GB2521893A - Cell migration assay - Google Patents

Cell migration assay Download PDF

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Publication number
GB2521893A
GB2521893A GB1416623.5A GB201416623A GB2521893A GB 2521893 A GB2521893 A GB 2521893A GB 201416623 A GB201416623 A GB 201416623A GB 2521893 A GB2521893 A GB 2521893A
Authority
GB
United Kingdom
Prior art keywords
microchannel
fluid sample
cell
cell migration
access port
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB1416623.5A
Other versions
GB201416623D0 (en
Inventor
David Neville Steele
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BIOCOLOR Ltd
Original Assignee
BIOCOLOR Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BIOCOLOR Ltd filed Critical BIOCOLOR Ltd
Publication of GB201416623D0 publication Critical patent/GB201416623D0/en
Publication of GB2521893A publication Critical patent/GB2521893A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/028—Graduation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G01N2015/1027—

Abstract

A cell migration measurement device comprising a substrate member with: • at least one access port in a surface of the substrate adapted to receive a fluid sample; at least one exit port (5) adapted to output fluid sample from the device; • at least one internal surface of the device defining at least one microchannel in the substrate extending from and connected to the access port and the exit port (5) of the device for flow of the fluid sample through the microchannel from the access port to the exit port (5), a cell receiving portion comprising multiple hopper shaped cell receiving portions (3) provided in fluid connection with the microchannel, wherein each of the cell receiving portions (3) provide cells provided in the fluid sample to a constriction portion (7) which is in fluid connection with a cell measuring microchannel (4).
GB1416623.5A 2012-03-20 2013-03-20 Cell migration assay Withdrawn GB2521893A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1204848.4A GB201204848D0 (en) 2012-03-20 2012-03-20 Cell migration assay
PCT/GB2013/050718 WO2013140164A1 (en) 2012-03-20 2013-03-20 Cell migration assay

Publications (2)

Publication Number Publication Date
GB201416623D0 GB201416623D0 (en) 2014-11-05
GB2521893A true GB2521893A (en) 2015-07-08

Family

ID=46052207

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB1204848.4A Ceased GB201204848D0 (en) 2012-03-20 2012-03-20 Cell migration assay
GB1416623.5A Withdrawn GB2521893A (en) 2012-03-20 2013-03-20 Cell migration assay

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB1204848.4A Ceased GB201204848D0 (en) 2012-03-20 2012-03-20 Cell migration assay

Country Status (2)

Country Link
GB (2) GB201204848D0 (en)
WO (1) WO2013140164A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1340810A1 (en) * 2000-12-07 2003-09-03 Effector Cell Institute Apparatus for treating sample in microamount
US20060121600A1 (en) * 2003-04-09 2006-06-08 Shiro Kanegasaki Apparatus for detecting cell chemotaxis
US20090155877A1 (en) * 2004-07-06 2009-06-18 Agency For Science Technology And Research Biochip for sorting and lysing biological samples
US20120064505A1 (en) * 2010-03-22 2012-03-15 Massachusetts Institute Of Technology Measurement of material properties and related methods and compositions

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1340810A1 (en) * 2000-12-07 2003-09-03 Effector Cell Institute Apparatus for treating sample in microamount
US20060121600A1 (en) * 2003-04-09 2006-06-08 Shiro Kanegasaki Apparatus for detecting cell chemotaxis
US20090155877A1 (en) * 2004-07-06 2009-06-18 Agency For Science Technology And Research Biochip for sorting and lysing biological samples
US20120064505A1 (en) * 2010-03-22 2012-03-15 Massachusetts Institute Of Technology Measurement of material properties and related methods and compositions

Also Published As

Publication number Publication date
WO2013140164A1 (en) 2013-09-26
GB201416623D0 (en) 2014-11-05
GB201204848D0 (en) 2012-05-02

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)