GB2519910A - Methods of isolating nucleic acids under reduced degradation condition - Google Patents

Methods of isolating nucleic acids under reduced degradation condition Download PDF

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Publication number
GB2519910A
GB2519910A GB201503908A GB201503908A GB2519910A GB 2519910 A GB2519910 A GB 2519910A GB 201503908 A GB201503908 A GB 201503908A GB 201503908 A GB201503908 A GB 201503908A GB 2519910 A GB2519910 A GB 2519910A
Authority
GB
United Kingdom
Prior art keywords
nucleic acids
methods
under reduced
isolating nucleic
acids under
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB201503908A
Other versions
GB201503908D0 (en
GB2519910B (en
Inventor
John Richard Nelson
Li Zhu
Erin Jean Finehout
Xiaohui Chen
Kashan Ali Shaikh
Christopher Michael Puleo
Patrick Mccoy Spooner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/599,124 external-priority patent/US20140039177A1/en
Application filed by General Electric Co filed Critical General Electric Co
Priority to GB1714375.1A priority Critical patent/GB2552110B/en
Publication of GB201503908D0 publication Critical patent/GB201503908D0/en
Publication of GB2519910A publication Critical patent/GB2519910A/en
Application granted granted Critical
Publication of GB2519910B publication Critical patent/GB2519910B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
GB1503908.4A 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition Expired - Fee Related GB2519910B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1714375.1A GB2552110B (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/599,124 US20140039177A1 (en) 2012-07-31 2012-08-30 Methods of isolating nucleic acids under reduced degradation condition
PCT/US2013/048853 WO2014035547A2 (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition

Publications (3)

Publication Number Publication Date
GB201503908D0 GB201503908D0 (en) 2015-04-22
GB2519910A true GB2519910A (en) 2015-05-06
GB2519910B GB2519910B (en) 2018-05-09

Family

ID=50184574

Family Applications (2)

Application Number Title Priority Date Filing Date
GB1714375.1A Expired - Fee Related GB2552110B (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition
GB1503908.4A Expired - Fee Related GB2519910B (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB1714375.1A Expired - Fee Related GB2552110B (en) 2012-08-30 2013-07-01 Methods of isolating nucleic acids under reduced degradation condition

Country Status (2)

Country Link
GB (2) GB2552110B (en)
WO (1) WO2014035547A2 (en)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6572830B1 (en) * 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6555945B1 (en) * 1999-02-25 2003-04-29 Alliedsignal Inc. Actuators using double-layer charging of high surface area materials
WO2004005553A1 (en) * 2002-07-10 2004-01-15 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
CA2610875A1 (en) * 2005-06-06 2006-12-14 Decision Biomarkers, Inc. Assays based on liquid flow over arrays
US20070021735A1 (en) * 2005-07-15 2007-01-25 Sai Bhavaraju Dual membrane electro-osmotic fluid delivery device
US20110097215A1 (en) * 2009-10-23 2011-04-28 The Government Of The United States Of America, As Represented By The Secretary Of The Navy Flexible Solid-State Pump Constructed of Surface-Modified Glass Fiber Filters and Metal Mesh Electrodes
US20120152743A1 (en) * 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
NOT YET ADVISED *

Also Published As

Publication number Publication date
GB201503908D0 (en) 2015-04-22
GB2552110B (en) 2018-05-23
WO2014035547A2 (en) 2014-03-06
WO2014035547A3 (en) 2015-07-23
GB2552110A (en) 2018-01-10
GB2519910B (en) 2018-05-09
GB201714375D0 (en) 2017-10-25

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20200701