) a hydrogel or modified hydrogel according to the invention; ii) a preparation comprising one or more biocompatible polymers/monomers; iii) cross-linking agent[s].

In a preferred embodiment of the invention said biocompatible polymers/monomers cross-linkable.

In a preferred embodiment of the invention said cross-linkable polymer is selected from the group consisting of: methacrylate, methylmethacrylate, poly methyl methacrylate (PMMA), poly-hydroxyethyl methacrylate (pHEMA), pHEMA-poly ethylene glycol methacrylate or pHEMA-PEGMEM.

Throughout the description and claims of this specification, the words “comprise” and “contain” and variations of the words, for example “comprising” and “comprises”, means “including but not limited to”, and is not intended to (and does not) exclude other moieties, additives, components, integers or steps. “Consisting essentially” means having the essential integers but including integers which do not materially affect the function of the essential integers.

Throughout the description and claims of this specification, the singular encompasses the plural unless the context otherwise requires. In particular, where the indefinite article is used, the specification is to be understood as contemplating plurality as well as singularity, unless the context requires otherwise.

Features, integers, characteristics, compounds, chemical moieties or groups described in conjunction with a particular aspect, embodiment or example of the invention are to be understood to be applicable to any other aspect, embodiment or example described herein unless incompatible therewith.

An embodiment of the invention will now be described by example only and with reference to the following figures:

Figure 1: illustrates a schematic for fabrication of template assisted triple helical polypeptides;

Figure 2: illustrates a schematic for self-assembling via hetero-association of triple helix; Figure 3: illustrates a schematic model of scaffold functionalization;

Figure 4: illustrates synthesis of CMP-PEG self-supporting hydrogel;

Figure 5: illustrates hydrogel supported the growth and proliferation of human corneal epithelial cells and has shown significantly higher enzymatic stability compared with EDC/NHS cross linked human recombinant collagen hydrogels;

Figure 6 illustrates (a) collagen-MPC hydrogel; (b) collagen-MPC hydrogel with central poly methyl methacrylate core serving as an optic;

Figure 7 illustrates comeal epithelial cell culture on the MPC-MMA surface (images taken after 3 days of culture);

Figure 8 illustrates fluorescence micrograph showing visualization of a biotin-terminated pattern;

Figure 9 illustrates a) Human fibronectin (traces of BSA-TR®) patterns on MPC collagen hydrogel: the line width 30 pm, space in-between 60 pm. b) Corneal epithelium cells on 30 pm-wide line patterns on MPC collagen hydrogel; c) Human fibronectin (trace of BSA-TR®) patterns on MPC collagen, the line width 200 pm, spacing 200 pm. d) Corneal epithelium cells on 200 pm line patterns on MPC collagen;

Figure 10 Atomic force microscopy (AFM) analysis of a collagen film (supported on a Si wafer) modified with a layer of PEG hydrogel. A) Section analysis of an AFM topography image of an unmodified collagen film spin-coated on Si wafer. B) The same sample after PEG hydrogel photografting. C) Surface roughness analysis of unmodified collagen film, like in A). D) Surface roughness analysis of the PEG-hydrogel coating on a collagen film;

Figure 11 is a photographic image to illustrate a comparison between CMP/CLP-PEG implants with recombinant human collagen (RHC)-MPC implants;

Figure 12 illustrates the 12 month results of RHCIII-MPC versus CLP-PEG corneas, showing similar performance in epithelial overgrowth and in growth of nerves and stromal cells. Both regenerated corneas show comparable feasters to the control, unoperated healthy cornea; and

Figure 13 illustrates SDS-PAGE profiles of pepsin soluble proteins extracted from the implant region of operated corneas (O), after separation on a 3-8% gradient Tris-acetate gel. Controls comprise identically processed proteins from the unoperated control cornea (C), porcine collagen type 1, pepsin standard, pepsin digest from rat cornea (+) pepsin digest from an unused rhCNI implant (-). This shows that both RHCIII-MPC implanted eyes (animals 1-4) and CLP-PEG implanted eyes (animals 5-8) were remodelled to show type I and type V collagen instead of the initial type III collagen.

Materials & Methods 8-Arm PEG-Maleimide (Mn = 41800) was purchased from Creative PEGWorks, NC, US. 8-Arm PEG-Thiol (Mn = 10000) was purchased from JenKem Technology, Beijing, China. N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide (EDC), N-hydroxysuccinimide (NHS).

Synthesis of CMP A 38 amino acid long CMP peptide, CG- (Pro-Lys-Gly)4(Pro-Hyp-Gly)4(Asp-Hyp-Gly)4, was synthesized on a Symphony automated peptide synthesizer (Protein Technologies Inc., Tucson, AZ, U.S.A.) using standard fluorenylmethoxycarbonyl (Fmoc) chemistry. HCTU (ChemPep Inc., Wellington, FL, USA) was used as the activating reagent. The 0.1 mmol scale synthesis used Fmoc-Gly-PEG-PS resin (Applied Biosystems, Life Technologies Europe BV, Sweden) and five-fold excess of amino acids in each coupling. The resultig peptides were cleaved from the resin by treatment with a mixture of trifluoroacetic acid (TFA), water and triisopropylsilane (TIS) (95:2.5:2.5 v/v; 10 mL per gram of polymer) for 2h at ambient temperature. They were then filtered and the TFA was evaporated. The CMP was then precipitated by the addition of cold diethyl ether, centrifuged and lyophilized. Purification was done using reversed-phase HPLC on a semi-preparative C-18 column (Grace Vydac, Helsingborg, Sweden) and peptide identity was confirmed by their MALDI-TOF spectra (M+1, 3517).

Synthesis of PEG-CMP

Solution of CMP in water and solution of 8-Arm PEG-Maleimide in DMSO was mixed at the molar ratio of PEG-Maleimide : CMP=1:3. After 4 days of continuous stirring at room temp, the mixture was dialyzed through a dialysis membrane (12-14,000 molecular weight, Spectrum Laboratories, Inc., CA, US). After dialysis the solution was lyophilized to get solid PEG-CMP.

Molar composition of PEG:CMP is 1:8 in this example.Please note that this composition will change according to the number of functional arms present on the scaffold. In above example, since we have used 8-arm PEG, we can attach only 8 CMP peptide units to one PEG (template)

Fabrication of CMP hydrogels /implants T-piece mixing system was used to make hydrogel with PEG-CMP, which was previously described [23]. For making the hydrogel 500mg of 12% (w/w) PEG-CMP was taken into a 2ml glass syringes. Calculated volumes of EDC (5% w/v) and NHS (10% w/v) in water were added. Molar equivalent ratio of PEG-CMP-NH2: EDC was 1:0.4 and EDC: NHS was 1:1. All addition followed by thorough mixing.

Fabrication of RHCIII and RHCIII-MPC hydrogels/implants

Two control hydrogels were made; one from 18% human recombinant collagen type III (Fibrogen) (RHC-III) and another one was from RHC-III with 2-methacryloyloxyethyl phosphorylcholine (MPC) (RHCIII-MPC). RHCIII-MPC was made by following previously published procedure [23], 500mg of 18% (w/v) RHC-III was buffered with 150ΠΙ of 0.625M MES buffer and mixed with calculated volume of MPC and PEGDA solution at 4°C. The Collagen:MPC ratio was 2:1 (w/w) and the MPC: PEGDA ratio was 3:1 (w/w). Calculated volume of APS (4% w/v) and TEMED (2% v/v) in MES were added in the Collagen-MPC-PEGDA solution. The ratio of MPC: APS was 1: 0.3 (w/w) and the ratio of APS: TEMED was 1:0.77 (w/w). Calculated volumes of EDC (5% w/v) and NHS (10% w/v) in MES were added. Molar equivalent ratio of Collagen-NH2: EDC was 1:0.4 and EDC: NHS was 1:1.

For RHC-III hydrogel, only NHS and EDC were added following above-mentioned procedure.

For all hydrogels, after adding EDC the final mixed solution was immediately dispensed onto a glass slide and pressed with another glass slides by keeping spacer of 500nm or appropriate size as needed. The moulded solution was kept at 100% humidity. After demoulding, hydrogels were washed with sterilized phosphate buffered saline (PBS). For getting cornea shape construct, terminal mixed collagen/PEG-CMP solution were casted into cornea shape moulds.

Our approach is 3-fold. 1) to use the base template (A) which is stable against enzymatic degradation and supports the cellular ingrowth promoting regeneration 2) modify homogeneously the central core with desired dimensions using synthetic polymer blocking the vessel ingrowth. 3) Surface modification of either the whole implant or central core with polymer/bioactive moieties for desired cellular response.

Base Template

The base template is a bio- and immune compatible, biointeractive material that can be used as a scaffold for stimulating in-growth of host cells (stem or progenitor cells) to differentiate and reform a healthy version of the original tissue. As such we have fabricated base templates based on ECM macromolecules that have been responsible for the original development of the cornea The base template will be one of the following. 1) CMP hydrogels

2) RHCIII / Procine collagen crosslinked with EDC NHS 3) RHCIII +MPC hydrogels.

Central Polymer (Optical) Core

This is the “core” of the invention, the ability to convert a regular corneal implant into a prosthesis as indicated by the patient’s condition. Essentially an acrylated monomer solution with UV initiator Irgacure 2959 is added at the center of hydrogel and allow it to diffuse in (over 15 min) hydrogels. The 15 min time interval allows for conversion of an implant from a regenerative implant to prosthesis during surgery. Gels were exposed to UV-A at 360 nm for 15 mins to form an optical core. CMP-HEMA+PEGMEM prosthesis 12% CMP hydrogels were made using EDC-NHS chemistry. Photoinitiator (Irgacure 2959) was dissolved in 1mL of HEMA and heated up to 60°C to dissolve the Photoinitiator in HEMA solution. Similarly 2wt% of Photoinitiator (Irgacure 2959) solution in PEGMEM was also made. 10μΙ_ of HEMA containing Photoinitiator along with 10μΙ_ of PEGMEM (1:1 volume ratio)was dropped in the center of CMP hydrogels and allowed to diffuse inside the hydrogels for 15mins (Final cone. Of PI - 2 wt%).

After 15 mins, the sample was exposed to UV 365nm for 15 mins. After exposing it to UV, the samples were stored in room temperature for 10 mins to give some curing time of polymers and immediately washed with distilled water to remove the unreacted reactants. CMP- HEMA+MPC prosthesis 12% CMP hydrogels were made using EDC-NHS chemistry. 2wt% of Photoinitiator (Irgacure 2959) was dissolved in 1ml_ of HEMA and heated up to 60°C to dissolve the Photoinitiator in HEMA solution. Similarly 10wt% of MPC solution was made in distilled water. 20μΙ_ of HEMA containing Photoinitiator with 5μΙ_ of MPC was dropped in the center of CMP hydrogels and allowed to diffuse inside the hydrogels for 15mins (Final cone, of Photoinitiator-1.6 wt%). After 15 mins, the sample was exposed to UV 365nm for 15 mins. After exposing it to UV, the samples were stored in room temperature for 10 mins to give some curing time of polymers and immediately washed with distilled water to remove the unreacted reactants. Example 1

After CMP synthesis CMP is mixed with the same or different templating monomers such as for example PEG-Maleimide to assist the assembly of triple helical polypeptides (Figure 1 and 2). CMP can be further functionalised with different biomolecules or markers (Figure 3). The hydrogels comprising the CMP peptide are self-supporting and robust to retain shape (Figure 4). Hydrogels can be modified by adding one or more additional polymers forming a core (Figure 6)

Example 2

Hydrogels comprising the CMP are stable in the presence of collagenase. As shown in figure 5A) the residual weight of the CMP comprising hydrogel is reduced by approximately 10% in the presence of collagenase whereas hydrogels comprising collagen or recombinant human collagen (3H, 3H-MPC, Fibrinogen, Fibrinogen-MPC, Theracol, Theracol-IKVAV orTheracol-YIGSR) show significant digestion after 22h or 50h respectively. CMP comprising hydrogels are highly biocompatible and support cell adhesion and growth. Figure 5B shows coverage by corneal epithelial cells in vitro. The ability to support cell adhesion and growth is retained even after exposure to enzyme.

Example 3

Cell growth on the core material such as MPC-MMA one week after seeding is poor preventing cell and vessel ingrowth and allowing unhindered light transmission. Hydrogels comprising collagen-MPC and grown for one week show cell overgrowth similar to cells cultured for one week in tissue culture plates (Figure 7).

Example 4

The CMP-comprising hydrogels can be easily patterned such as a for example a biotin-terminated pattern using photolithography or imprinting (Figure 8 and 10). The patterned surfaces enable cell growth and/or adhesion. Figure 9 shows fluorescence microscopy of images obtained from the surface of RHCIII-MPC hydrogels patterned with 30 pm (top) and 200 pm stripes (bottom) visualized by staining with an anti-fibronectin antibody [in red]. The corresponding images show corneal epithelial cells growing along the fibronectin stripes. These cells were exposed to a live-dead stain and are mainly green with only 1-2 red cells, showing that majority of cells are viable on the patterned stripes(Figure 9)

Example 5

The comparison of pig corneas comprising RHCIII-MPC versus CLP-PEG at 12 months post-operation shows a similar epithelial overgrowth and in-growth of nerves and stromal cells (Figure 11). Both regenerated corneas showed comparable features to the control, unoperated healthy cornea also shown in the amounts of collagen recovered from the regenerated neo-corneas after RHCIII-MPC and CLP-PEG implantation versus control, unoperated healthy corneas (Figure 11 and 12).

Example 6 SDS-PAGE profiles of pepsin soluble proteins extracted from the implant region of operated corneas (O), after separation on a 3-8% gradient Tris-acetate gel. Controls comprise identically processed proteins from the unoperated control cornea (C), porcine collagen type 1, pepsin standard, pepsin digest from rat cornea (+) pepsin digest from an unused rhCNI implant (-). This shows that both RHCIII-MPC implanted eyes (animals 1-4) and CLP-PEG implanted eyes (animals 5-8) were remodelled to show type I and type V collagen instead of the initial type III collagen (Figure 13).

Claims

Claims 1 A peptide comprising an amino acid sequence wherein said peptide comprises at least four amino acid motifs represented in the following formula:

(Xaa^ Xaa2) Xaa! is a thiol containing amino acid and Xaa2 is a glycine amino acid wherein w comprises at least one repeat of a first amino acid motif, (Xaa3- Xaa4 -Xaa5)x is a second amino acid motif wherein Xaa3 is proline, Xaa4 is lysine, Xaa5 is glycine wherein x comprises 1-4 repeats of said second amino acid motif, (Xaa6-Xaa7-Xaa8)y is a third amino acid motif wherein Xaa6 is proline, Xaa7 is hydroxyproline, Xaa8 is glycine wherein y comprises 1-4 repeats of said third amino acid motif; and (Xaag-Xaa^-Xaan) is a fourth amino acid motif wherein Xaa9 is aspartic acid or glutamic acid, Xaa10 is hydroxyproline, Xaa^ is glycine wherein z comprises 1-4 repeats of said fourth amino acid motif.
2. The peptide according to claim 1, wherein said thiol containing amino acid is cysteine. 3 The peptide according to claim 1 or 2 wherein said first amino acid motif comprises more than 1 repeat; preferably said peptide comprises at least 1, 2, 3 or 4 repeats of said first amino acid motif.
4. The peptide according to any one of claims 1 to 3, wherein said peptide comprises at least, 1, 2, 3 or 4 repeats of said second amino acid motif.
5. The peptide according to any one of claims 1 to 4, wherein said peptide comprises at least 1, 2, 3 or 4 repeats of said third amino acid motif.
6. The peptide according to any one of claims 1 to 5 wherein said peptide comprises at least 1, 2, 3 or 4 repeats of said fourth amino acid motif.
7. The peptide according to claim 1 or 2 wherein said peptide comprises the amino acid sequence

wherein Xaai is a natural or modified thiol containing amino acid.
8. The peptide according to claim 1 or 2 wherein said peptide comprises the amino acid sequence

wherein said peptide is a collagen mimetic peptide.
9. The peptide according to any one of claims 1 to 8 wherein said peptide is at least 11 amino acids in length; preferably said peptide comprises 11 to 38 amino acids.
10. The peptide according to any one of claims 1 to 9 wherein said peptide is derivatized by chemical modification to provide one or more reactive groups.
11. The peptide according to claim 10 wherein said peptide is modified by addition of one or more functional groups selected from the group consisting of: thiol, methyacrylate or acrylate functional groups.
12. The peptide according to claim 10 or 11 wherein said modified peptide comprises polyethylene glycol; preferably polyethylene glycol-maleimide or polyethylene glycol diacrylate or polyethylene glycol methacrylate.
13. The peptide according to claim 12 wherein said polyethylene glycol-maleimide is at least 2, 4, 6 or 8 arm polyethylene glycol-maleimide.
14. A hydrogel comprising: a plurality of modified collagen mimetic peptides according to any one of claims 10 to 13 chemically cross linked into a network.
15. The hydrogel according to claim 14 wherein said modified collagen mimetic peptide is activated via N-ethyl-N'-[3-dimethylaminopropyl] carbodiimide/N-hydroxy succinimide (EDC/NHS).
16. The hydrogel according to claim 14 or 15 wherein said hydrogel comprises one or more natural or synthetic biopolymers chemically cross linked to said network.
17. The hydrogel according to claim 16 wherein said natural polymer is a collagen.
18. The hydrogel according to claim 17 wherein said collagen is selected from the group consisting of: collagen I, collagen II, collagen III, collagen IV or collagen V, or mixtures thereof.
19. The hydrogel according to any one of claims 16 to 18 wherein said natural polymer is selected from the group consisting of: fibrin, cell-interactive proteins, for example laminin, fibronectin, hyaluronic acid, chitosan, collagen mimetic peptides of different sequence to the collagen mimetic peptide according to any one of claims 1 to 13.
20. The hydrogel according to any one of claims 16 to 19 wherein said synthetic polymer is selected from the group consisting of: functionalized polyethylene glycol (PEG), polyvinyl alcohol (PVA), polyethylene glycol-diacrylate (PEGDA), PEG methacrylate (PEGMA), poly(hydroxyethyl methacrylate) (pHEMA), polyethylene glycol methyl ether methacrylate (PEGMEM), poly(pentaerythritol triacrylate) or poly(N-isopropylacryl amide) (PNIPAAm).
21. The hydrogel according to claim 20 wherein said network comprises a photo initiator.
22. The hydrogel according to claim 21 wherein said network is photo-crosslinked.
23. The hydrogel according to any one of claims 16 to 22 wherein the elastic modulus of the hydrogel is at least 1 MPa or at least 5 MPa.
24. The hydrogel according to any one of claims 16 to 23 wherein said hydrogel is substantially transparent and allows a light transmission of at least 80 or 90% of light in the range of 400-700nm.
25. The hydrogel according to any one of claims 16 to 24 wherein the total concentration of polymers in the hydrogel is at least 2 weight% or more.
26. A method of manufacturing a hydrogel comprising the steps: i) providing a preparation comprising a modified peptide according to any one of claims 10 to 13; ii) providing a preparation comprising one or more biocompatible polymers and/or monomers wherein said polymers are provided with one or more functional groups; iii) combining preparations i) and ii) to provide a reaction mixture and contacting said reaction mixture with a crosslinking agent. iv) incubating the reaction mixture to crosslink said modified peptide with one or more biocompatible polymers and/or monomers to provide a cross-linked network.
27. The method according to claim 26 wherein said functional groups are selected from the group consisting of: thiol, acrylate and/or methacrylate, or synthetic monomers having thiol, acrylate and/or methacrylate functional groups.
28. The method according to claim 26 or 27 wherein said cross-linking agent is a chemical cross-linker.
29. The method according to claim 26 or 27 wherein said cross-linking agent is a photoinitiator and UV radiation.
30. A hydrogel according to any one of claims 14 to 25 wherein said hydrogel is modified to provide a uniformly functionalized or patterned surface to which cells and/or biocompatible polymers/monomers adhere to modulate cell contact.
31. A method for the modification of a hydrogel comprising: i) providing a hydrogel according to any one of claims 14 to 25; ii) applying to the surface of said hydrogel one or more monomeric or polymeric agents to modify the surface of the hydrogel; and/or iii) treating the applied surface compounds by micro-contact printing or photolithography or inkjet printing, or any other fabrication technique in order to create a pattern of said surface compounds.
32. The method according to claim 31 wherein said agents are selected from the group consisting of: polyethylene glycol (PEG)-acrylate polymer/monomers, PEG-methacrylate polymer/monomers, other acrylate, methacrylate, carboxyl, amino, amide, epoxide, hydroxyl, cyano, nitride, sulfonamido, acetylenyl, alkene, esters like imidoesters( N-hydroxysuccinimide ester) or pentafluorphenol ester or other, azide, thiol, maleimide, functionalized PEG derivatives, any bifunctional or multifunctional compounds, drugs, bioactive substances, biological molecules including proteins or peptides to said surface.
33. The method according to claim 31 or 32 wherein said agent is a cell adhesion or cell modulating protein.
34. The method according to claim 33 wherein said agent is selected from the group consisting of: fibronectin, laminin, vitronectin or peptide motifs derived from these proteins.
35. The method according to any one of claims 31 to 34 wherein the pattern is in the shape of lines or dots or any other geometrical figures.
36. The method according to claim 35 wherein the lines, dots or geometrical figures have a width or diameter of at least 10 nm or more.
37. The method according to claim 35 wherein the lines, dots orgeometrical figures have a width or diameter from 1 pm or more.
38. A modified hydrogel obtained or obtainable by the method according to any one of claims 31 to 37.
39. The hydrogel or modified hydrogel according to any one of claims 13 to 24 or 29 comprises at least one cell type.
40. The hydrogel according to claim 39 wherein said cell type is a mammalian cell; preferably a human cell.
41. The hydrogel according to claim 40 wherein said cell type is a stem cell.
42. The hydrogel according to claim 41 wherein said stem cell is a corneal stem cell or progenitor cell.
43. The hydrogel according to claim 41 wherein said stem cell is a corneal endothelial cell or progenitor cell.
44. The hydrogel according to claim 40 wherein said cell is an epithelial cell or a mesenchymal cell.
45. A corneal implant comprising a hydrogel according to any one of claims 14 to 25 or 30 or any one of claims 38 to 44.
46. The corneal implant according to claim 45 wherein said corneal implant comprises a matrix part comprising a hydrogel according to the invention and a core part wherein the core part is substantially centrally located in the matrix part and comprises polymerized olefinic or UV polymerizable monomers, or a mixture thereof, the hydrogel of the matrix material and one or more antifouling polymers/agents characterised in that the core part is substantially transparent and free of cells/vessels.
47. The corneal implant according to claim 46 wherein the core material comprises: HEMA and PEGMEM or HEMA and MPC.
48. The corneal implant according to claim 46 or 47 wherein said polymerized olefinic or UV polymerizable monomers are selected from the group: methyacrylate, methylmethacrylate, poly methyl methacrylate (PMMA), poly-hydroxyethyl methacrylate (pHEMA), pHEMA-poly ethylene glycol methacrylate or pHEMA-PEGMEM.
49. The corneal implant according to any one of claims 45 to 47 wherein the surface of the matrix material and/or the core material is modified and/or patterned with PEG-MA.
50. The corneal implant according to any one of claims 45 to 49 wherein the surface of the matrix material and/or the core material is activated using NHS/EDC.
51. The corneal implant according to any one of claims 45 to 50 wherein the surface of the matrix material and/or the core material is modified and/or patterned with N-hydroxysulfosuccinimide (NHS), polypeptides such as YIGSR, IKVAV, RGD, ECM proteins, fibrinectin derived peptides, combinations of synergestic peptides, DGEA peptide from collagen, antibodies, glycosaminoglucans, motifs from growth factors, or pharmaceutically active substances.
52. The corneal implant according to any one of claims 45 to 51 wherein the matrix and/or core material comprises at least the modified collagen mimetic peptide according to any one of claims 9 to 12 modified with polyethylene glycol wherein the PEG has 2, 4, 6 or 8 arms.
53. The corneal implant according to any one of claims 45 to 52 wherein said core material comprises HEMA and/or PEGMEM, or HEMA and/or MPC.
54. A method for the manufacture of an implant according to any one of claims 45 to 53 comprising: i) providing a matrix polymer and a cross-linker in a suitable solvent; ii) cross-linking the matrix polymer to form the matrix material; iii) providing core olefinic monomers or UV polymerizable monomers or polypeptides or a mixtures thereof, and an initiator, optionally in a suitable solvent; iv) adding the core olefinic monomers or UV polymerizable monomers or polypeptides, or a mixtures thereof and the initiator to the surface or the body of the matrix material; and v) allowing the core olefinic monomers to polymerize for a suitable period of time.
55. The method according to claim 54 wherein the olefinic based material is left to diffuse after addition in at least 15 minutes.
56. The method according to claim 54 or 55 wherein the curing of said core is obtained during UV radiation using a mask.
57. A corneal implant according to any one of claims 45 to 53 for use in the repair or replacement of diseased or damaged corneal tissue.
58. The corneal implant according to claim 57 wherein said disease is selected from the group consisting of: Fuchs' Dystrophy, iridocorneal endothelial syndrome, keratoconus, lattice dystrophy, ocular herpes infections, trachoma are examples.
59. The corneal implant according to claim 58 wherein said damage is chemical damage, physical injury during sport or during military conflict.
60. A surgical method for the repair or replacement of diseased or damaged corneal tissue in a subject in need of corneal repair or damage comprising: i) providing a corneal implant according to any one of claims 45 to 53; ii) attaching the corneal implant to the eye of said subject; and optionally iii) providing a protective covering to the repaired eye to facilitate healing.
61. A hydrogel or modified hydrogel according to any one of claims of claims 14 to 25 or 30, or any one of claims 38 to 44 for use in cell and tissue culture.
62. A hydrogel or modified hydrogel of claims 14 to 25 or 30 or any one of claims 38 to 44 for use in: lab-on-a-chip systems, microscopy and microarray substrates, microfluidic or sampling, separation, purification, analytical tools.
63. A kit comprising: i) a hydrogel or modified hydrogel of claims 14 to 25 or 30 or any one of claims 38 to 44; ii) a preparation comprising one or more biocompatible polymers/monomers; Hi) cross-linking agent[s],
64. The kit according to claim 63 wherein said biocompatible polymers/monomers cross-linkable.
65. The kit according to claim 63 or 64 wherein said cross-linkable polymer is selected from the group consisting of: methacrylate, methylmethacrylate, poly methyl methacrylate (PMMA), poly-hydroxyethyl methacrylate (pHEMA), pHEMA-poly ethylene glycol methacrylate or pHEMA-PEGMEM. Claims 1 A peptide comprising an amino acid sequence wherein said peptide consists of the amino acid sequence: Cys-Gly - (Pro-Lys-GlyMPro-Hyp-GlyMXaag-Hyp-Gly)* wherein Xaa9is aspartic acid or glutamic acid.
2. The peptide according to claim 1 wherein said peptide comprises polyethylene glycol; preferably polyethylene glycol-maleimide or polyethylene glycol 4iacrylate or polyethylene glycol methacrylate.
3. The peptide according to claim 2 wherein said polyethylene glycol-maleimide is at least 2, 4, 6 or 8 arm polyethylene glycol-maleimide;
4. A hydrogel comprising: a plurality of modified collagen mimetic peptides according to claim 2 to 3 chemically cross linked into a network.
5. The hydrogel according to claim 4 wherein said modified collagen mimetic peptide is activated via N-ethyl-N'-[3-dimethylaminopropyl] carbodiimide/N-hydroxy succinimide (EDC/NHS).
6. The hydrogel according to claim 4 or 5 wherein said hydrogel comprises polyethylene glycol methyl ether methacrylate (PEGMEM) and hydroxyethyl methacrylate (HEMA).
7. The hydrogel according to any one of claims 4 to 6 wherein said hydrogel is substantially transparent and allows a light transmission of at least 80 or 90% of light in the range of 400-700nm.
8. The hydrogel according to any one of claims 4 to 7 wherein the total concentration of polymers in the hydrogel is at least 2 weight% or more.
9. A method of manufacturing a hydrogel comprising the steps: i) providing a preparation comprising a modified peptides according to claim 2 or 3; and ii) adding to said preparation a mixture of N-ethyl-N'-[3-dimethylaminopropyl] carbodiimide/N-hydroxy succinimide (EDC/NHS) to crosslink said modified peptides to form a hydrogel.
10. A hydrogel according to any one of claims 4 to 8 wherein said hydrogel is modified to provide a uniformly functionalized or patterned surface to which cells and/or biocompatible polymers/monomers adhere to modulate cell contact.
11. A method for the modification of a hydrogel comprising: i) providing a hydrogel according to any one of claims 4 to 8; ii) applying to the surface of said hydrogel one or more monomeric or polymeric agents to modify the surface of the hydrogel; and/or iii) treating the applied surface compounds by micro-contact printing or photolithography or inkjet printing, or any other fabrication technique in order to create a pattern of said surface compounds.
12. The method according to claim 11 wherein said agents are selected from the group consisting of: polyethylene glycol (PEG)-acrylate polymer/monomers, PEG-methacrylate polymer/monomers, other acrylate, methacrylate, carboxyl, amino, amide, epoxide, hydroxyl, cyano, nitride, sulfonamido, acetylenyl, alkene, esters like imidoesters( N-hydroxysuccinimide ester) or pentafluorphenol ester or other, azide, thiol, maleimide, functionalized PEG derivatives, any bifunctional or multifunctional compounds, drugs, bioactive substances, biological molecules including proteins or peptides to said surface.
13. The method according to claim 11 or 12 wherein said agent is a cell adhesion or cell modulating protein.
14. The method according to claim 13 wherein said agent is selected from the group consisting of: fibronectin, laminin, vitronectin or peptide motifs derived from these proteins.
15. The method according to any one of claims 11 to 14 wherein the pattern is in the shape of lines or dots or any other geometrical figures.
16. The method according to claim 15 wherein the lines, dots or geometrical figures have a width or diameter of at least 10 nm or more.
17. The method according to claim 15 wherein the lines, dots or geometrical figures have a width or diameter from 1 pm or more.
18. The hydrogel or modified hydrogel according to any one of claims 4 to 8 or 10 comprises at least one cell type.
19. The hydrogel according to claim 18 wherein said cell type is a mammalian cell.
20. The hydrogel according to claim 19 wherein said mammalian cell is a human cell.
21. The hydrogel according to claim 19 or 20 wherein said cell type is a stem cell.
22. The hydrogel according to claim 21 wherein said stem cell is a corneal stem cell or progenitor cell.
23. The hydrogel according to claim 21 wherein said stem cell is a corneal endothelial cell or progenitor cell.
24. The hydrogel according to claim 19 or 20 wherein said cell is an epithelial cell or a mesenchymal cell.
25. A corneal implant comprising a hydrogel according to any one of claims 4 to 8 or 10.
26. The corneal implant according to claim 25 wherein said corneal implant comprises a matrix part comprising a hydrogel according to any one of claims 4 to 8 or 10 and a core part wherein the core part is substantially centrally located in the matrix part and comprises polymerized HEMA and PEGMEM, the hydrogel of the matrix material and one or more antifouling polymers/agents characterised in that the core part is substantially transparent and free of cells/vessels.
27. The corneal implant according to claim 25 or 26 wherein the surface of the matrix material and/or the core material is modified and/or patterned with PEG-MA.
28. The corneal implant according to any one of claims 25 to 27 wherein the surface of the matrix material and/or the core material is modified and/or patterned with N-hydroxysulfosuccinimide (NHS), polypeptides such as YIGSR, IKVAV, RGD, ECM proteins, fibrinectin derived peptides, combinations of synergestic peptides, DGEA peptide from collagen, antibodies, glycosaminoglucans, motifs from growth factors, or pharmaceutically active substances.
29. The corneal implant according to any one of claims 25 to 28 wherein the matrix and/or core material comprises at least the modified collagen mimetic peptide according to any one of claims 2 to 5 modified with polyethylene glycol wherein the PEG has 2, 4, 6 or 8 arms.
30. A method for the manufacture of an implant according to any one of claims 25 to 29 comprising: i) providing a hydrogel according any one of claims 4 to 10; ii) providing a mixture comprising HEMA and PEGMEM and an photo-initiator in a suitable solvent; iii) adding the mixture in (ii) to the surface or the body of the hydrogel; and iv) allowing the core comprising HEMA and PEGMEM to polymerize for a suitable period of time.
31. The method according to claim 30 wherein the HEMA and PEGMEM is left to diffuse after addition in at least 15 minutes.
32. The method according to claim 30 or 31 wherein the curing of said core is obtained during UV radiation using a mask.
33. A corneal implant according to any one of claims 11 to 16 for use in the repair or replacement of diseased or damaged corneal tissue.
34. The corneal implant according to claim 33 wherein said disease is selected from the group consisting of: Fuchs' Dystrophy, iridocorneal endothelial syndrome, keratoconus, lattice dystrophy, ocular herpes infections, trachoma are examples.
35. The corneal implant according to claim 33 wherein said damage is chemical damage, physical injury during sport or during military conflict.
36. A hydrogel or modified hydrogel according to any one of claims 4 to 8 or 10 for use in cell and tissue culture.
37. A hydrogel or modified hydrogel of claims 4 to 8 or 10 for use in: lab-on-a-chip systems, microscopy and microarray substrates, microfluidic or sampling, separation, purification, analytical tools.
38. A kit comprising: i) a hydrogel or modified hydrogel of claims 4 to 8 or 10; and ii) a preparation comprising HEMA and PEGMEM; iii) photo-initiator agent[s].