GB2376948A - Design synthesis and use of affinity ligands - Google Patents

Design synthesis and use of affinity ligands Download PDF

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Publication number
GB2376948A
GB2376948A GB0223228A GB0223228A GB2376948A GB 2376948 A GB2376948 A GB 2376948A GB 0223228 A GB0223228 A GB 0223228A GB 0223228 A GB0223228 A GB 0223228A GB 2376948 A GB2376948 A GB 2376948A
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GB
United Kingdom
Prior art keywords
target
region
domain
affinity
ligand
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0223228A
Other versions
GB2376948B (en
GB0223228D0 (en
Inventor
Beining Chen
Richard Michael Day
Khalku Karim
Anthony Peter Francis Turner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cranfield University
Original Assignee
Cranfield University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cranfield University filed Critical Cranfield University
Publication of GB0223228D0 publication Critical patent/GB0223228D0/en
Publication of GB2376948A publication Critical patent/GB2376948A/en
Application granted granted Critical
Publication of GB2376948B publication Critical patent/GB2376948B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/64—Cyclic peptides containing only normal peptide links
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/30—Drug targeting using structural data; Docking or binding prediction
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

An affinity ligand is constructed from at least 3 domains: (1) a recognition domain, e.g. a 3-6 amino acid chain specific for a peptide region of a target; this may be discovered and/or optimised by fitting candidate structures or parts thereof to a model of the target in a computer memory; (2) an affinity domain, e.g. a domain capable of binding to a particular type of region of the target such as a glycosylation region; and (3) a flexible linker having a functional group enabling the ligand to be immobilised to a sensor surface to create a specific biosensor.

Description

(57) An affinity ligand is constructed from at least 3 domains: (1) a
recognition domain, e.g. a 3-6 amino acid chain specific for a peptide region of a target; this may be discovered and/or optimised by fitting candidate structures or parts thereof to a model of the target in a computer memory; (2) an affinity domain, e.g. a domain capable of binding to a particular type of region of the target such as a glycosylation region; and (3) a flexible linker having a functional group enabling the ligand to be immobilised to a sensor surface to create a specific biosensor.
GB0223228A 2000-03-08 2001-03-08 Design synthesis and use of affinity ligands Expired - Fee Related GB2376948B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0005469.2A GB0005469D0 (en) 2000-03-08 2000-03-08 A novel building block approach for designing affinity ligands for glycosylated haemoglobin HbA1c
PCT/GB2001/001011 WO2001066567A2 (en) 2000-03-08 2001-03-08 Design, synthesis and use of affinity ligands

Publications (3)

Publication Number Publication Date
GB0223228D0 GB0223228D0 (en) 2002-11-13
GB2376948A true GB2376948A (en) 2002-12-31
GB2376948B GB2376948B (en) 2004-09-29

Family

ID=9887130

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0005469.2A Ceased GB0005469D0 (en) 2000-03-08 2000-03-08 A novel building block approach for designing affinity ligands for glycosylated haemoglobin HbA1c
GB0223228A Expired - Fee Related GB2376948B (en) 2000-03-08 2001-03-08 Design synthesis and use of affinity ligands

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0005469.2A Ceased GB0005469D0 (en) 2000-03-08 2000-03-08 A novel building block approach for designing affinity ligands for glycosylated haemoglobin HbA1c

Country Status (3)

Country Link
AU (1) AU2001240783A1 (en)
GB (2) GB0005469D0 (en)
WO (1) WO2001066567A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005119255A2 (en) * 2004-05-12 2005-12-15 Applera Corporation Constrained cis-diol-borate bioconjugation system

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999021015A2 (en) * 1997-10-21 1999-04-29 Cranfield University Affinity ligands, their production and use

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999021015A2 (en) * 1997-10-21 1999-04-29 Cranfield University Affinity ligands, their production and use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Biochemical Society Transactions, Vol 19(4), 1991, Lewis et al, "Rational methods for site-directed drug...", pgs 883-887 *
Biosensors & Bioelectronics, Vol 13(7-8), 1998, Chen et al, "The synthesis and screeening of a...", pgs 779-785 *
Trends in Biotechnology, Vol 13(3), 1995, Bevan et al, "Identifying small-molecule lead...", pgs 115-121 *

Also Published As

Publication number Publication date
AU2001240783A1 (en) 2001-09-17
GB2376948B (en) 2004-09-29
GB0005469D0 (en) 2000-04-26
WO2001066567A3 (en) 2001-12-20
WO2001066567A2 (en) 2001-09-13
GB0223228D0 (en) 2002-11-13

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20090308