GB2469424A - Non-emulsion methods and masked biomolecules - Google Patents

Non-emulsion methods and masked biomolecules Download PDF

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Publication number
GB2469424A
GB2469424A GB1013752A GB201013752A GB2469424A GB 2469424 A GB2469424 A GB 2469424A GB 1013752 A GB1013752 A GB 1013752A GB 201013752 A GB201013752 A GB 201013752A GB 2469424 A GB2469424 A GB 2469424A
Authority
GB
United Kingdom
Prior art keywords
biomolecules
masked
emulsion methods
methods
amplifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB1013752A
Other versions
GB2469424B (en
GB201013752D0 (en
Inventor
Steven J Gordon
Daniel Stetson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intelligent Bio Systems Inc
Original Assignee
Intelligent Bio Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US12/370,205 external-priority patent/US8455401B2/en
Priority claimed from US12/370,154 external-priority patent/US8741815B2/en
Priority claimed from US12/370,125 external-priority patent/US20090298131A1/en
Application filed by Intelligent Bio Systems Inc filed Critical Intelligent Bio Systems Inc
Priority claimed from PCT/US2009/001034 external-priority patent/WO2009105213A1/en
Publication of GB201013752D0 publication Critical patent/GB201013752D0/en
Publication of GB2469424A publication Critical patent/GB2469424A/en
Application granted granted Critical
Publication of GB2469424B publication Critical patent/GB2469424B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.
GB1013752.9A 2008-02-19 2009-02-19 Non-emulsion methods and masked biomolecules Expired - Fee Related GB2469424B (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US2976908P 2008-02-19 2008-02-19
US2978008P 2008-02-19 2008-02-19
US2977808P 2008-02-19 2008-02-19
US12/370,205 US8455401B2 (en) 2008-02-19 2009-02-12 Methods and devices for making arrays
US12/370,154 US8741815B2 (en) 2008-02-19 2009-02-12 Methods and devices for amplification of nucleic acid
US12/370,125 US20090298131A1 (en) 2008-02-19 2009-02-12 Non-Emulsion Methods And Masked Biomolecules
PCT/US2009/001034 WO2009105213A1 (en) 2008-02-19 2009-02-19 Non-emulsion methods and masked biomolecules

Publications (3)

Publication Number Publication Date
GB201013752D0 GB201013752D0 (en) 2010-09-29
GB2469424A true GB2469424A (en) 2010-10-13
GB2469424B GB2469424B (en) 2013-04-17

Family

ID=42735329

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1013752.9A Expired - Fee Related GB2469424B (en) 2008-02-19 2009-02-19 Non-emulsion methods and masked biomolecules

Country Status (1)

Country Link
GB (1) GB2469424B (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020039728A1 (en) * 2000-02-10 2002-04-04 Robert Kain Alternative substrates and formats for bead-based array of arrays
US6585939B1 (en) * 1999-02-26 2003-07-01 Orchid Biosciences, Inc. Microstructures for use in biological assays and reactions
US20040258832A1 (en) * 2003-06-17 2004-12-23 Barklund Anna M. Method of chemical analysis using microwells patterned from self-assembled monolayers and substrates

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6440706B1 (en) * 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
NZ551996A (en) * 2004-05-13 2010-07-30 Anita Goel Nano-PCR: Methods and devices for nucleic acid amplification and detection

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6585939B1 (en) * 1999-02-26 2003-07-01 Orchid Biosciences, Inc. Microstructures for use in biological assays and reactions
US20020039728A1 (en) * 2000-02-10 2002-04-04 Robert Kain Alternative substrates and formats for bead-based array of arrays
US20040258832A1 (en) * 2003-06-17 2004-12-23 Barklund Anna M. Method of chemical analysis using microwells patterned from self-assembled monolayers and substrates

Also Published As

Publication number Publication date
GB2469424B (en) 2013-04-17
GB201013752D0 (en) 2010-09-29

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)

Free format text: REGISTERED BETWEEN 20180531 AND 20180606

PCNP Patent ceased through non-payment of renewal fee

Effective date: 20190219