GB2422616A - Method and device for replicating arrays of cell colonies - Google Patents

Method and device for replicating arrays of cell colonies Download PDF

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Publication number
GB2422616A
GB2422616A GB0608336A GB0608336A GB2422616A GB 2422616 A GB2422616 A GB 2422616A GB 0608336 A GB0608336 A GB 0608336A GB 0608336 A GB0608336 A GB 0608336A GB 2422616 A GB2422616 A GB 2422616A
Authority
GB
United Kingdom
Prior art keywords
replicating
pad
colonies
pads
association
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0608336A
Other versions
GB0608336D0 (en
Inventor
Charles Boone
Pawel Kuzan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Engineering Services Inc
Original Assignee
Engineering Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/673,545 external-priority patent/US20050070011A1/en
Application filed by Engineering Services Inc filed Critical Engineering Services Inc
Publication of GB0608336D0 publication Critical patent/GB0608336D0/en
Publication of GB2422616A publication Critical patent/GB2422616A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06Plates; Walls; Drawers; Multilayer plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/26Inoculator or sampler
    • C12M1/263Replica plating devices
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/26Inoculator or sampler
    • C12M1/28Inoculator or sampler being part of container
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/04Flat or tray type, drawers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The replicating pad of the present invention has a generally planar body and a plurality of integrally formed pins extending downwardly from the body. Each pin corresponds to one of a plurality of samples. The replicating pads are adapted to be used to create a larger density array from a smaller density array. A plurality of pads, each having the same number of pins, are used in association with the same destination plate until a predetermined number of colonies are replicated. Thereafter a pad is used having the predetermined number of colonies. These steps may be repeated until a destination plate of the required density is created. In another aspect of the invention a replicating device is adapted to be used in association with a replicating pad having a plurality of pins extending downwardly.

Description

GB 2422616 A continuation (72) Inventor(s): Charles Boone Pawel Kuzan (74)
Agent and/or Address for Service: Alpha & Omega Chine Croft, East Hill, OTTERY St MARY, Devon, EX11 1PJ, United Kingdom
GB0608336A 2003-09-30 2004-09-30 Method and device for replicating arrays of cell colonies Withdrawn GB2422616A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/673,545 US20050070011A1 (en) 2003-09-30 2003-09-30 Method and device for replicating arrays of cell colonies
PCT/CA2004/001715 WO2005030922A1 (en) 2003-09-30 2004-09-30 Method and device for replicating arrays of cell colonies

Publications (2)

Publication Number Publication Date
GB0608336D0 GB0608336D0 (en) 2006-06-07
GB2422616A true GB2422616A (en) 2006-08-02

Family

ID=36589911

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0608336A Withdrawn GB2422616A (en) 2003-09-30 2004-09-30 Method and device for replicating arrays of cell colonies

Country Status (1)

Country Link
GB (1) GB2422616A (en)

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591567A (en) * 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
US4659673A (en) * 1985-11-01 1987-04-21 Brown Lewis R Replicator for cultures of microorganisms
US4717667A (en) * 1984-07-11 1988-01-05 Fmc Corporation Colony replicating device
US5061621A (en) * 1989-06-22 1991-10-29 Brandeis University Replica plating device with an integral marking element
US5587322A (en) * 1994-11-23 1996-12-24 Replicatech, Inc. Replica plating device
US6579499B1 (en) * 2000-05-31 2003-06-17 Autosplice, Inc. Liquid compound pin replicator with weight bias
US20030134428A1 (en) * 2002-01-14 2003-07-17 Shanler Michael S. Pin tool apparatus and method
US6610253B2 (en) * 2000-05-31 2003-08-26 Autosplice, Inc. Liquid pin transfer assembly with common pin bias
US6753180B1 (en) * 2002-01-11 2004-06-22 University Of South Florida Clonal replication system

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4591567A (en) * 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
US4717667A (en) * 1984-07-11 1988-01-05 Fmc Corporation Colony replicating device
US4659673A (en) * 1985-11-01 1987-04-21 Brown Lewis R Replicator for cultures of microorganisms
US5061621A (en) * 1989-06-22 1991-10-29 Brandeis University Replica plating device with an integral marking element
US5587322A (en) * 1994-11-23 1996-12-24 Replicatech, Inc. Replica plating device
US6579499B1 (en) * 2000-05-31 2003-06-17 Autosplice, Inc. Liquid compound pin replicator with weight bias
US6610253B2 (en) * 2000-05-31 2003-08-26 Autosplice, Inc. Liquid pin transfer assembly with common pin bias
US6753180B1 (en) * 2002-01-11 2004-06-22 University Of South Florida Clonal replication system
US20030134428A1 (en) * 2002-01-14 2003-07-17 Shanler Michael S. Pin tool apparatus and method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
http://www.vp-scientific.com/disposable_pin_tools.htm *

Also Published As

Publication number Publication date
GB0608336D0 (en) 2006-06-07

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Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)