GB250230A - Improved manufacture of substances for reducing arterial hypertension - Google Patents

Abstract

250,230. Macdonald, W. J. April 3, 1925, [Convention date]. Hypodermic injections.-Substances for injection to reduce arterial hypertension are prepared by making an extract or liver or related glands, purifying the extract, and fractionally precipitating two active substances therefrom. In an example, calf liver is ground in the presence of chloretone, and extracted with Locke's solution containing a trace of sulphuric acid. The solution is filtered and evaporated to dryness under reduced pressure. The residue is extracted with alcohol and the solution filtered and evaporated to dryness in an air current. The substance so obtained is taken up in physiological salt solution and sterilized by filtration through a Berkefeld filter. Two active substances are separated from the extract by adding an equal volume of alcohol and then gradually adding acetone to precipitate first an oily substance, which is then removed, and finally a solid white substance. The oily substance is centrifuged, decanted off from any solid material present, purified if necessary by re-solution and precipitation, taken up in normal saline and passed through a Berkefeld filter. The solid precipitate obtained on adding more acetone after removal of the oily substance is removed from the solution by filtration, purified if necessary by re-solution and precipitation, dried in an air current, dissolved in normal saline, and the solution passed through a Berkefeld filter. To prevent precipitation on standing, the hydrogen ion concentration of the solution is adjusted by addition of sulphuric acid. The two active substances may be employed separately or mixed together. The Specification as open to inspection under Sect. 91 (3) (a) relates also to the preparation of the active substances from blood, lung, spleen, pancreas, thymus, heart, kidney or muscle. This subject-matter does not appear in the Specification as accepted.