GB2475223A - A Paramyxoviridae family N protein-RNA complex in crystal form - Google Patents

A Paramyxoviridae family N protein-RNA complex in crystal form Download PDF

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Publication number
GB2475223A
GB2475223A GB0916802A GB0916802A GB2475223A GB 2475223 A GB2475223 A GB 2475223A GB 0916802 A GB0916802 A GB 0916802A GB 0916802 A GB0916802 A GB 0916802A GB 2475223 A GB2475223 A GB 2475223A
Authority
GB
United Kingdom
Prior art keywords
protein
crystal form
rna
rna complex
paramyxoviridae family
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0916802A
Other versions
GB0916802D0 (en
Inventor
Felix Augusto Rey
Jean-Francois Eleouet
Rajeevkumar Gir Tawar
Stephane Duquerroy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Institut National de la Recherche Agronomique INRA
Original Assignee
Institut Pasteur de Lille
Institut National de la Recherche Agronomique INRA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille, Institut National de la Recherche Agronomique INRA filed Critical Institut Pasteur de Lille
Priority to GB0916802A priority Critical patent/GB2475223A/en
Publication of GB0916802D0 publication Critical patent/GB0916802D0/en
Priority to PCT/IB2010/002560 priority patent/WO2011036563A2/en
Publication of GB2475223A publication Critical patent/GB2475223A/en
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C07K14/08—RNA viruses
    • C07K14/115—Paramyxoviridae, e.g. parainfluenza virus
    • C07K14/135—Respiratory syncytial virus
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
    • C30B29/54—Organic compounds
    • C30B29/58—Macromolecular compounds
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • C30B7/14—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions the crystallising materials being formed by chemical reactions in the solution
    • G06F19/16—
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18511—Pneumovirus, e.g. human respiratory syncytial virus
    • C12N2760/18522—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Metallurgy (AREA)
  • Materials Engineering (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Physics & Mathematics (AREA)
  • Communicable Diseases (AREA)
  • Pulmonology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The application claims an N-RNA complex in crystal form comprising an N protein (nucleoprotein) of a non-segmeneted negative strand RNA virus of the Paramyxoviridae family such as Respiratory Respiratory Syncytial Virus or fragment thereof in complex with an RNA molecule, wherein the N proteins are organized in a distinct ring. Methods for producing such a N-RNA complex or crystal, to uses and applications thereof, including methods for identifying or developing a compound, which inhibits or prevents the replication and/or transcription of said virus and a computer system for such methods are claimed.
GB0916802A 2009-09-24 2009-09-24 A Paramyxoviridae family N protein-RNA complex in crystal form Withdrawn GB2475223A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB0916802A GB2475223A (en) 2009-09-24 2009-09-24 A Paramyxoviridae family N protein-RNA complex in crystal form
PCT/IB2010/002560 WO2011036563A2 (en) 2009-09-24 2010-09-16 Structure of the n-rna complex of the non-segmented negative-strand rna viruses of the paramyxoviridae family

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB0916802A GB2475223A (en) 2009-09-24 2009-09-24 A Paramyxoviridae family N protein-RNA complex in crystal form

Publications (2)

Publication Number Publication Date
GB0916802D0 GB0916802D0 (en) 2009-11-04
GB2475223A true GB2475223A (en) 2011-05-18

Family

ID=41327546

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0916802A Withdrawn GB2475223A (en) 2009-09-24 2009-09-24 A Paramyxoviridae family N protein-RNA complex in crystal form

Country Status (2)

Country Link
GB (1) GB2475223A (en)
WO (1) WO2011036563A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001471A1 (en) * 1990-07-24 1992-02-06 The Uab Research Foundation Methods of use of bovine respiratory syncytial virus recombinant dna, proteins vaccines, antibodies, and transformed cells
WO2002010459A2 (en) * 2000-07-27 2002-02-07 Biocryst Pharmaceuticals, Inc. Three dimensional structure of paramyxovirus hemagglutinin-neuraminidases and use thereof
WO2006117456A1 (en) * 2005-04-29 2006-11-09 Institut National De La Recherche Agronomique (Inra) Preparation of soluble n-protein/truncated p-protein complexes or n-proteins soluble in a virus of the paramyxoviridae family and use thereof in vaccines

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2899902B1 (en) 2006-04-18 2012-01-27 Agronomique Inst Nat Rech N-PROTEIN FUSION PROTEINS OF A PARAMYXOVIRIDAE-PROTEIN FAMILY VIRUS OF INTEREST AND USES THEREOF FOR VACCINATION AND INTRA-CELL VECTORIZATION

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992001471A1 (en) * 1990-07-24 1992-02-06 The Uab Research Foundation Methods of use of bovine respiratory syncytial virus recombinant dna, proteins vaccines, antibodies, and transformed cells
WO2002010459A2 (en) * 2000-07-27 2002-02-07 Biocryst Pharmaceuticals, Inc. Three dimensional structure of paramyxovirus hemagglutinin-neuraminidases and use thereof
WO2006117456A1 (en) * 2005-04-29 2006-11-09 Institut National De La Recherche Agronomique (Inra) Preparation of soluble n-protein/truncated p-protein complexes or n-proteins soluble in a virus of the paramyxoviridae family and use thereof in vaccines

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Int J Antimicrobial Agents, Vol 24, S242, PF Varela et al, "Crystals of a ribonucleoprotein complex of the respiratory syncytial virus nucleoprotein N", S242 *
Science, Vol 326, 2009, RG Tawar et al, "Crystal structure of a nucleocapsid-like nucleoprotein-RNA complex of Respiratory Syncytial Virus", 1279-1283 *

Also Published As

Publication number Publication date
WO2011036563A9 (en) 2011-06-23
GB0916802D0 (en) 2009-11-04
WO2011036563A2 (en) 2011-03-31
WO2011036563A3 (en) 2011-05-19

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732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20111117 AND 20111123

WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)