GB2467436A

GB2467436A - Phosphoantigen for use in treatment of Burkholderia infection

Info

Publication number: GB2467436A
Application number: GB1001517A
Authority: GB
Inventors: Caroline Ann Rowland; Margaret Gillian Hartley; James Edward Eyles
Original assignee: UK Secretary of State for Defence
Current assignee: UK Secretary of State for Defence
Priority date: 2009-01-29
Filing date: 2010-01-29
Publication date: 2010-08-04
Also published as: GB201001517D0; GB0901423D0; WO2010086614A1

Abstract

The present invention relates to a phosphoantigen for use as a medicament, particularly in die.prophylaxis and/or treatment of infection by Burkholderia, including but not limited to Burkholderia pseudomallei and Burkholderia mallei and a pharmaceutical composition comprising such phosphoantigens. The phosphoantigen may be used for the treatment of melioidosis, and may be selected froth IPP, CHDMAPP, BrHPP or IHPP.

Description

Treatment The present disclosure relates to phosphoantigens for use in the prophylaxis and/or treatment of infection by I3urkholderia pseudoinaiiei, I3urkholderici,naiiei and/or Burkhok/eria spp for example Burkholderia pscudoinallei infection i.e. melioidosis, and pharmaceutical compositions comprising said phosphoantigen in particular for use in the prophylaxis andlor treatment of said infections.

Burkhoideria pseudornailei was first isolated by the British pathologist A. Whitmore from a 10 year old boy in Rangoon, Burma, who died of pncumonitis. Autopsy of further patients revealed tubercular lesions in the lung and abscesses on internal organs. Gram-negative rods were isolated in pure culture from the lesions, and identified by Whitmore as the causative agent of the disease. The glanders-like disease was subsequently named melioidosis and the pathogen I3acieriiun whitinori. After several changes of name, the pathogen was finally assigned to the Burkhoideria genus, a decision based on several criteria including I 6S RNA sequences and DNA homology. The Burkholderia genus consists of non-spore forming bacilli which are highly polymorphic. Only three species are pathogenic for man; B. pseudoinaiiei and Burkhoideria,nailei are capable o causing disease in otherwise healthy individuals whereas Burkholderia cepacia causes infections in inimunocompromised people and niay be responsible for cepacia syndrome in patients with cystic fibrosis. B. pseitdornaliei is closely related to B. ,nc,1/ei, the aetiological agent of glanders. B. rncillei is non-motile, while B. pseucloina/ici possesses polar flagellae and is motile.

B. pseudomaiiei has been isolated from water and soil in tropical countries, mainly between latitudes 20° North and South where the temperature does not fall below 4°C. Most of the * cases of melioidosis are identified in people from these regions, and patients developing melioidosis in temperate, non-endemic areas have usually lived or travelled in endemic regions. Endemic countries include Thailand, Vietnam, Cambodia. Southern China and Northern Australia, and of these Thailand has the highest number of reported cases.

Melioidosis is usually acquired by abrasion of the skin and infection by contaminated soil and water, and is thus frequently reported in rice farmers. Human-to-human and maternal transmission are rare. Inhalation of infectious dusts is another documented route of acquiring ** melioidosis. This was especially notable during the Vietnam War, when helicopter crewmen became infected. By 1973, following the withdraw of US forces from Vietnam, there had **.*..

* 35 been 343 reported cases of melioidosis resulting in 36 deaths. Melioidosis in many cases formed a latent infection which only recrudesced a significant time after return to the US, giving rise to the name "time-bomb disease" among ex-servicemen.

Melioidosis is the name given to any infection caused by B. pseuclornaiiei. The pathogen causes a wide spectrum of disease, ranging from the acute to chronic, and even latent infections which can last for decades. Melioidosis often presents with other conditions such as diabetes which can cause immunosuppression in the individual. Almost all organs and systems in the body can be affected, including the lungs. In Thailand, melioidosis is a significant cause of morbidity and death. Death is due to septic shock, respiratory failure and organ failure, occurring within days of onset of acute melioidosis. Intra-venous ceftazidime and imipenem are the antibiotic therapies of choice, and if given early in infection can reduce mortality rates to approximately 40 %. B. pseudomaliet is resistant to many of the other antibiotics currently available and relapse is common. The effectiveness of measures to reduce exposure to the causative organism have not been established and a vaccine is not available.

Given the debilitating nature of infection with Burkholderici, for example with B. pseudomaiiei, that no prophylactic treatmentivaccine is available which is suitable for use in the general public and the infections are difficult to treat, an effective prophylactic therapy andlor treatment of the infections would be useful.

The present disclosure provides a phosphoaiitigen for the prophylaxis andlor treatment of infection by Burkholderia pseudoinallei, Burkhoideria maiiei and Burkhoideria spp, for example, Burkhoideria,seudoinaiIei infection i.e. nielioidosis.

Brief Description of the Figures

Figure 1 shows human PBMCs treated with certain phosphoantigens reduce intracellular Burkholderia pseudomallei in vitro PBMCs cultured with IPP+IL-2 and CHDMAPP+IL-2 kill intracellular B. pseudoinaliei 708a. The human monocytic cell line THP-I was infected with B. pseudonialiei and PBMCs cultured with either media alone, IL-2 alone, IPP+IL-2 or CHDMAPP�IL-2 were added to the infected cells.. Some phosphoantigen-cultured cells received additional phosphoantigcn treatment with [PP (IPP+IL-2 -i-[PP) or CHDMAPP (CHDMAPP+IL-2 +Cl-IDMAPP) 4 Ii prior to the infection assay. Data is represented as the fold decrease in intracellular 13. pseudoinal/ei numbers as a ratio of intracellular bacterial numbers retrieved from control wells containing THP-I cells alone divided by intracellular bacterial numbers retrieved from wells containing cultured PBMCs for each individual. Data is retrieved from8 separate experiments with blood from 8 different individuals.

Figure 2 shows purified human gammadelta T cell activity against intracellular S..... . . . * . 35 Burkholderia pseudonciliei. is enhanced by treatment with phosphoantigen CHDMAPPPurified hLiman yS T cells kill intracellular B. pseudoinaiiei 708a in vitro which is enhanced by treatment with CHDMAPP. The human monocytic cell line THP-I was infected with B. pseudoinc:iIei and media alone (THP-I only) or purified human y T cells (obtained from one individual) were added at lx 106 or lx I 0 cells/mI and were either LinSlimulated or treated with CHDMAPP 4 11 prior to infection assay (+CHDMAPP). The graph shows the number of intracellular B. pseudoinailei cfu/ml retrieved under each condition as a mean of triplicate wells. Error bars indicate standard deviation.

Figure 3: Marmoset splenic cell preparations cultured with CHDMAPP+IL-2 kill intracellular B. pseudomciflei 708a. The human monocytic cell line (THP-I) was infected with B. pseudomailei. Media alone (THP-l only) or splenic cell preparations, cultured with lL-2 or CHDMAPP+1L-2 for tOdays, were added to infected THP-l at lx 106 cells/mI. The graph shows the number o intracellular /3. pceiedoina!iei (cm/mi) retrieved under each condition as a mean of triplicate wells from 3 different individuals. Error bars indicate standard deviation.

Whilst phosphoantigens have been suggested previously as potential adjuvants, there has been no suggestion that such compounds can be used in the treatment of a patient in their own right, that is, it has not been previously suggested that a treatment against any of the pathogens described herein could involve a phosphoantigen in the absence of an antigen, or if an antigen is present, that it is not pharmaceutically active in the prophylaxis and/or treatment of Burkhoideria infection, or it is not present in sufficient amounts to be pharmaceutically active in the prophylaxis and/or treatment of Burkholderia infection. As used herein, "adjuvant" refers to a non-antigenic substance or substantially non-antigenic substance that is used in combination with an antigen for enhancing the immune response against the antigen.

As used herein, "pharmaceutically active" nieans that a substance has a statistically significant measurable effect on the material/host to which is it is administered. The present invention is not intended to cover use of a phosphoantigen as an adjuvant in a medicarnent, such as in a medicament against any of the diseases mentioned herein. As used herein, "treatment" can mean prophylactic treatment (i.e. pre-treating) or treatment post-infection with a pathogen oithe type disclosed herein. A treatment is considered successful if the symptoms of an infection are ameliorated or prevented. *...

The present invention therefore relates to priming an individual with phosphoantigen to : provide beneficial therapeutic effects in relation to prevention of infection or amelioration of * * symptoms, after infection with a Burkhoideria infection. ** * * * * * **

The Vy9V2 T cells stimulated by the phosphoantigen are thought to have an important role *a* to play in the innate and adaptive immune response and may provide protective immunity (preventing infection of an individual) oi. ameliorate the symptoms of those infected as the * S body is stimulated to fight the infection.

*.S..* 0 * Most bacteria produce phosphoantigens as intermediates of the DOXP metabolic pathway.

Phosphoantigens are small molecular weight molecules with phosphorylated structures that selectively activate human or non-human primate T cells expressing Vy9VS2 T cell receptors.

Having said this it has not be shown definitively that Burkhoideria pseudonaiiei, Burkholderia,nallei andlor Biirkhoideria spp produces a phosphoantigen.

Surprisingly, the use of phosphoantigens seem particularly effective in the prophylaxis and/or treatment of infection by Burkholderia pseudonwtliei, Burkhoideria mailei and/or Burkhoideria spp. in particular i3urkholderia pseudorna/iei, i.e. rnelioidosis. This may be related to the fact that the bacteria are intracellular pathogens.

Phosphoantigens have been isolated from bacterial species such as TUBagI -4 from M. Tuberculosis.

Certain other phosphoantigens are known such as BrHPP and IHPP examples I and 2 respectively in US application publication No. 2004/0087555. The application relates to compounds of the following formula: 01-1 0 0 0

II II

X-CH2-C(CH2)O-PO-FOP-0Ca Ri 0Cat OCat OCat wherein: X is a halogen selected from I, Br or Cl, R' is selected from methyl and ethyl, and Cat is a cation, and ii is an integer between 2 and 20.

A particularly suitable structure therein is: 01-1 0 0

I II II

X-C1-12-C-(Cfl2)1--0-i,----0-i'-O---R3 RI OCat' OCiL' *** * . * 20 wherein X, Ri and Cat+ is as defined above and R3 is selected from: e*..�. * .

XCH2C(CH2)11 Cl-12O I Rl-C-(Cl-I2)-Ri * ;and **.. * S

C1-12 C-(Cl-I2)111--*S**.S * RI Below is a table of a number of phosphoantigens from said application.

MOLECULE

Name Ahbt'eviaLon Structure isopentenyl ll'i' Ct-I, PVFOI) hosphat e II Cl-h-C-----(Cl-12),---Oi'I' 3-(chloronicthyl)-Ci i-il'P Cl-l.,CI 3-btiianoi-i -vi I diphosphate CI-!3---C----(C112)2-OPP 0l-l 3-(bio monietlivl)-13 i-ill Cl-f,13r 3-butanol-] -vi I diphosphate Cl-h-C--(CI-i2)2-Oi'l Of-i 3-(iodomethyi)-3-I HPP CH2I butanoi-1-yl diphosphate CH3-C--(Cl-12)2--OPI' Of! 3-romomethyI)-13r1-IPPP CI-1213r 3-buLanoi-i-yl I triphosphate CHiC (CU2)2-OEPI' OF! 3-(iodoniethyl)-3-I Fil'l'l' CHi butaiioi-i-vi I triphosphate CH3C-(CFI2)2-OPPP 01-I a, y di-3-di-131 1-ITI' CH2I3r CH213i' (bromometh vl)-3-I hutapiol-i-yi i-lC-C-(Cl-i2)2-Ol'i'i'O--Cff2)2-CCl-i3 triphosphatc I 01-i 01-I a. -y di-3-di-iI-rri' Cl-I,! Cli,! (iodomethvl)-3-I *** * butanoI-1-vI i-i3C-C-(Ci-l,)2-OPi'I'O----CFi2)-C-cl-i3 * * triphosphate I **S* 01-I Oil * * S * wherein is P phosphate.

S Other compounds explicitly disclosed in the case include 3-(bromomethyl)-3-butanol-I-yl : 5 triphosphate (BrHPPP); 3-(iodomethyl)-3-butanol-I-yl triphosphate (IHPPP); uridine 5'- triphosphate gamnia-[3-methyl-3butene-l -yli; alpha, beta di[3-brornomethyl-3-butanol-I-*.* yl]diphosphate. S..

* An alternative phosphoantigen is C-IPP HOCH2-O-ONH4 ONH4 ONH4 (3-methylbut-3-eiiyl pyrophosphonate); 0 0 HOOP-O-P-ONH4

II II

YNH4 YNH4 HIIPP; O 0

O-P--O-P--OH

II II

I I

OH OH

HT1gIyIPP °r°°

H

II II

OH OH

HAngeIylPP A particularly potent phosphoantigen is CHDMAPP: L°° * * O-04-OH *

I I

* . OH OH OH io * * * * S. * Certain phosphoantigens are described in published patent application publication number

S

US2008/0207568 (Innate Pharma) which describes certain phosphoanhigens of the formula: I... * S S...

*5SS*S * . (1) R5-W = C -R7 R4 OCa( OCat cli) R6 R3 r° 1 " "S I ill I II CC--C-A-+P--13-4---P-Y; / I I II I I R7 R4 L OCat j 0Cat1 Ill Ull) ro 1

I III I II

R5-NCCATPBTPY; 01 (V) -w C A J_ B I I [I j CH3 R4 OCai OCat wherein Cat+ represents one oi-more cations In is an integer I to 3, B is 0, NH, CHF, CF2 or CH2 01-any other isosteric group, W is C-R6 or N, R7 is a C1 alkyl group or any other isosteric group such as CF1, R3, R4 and R6 al-c independently selected from H, C13 alky or any other isosteric group such S...

*.,** asCFi, R5 is selected from C23acy1, aldehyde, a C1.3 alcohol or a C23 ester, and * Y=OCat+ is as defined therein. 5

Any suitable phosphoantigen may be employed for the prophylaxis or treatment of said infection, including a synthetic andlor natural phosphoantigen. Particularly suitable phosphoantigens are described above, such as CHDMAPP and IPP. * *

: Prophylaxis as employed hei-ein refers to wherein the individual has a teduced risk of * infection, i.e. invasion and multiplication by Burkhoideria p.veudo,nal/ei, I3urkho/derici mallei andlor Burkho/derici,vpp. in particular l3urkhoiderici pseudoina//el. lii one eni bodi ment the prophylactic treatment results in a reduced risk of developing melioidosis.

Reduced i-isk as employed herein refers to a 20, 30, 40, 50, 60, 70, 80% or more i-educed risk of the developing the infection or a severe form of the infection compared to an individual who has not had any prophylactic treatment against the relevant pathogen.

Severe infection as employed herein is infection that under the present prescribing habits/protocols would warrant treatment with a combination of chloramphciiicol, doxycycline and co-trimoxazole and would include persistent infection of the blood.

In one embodiment there is a provided a phosphoanhigen for use in the treatment or prophylaxis of melioidosis.

Thus in one aspect, the disclosure provides a method of treating infections described herein, particularly nielioidosis, comprising administering a therapeutically effective amount of the phosphoantigen prophylactically 01. to a patient infected by Burkhoideria pseudomallei.

Burkhoideria nwliei andlor Burkhoideria spp, in particular Burkhoiderict pseudomalici.

In one embodiment the phosphoantigen is administered before infection, for example 1, 2 or 3 days or a week or a month before exposure.

In one embodiment the phosphoantigen in given shortly after exposure, inoculation or infection, for example I hour to 3 days, such as 2 to 24 hours after.

In one embodiment the phosphoantigen is administered before exposure and shortly after exposure, particularly as per the time frames above.

In one embodiment the phosphoantigen is employed for the treatment of chronic infection, for example rnelioidosis, for example as a monthly treatment regime.

In one aspect there is provided use of a phosphoantigen for the manufacture of a inedicament for the treatment of Burkhoideria pseudoinaiiei, Bitrkholderia maiiei and/or Burkhoideria spp infection, in particular Burkholderia pseudornaiiei infection i.e. melioidosis.

In one embodiment a combination of a phosphoantigen and human interleukin-2 (IL-2) are employed. S. * * S S * S.

The in vitro data with the relevant pathogens indicates that the bacterial load in a human monocytic cell line is reduced 10 to 1000 fold in the presence of human blood or purified yö *.... 35 T cells treated with phosphoantigen and IL-2 cx vivo. Given that the cells employed in the in vitro assay are retrieved from the blood of individuals' in an approximation of the in vim'o mechanism gives confidence that the phosphoantigens will work by the same mechanism in vivo.

In one embodiment the disclosure relates to a pharmaceutical coniposihloll coiiipnsillg phosphoantigen in the presence or absence of IL-2, along with a pharmaceutically accepiable excipient, for example for the treatment or prophylaxis, in particular as described herein. The composition may be employed as described above for the phosphoantigen.

In one embodiment the phosphoantigen is employed in combination with IL-2 or an alternative or further cytokine such as IL-15, IL-2 I interferon-y and/or interferon-a.

In alternative embodiment the disclosure also relates to a composition comprising a phosphoantigen and an adjuvant and optionally in combination with IL-2 or an alternative or further cytokine such as IL-iS, IL-21 interfcron-y and/or interferon-a.

The active components of the combinations of the disclosure may, for example be co-formulated if they stable when mixed together. Alternatively, the components may be formulated separately and co-administered, that is administered at the same time or approximately the same time, for example one immediately after the other. In a further alternative the components may be administered sequentially, that is to say with a delay between the administrations, for example a delay of I to 12 hours.

Formulations The phosphoantigens or compositions according to the present disclosure maybe administered orally, topically, parenterally, transdermally, as a suppository or by any other pharniaceuticall y appropriate route.

Typical delivery routes include parenteral administration, e.g., intradernial, intramuscLllar or subcutaneous delivery. Other routes include oral administration, iniranasal, intravaginal routes, intraderm al and transdcrmal administration.

In one embodiment the phosphoantigen according to the disclosure is provided optionally in either as a lyophilized formulation for reconstitution later or as a liquid formulation.

Trausdermal administration, such as by iontophoresis, may also be an effective method to deliver phosphoantigen to muscle. Epidermal administration may also be employed. Thus the ***... . * . 30 disclosure also extends to delivery by a transdernial patch, which may be occlusive or non-occiussive. *. S * * S * **

:. The actives can also be formulated for administration via the nasal passages. Formulations suitable for nasal administration, wherein the carrier is a solid, include a coarse powder **.** 35 having a particle size, for example, in the range of about 10 to about 500 microns which is administered in the manner in which snuff is taken, i.e., by rapid inhalation through the nasal passage from a container of the powder held close up to the nose. Suitable formulations wherein the carrier is a liquid for administration as, for example, nasal spray, nasal drops, or by aerosol administration by nebulizer, include aqueous or oily solutions of the active ingredient. For further discussions of nasal administration of AIDS-related vaccines, references are niade to the following patents, U.S. Pat. Nos. 5,846,978, 5,663,169, 5,578,597, 5,502,060, 5,476,874, 5,413,999, 5,308,854, 5,192,668, and 5,187,074.

Compositions of use in the disclosure include liquid preparations, for an orifice, e.g., oral, nasal, anal, vaginal, etc. administration, such as suspensions, syrups ot. clix irs; and, preparations for parenteral, subcutaneous, intradermal, intramuscular or intravenous administration (e.g.. injectable administration) such as sterile suspensions or emLilsions.

In compositions of the disclosure the relevant active ingredient may he in admixture with a suitable carrier, diluent, or excipient such as sterile water, physiological saline, glucose or the like.

The active ingredients can be incorporated, if desired, into liposomcs, microspheres or other polymer matrices (Feigner et al., U.S. Pat. No. 5,703,055; Gregoriadis, Liposome Technology, Vols. Ito III (2nd ed. 1993), each of which is incorporated herein by reference).

Liposomes, for example, which consist of phospholipids or other lipids, are nontoxic, physiologically acceptable and metabolizable carriers that are relatively simple to make and administer.

Liposome carriers may serve to target a particular tissue or infected cells, as well as increase the half-life of the active. Liposomes include emulsions, foams, micellcs, insoluble monolayers, liquid crystals, phospholipid dispersions, lamellar layers and the like. In these preparations the vaccine to be delivered is incorporated as part of a liposome, alone or in conjunction with a molecule which binds to, e.g., a receptor prevalent among lymphoid cells, such as monoclonal antibodies or with other therapeittic or immunogenic compositions. Thus, liposomes either filled or decorated with a desired immunogcn of the disclosure can be directed to the site of lymphoid cells, where the liposomes then deliver the immunogen(s).

Liposomes may be formed from standard vesicle-forming lipids, which generally include neutral and negatively charged phospholipids and a sterol, such as cholesterol. The selection of lipids is generally guided by consideration of, e.g., liposome size, acid lability and stability of the liposomes in the blood stream. A variety of methods are available for preparing liposomes, as described in, e.g., Szoka, eta!., Ann. Rev, Biophys. Bioeng. 9:467 (1980), U.S. Pat. Nos. 4,235,871, 4,501,728, 4,837,028, and 5,019,369.

SI....

* * 30 The liposornes generally contain a neutral lipid, for example phosphatidylcholine, which is : usually non-crystalline at room temperature, for example egg yolk phosphatidylcholine, dioleoyl phosphatidyicholine or dilauryl phosphatidylcholine.

* 35 In one embodiment the pharmaceutical formulation comprising the phosphoantigen is I.., buffered, for example to a pH in the range 6.8 to 7.8.

I..... * .

In one embodiment the pharmaceutical formulation comprises a stabilizer, for example human serum albumin, normal serum albumin andlor human plasma protein fraction.

in some embodiments optionally the formulation may comprise an adjuvant, for example a known adjuvant formulation may be used to reconstitute the formulation.

Antigens employed iii the disclosure may be mixed or adsorbed with adjuvants, which include but are not limited to alum, murainyl dipeptide and saponins such as Quit A. This may further boost the immune system's ability to deal with the infectioti.

Particular adjuvants are those selected from the group of metal salts, oil in water emulsions, Toll like receptors agonist, (in particular Toll like receptor 2 agonist, Toll like receptor 3 agonist, Toll like receptor 4 agonist, Toll like receptor 7 agonist, Toll like receptor 8 agonist and Toll like receptor 9 agonist), saponins or combinations thereof. The level ofree antigen in a given formulation may be increased by, for example, formulating the composition in the io presence of phosphate ions, such as phosphate buffered saline, or by increasing the ratio of antigen to metal salt. In one embodiment the adjuvant does not include a metal salt as sole adjuvant. In one embodiment the adjuvant does not include a metal salt.

In an embodiment the adjuvant is a Toll like receptor (TLR) 4 ligand, for example an agonist such as a lipid A derivative, in particular monophosphoryl lipid A or more specifically 3-deacylated monophoshoryl lipid A (3D-MPL).

3-Deacylated monophosphoryl lipid A is known from US patent No. 4,912,094 and UK patent application No. 2,220.211 (Rihi) and is available from Rihi Immunochem, Montana, USA.

3D-MPL is sold under the trademark MPL� by Corixa corporation and primarily promotes CD4-i-T cell responses with an IFN-g (Thi) phenotype. It can be produced according to the methods disclosed in GB 2 220 211 A. Chemically it is a mixture of 3 -deacylated monophosphoryl lipid A with 3, 4, 5 or 6 acylated chains. Generally in the compositions of the present disclosure small particle 3D-MPL is used. Small particle 3D-MPL has a particle size such that it may he sterile-filtered through a 0.22tm filter. Such preparations are described in International Patent Application No. WO 94/2 1292.

S.. 55.

* 30 Synthetic derivatives of lipid A are known and thought to be TLR 4 agonists including, but not limited to: * S 5 * 0M174 phosphono-3-D-g1ucopyranosyli -2-[(R)-3-hydroxytetradecanoyl aminoi-u-D-glucopyranosyldihydrogenphosphate), (WO 95/14026).

* 35 OM 294 DP (3S, 9 R) 3[(R)dodecanoyloxytetradecanoylamiflOi-4-OX0S-aZa9(R) [(R)-3 - *....: hydroxytetradecanoylamiriOdecali-I, 1 0-diol, I, I 0-bis(dihydrogenophosphate) (WO * 99/64301 and WO 00/0462).

OM 197 MP-Ac DP (3S, 9R)-3-[(R)-dodecanoyloxytetradecanOYlarn inoi-4-oxo-5-aza-9- [(R)-3-hydroxytetradecanoylamiflOi decan-I, 1 0-diol, I -dihydrogenophosphate I 0-(6-aniinohexanoate) (WO 0 1/46127).

Typically when 3D-MPL is used the antigen and 3D-MPL are delivered with aluni or presented in an oil in water emulsion or multiple oil in water emulsions. The incorporation of 3D-MPL is advantageous since it is a stimulator of effector T-cell responses. Alternatively the 3D-MPL may be formulated as liposomes.

Other TLR4 ligands which may be used are alkyl glucosaminide phosphates (AGPs) such as those disclosed in WO 98/50399 or US 6303347 (processes for preparation of AGPs are also disclosed), or pharmaceutically acceptable salts of AGPs as disclosed in US 6764840. Some AGPs are TLR4 agonists, and some are TLR4 antagonists. Both are thought to be useful as adjuvants.

Another immunostimulant for use in the present disclosure is Quil A and its derivatives. Quil A is a saponin preparation isolated from the South American tree Quilaja Saponaria Molina and was first described as having adjuvant activity by Dalsgaard et al. in 1974 (Saponin adjuvants', Archiv. fur die gesamte Virusforschung, Vol. 44, Springer Verlag, Berlin, p243- 254). Purified fragments of Quil A have been isolated by HPLC which retain adjuvant activity without the toxicity associated with Quil A (EP 0 362 278), for example QS7 and QS2I (also known as QA7 and QA2I). QS2I is a natural saponin derived from the bark of Quillaja saponaria Molina which induces CD8+ cytotoxic T cells (CTLs), Th1 cells and a predominant IgG2a antibody response.

Particular formulations oQS2I have been described which further comprise a sterol (WO 96/33739). The ratio of QS2 I:sterol will typically be in the order of I: 100 to I: I weight to weight.

Generally an excess of sterol is present, the ratio of QS2 I:sterol being at least I. :2 w/w.

Typically for human administration QS2I and sterol will be present in a vaccine in the range of about 1 jig to about 100 jig, such as about 10 jig to about 50 jig per dose.

A formulation comprising QS2I and liposomes may be prepared, for example containing a charged lipid, which increases the stability of the lipsonie-QS2 I structure for liposomes composed of saturated lipids. In these cases the amount of charged lipid is often 1-20% w/w, such as 5-10%. The ratio of sterol to phospholipid is 1-50% (mol/mol), such as 20-25%. *

These compositions may contain MPL (3-deacylated mono-phosphoryl lipid A, also known as 3D-MPL).

The saponins may be separate in the form of micelles, mixed micelles (generally, but not exclusively with bile salts) or may be in the form of ISCOM matrices (EP 0 109 942), liposomes or related colloidal structures such as worm-like or ring-like multimeric complexes or lipidic/layered structures and lamellae when formulated with cholesterol and lipid, or in the form of an oil in water emulsion (for example as in WO 95/172 10). The saponiris may often be associated with a metallic salt, such as aluminium hydroxide or aluminium phosphate (WO 98/15287).

Usually, the saponin is presented in the form of a liposome, ISCOM or an oil in water emulsion.

Immunostimulatory oligonucleotides may also be used. Examples of oligonucleotides for use in adjuvants of the present disclosure include CpG containing oligonucleotides, generally containing two or more dinucleotide CpG motifs separated by at least three, more often at least six or more nucleotides. A CpG motif is a cytosine nucleotide followed by a guanine nucleotide. The CpG oligoiiucleotides arc typically deoxyiiucleotides. In one embodiment the internucleotide in the oligonucleotide is phosphorodithioate, or more preferably a phosphorothioate bond, although phosphodiester and other internucleotide bonds are within the scope of the disclosure. Also included within the scope of the disclosure are oligonucleotides with mixed internucleotide linkages. Methods for producing phosphorothioate oligonucleotides or phosphorodithioate are described in US 5,666,153, US 5,278,302 and WO 95/26204.

Exan�^ples of oligonucleotides are as follows: TCC ATG ACG TTC CTG ACG TT (CpG 1826) TCT CCC AGC GTG CGC CAT (CpG 1758)

ACC CAT GAC GTC GCC GGT GAC CCC ACC ACG

TCG TCG TTT TGT CGT TTT GTC GTT (CpG 2006) TCC ATG ACG TTC CTG ATG CT (CpG 1668) TCG ACG TTT TCG GCG CGC CCC C (CpG 5456), the sequences may contain phosphorothioate. modified internucleotide linkages.

Alternative CpG oligonucleotides may comprise one or more sequences above in that they have inconsequential deletions or additions thereto.

S.. .** * * The CpG oligonucleotides may be synthesized by any method known in the ait (for example * S. . 0 : 30 see EP 468520). Conveniently, such oligonucleotides maybe synthesized utilising an automated synthesizer.

Examples o a TLR 2 agonist include peptidoglycan or lipoprotein. Imidazoquinolines, such as Iniiquimod and Resiquimod are known TLR7 agonists. Single stranded RNA is also a * * 35 known TLR agonist (TLR8 in humans and TLR7 in mice), whereas double stranded RNA and poly IC (polyinosinic-polycylidylic acid -a commercial synthetic mimetic of viral RNA) are exemplary o TLR 3 agonists. 3D-MPL is an example of a TLR4 agonist whilst CpG is an example of a TIR9 agonist.

An imrnunostimulant may alternatively or in addition be included. In one embodiment this immunostimulant will be 3-deacylated monophosphoryl lipid A (3D-MPL).

Adjuvants combinations include 3D-MPL and QS2I (EP 0 671 948), oil in water emulsions comprising 3D-MPL and QS2I (WO 95/172 10, WO 98/56414), or 3D.-MPL formulated with other carriers (EP 0 689 454) including liposomes. Other adjuvant systems comprise a combination of 3D-MPL, QS2I and a CpG oligonucleotide as described in US 6558670 and US 65445 18.

in one aspect the adjuvant comprises 3D-MPL. In one aspect the adjuvant comprises QS2I.

in one aspect the aduvant comprises CpG. In one aspect the adjuvant comprises QS2 I and 3D-MPL. In one aspect the adjuvant comprises QS2 I, 3D-MPL and CpG. In one aspect the adjuvant is formulated as an oil in water emulsion.

In one aspect the adjuvant is formulated as liposomes.

The amount of 3D-MPL used is generally small, but depending on the vaccine formulation may be in the region of I to I0001g per dose, generally I to 500tg per dose, and more such as between I to 10Oig per dose (10, 20, 30, 40, 50, 60, 70, 80 or 90ig per dose).

The amount o CpG or immunostimulatory oligonucleotides in the adjuvants or vaccines of the present disclosure is generally small, but depending on the vaccine formulation maybe in the region of I to 1000ig per dose, generally 1 to 5001g per dose, and more such as between I to 100 j.ig per dose (10, 20, 30, 40, 50, 60, 70, 80 or 90g per dose).

The amount of saponin for use in the adjuvants of the present disclosure may be in the region of 1 to 1000 jig per dose, generally 1 to 500 jig per dose, more such as I to 250jg per dose, and more specifically between ito lOOp.g per dose (10, 20, 30, 40, 50, 60, 70, 8Oor 9Ojtg per dose). * .

Thus in one embodiment there is provided a formulation comprising phosphoantigen and 30 MPL, LPS ot. a derivative thereof.

In one embodiment there is provided a formulations comprising phosphoantigen and QS2 I In one embodiment there is provided a formulation comprising phosphoantigen and CpG.

* . 35 Thus in one embodiment there is provided a formulation comprising phosphoantigen and MPL and QS2I. Thus in one embodiment there is provided a formulation comprising phosphoantigen and MPL and CpG. Thus in one embodiment there is provided a formulation comprising phosphoantigen and QS2 1 and CpG. Thus in one embodiment there is provided a formulation comprising phosphoantigen and MPL, QS2 I and CpG.

Adjuvant as employed herein is nor intended to refer to the phosphoantigen element of the corn position.

In one embodiment the formulation/composition is a vaccine.

Vaccine preparation is generally described in New Trends and Developments in Vaccines, edited by Vollerercii., University Park Press, Baltimore, Maryland, U.S.A., 1978.

Encapsulation within liposonies is described, for example, by Fullerton, U.S. Patent 4,235,877.

in one embodiment the formulation is provided as a formulation for topical administrations including inhalation.

Suitable inhalable preparations include inhalable powders, metering aerosols containing propellant gases or inhalable solutions free from propellant gases. Inhalable powders according to the disclosure containing the active substance may consist solely of the abovementioned active substances or of a mixture of the abovementioned active substances with physiologically acceptable excipient.

These inhalable powders may include monosaccharides (e.g. glucose or arabinose), disaccharides (e.g. lactose, saccharose, maltose), oligo-and polysaccharides (e.g. dextranes), polyalcohols (e.g. sorhitol, mannitol, xylitol), salts (e.g. sodium chloride, calcium carbonate) or mixtures of these with one another. Mono-or disaccharides are preferably used, the use of lactose or glucose, particularly but not exclusively in the form of their hydrates.

Particles for deposition in the lung require a particle size less than 10 microns, such as 1-9 microns for example from 0.1 to 5.tm, in particular from I to 5.tni. The particle size of the active (that is the antigen is of primary importance). ** .

: 30 The propellcnt gases which can be used to prepare the inhalable aerosols arc known in the art.

Suitable propellent gases are selected from among hydrocarbons such as n-propane, n-butane or isobutane and halohydrocarbons such as chlorinated and/or fluorinated derivatives of methane, ethane, propane, butane, cyclopropane or cyclobutane. The abovementioned S...

* propellent gases may be used on their own or in mixtures thereof.

* S 35 Particularly suitable propellent gases are halogenated alkane derivatives selected from among TO II, TO 1 2, TO I 34a and TG227. Of the abovementioned halogenated hydrocarbons, TO I 34a (1,1,1,2-tetrafluoroethane) and TG227 (1,1,1,2,3,3,3-heptaliuoropropane) and mixtures thereof are preferred according to the invention.

The propelLent-gas-containing inhalable aerosols may also contain other ingredients such as cosotvents, stabilisers, surface-active agents (surfactants), antioxidants, lubricants and means for adjusting the pH. All these ingredients are known in the art.

The propellant-gas-containing inhalable aerosols according to the invention may contain up to 5 Wa by weight of active substance. Aerosols according to the invention contain, for example, 0.002 to 5 Wa by weight, 0.01 to 3 Wa by weight, 0.015 to 2 Wa by weight, 0.1 to 2 Wa by weight, 0.5 to 2 Wa by weight or 0.5 to I Wa by weight of active.

In one embodiment of the disclosure the phosphoantigen is employed in a prime boost regime, as the priming and/or boosting dose. Priming in this context refers to priming the immune system for a response, such as Vy9V82 T cell proliferation and/activation. Boosting refer to increasing or sustaining the immune response of said priming.

Whilst not wishing to be bound by theory if the appropriate number of doses is exceeded in a relatively short period of time then it is possible induce anergy in the immune system, which is undesirable and should be avoided.

In theoneembodimentthedose is intherange lpgto l000pgperKg, such as Ingin 10 pg per Kg.

In one embodiment the disclosure provides use of a phosphoantigen for enhancing protective host immune responses specifically those mediated by V7982 T cells to enable to the host to fight infection with Burlcholderia pseudomalle6 Burkholderia mattel and/or Burkhoideria spp infection, in particular Burkholderia pseudomaliti infection.

In one embodiment the disclosure provides a method of stimulating an immune response to fight Burkhotderiapseudomallet Burkholderia mattel and/or Burkhotderia spp infection, in particular Burkholderia pseudomallei infection.

c'. :30 In one embodiment the bioburden is Lowered in vivo after administration of a phosphoantigen

* of the disclosure. *.

S

In one aspect the disclosure provides a method of treating cepacia syndrome in cystic fibrosis patients by administering a therapeutically effective amount of a phosphoantigen or composition described herein.

The disclosure also relates to use of said phosphoantigens and composition for the treatment of cepacia syndrome in cystic fibrosis patients.

EXAMPLES

Materials and methods Preparation of Peripheral Blood Mononuclear Cells (PBMCs) Human blood (óOmt) was collected into BD Vacutainer CPT sodium citrate tubes and centrifuged (25mm; 1500g (no brake)). Buffy coats containing PBMCs were removed and centrifuged (300g; 15mm). The PBMC pellet was resuspended in lOmI medium (RPMI +10% Foetal calf serum (FCS), 200U/ml penicillin, 200tg/ml streptomycin and L-glutamine (10mM)). PBMCs were counted with trypan blue, centrifuged (300g; 15mm) and re-suspended in appropriate media to lx 106 cells/mI.

Culture of PBMCs with Phosphoantigen + IL-2 PBMCs were cultured for 10-14 days in media [RPMII64O, 200U/ml penicillin, 200ig/ml streptomycin and L-glutamine (10mM) and 10% Foetal Calf Serum (FCS) from selected sources] containing the synthetic phosphoantigens isopentenyl pyrophosphonate (1PP) (3tg/rnl) (Sigma, UK) or CHDMAPP (I l3nM) (Innate Pharma, France) in the presence of human recombitiant interleukin-2 (IL-2) (Sigma, UK) (lOOU/ml) to allow specific proliferation of Vy9V2 T cell populations. PBMCs were also cultured with either IL-2 (IOOU/ml) only or media alone. PBMCs were cultured under sterile conditions in 25cm3 cell culture flasks and media containing IL-2 (IOOU/ml) was added to flasks every 3-4 days (excepting PBMCs treated with media only) and were supplemented with fresh media without IL-2 where necessary.

Preparation of marmoset splenic cell suspensions Spleens, removed posthumously from marmosets, were mashed through 40im cell sieves and red blood cells were lysed using 0.85% ammonium chloride. Cells were centrifuged at 300g for 10 miii and cells were resuspended in 10 ml medium (RPM! +10% Foetal calf serum (FCS), 200 U/mI penicillin, 200 jig/mI streptomycin and L-glutamine (I 0mM)). Splenic cell suspensions were counted with trypan blue, centrifuged (300g; I 0mm) and resuspended in appropriate media to lx 106 cells/nil.

Culture of marmoset splenic cell suspensions with CHDM APP + IL-2 * * cells were cultured for 10 days in media [RPMI 1640, 200 U/ui I pen ici II in, 200 jig/mI *. streptornycin and L-glutamine (10 mM) and 10% Foetal Calf Serum (FCS) from selected sources] containing the synthetic phosphoarutigen CHDMAPP (113 nM) (Innate Pharma, France) and human recombinant interleukin-2 (IL-2) (Sigma, UK) (100 U/nil) to allow specific proliferation of Vy9V2 T cell populations. Cells were also cultured with IL-2 (100 U/mI) only. Cells were cultured under sterile conditions in 25cm3 cell culture flasks and media containing IL-2 (100 U/nil) was added to flasks every 3-4 days. Cells were supplemented with fresh media without IL-2 where necessary.

THP-i human,nonocy(e cell line The non-adherent human monocytic cell line THP-I (ATCC�) (ECACC, UK) was grown in RPM!, 10% Foetal calf serum (FCS) and L-glutamine (10 mM) all obtained from Sigma, UK in cell culture flasks under sterile conditions. Prior to infection, THP-l cells were removed from cell culture flasks and centrifuged (300g;l5min). Cells were resuspended in medium without antibiotics, counted and readjusted to required cell concentration (2x lO6cells/ml).

THP-1 cells (1 mI/well) were plated into 24 well plates and activated with PMA (phorbol 12-myristate 13-acetate; Sigma, UK) at a final concentration of long/mi and were incubated overnight (37°C; 5%C02) to produce an adherent monolayer. Media was removed from wells prior to infection and replaced with fresh media (900p1) without antibiotics.

Growth of B. pseudomallei strain 708a All work with B. pseudoino/Iei was performed at ACDP containment level 3. A viable frozen culture of B. pseudonw1/ei 708a, a clinically isolated gentamycin-sensitive strain, was thawed and was streaked across dried L-agar and incubated overnight at 37°C; CO2. The bacterial culture was then harvested with a sterile loop into sterile PBS to give a uiiiloini, cloudy suspension with an OD45011 reading between 0.3 100.4 of approximately 5xlO8ciu/ml. A one in 10 dilution of B. pseudoma/Iei 708a suspension was performed prior to infection of THP-1 cells.

Intracellular infection of TIJP-1 monolayer wit/i B. pseudomallei 708a B. pseudo#naiiei 708a suspension (100 p1/nil) was added to the THP-l monolayer and incubated (30 mm; 37°C; C02). Media was then removed and fresh niedia with 30 tg/nil gentarnycin was added to kill any extracellular bacteria and were reincubated for a further 30 nun. Cultured PBMCs (lx l0 cells/mI) or marmoset splenic cell suspensions (lx 106 cells/mI) were resuspended to an appropriate cell concentration in media containing l0tg/ml gemamycin or media only containing 10 tg/ml gentamycin were added to the infected THP-i monolayer and incubated in a scaled container (with CO2 packet) in 37°C incubator for 24 h. Supernatants were removed from wells following incubation and stored at -20°C. In order to retrieve intracellular bacteria, TI-IF-I were lysed using sterile distilled water (1 ml), pipetting up and down several times to disrupt cells. The lysed cell suspension (100 p1) was added to PBS (900 p1) to prevent further cell lysis. Droplets of the lysed cell suspension (3x20 pi) 3D stabilised in PBS were placed onto duplicate dried L-agar plates and incubated for 2-3 days (37°C; 5% CO2). Viable colonies were then counted to determine the number of viable intracelullar bacteria. S... * * *.. * * .

Statistical analysis Data retrieved from infection assays with human PBMCs was analysed using a paired Student's t-test followed by a Bonferroni's multiple comparison correction, Data retrieved from infection assays with marmoset cell suspensions was analysed using a one-way ANOVA followed by Bonferroni's multiple comparison tests.

Results Human PBMCs cultured with phospiwantigen kill intracellular B. pseudomallei The effect of human blood, cultured with rPP+IL-2 or CHDMAPP+IL-2, on intracellular growth of B. pseudonallei 708a was investigated. The data is presented as a fold decrease in bacterial numbers for each individual experiment (n=8). (Figure 1). A significant reduction in intracellular bacterial numbers was observed in the presence of PBMCs cultured with both CHDMAPP+IL-2 or IPP+IL-2 in comparison with PBMCs cultured with media alone (p<O.O 1). In addition to this, a significant reduction in intracellular B. pseudomctliei was observed following treatment with CHDMAPP�IL-2 (p<O.Ol) or JPP+JL-2 (p<O.OS) in comparison with PBMCs treated with IL-2 alone.

The effect of additional treatment with either IPP or CHDMAPP following culture,4 hours prior to use in the infection assay, was also investigated. Addition of CHDMAPP (I 13 nM) toCHDMAPP+IL-2 cultured PBMCs enhanced the reduction in intracellular bacterial numbers when compared with CHDMAPP+IL-2 alone.

Purified human yÔ T cells kill intracellular B. pseucloniaiiei In order to determine if yö T cells were important in the reduction of intracellular bacterial numbers following treatment with phosphoantigen, the ability of purified peripheral human *::::* y T cells to reduce 13. pseudoinallei numbers was investigated. Purified y T cells (I x lO and lx l0 cells/mi) reduced intracellular bacterial numbers significantly (p<O.Ol). Treatment of * purified y I cells with CFIDMAPP 4 hours prioi' to use in the killing assay significantly enhanced the reduction in bacterial numbers at 1x106 cells/mI leading to a 1000-fold reduction in bacterial numbers (Figure 2).

S

Marmoset splenic cells cultured with phosphoantigen kill intracellular B. pseudoinallei Marmoset splenic cells from 3 individuals were cultured with CHDMAPP+IL-2 or IL-2 alone and the effect of these cultured cclls on growth of intracellular /3. pseudoinaiiei 708a in THP-l cells was investigated. A 2-3 log reduction in intracellular bacterial numbers was observed in the presence of cells treated with CHDMAPP+IL-2 in comparison with cells cultured with IL-2 alone (p<O.OOl).

Claims

Claims 1. A phosphoantigen for the prophylaxis and/or treatment of Burkhoideria pseudornallei, Burklwiderici,ncillei and/or Bu rkholderiaspp infection.
2. A phosphoantigen according to claim I, for the prophylaxis and/or treatment of Bu rkho/deric, pseitdoinci/Iei in fed ion.
3. A phosphoantigen for according to claim 2 for the prophylaxis or treatment of melioidosis.
4. A phosphoantigen according to any one of claims I to 3, wherein the phosphoantigen is IPPor CHDMAPP.
5. A phosphoantigen according to claim I, where in the phosphoantigen is Brl-IPP or IHPP.
6. A pharmaceutical composition comprising a phosphoantigen and a pharmaceutical acceptable excipient.
7. A pharmaceutical composition according to claim 6, wherein the excipient is an adj uvant.
8. A pharmaceutical composition according to claim 6 or 7, for the prophylaxis and/or treatment of i3urkholderia pseudoincil/et, Burkholderia inn/let and/or I3urkholclerici S/J infection
9. A pharmaceutical composition according to claim 8, for the prophylaxis andlor treatment of Burkho/derici pseudoinaliei infection. S... * .
10. A pharmaceutical composition according to claim 9, for the prophylaxis and/or *.... treatment of melioidosis.
ii. A method of treatment comprising administering a therapeutically effective amount of :. a phosphoantigen or a pharmaceutical composition comprising same prophylactically or as treatment to a patient in need thereot for Burkhoideria pseudonwti/ei, Burkhoideria mci//el and/or i3urkho/deria spj infection. *...*:
12. A method according to claim 11, for the prophylaxis or treatment for!3urkhoideria pseudoma/le: infection.
13. A method according to claim II, wherein the pharmaceutical composition does not further comprise an antigen that is pharmaceutically active in the prophylaxis and/or treatment of Burkhok/erici infection.
14. A pharmaceutical composition comprising a phosphoantigen for the prophylax is andlor treatment of Burkholderici infection wherein the composition does not further comprise an antigen that is pharmaceutically active in the prophylaxis and/or treatment of Burkholderia infection.
15. The pharmaceutical composition of claim 14 wherein the I3urkho/derici infection is B urkho Ideri a pseudonwllei and/or Bit,*fio/deria,iwllei.
16. The pharmaceutical composition of claim 14 for the prophylaxis or treatment of melioidosis.
17, The pharmaceutical composition of any one of claims 14 to 16 wherein the phosphoantigen is IPP or CHDMAPP.
18. The pharmaceutical composition of any one of claims 14 to 16 wherein the phosphoantigen is BrHPP or IHPP.
19. Use of the composition recited in any one claims 14 to 18 for the manufacture of a medicament for the prophylax is and/or treatment of Burkhoideria infection.
20. The use of claim 19 wherein the Burkho/deria is I3urkholderiapseudoina/iei and/or Burkholderia rncii/ei. * . *S..... * S *S S * S S * .. S.. * S*....SS