GB2512664B - Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis - Google Patents

Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis

Info

Publication number
GB2512664B
GB2512664B GB1306286.4A GB201306286A GB2512664B GB 2512664 B GB2512664 B GB 2512664B GB 201306286 A GB201306286 A GB 201306286A GB 2512664 B GB2512664 B GB 2512664B
Authority
GB
United Kingdom
Prior art keywords
visualisation
gel electrophoresis
polyacrylamide gel
target molecules
loading buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB1306286.4A
Other versions
GB2512664A (en
GB201306286D0 (en
Inventor
Daniel Brownleader Michael
Purvis Alan
Kugendradas Arumugam
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to GB1306286.4A priority Critical patent/GB2512664B/en
Publication of GB201306286D0 publication Critical patent/GB201306286D0/en
Publication of GB2512664A publication Critical patent/GB2512664A/en
Application granted granted Critical
Publication of GB2512664B publication Critical patent/GB2512664B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
GB1306286.4A 2013-04-05 2013-04-05 Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis Expired - Fee Related GB2512664B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB1306286.4A GB2512664B (en) 2013-04-05 2013-04-05 Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB1306286.4A GB2512664B (en) 2013-04-05 2013-04-05 Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis

Publications (3)

Publication Number Publication Date
GB201306286D0 GB201306286D0 (en) 2013-05-22
GB2512664A GB2512664A (en) 2014-10-08
GB2512664B true GB2512664B (en) 2017-09-13

Family

ID=48483500

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1306286.4A Expired - Fee Related GB2512664B (en) 2013-04-05 2013-04-05 Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis

Country Status (1)

Country Link
GB (1) GB2512664B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138366A2 (en) * 2005-06-16 2006-12-28 New England Biolabs, Inc. Protein markers
US20090035868A1 (en) * 2007-05-18 2009-02-05 Invitrogen Corporation Rapid protein labeling and analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006138366A2 (en) * 2005-06-16 2006-12-28 New England Biolabs, Inc. Protein markers
US20090035868A1 (en) * 2007-05-18 2009-02-05 Invitrogen Corporation Rapid protein labeling and analysis

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Anal. Biochem., Vol.61, 1974, Sun, S. M. & Hall, T. C., "Application of Remazol dye for isolation of...", pp.237-242 *
Bioconjugate Chem., Vol.23, 2012, Jin Woo Park, et al., "Novel cyanine dyes...", pp.350-362 *
Clin. Chem., Vol.29, 1983, Saoji, A. M. et al., "Remazol brilliant blue as a...", pp.42-44 *
Electrophoresis, Vol.23, 2002, Compton, M. M. et al., "Generation of multicoloured...", pp.3262-3265 *

Also Published As

Publication number Publication date
GB2512664A (en) 2014-10-08
GB201306286D0 (en) 2013-05-22

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20141113 AND 20141119

732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20180503 AND 20180509

PCNP Patent ceased through non-payment of renewal fee

Effective date: 20230405