GB2422903A - Method of analyzing protein - Google Patents

Method of analyzing protein Download PDF

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Publication number
GB2422903A
GB2422903A GB0611250A GB0611250A GB2422903A GB 2422903 A GB2422903 A GB 2422903A GB 0611250 A GB0611250 A GB 0611250A GB 0611250 A GB0611250 A GB 0611250A GB 2422903 A GB2422903 A GB 2422903A
Authority
GB
United Kingdom
Prior art keywords
amino acid
side chain
acid residue
specific amino
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0611250A
Other versions
GB0611250D0 (en
Inventor
Akira Tsugita
Keiichi Hosokawa
Takuji Nabetani
Kenichi Kamijo
Akira Saito
Kenji Miyazaki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tokyo Rikakikai Co Ltd
NEC Corp
Original Assignee
Tokyo Rikakikai Co Ltd
NEC Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tokyo Rikakikai Co Ltd, NEC Corp filed Critical Tokyo Rikakikai Co Ltd
Publication of GB0611250D0 publication Critical patent/GB0611250D0/en
Publication of GB2422903A publication Critical patent/GB2422903A/en
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G01N27/626—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using heat to ionise a gas
    • G01N27/628—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using heat to ionise a gas and a beam of energy, e.g. laser enhanced ionisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The presence or absence of a modifying group or the type of the modifying group at a specific amino acid residue in a protein is conveniently determined. A protein to be analyzed is treated with a reagent capable of labeling a side chain of a specific amino acid residue. In the case where the side chain of the specific amino acid residue is unmodified, the side chain is labeled (S101). Next, the protein, in which the side chain of the specific amino acid residue has been labeled, is specifically cleaved at a definite site (S102). Subsequently, the obtained peptide fragment is subjected to mass spectroscopy (S103). Based on the molecular weight data of the fragment thus obtained, the modified state of the side chain of the specific amino acid residue in the protein to be analyzed is analyzed (S104).

Description

GB 2422903 A continuation (72) Inventor(s): Akira Tsugita Keiichi Hosokawa
Takuji Nabetani Kenichi Kamijo Akira Saito Kenji Miyazaki (74) Agent and/or Address for Service: Reddie & Grose 16 Theobalds Road, LONDON, WC1X 8PL, United Kingdom
GB0611250A 2003-11-21 2004-11-19 Method of analyzing protein Withdrawn GB2422903A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2003391775 2003-11-21
JP2004093302 2004-03-26
PCT/JP2004/017275 WO2005049636A2 (en) 2003-11-21 2004-11-19 Method of analyzing protein

Publications (2)

Publication Number Publication Date
GB0611250D0 GB0611250D0 (en) 2006-07-19
GB2422903A true GB2422903A (en) 2006-08-09

Family

ID=34622193

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0611250A Withdrawn GB2422903A (en) 2003-11-21 2004-11-19 Method of analyzing protein

Country Status (3)

Country Link
JP (1) JPWO2005049636A1 (en)
GB (1) GB2422903A (en)
WO (1) WO2005049636A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9662308B2 (en) 2008-02-01 2017-05-30 Dritte Patentportfolio Beteiligungsgesellschaft Mbh & Co. Kg Orally bioavailable pentamidine prodrugs for the treatment of diseases
DE102008007381A1 (en) 2008-02-01 2009-08-13 Dritte Patentportfolio Beteiligungsgesellschaft Mbh & Co.Kg Amidines and guanidines and their derivatives for the treatment of diseases
CN105277712B (en) * 2014-07-25 2017-07-11 杭州景杰生物科技有限公司 A kind of method for identifying the modification of lysine ε amino side chains monomethylation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002189029A (en) * 2000-12-21 2002-07-05 Sumitomo Chem Co Ltd Determination method for amino acid sequence at n- terminal of protein
JP2002531810A (en) * 1998-11-24 2002-09-24 ゼリオン、ファーマスーティカルズ、ゲゼルシャフト、ミット、ベシュレンクテル、ハフツング Methods for modifying and identifying functional sites in proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002531810A (en) * 1998-11-24 2002-09-24 ゼリオン、ファーマスーティカルズ、ゲゼルシャフト、ミット、ベシュレンクテル、ハフツング Methods for modifying and identifying functional sites in proteins
JP2002189029A (en) * 2000-12-21 2002-07-05 Sumitomo Chem Co Ltd Determination method for amino acid sequence at n- terminal of protein

Also Published As

Publication number Publication date
GB0611250D0 (en) 2006-07-19
WO2005049636A3 (en) 2005-07-14
WO2005049636A2 (en) 2005-06-02
JPWO2005049636A1 (en) 2007-06-07

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