GB2505819B - Method for identifying genes involved in antibiotic resistance and sensitivity in gram-negative bacteria - Google Patents

Method for identifying genes involved in antibiotic resistance and sensitivity in gram-negative bacteria

Info

Publication number
GB2505819B
GB2505819B GB201322084A GB201322084A GB2505819B GB 2505819 B GB2505819 B GB 2505819B GB 201322084 A GB201322084 A GB 201322084A GB 201322084 A GB201322084 A GB 201322084A GB 2505819 B GB2505819 B GB 2505819B
Authority
GB
United Kingdom
Prior art keywords
gram
sensitivity
negative bacteria
antibiotic resistance
genes involved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB201322084A
Other versions
GB201322084D0 (en
GB2505819A (en
Inventor
David Hugh Williams
Arthur Keith Turner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Discuva Ltd
Original Assignee
Discuva Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Discuva Ltd filed Critical Discuva Ltd
Priority to GB201322084A priority Critical patent/GB2505819B/en
Publication of GB201322084D0 publication Critical patent/GB201322084D0/en
Publication of GB2505819A publication Critical patent/GB2505819A/en
Application granted granted Critical
Publication of GB2505819B publication Critical patent/GB2505819B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
GB201322084A 2013-12-13 2013-12-13 Method for identifying genes involved in antibiotic resistance and sensitivity in gram-negative bacteria Active GB2505819B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB201322084A GB2505819B (en) 2013-12-13 2013-12-13 Method for identifying genes involved in antibiotic resistance and sensitivity in gram-negative bacteria

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB201322084A GB2505819B (en) 2013-12-13 2013-12-13 Method for identifying genes involved in antibiotic resistance and sensitivity in gram-negative bacteria

Publications (3)

Publication Number Publication Date
GB201322084D0 GB201322084D0 (en) 2014-01-29
GB2505819A GB2505819A (en) 2014-03-12
GB2505819B true GB2505819B (en) 2014-10-01

Family

ID=50030898

Family Applications (1)

Application Number Title Priority Date Filing Date
GB201322084A Active GB2505819B (en) 2013-12-13 2013-12-13 Method for identifying genes involved in antibiotic resistance and sensitivity in gram-negative bacteria

Country Status (1)

Country Link
GB (1) GB2505819B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201107515D0 (en) 2011-05-05 2011-06-22 Discuva Ltd Method for identifying antibiotic targets
GB201413202D0 (en) * 2014-07-25 2014-09-10 Discuva Ltd Process for producing bacterial mutants
GB201413198D0 (en) * 2014-07-25 2014-09-10 Discuva Ltd Methods For Characterizing Bacterial Mutants
GB201421850D0 (en) * 2014-12-09 2015-01-21 Bactevo Ltd Method for screening for bioactive natural products
GB201507931D0 (en) 2015-05-08 2015-06-24 Discuva Ltd Combination therapies for combating resistance
GB202015512D0 (en) 2020-09-30 2020-11-11 Demuris Ltd Biotechnological methods
CN116042688B (en) * 2023-03-06 2023-06-20 中山大学 Citrus canker high-saturation transposon mutant library and construction method thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Meredith TC, et al.," Harnessing the power of transposon mutagenesis for antibacterial target identification and evolution", Mob. Genet. Elements, vol. 2, no. 4, 2012 , pages 171-178, document available online at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3469428/pdf/mge-2-171.pdf (accessed 3/2/14) *
Wang H, et al., "High-frequency transposition for determining antibacterial mode of action", Nature Chemical Biology, vol. 7, no. 10, 2011, pages 720-729 *

Also Published As

Publication number Publication date
GB201322084D0 (en) 2014-01-29
GB2505819A (en) 2014-03-12

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